Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,852,854 G→T 100% P801P (CCG→CCT)  mutS → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,852,8540GT98.5% 866.9 / 2.6 268P801P (CCG→CCT) mutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (118/146);  total (122/146)
Fisher's exact test for biased strand distribution p-value = 4.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

GGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAA  >  CP009273/2852708‑2853000
                                                                                                                                                  |                                                                                                                                                  
ggCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTaa                                                                                                                                                  <  1:3705058/149‑1 (MQ=255)
ggCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTaa                                                                                                                                                  <  1:1659624/149‑1 (MQ=255)
 gCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAc                                                                                                                                                 <  1:1862661/149‑1 (MQ=255)
   gACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgc                                                                                                                                               <  1:91332/149‑1 (MQ=255)
    acacCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgcc                                                                                                                                              >  1:2035041/1‑149 (MQ=255)
     cacCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccg                                                                                                                                             >  2:1414266/1‑149 (MQ=255)
      acCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgc                                                                                                                                            >  2:3003795/1‑149 (MQ=255)
      acCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgc                                                                                                                                            <  2:596850/149‑1 (MQ=255)
       ccATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgcc                                                                                                                                           <  1:3714258/149‑1 (MQ=255)
         aTTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgccgc                                                                                                                                         <  1:3818748/149‑1 (MQ=255)
         aTTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgccgc                                                                                                                                         <  1:1775499/149‑1 (MQ=255)
         aTTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgccgc                                                                                                                                         <  2:2560561/149‑1 (MQ=255)
            gCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTAc                                                                                                                                      >  1:2356560/1‑149 (MQ=255)
             ccTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAATGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACg                                                                                                                                     >  1:3047831/1‑149 (MQ=255)
             ccTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGACAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACg                                                                                                                                     >  1:319855/1‑149 (MQ=255)
               tttATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCa                                                                                                                                   >  1:11349/1‑149 (MQ=255)
               tttATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCa                                                                                                                                   >  2:2591721/1‑149 (MQ=255)
               tttATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCa                                                                                                                                   >  1:10616/1‑149 (MQ=255)
                 tATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAg                                                                                                                                 >  1:3226300/1‑149 (MQ=255)
                   tGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTg                                                                                                                               >  1:3356819/1‑149 (MQ=255)
                   tGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTg                                                                                                                               >  1:3356668/1‑149 (MQ=255)
                    gCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTgg                                                                                                                              >  1:2559158/1‑149 (MQ=255)
                         gCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGt                                                                                                                         >  1:3854859/1‑149 (MQ=255)
                          cGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTa                                                                                                                        <  2:326634/149‑1 (MQ=255)
                          cGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTa                                                                                                                        <  2:778370/149‑1 (MQ=255)
                               aGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCaa                                                                                                                   >  2:3566888/1‑149 (MQ=255)
                                ggATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCaaa                                                                                                                  <  2:1860180/149‑1 (MQ=255)
                                 gATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCACGTGGATGGTACGCAAAt                                                                                                                 >  1:3319910/1‑149 (MQ=255)
                                 gATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAAt                                                                                                                 <  2:3719037/149‑1 (MQ=255)
                                 gATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAAt                                                                                                                 >  1:2731036/1‑149 (MQ=255)
                                 gATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAAt                                                                                                                 <  1:2882069/149‑1 (MQ=255)
                                     gcgcGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCt                                                                                                             >  1:2674997/1‑149 (MQ=255)
                                     gcgcGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCt                                                                                                             <  1:2338138/149‑1 (MQ=255)
                                      cgcgGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCtt                                                                                                            <  2:1719481/149‑1 (MQ=255)
                                         ggcgAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGc                                                                                                         <  2:792074/149‑1 (MQ=255)
                                          gcgAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCt                                                                                                        <  1:1235954/149‑1 (MQ=255)
                                          gcgAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCt                                                                                                        <  1:284482/149‑1 (MQ=255)
                                             aGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTc                                                                                                     <  2:2859678/149‑1 (MQ=255)
                                              gCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCa                                                                                                    >  2:2339969/1‑149 (MQ=255)
                                              gCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCa                                                                                                    >  2:2768645/1‑149 (MQ=255)
                                                 aaaGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTa                                                                                                 <  1:3093043/149‑1 (MQ=255)
                                                   aGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTAcc                                                                                               <  2:2060780/149‑1 (MQ=255)
                                                          gCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAgaagaa                                                                                        <  1:1419296/149‑1 (MQ=255)
                                                          gCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAgaagaa                                                                                        >  2:3200567/1‑149 (MQ=255)
                                                           ccTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGaaa                                                                                       <  2:1987378/149‑1 (MQ=255)
                                                           ccTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGaaa                                                                                       <  2:3730489/149‑1 (MQ=255)
                                                             tGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACt                                                                                     <  2:2015835/149‑1 (MQ=255)
                                                              ggCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACtt                                                                                    >  2:1153606/1‑149 (MQ=255)
                                                               gCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTc                                                                                   <  2:3919932/149‑1 (MQ=255)
                                                                  gTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGcc                                                                                >  1:1567515/1‑149 (MQ=255)
                                                                   ttGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCt                                                                               <  2:412357/149‑1 (MQ=255)
                                                                    tGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTg                                                                              <  2:2387010/149‑1 (MQ=255)
                                                                     gCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGc                                                                             >  1:3915793/1‑149 (MQ=255)
                                                                         ctctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTc                                                                         <  1:278697/149‑1 (MQ=255)
                                                                           ctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGa                                                                       >  1:1404317/1‑149 (MQ=255)
                                                                                aGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGctc                                                                  <  2:4094481/149‑1 (MQ=255)
                                                                                 ggCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGctct                                                                 <  2:106275/149‑1 (MQ=255)
                                                                                 ggCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGctct                                                                 >  2:1522892/1‑149 (MQ=255)
                                                                                 ggCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGctct                                                                 >  1:2641512/1‑149 (MQ=255)
                                                                                   cGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTgg                                                               <  2:3592296/149‑1 (MQ=255)
                                                                                   cGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTgg                                                               <  1:3645889/149‑1 (MQ=255)
                                                                                      gCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaa                                                            >  1:2918352/1‑149 (MQ=255)
                                                                                         aaaaGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATc                                                         <  1:3211760/149‑1 (MQ=255)
                                                                                         aaaaGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATc                                                         <  1:3735666/149‑1 (MQ=255)
                                                                                           aaGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCtt                                                       >  1:1462187/1‑149 (MQ=255)
                                                                                             gagGTTATTAATCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCTCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGa                                                     >  1:2437899/1‑149 (MQ=255)
                                                                                              agGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGATAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAATTGTCTTTTCTGTCAGTACCAGAAGTAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGAt                                                    <  1:2804217/149‑1 (MQ=255)
                                                                                               ggTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                   >  1:3473673/1‑149 (MQ=255)
                                                                                                    ttaAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGAt                                              >  2:1267527/1‑149 (MQ=255)
                                                                                                    ttaAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGAt                                              >  1:175845/1‑149 (MQ=255)
                                                                                                     taAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGAtt                                             <  1:1241436/149‑1 (MQ=255)
                                                                                                      aaGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtc                                            >  2:2636915/1‑149 (MQ=255)
                                                                                                       aGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCACCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtca                                           >  2:3853536/1‑149 (MQ=255)
                                                                                                         cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtcact                                         <  2:1791881/149‑1 (MQ=255)
                                                                                                           cgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtcactca                                       <  1:2663248/149‑1 (MQ=255)
                                                                                                              aCGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAACATCTTGATCCGGATTCACTCAccc                                    >  1:2513003/1‑149 (MQ=255)
                                                                                                                ggCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcg                                  >  1:3997800/1‑149 (MQ=255)
                                                                                                                 gCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcgc                                 >  1:3558171/1‑149 (MQ=255)
                                                                                                                 gCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACACcgc                                 >  2:1100727/1‑149 (MQ=255)
                                                                                                                    aaaGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTc                              >  2:3142952/1‑149 (MQ=255)
                                                                                                                        cTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgc                          <  2:245626/149‑1 (MQ=255)
                                                                                                                        cTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgc                          <  2:243921/149‑1 (MQ=255)
                                                                                                                          gCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgcgc                        >  2:3182976/1‑149 (MQ=255)
                                                                                                                           cGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCt                       <  1:373809/149‑1 (MQ=255)
                                                                                                                                gCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGt                  >  1:1919361/1‑149 (MQ=255)
                                                                                                                                gCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGt                  >  2:698366/1‑149 (MQ=255)
                                                                                                                                 cTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTg                 <  2:883820/149‑1 (MQ=255)
                                                                                                                                  tGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTgg                >  1:705791/1‑149 (MQ=255)
                                                                                                                                  tGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTgg                >  1:2454474/1‑149 (MQ=255)
                                                                                                                                   ggAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGa               <  2:2356560/149‑1 (MQ=255)
                                                                                                                                     aaaGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAtt             <  2:3695880/149‑1 (MQ=255)
                                                                                                                                     aaaGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAtt             >  1:463306/1‑149 (MQ=255)
                                                                                                                                      aaGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAttt            >  2:1963182/1‑149 (MQ=255)
                                                                                                                                       aGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTa           <  1:2961277/149‑1 (MQ=255)
                                                                                                                                        gCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTAt          <  2:3047831/149‑1 (MQ=255)
                                                                                                                                          aTTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCg        >  2:1763875/1‑149 (MQ=255)
                                                                                                                                          aTTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCg        >  1:1323838/1‑149 (MQ=255)
                                                                                                                                           tttCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGc       >  2:1272984/1‑149 (MQ=255)
                                                                                                                                             tCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCtt     <  2:3849691/149‑1 (MQ=255)
                                                                                                                                                ccGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa  >  2:3529596/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAA  >  CP009273/2852708‑2853000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑