Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,902,302 C→A 25.1% D242Y (GAT→TAT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,902,3020CA25.1% 598.1 / 156.9 266D242Y (GAT→TAT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (95/104);  new base A (35/32);  total (130/136)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 268 total aligned reads displayed.

GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGC  >  CP009273/2902156‑2902450
                                                                                                                                                  |                                                                                                                                                    
gACTTCACGTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCAGTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGa                                                                                                                                                    <  2:2703180/149‑1 (MQ=255)
  cTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACg                                                                                                                                                  <  1:3226099/149‑1 (MQ=255)
   ttCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGt                                                                                                                                                 <  2:1389979/149‑1 (MQ=255)
     cACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCt                                                                                                                                               <  1:3160959/149‑1 (MQ=255)
        ttACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATATATGGAATCGACGTCTTTc                                                                                                                                            >  1:3502874/1‑149 (MQ=255)
        ttACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTc                                                                                                                                            <  2:6670/149‑1 (MQ=255)
        ttACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTc                                                                                                                                            <  2:1925411/149‑1 (MQ=255)
           ccGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGATTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCaga                                                                                                                                         <  2:3817847/149‑1 (MQ=255)
             gggTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAgaga                                                                                                                                       <  1:1295377/149‑1 (MQ=255)
                ttCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAAt                                                                                                                                    >  2:2265367/1‑149 (MQ=255)
                 tCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGTAATAGACGTCTTTCAGAGAAATa                                                                                                                                   <  2:2055885/149‑1 (MQ=255)
                 tCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATa                                                                                                                                   >  1:2222001/1‑149 (MQ=255)
                  cGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATaa                                                                                                                                  <  1:2393078/149‑1 (MQ=255)
                    cttcttCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAAcc                                                                                                                                <  2:3316406/149‑1 (MQ=255)
                      tcttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGc                                                                                                                              >  2:3213775/1‑149 (MQ=255)
                        ttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                            >  1:1411400/1‑149 (MQ=255)
                        ttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                            >  1:3276691/1‑149 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCttt                                                                                                                           <  1:3387922/149‑1 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCttt                                                                                                                           <  1:2842942/149‑1 (MQ=255)
                           gAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCttttt                                                                                                                         <  1:2809750/149‑1 (MQ=255)
                             aGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTcc                                                                                                                       >  2:2753646/1‑149 (MQ=255)
                             aGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTAAGAGAAATAACCGCTTTTTcc                                                                                                                       <  1:179455/149‑1 (MQ=255)
                                  aCCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAAc                                                                                                                  <  1:1498971/149‑1 (MQ=255)
                                   ccTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACa                                                                                                                 <  1:14197/149‑1 (MQ=255)
                                    cTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt                                                                                                                >  2:2932235/1‑149 (MQ=255)
                                    cTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt                                                                                                                <  2:1304694/149‑1 (MQ=255)
                                        tCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACa                                                                                                            <  1:250605/149‑1 (MQ=255)
                                        tCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACa                                                                                                            <  1:472477/149‑1 (MQ=255)
                                        tCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACa                                                                                                            <  1:539818/149‑1 (MQ=255)
                                         cccATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAg                                                                                                           >  1:1981637/1‑149 (MQ=255)
                                         cccATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAg                                                                                                           >  1:802047/1‑149 (MQ=255)
                                          ccATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGa                                                                                                          >  2:3679124/1‑149 (MQ=255)
                                          ccATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAAAATAATCGTCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAATACAGa                                                                                                          >  1:1107172/1‑149 (MQ=255)
                                           cATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGaa                                                                                                         <  1:840858/149‑1 (MQ=255)
                                            aTTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAAc                                                                                                        >  2:3143322/1‑149 (MQ=255)
                                              tCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACaa                                                                                                      <  1:1395260/149‑1 (MQ=255)
                                              tCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACaa                                                                                                      <  1:937617/149‑1 (MQ=255)
                                              tCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACaa                                                                                                      <  2:2854919/149‑1 (MQ=255)
                                               cGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAAt                                                                                                     <  1:2173280/149‑1 (MQ=255)
                                                  aCAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCa                                                                                                  >  2:1501808/1‑149 (MQ=255)
                                                    aGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAAt                                                                                                >  2:2907762/1‑149 (MQ=255)
                                                    aGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAAt                                                                                                >  2:1034816/1‑149 (MQ=255)
                                                     gATTCGCTCCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATc                                                                                               >  2:880669/1‑149 (MQ=255)
                                                      aTTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCt                                                                                              <  2:2464639/149‑1 (MQ=255)
                                                          gCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGc                                                                                          <  2:108306/149‑1 (MQ=255)
                                                            ttCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAc                                                                                        >  2:1046771/1‑149 (MQ=255)
                                                                gggCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGACTTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGTACATTACAGAACAATGCAATCTTCGCAtgttc                                                                                    >  1:3890181/1‑149 (MQ=255)
                                                                  gCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcgttcgt                                                                                  >  2:3398832/1‑149 (MQ=255)
                                                                     gTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGttcgttcg                                                                               >  2:395564/1‑149 (MQ=255)
                                                                      tttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGc                                                                              <  2:832149/149‑1 (MQ=255)
                                                                       ttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGcc                                                                             >  1:551051/1‑149 (MQ=255)
                                                                          aGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGa                                                                          >  1:1608978/1‑149 (MQ=255)
                                                                            cTGAATCGTTTACAACTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAAc                                                                        <  2:3453168/149‑1 (MQ=255)
                                                                               aaTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAgc                                                                     >  2:1654191/1‑149 (MQ=255)
                                                                               aaTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGATTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAgc                                                                     <  1:2114463/149‑1 (MQ=255)
                                                                               aaTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAAACGGCCCGGAATTTAATATATTGAATCGACGTATTTCAGAGAAATAACCGCTTTTTCCGGAACCTTACAGAACAATGCAATATTCGCACGTTCGATCGCCGGAACAgc                                                                     >  2:3849268/1‑149 (MQ=255)
                                                                                    tttACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGAt                                                                >  1:1780636/1‑149 (MQ=255)
                                                                                       aCAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTg                                                             >  1:2017427/1‑149 (MQ=255)
                                                                                        cAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGa                                                            <  2:2292425/149‑1 (MQ=255)
                                                                                         aaaTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCCATCTTCGCACGTTCGTTCTCCGGAACAGCGCGATCTGaa                                                           >  2:3087158/1‑149 (MQ=255)
                                                                                         aaaTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGaa                                                           >  2:2312699/1‑149 (MQ=255)
                                                                                            tataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGa                                                        <  2:284557/149‑1 (MQ=255)
                                                                                             ataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGAc                                                       >  2:1705081/1‑149 (MQ=255)
                                                                                              taATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACa                                                      >  1:876694/1‑149 (MQ=255)
                                                                                               aaTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                     >  1:1933917/1‑149 (MQ=255)
                                                                                               aaTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                     >  1:166112/1‑149 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaaa                                                    <  1:3588062/149‑1 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaaa                                                    >  1:1214870/1‑149 (MQ=255)
                                                                                                   gTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCa                                                 <  1:3728322/149‑1 (MQ=255)
                                                                                                    tCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAg                                                >  2:3604387/1‑149 (MQ=255)
                                                                                                     ccAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAgg                                               >  1:2770071/1‑149 (MQ=255)
                                                                                                       aGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGAt                                             <  2:4089433/149‑1 (MQ=255)
                                                                                                        gCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATg                                            >  2:1677290/1‑149 (MQ=255)
                                                                                                          cccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTc                                          >  1:1909424/1‑149 (MQ=255)
                                                                                                           ccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCa                                         >  2:2221233/1‑149 (MQ=255)
                                                                                                           ccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCa                                         >  2:185015/1‑149 (MQ=255)
                                                                                                            cTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAg                                        <  1:2912352/149‑1 (MQ=255)
                                                                                                               agaTTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCt                                     >  2:3658553/1‑149 (MQ=255)
                                                                                                                 aTTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTgg                                   >  2:2244734/1‑149 (MQ=255)
                                                                                                                  tttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                  >  1:4063932/1‑149 (MQ=255)
                                                                                                                  tttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                  >  1:865999/1‑149 (MQ=255)
                                                                                                                    tCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATa                                <  1:2800726/149‑1 (MQ=255)
                                                                                                                        cAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGa                            <  1:3621147/149‑1 (MQ=255)
                                                                                                                             ccGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgag                       <  1:2524623/149‑1 (MQ=255)
                                                                                                                             ccGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgag                       >  1:3670709/1‑149 (MQ=255)
                                                                                                                             ccGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgag                       >  1:595035/1‑149 (MQ=255)
                                                                                                                              cGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgaga                      <  2:115658/149‑1 (MQ=255)
                                                                                                                              cGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgaga                      <  1:3398832/149‑1 (MQ=255)
                                                                                                                               ggAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGagag                     <  1:1046771/149‑1 (MQ=255)
                                                                                                                               ggAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGagag                     >  2:3540312/1‑149 (MQ=255)
                                                                                                                                 aaTTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagca                   <  1:298075/149‑1 (MQ=255)
                                                                                                                                 aaTTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagca                   <  1:300312/149‑1 (MQ=255)
                                                                                                                                   ttttATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagcagc                 <  1:395549/149‑1 (MQ=255)
                                                                                                                                     ttATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGctc               <  1:1015390/149‑1 (MQ=255)
                                                                                                                                          gATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTa          <  1:1677290/149‑1 (MQ=255)
                                                                                                                                            tGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACa        >  1:667646/1‑149 (MQ=255)
                                                                                                                                              gAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACaga      <  2:2640208/149‑1 (MQ=255)
                                                                                                                                              gAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACaga      >  1:2921480/1‑149 (MQ=255)
                                                                                                                                                 tCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTg   >  2:551848/1‑149 (MQ=255)
                                                                                                                                                  cGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGc  <  2:3855183/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGC  >  CP009273/2902156‑2902450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑