Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,889,129 C→A 100% A4S (GCA→TCA)  ygcQ ← putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,889,1290CA97.1% 653.9 / 7.3 208A4S (GCA→TCA) ygcQputative flavoprotein
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (79/123);  total (82/126)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 208 total aligned reads displayed.

CACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGG  >  CP009273/2888984‑2889274
                                                                                                                                                 |                                                                                                                                                 
  cTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGatat                                                                                                                                              <  1:229895/149‑1 (MQ=255)
     ttCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCa                                                                                                                                           <  1:961734/149‑1 (MQ=255)
     ttCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCa                                                                                                                                           <  1:2949395/149‑1 (MQ=255)
       ccaccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATg                                                                                                                                         <  1:2490832/149‑1 (MQ=255)
       ccaccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATg                                                                                                                                         <  1:420418/149‑1 (MQ=255)
         accaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGc                                                                                                                                       <  1:2778900/149‑1 (MQ=255)
         accaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGc                                                                                                                                       >  2:1208051/1‑149 (MQ=255)
          ccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCt                                                                                                                                      <  1:1735071/149‑1 (MQ=255)
          ccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCt                                                                                                                                      <  1:1921627/149‑1 (MQ=255)
             aCGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCa                                                                                                                                   >  2:2649111/1‑149 (MQ=255)
                 aTCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTNCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCt                                                                                                                               <  1:2084362/149‑1 (MQ=255)
                 aTCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATccn                                                                                                                               <  1:1033132/149‑2 (MQ=255)
                 aTCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCt                                                                                                                               <  1:1030475/149‑1 (MQ=255)
                  tCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCtt                                                                                                                              <  2:401052/149‑1 (MQ=255)
                   ccAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCttt                                                                                                                             <  2:246317/149‑1 (MQ=255)
                        aCCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGt                                                                                                                        <  1:3672195/149‑1 (MQ=255)
                          cTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTaa                                                                                                                      <  2:418725/149‑1 (MQ=255)
                            gTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAAt                                                                                                                    <  1:3003826/149‑1 (MQ=255)
                             ttCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATa                                                                                                                   <  1:2421465/149‑1 (MQ=255)
                                cGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                                                                <  2:2500399/149‑1 (MQ=255)
                                 gCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTcc                                                                                                               <  1:2120481/149‑1 (MQ=255)
                                  ccACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCt                                                                                                              >  2:189985/1‑149 (MQ=255)
                                  ccACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCt                                                                                                              <  2:3077252/149‑1 (MQ=255)
                                    aCTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGc                                                                                                            <  1:3053656/149‑1 (MQ=255)
                                    aCTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGc                                                                                                            >  1:323822/1‑149 (MQ=255)
                                    aCTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGc                                                                                                            >  2:1650953/1‑149 (MQ=255)
                                     cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGcc                                                                                                           <  1:2866528/149‑1 (MQ=255)
                                     cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGcc                                                                                                           >  1:2595965/1‑149 (MQ=255)
                                     cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGcc                                                                                                           >  1:2596468/1‑149 (MQ=255)
                                      tACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCa                                                                                                          <  1:565076/149‑1 (MQ=255)
                                        cAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATa                                                                                                        <  2:2920854/149‑1 (MQ=255)
                                              gAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCtttt                                                                                                  >  1:1994862/1‑149 (MQ=255)
                                              gAGGTTCAATCTGCCAGTGCGCCAGGGTAAAACCGCTGAAATCCTGCGCAGCCAGCCAGCTTGCTTTTGCGTCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCtttt                                                                                                  <  1:3668870/149‑1 (MQ=255)
                                                ggTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGa                                                                                                >  1:2315779/1‑149 (MQ=255)
                                                ggTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGa                                                                                                <  2:2595965/149‑1 (MQ=255)
                                                ggTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGa                                                                                                <  2:3302742/149‑1 (MQ=255)
                                                ggTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGa                                                                                                <  1:3407566/149‑1 (MQ=255)
                                                  ttCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGc                                                                                              <  1:1544680/149‑1 (MQ=255)
                                                   tCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCt                                                                                             <  2:1834653/149‑1 (MQ=255)
                                                   tCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCt                                                                                             <  2:3136174/149‑1 (MQ=255)
                                                   tCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCt                                                                                             <  1:1920465/149‑1 (MQ=255)
                                                    cAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCtt                                                                                            <  1:2739196/149‑1 (MQ=255)
                                                    cAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCtt                                                                                            >  2:632030/1‑149 (MQ=255)
                                                    cAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCtt                                                                                            <  1:3841910/149‑1 (MQ=255)
                                                    cAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCtt                                                                                            <  1:3841965/149‑1 (MQ=255)
                                                      aTCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCtttt                                                                                          >  2:2135730/1‑149 (MQ=255)
                                                      aTCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCtttt                                                                                          <  2:4005672/149‑1 (MQ=255)
                                                       tCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCttttt                                                                                         >  2:2110842/1‑149 (MQ=255)
                                                         tGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCAATAGCTTTTGAGCTTTTTCt                                                                                       >  2:3948325/1‑149 (MQ=255)
                                                            cAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCt                                                                                    <  2:901010/149‑1 (MQ=255)
                                                             aGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTa                                                                                   >  2:3915413/1‑149 (MQ=255)
                                                              gTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTAc                                                                                  >  2:2152277/1‑149 (MQ=255)
                                                                  gcCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGtt                                                                              <  1:1094705/149‑1 (MQ=255)
                                                                      gggTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGcc                                                                          >  1:2204617/1‑149 (MQ=255)
                                                                            aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaa                                                                    <  1:89840/149‑1 (MQ=255)
                                                                            aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaa                                                                    <  1:90670/149‑1 (MQ=255)
                                                                            aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaa                                                                    <  2:744162/149‑1 (MQ=255)
                                                                            aaCCGCTGAAAACCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGACACAAATGAGATAGTCAAGGCTGCATCCTTTGGCGTAAATAGTCATGCCATAGCGTTTCAGCATATTAAGCTACCGATTGCCCatcaa                                                                    >  2:2411605/1‑149 (MQ=255)
                                                                              ccGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaatc                                                                  <  1:161245/149‑1 (MQ=255)
                                                                              ccGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaatc                                                                  <  2:187463/149‑1 (MQ=255)
                                                                                gCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAg                                                                >  2:271946/1‑149 (MQ=255)
                                                                                      aTCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGc                                                          >  1:1293935/1‑149 (MQ=255)
                                                                                         cTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTcc                                                       >  2:2613365/1‑149 (MQ=255)
                                                                                         cTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTcc                                                       <  1:1334017/149‑1 (MQ=255)
                                                                                         cTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTcc                                                       >  2:3562630/1‑149 (MQ=255)
                                                                                         cTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTcc                                                       <  1:2135730/149‑1 (MQ=255)
                                                                                                gccagccaGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGcgtcgt                                                <  2:2001124/149‑1 (MQ=255)
                                                                                                gccagccaGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGcgtcgt                                                <  2:2882957/149‑1 (MQ=255)
                                                                                                 ccagccagCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGtt                                               <  2:2838956/149‑1 (MQ=255)
                                                                                                          ttGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgctctgctc                                      <  2:1418942/149‑1 (MQ=255)
                                                                                                          ttGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgctctgctc                                      <  2:1502520/149‑1 (MQ=255)
                                                                                                             cTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTg                                   >  1:1766702/1‑149 (MQ=255)
                                                                                                              tATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGc                                  >  1:3547876/1‑149 (MQ=255)
                                                                                                               aTTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGcc                                 <  2:841466/149‑1 (MQ=255)
                                                                                                                ttGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCa                                <  1:796264/149‑1 (MQ=255)
                                                                                                                ttGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCa                                <  1:1540653/149‑1 (MQ=255)
                                                                                                                ttGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCa                                >  2:3599445/1‑149 (MQ=255)
                                                                                                                   cGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGtt                             <  2:2253489/149‑1 (MQ=255)
                                                                                                                       aTTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAg                         >  1:636244/1‑149 (MQ=255)
                                                                                                                       aTTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAg                         >  2:1321951/1‑149 (MQ=255)
                                                                                                                        ttCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGa                        >  2:1170102/1‑149 (MQ=255)
                                                                                                                        ttCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGa                        >  2:3652304/1‑149 (MQ=255)
                                                                                                                          ctcCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGaca                      >  1:1837089/1‑149 (MQ=255)
                                                                                                                           tcCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGacac                     >  2:47521/1‑149 (MQ=255)
                                                                                                                             cTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTg                   >  1:593361/1‑149 (MQ=255)
                                                                                                                              tGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGc                  <  1:2607456/149‑1 (MQ=255)
                                                                                                                               gATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCa                 >  1:2654814/1‑149 (MQ=255)
                                                                                                                                  ttATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCAtag              >  2:1590018/1‑147 (MQ=255)
                                                                                                                                   tATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGc             <  1:1777208/149‑1 (MQ=255)
                                                                                                                                   tATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGc             >  2:3065049/1‑149 (MQ=255)
                                                                                                                                    aTTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCt            <  2:536700/149‑1 (MQ=255)
                                                                                                                                     ttGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTg           <  1:1720237/149‑1 (MQ=255)
                                                                                                                                     ttGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTAGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTg           <  1:1180126/149‑1 (MQ=255)
                                                                                                                                      tGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTgg          >  1:2247189/1‑149 (MQ=255)
                                                                                                                                         cacaATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCAt       >  1:2512862/1‑149 (MQ=255)
                                                                                                                                         cacaATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCAt       >  1:49661/1‑149 (MQ=255)
                                                                                                                                          acaATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCAtt      >  2:1772028/1‑149 (MQ=255)
                                                                                                                                             aTTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCg   >  2:3813549/1‑149 (MQ=255)
                                                                                                                                             aTTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCAACGCTGGCATATCg   >  1:3165737/1‑149 (MQ=255)
                                                                                                                                              ttGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTAGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCgg  >  1:2534605/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGG  >  CP009273/2888984‑2889274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑