Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,884,385 G→T 100% V291V (GTC→GTA)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,884,3850GT98.4% 616.6 / 0.8 189V291V (GTC→GTA) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (90/96);  total (92/97)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 189 total aligned reads displayed.

GTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTAA  >  CP009273/2884239‑2884530
                                                                                                                                                  |                                                                                                                                                 
gtTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTAc                                                                                                                                                 >  2:1780701/1‑149 (MQ=255)
gtTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTAc                                                                                                                                                 >  1:4074949/1‑149 (MQ=255)
   tCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAg                                                                                                                                              >  1:3063097/1‑149 (MQ=255)
   tCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAg                                                                                                                                              >  1:1251539/1‑149 (MQ=255)
   tCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGATTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAg                                                                                                                                              >  2:1099977/1‑149 (MQ=255)
    cACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGt                                                                                                                                             >  1:1294299/1‑149 (MQ=255)
     aCTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGtt                                                                                                                                            >  2:4088464/1‑149 (MQ=255)
      cTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTc                                                                                                                                           >  1:3245441/1‑149 (MQ=255)
       tGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCt                                                                                                                                          <  1:322123/149‑1 (MQ=255)
        gCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCtt                                                                                                                                         <  1:958851/149‑1 (MQ=255)
          gggTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTc                                                                                                                                       <  1:2590467/149‑1 (MQ=255)
           ggTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCa                                                                                                                                      <  2:3131153/149‑1 (MQ=255)
           ggTAAGCGTGGCGTAATCCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACTTTTTGCCATCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCa                                                                                                                                      >  1:3002654/1‑149 (MQ=255)
            gTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCAc                                                                                                                                     <  2:3975969/149‑1 (MQ=255)
            gTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCAc                                                                                                                                     >  2:938867/1‑149 (MQ=255)
             tAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCTCCTTTCAGCCACACCAGTTCTACAAGTTATTTCAcc                                                                                                                                    >  2:2227312/1‑149 (MQ=255)
                  gTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGc                                                                                                                               <  1:1634055/149‑1 (MQ=255)
                  gTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGc                                                                                                                               >  2:3222970/1‑149 (MQ=255)
                   tGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGcc                                                                                                                              >  1:3711014/1‑149 (MQ=255)
                     gCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCgg                                                                                                                            >  2:1173021/1‑149 (MQ=255)
                      cGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGa                                                                                                                           <  1:3317572/149‑1 (MQ=255)
                      cGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGa                                                                                                                           <  2:3009942/149‑1 (MQ=255)
                       gTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGAt                                                                                                                          >  2:3998316/1‑149 (MQ=255)
                       gTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGAt                                                                                                                          >  2:1963340/1‑149 (MQ=255)
                          aTTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGt                                                                                                                       >  2:1333052/1‑149 (MQ=255)
                           ttCTCAACACTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGtt                                                                                                                      <  2:3615746/149‑1 (MQ=255)
                           ttCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGtt                                                                                                                      >  1:895497/1‑149 (MQ=255)
                                 acaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATa                                                                                                                <  2:4086146/149‑1 (MQ=255)
                                 acaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATa                                                                                                                >  1:949530/1‑149 (MQ=255)
                                    aTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCa                                                                                                             >  2:3927600/1‑149 (MQ=255)
                                    aTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCa                                                                                                             >  2:2803409/1‑149 (MQ=255)
                                              tgtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAgc                                                                                                   <  1:89296/149‑1 (MQ=255)
                                              tgtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAgc                                                                                                   >  1:4046816/1‑149 (MQ=255)
                                               gtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAgcg                                                                                                  >  2:3920773/1‑149 (MQ=255)
                                                   aCGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTc                                                                                              >  1:855931/1‑149 (MQ=255)
                                                   aCGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTc                                                                                              <  2:1934179/149‑1 (MQ=255)
                                                      gTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAcc                                                                                           >  2:3757030/1‑149 (MQ=255)
                                                      gTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAcc                                                                                           >  2:2680465/1‑149 (MQ=255)
                                                      gTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAcc                                                                                           >  2:1667988/1‑149 (MQ=255)
                                                       tCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAccc                                                                                          >  2:296441/1‑149 (MQ=255)
                                                       tCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAccc                                                                                          <  2:1053551/149‑1 (MQ=255)
                                                              gAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGt                                                                                   >  1:1712481/1‑149 (MQ=255)
                                                               aaGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTa                                                                                  >  1:1979297/1‑149 (MQ=255)
                                                                aGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTaa                                                                                 <  2:550851/149‑1 (MQ=255)
                                                                aGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTaa                                                                                 >  1:171703/1‑149 (MQ=255)
                                                                aGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTaa                                                                                 <  2:99484/149‑1 (MQ=255)
                                                                aGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTaa                                                                                 >  2:1288766/1‑149 (MQ=255)
                                                                 gTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAAc                                                                                >  1:3034716/1‑149 (MQ=255)
                                                                 gTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAAc                                                                                >  1:1042849/1‑149 (MQ=255)
                                                                    ccACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGAAAGGTTCATCGCCTTTCAGCCACAGAAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCa                                                                             >  2:894226/1‑149 (MQ=255)
                                                                     cACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCAt                                                                            <  2:4074949/149‑1 (MQ=255)
                                                                         gTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGccc                                                                        >  2:1930099/1‑149 (MQ=255)
                                                                            gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                     >  1:1091304/1‑149 (MQ=255)
                                                                            gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                     <  1:2803409/149‑1 (MQ=255)
                                                                              gcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTc                                                                   <  1:1780701/149‑1 (MQ=255)
                                                                               cTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCa                                                                  <  1:3169052/149‑1 (MQ=255)
                                                                                    ttCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTaa                                                             >  2:1710578/1‑149 (MQ=255)
                                                                                         agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                        <  1:1121984/149‑1 (MQ=255)
                                                                                         agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                        <  2:282042/149‑1 (MQ=255)
                                                                                         agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                        <  2:281257/149‑1 (MQ=255)
                                                                                          ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAt                                                       <  2:1224250/149‑1 (MQ=255)
                                                                                          ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAt                                                       <  1:1142049/149‑1 (MQ=255)
                                                                                           gCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAtt                                                      <  1:686812/149‑1 (MQ=255)
                                                                                                gTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGat                                                 <  2:2296222/149‑1 (MQ=255)
                                                                                                 ttttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGata                                                <  1:270443/149‑1 (MQ=255)
                                                                                                  tttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCACTTTCGatat                                               >  1:3126969/1‑149 (MQ=255)
                                                                                                  tttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGatat                                               <  1:4074115/149‑1 (MQ=255)
                                                                                                   ttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATg                                              <  1:2676269/149‑1 (MQ=255)
                                                                                                        cTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTAACCTAAGTCAATTTCGATATGGCGaa                                         <  2:2045975/149‑1 (MQ=255)
                                                                                                         tCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAAc                                        >  2:1070788/1‑149 (MQ=255)
                                                                                                          cgtcGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACg                                       <  1:3628516/146‑1 (MQ=255)
                                                                                                          cGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACg                                       <  1:2997244/149‑1 (MQ=255)
                                                                                                          cGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACg                                       <  1:3276018/149‑1 (MQ=255)
                                                                                                          cGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACg                                       <  1:938867/149‑1 (MQ=255)
                                                                                                          cGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACg                                       <  1:1581692/149‑1 (MQ=255)
                                                                                                             cGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCt                                    >  1:2324715/1‑149 (MQ=255)
                                                                                                             cGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCt                                    >  2:1025358/1‑149 (MQ=255)
                                                                                                               gTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCttt                                  <  1:2894322/149‑1 (MQ=255)
                                                                                                                tGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCtttt                                 <  2:855931/149‑1 (MQ=255)
                                                                                                                 gACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGCTCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATTGCGAACGTCttttt                                <  2:2415620/149‑1 (MQ=255)
                                                                                                                  aCAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTc                               >  1:74186/1‑149 (MQ=255)
                                                                                                                      gTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCagag                           <  2:3314901/149‑1 (MQ=255)
                                                                                                                       ttCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGt                          >  2:322919/1‑149 (MQ=255)
                                                                                                                           tCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACg                      >  1:2790995/1‑149 (MQ=255)
                                                                                                                             gCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGcc                    <  2:332420/149‑1 (MQ=255)
                                                                                                                             gCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGcc                    <  2:333620/149‑1 (MQ=255)
                                                                                                                              ccTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGccc                   <  1:4061639/149‑1 (MQ=255)
                                                                                                                               cTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCg                  >  1:3427756/1‑149 (MQ=255)
                                                                                                                                 ttCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGTCGAACGTCTTTTTCAGAGTTACGCCCGGt                <  2:2503572/149‑1 (MQ=255)
                                                                                                                                  tCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTa               >  2:1449845/1‑149 (MQ=255)
                                                                                                                                  tCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTa               >  2:2754910/1‑149 (MQ=255)
                                                                                                                                    aGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAt             <  1:4029366/149‑1 (MQ=255)
                                                                                                                                        acaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTc         >  2:3682103/1‑149 (MQ=255)
                                                                                                                                         cagcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCt        <  2:1183452/149‑1 (MQ=255)
                                                                                                                                          agcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTg       >  1:800012/1‑149 (MQ=255)
                                                                                                                                          agcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTg       >  1:1510894/1‑149 (MQ=255)
                                                                                                                                           gcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTgg      <  2:840107/149‑1 (MQ=255)
                                                                                                                                           gcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTgg      <  2:840202/149‑1 (MQ=255)
                                                                                                                                            cagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGt     <  2:1568256/149‑1 (MQ=255)
                                                                                                                                               ttCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTaa  >  1:3800058/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTAA  >  CP009273/2884239‑2884530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑