Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 255,487 A→T 100% S109T (TCT→ACT)  phoE ← outer membrane phosphoporin protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273255,4870AT98.0% 696.9 / 2.1 215S109T (TCT→ACT) phoEouter membrane phosphoporin protein E
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base T (102/108);  total (105/110)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 215 total aligned reads displayed.

GGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAA  >  CP009273/255341‑255632
                                                                                                                                                  |                                                                                                                                                 
 gtgtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAcc                                                                                                                                                >  2:3262511/1‑149 (MQ=255)
   gtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCa                                                                                                                                              >  1:338551/1‑149 (MQ=255)
    ttCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCaa                                                                                                                                             >  1:612712/1‑149 (MQ=255)
     tCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCaaa                                                                                                                                            >  1:960620/1‑149 (MQ=255)
       cGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATc                                                                                                                                          <  1:1457681/149‑1 (MQ=255)
       cGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATc                                                                                                                                          <  1:2861744/149‑1 (MQ=255)
        gATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCt                                                                                                                                         >  2:3965218/1‑149 (MQ=255)
         aTACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGGTACGACCATAATCGAAAGTACCCAAATCtt                                                                                                                                        >  2:1729474/1‑149 (MQ=255)
          tACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATAGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCttt                                                                                                                                       >  1:437219/1‑149 (MQ=255)
           aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTta                                                                                                                                      <  1:279978/149‑1 (MQ=255)
           aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTta                                                                                                                                      >  1:2727666/1‑149 (MQ=255)
           aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTta                                                                                                                                      >  1:2208119/1‑149 (MQ=255)
            cGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtat                                                                                                                                     <  1:1663211/149‑1 (MQ=255)
             gTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtata                                                                                                                                    <  1:142561/149‑1 (MQ=255)
              tCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTatat                                                                                                                                   <  1:2460037/149‑1 (MQ=255)
                gCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATAttt                                                                                                                                 >  2:2343055/1‑149 (MQ=255)
                 ccAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                <  2:1254341/149‑1 (MQ=255)
                 ccAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                <  1:690519/149‑1 (MQ=255)
                 ccAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                <  1:3920442/149‑1 (MQ=255)
                 ccAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                <  1:264482/149‑1 (MQ=255)
                   aGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCaa                                                                                                                              <  1:861229/149‑1 (MQ=255)
                    gACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAc                                                                                                                             <  1:235612/149‑1 (MQ=255)
                     aCCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAcc                                                                                                                            >  2:1988215/1‑149 (MQ=255)
                           ggCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaa                                                                                                                      >  2:2507218/1‑149 (MQ=255)
                           ggCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaa                                                                                                                      >  2:2510302/1‑149 (MQ=255)
                                gTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAgag                                                                                                                 >  2:696228/1‑147 (MQ=255)
                                  ttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgag                                                                                                               <  2:318616/149‑1 (MQ=255)
                                   tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgaga                                                                                                              <  1:2752535/149‑1 (MQ=255)
                                   tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgaga                                                                                                              >  1:510901/1‑149 (MQ=255)
                                   tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgaga                                                                                                              <  2:3038200/149‑1 (MQ=255)
                                      tCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAcgc                                                                                                           >  1:2272642/1‑149 (MQ=255)
                                       cATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAcgcg                                                                                                          >  2:584785/1‑149 (MQ=255)
                                        aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGt                                                                                                         >  1:3068403/1‑149 (MQ=255)
                                        aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGt                                                                                                         >  1:3068467/1‑149 (MQ=255)
                                        aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGt                                                                                                         >  2:1379793/1‑149 (MQ=255)
                                         tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtt                                                                                                        <  2:2154620/149‑1 (MQ=255)
                                           aaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAACTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtttt                                                                                                      <  2:1568070/149‑1 (MQ=255)
                                           aaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtttt                                                                                                      >  2:1086210/1‑149 (MQ=255)
                                            aGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttttt                                                                                                     <  1:1988215/149‑1 (MQ=255)
                                              ttGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTg                                                                                                   <  2:1655392/149‑1 (MQ=255)
                                               tGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGc                                                                                                  <  1:3271961/149‑1 (MQ=255)
                                               tGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGc                                                                                                  <  2:2238931/149‑1 (MQ=255)
                                                gTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                 <  2:1573705/149‑1 (MQ=255)
                                                 tCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCtg                                                                                                >  1:2352494/1‑149 (MQ=255)
                                                    gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGc                                                                                             <  2:199766/149‑1 (MQ=255)
                                                    gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGc                                                                                             >  1:62197/1‑149 (MQ=255)
                                                          gcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATc                                                                                       <  2:3646149/149‑1 (MQ=255)
                                                            cGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCAc                                                                                     <  2:544457/149‑1 (MQ=255)
                                                              aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActc                                                                                   >  1:3642138/1‑149 (MQ=255)
                                                              aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActc                                                                                   <  2:1435286/149‑1 (MQ=255)
                                                              aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActc                                                                                   >  1:705059/1‑149 (MQ=255)
                                                              aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActc                                                                                   >  1:1801523/1‑149 (MQ=255)
                                                                gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActctc                                                                                 >  1:2742975/1‑149 (MQ=255)
                                                                gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActctc                                                                                 <  1:2614215/149‑1 (MQ=255)
                                                                 aaTCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActctct                                                                                >  2:3626918/1‑149 (MQ=255)
                                                                    cGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCt                                                                             >  1:1483769/1‑149 (MQ=255)
                                                                    cGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCt                                                                             >  1:4026925/1‑149 (MQ=255)
                                                                    cGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCt                                                                             <  1:125016/149‑1 (MQ=255)
                                                                     gCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCtt                                                                            <  1:509607/149‑1 (MQ=255)
                                                                       caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTtta                                                                          <  1:3285519/149‑1 (MQ=255)
                                                                        accaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttat                                                                         >  2:3861637/1‑149 (MQ=255)
                                                                         ccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttatt                                                                        <  2:2742147/149‑1 (MQ=255)
                                                                            aaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTAcc                                                                     >  2:1620926/1‑149 (MQ=255)
                                                                            aaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTAcc                                                                     >  2:2028109/1‑149 (MQ=255)
                                                                             aTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCg                                                                    >  1:1715291/1‑149 (MQ=255)
                                                                                   ggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAAc                                                              <  2:1814959/149‑1 (MQ=255)
                                                                                     aaCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActc                                                            <  1:1692657/149‑1 (MQ=255)
                                                                                     aaCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActc                                                            <  2:3199465/149‑1 (MQ=255)
                                                                                        atatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGc                                                         >  1:1740422/1‑149 (MQ=255)
                                                                                           tCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTc                                                      <  2:2272642/149‑1 (MQ=255)
                                                                                           tCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTc                                                      >  1:972116/1‑149 (MQ=255)
                                                                                            cGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTcc                                                     <  1:3207969/149‑1 (MQ=255)
                                                                                               tCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCaa                                                  >  1:2635730/1‑149 (MQ=255)
                                                                                                ccAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAAc                                                 <  2:2354308/149‑1 (MQ=255)
                                                                                                 cAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACg                                                >  1:4005883/1‑149 (MQ=255)
                                                                                                   ggCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGAc                                              >  2:1790261/1‑149 (MQ=255)
                                                                                                     cTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCCACGACCa                                            <  1:1772840/149‑1 (MQ=255)
                                                                                                      ttCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCAt                                           <  1:1001243/149‑1 (MQ=255)
                                                                                                       tCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATa                                          >  2:1074472/1‑149 (MQ=255)
                                                                                                             tCATACAACGCCCCCAGGTTACGACCATAATCGAATGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagt                                    <  2:3537196/149‑1 (MQ=255)
                                                                                                             tCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagt                                    <  2:1489114/149‑1 (MQ=255)
                                                                                                              cATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtc                                   <  2:1483769/149‑1 (MQ=255)
                                                                                                              cATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtc                                   >  2:3323584/1‑149 (MQ=255)
                                                                                                               aTACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtca                                  >  1:3769076/1‑149 (MQ=255)
                                                                                                               aTACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtca                                  >  2:3553152/1‑149 (MQ=255)
                                                                                                                  cAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGtt                               <  2:3977601/149‑1 (MQ=255)
                                                                                                                    aCGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGa                             <  2:2297033/149‑1 (MQ=255)
                                                                                                                           cAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTaa                      <  2:2626053/149‑1 (MQ=255)
                                                                                                                           cAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTaa                      <  1:1701093/149‑1 (MQ=255)
                                                                                                                              gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                   >  2:3974948/1‑149 (MQ=255)
                                                                                                                              gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                   >  2:2991500/1‑149 (MQ=255)
                                                                                                                              gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAtct                   >  1:698693/1‑147 (MQ=255)
                                                                                                                                    aCCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt             <  1:3869119/149‑1 (MQ=255)
                                                                                                                                    aCCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt             <  2:3062220/149‑1 (MQ=255)
                                                                                                                                     ccATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTc            <  1:2047366/149‑1 (MQ=255)
                                                                                                                                         aaTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCt        <  2:3068467/149‑1 (MQ=255)
                                                                                                                                         aaTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCt        <  2:3068403/149‑1 (MQ=255)
                                                                                                                                           tCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCttt      >  1:1543295/1‑149 (MQ=255)
                                                                                                                                            cGAAAGTACCCAAATCTTTATATTTCACCCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTg     <  1:3941065/149‑1 (MQ=255)
                                                                                                                                               aaGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGaaa  >  1:1382588/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAA  >  CP009273/255341‑255632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑