Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,918,415 G→A 100% P75P (CCC→CCT)  syd ← SecY‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,918,4150GA99.2% 741.6 / ‑2.4 242P75P (CCC→CCT) sydSecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (134/105);  total (134/108)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

TTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918269‑2918559
                                                                                                                                                  |                                                                                                                                                
ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTagg                                                                                                                                                <  1:3419481/149‑1 (MQ=255)
 tCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGt                                                                                                                                               <  1:4019944/149‑1 (MQ=255)
   tGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTg                                                                                                                                             <  2:3496229/149‑1 (MQ=255)
      gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTAt                                                                                                                                          <  2:3668371/149‑1 (MQ=255)
      gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTAt                                                                                                                                          <  1:582712/149‑1 (MQ=255)
        cACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATca                                                                                                                                        >  2:3836095/1‑149 (MQ=255)
            aGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAat                                                                                                                                    <  2:1484438/149‑1 (MQ=255)
            aGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAat                                                                                                                                    <  2:3358692/149‑1 (MQ=255)
            aGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAat                                                                                                                                    <  2:1222204/149‑1 (MQ=255)
              aTGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAatat                                                                                                                                  >  1:1742230/1‑149 (MQ=255)
               tGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATc                                                                                                                                 <  1:1062807/149‑1 (MQ=255)
                 aCCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaa                                                                                                                               <  2:2920158/149‑1 (MQ=255)
                  ccAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaaa                                                                                                                              <  1:1185095/149‑1 (MQ=255)
                   cAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaaaa                                                                                                                             <  2:3909735/149‑1 (MQ=255)
                   cAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaaaa                                                                                                                             >  2:1912801/1‑149 (MQ=255)
                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAgc                                                                                                                           >  1:2291152/1‑149 (MQ=255)
                         gATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGt                                                                                                                       <  2:2514663/149‑1 (MQ=255)
                           ttttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACCATATCAAAAGCGCGTTc                                                                                                                     >  2:1856759/1‑149 (MQ=255)
                           ttttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTc                                                                                                                     >  1:3646480/1‑149 (MQ=255)
                             ttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCAGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCaa                                                                                                                   <  2:2925292/149‑1 (MQ=255)
                              tCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAAc                                                                                                                  <  1:2918622/149‑1 (MQ=255)
                                   cACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGt                                                                                                             >  2:381760/1‑149 (MQ=255)
                                       cGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTAc                                                                                                         <  1:2149618/149‑1 (MQ=255)
                                        gACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACa                                                                                                        >  1:2384289/1‑149 (MQ=255)
                                        gACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACa                                                                                                        <  2:930846/149‑1 (MQ=255)
                                           ggAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAttt                                                                                                     >  2:1889007/1‑149 (MQ=255)
                                             aaGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTg                                                                                                   <  2:222527/149‑1 (MQ=255)
                                             aaGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTg                                                                                                   <  2:3393005/149‑1 (MQ=255)
                                              aGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGt                                                                                                  >  2:2516190/1‑149 (MQ=255)
                                              aGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGt                                                                                                  <  2:3367523/149‑1 (MQ=255)
                                                  gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTccc                                                                                              <  2:3766510/149‑1 (MQ=255)
                                                  gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTccc                                                                                              >  2:45504/1‑149 (MQ=255)
                                                  gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTccc                                                                                              >  2:605059/1‑149 (MQ=255)
                                                     ttCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCgg                                                                                           >  1:1872231/1‑149 (MQ=255)
                                                          tCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAAc                                                                                      >  1:3125109/1‑149 (MQ=255)
                                                            cAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACgg                                                                                    >  1:2525031/1‑149 (MQ=255)
                                                                 ttGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTggg                                                                               >  1:379176/1‑149 (MQ=255)
                                                                  tGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTgggg                                                                              <  2:2289886/149‑1 (MQ=255)
                                                                   gcagcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGt                                                                             >  1:4095786/1‑149 (MQ=255)
                                                                   gcagcaATGTAAGTTTTATATCGCCAAACTGCGCGTGCATATCCCCGGAAAACTTAGTCGTATAAAAGGTATGATTTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGt                                                                             <  1:3266954/149‑1 (MQ=255)
                                                                      gcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGc                                                                          >  1:2399693/1‑149 (MQ=255)
                                                                        aaTGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCa                                                                        <  2:3179338/149‑1 (MQ=255)
                                                                          tGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGt                                                                      >  2:363218/1‑149 (MQ=255)
                                                                           ttCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGAAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCTTTTACTTTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGta                                                                     <  1:3448139/148‑1 (MQ=255)
                                                                            tCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGtat                                                                    >  1:45122/1‑149 (MQ=255)
                                                                              aGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATAc                                                                  >  2:3424320/1‑149 (MQ=255)
                                                                                tttGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACgg                                                                >  1:3863250/1‑149 (MQ=255)
                                                                                   gATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCAt                                                             <  2:3546305/149‑1 (MQ=255)
                                                                                   gATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCAt                                                             <  2:1497949/149‑1 (MQ=255)
                                                                                   gATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCAt                                                             <  1:3735010/149‑1 (MQ=255)
                                                                                   gATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCAt                                                             >  1:1290790/1‑149 (MQ=255)
                                                                                    atatCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATc                                                            <  2:1692459/149‑1 (MQ=255)
                                                                                      atCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCtt                                                          >  2:2813479/1‑149 (MQ=255)
                                                                                      atCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCtt                                                          >  1:2677901/1‑149 (MQ=255)
                                                                                       tCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGTCAGTATACGGCATCTTc                                                         <  1:2522471/149‑1 (MQ=255)
                                                                                       tCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTc                                                         >  1:3984037/1‑149 (MQ=255)
                                                                                       tCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTc                                                         >  2:3759563/1‑149 (MQ=255)
                                                                                        cGCCAAACTGCTCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCg                                                        >  1:2207629/1‑149 (MQ=255)
                                                                                        cGCCAAACTGCTCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCg                                                        >  1:131176/1‑149 (MQ=255)
                                                                                         gCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCgg                                                       >  2:2659015/1‑149 (MQ=255)
                                                                                         gCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCgg                                                       <  2:2350412/149‑1 (MQ=255)
                                                                                          ccAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGt                                                      >  1:3619432/1‑149 (MQ=255)
                                                                                             aaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGt                                                   <  1:514905/149‑1 (MQ=255)
                                                                                             aaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGt                                                   <  1:1661539/149‑1 (MQ=255)
                                                                                              aCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGtt                                                  >  1:2774176/1‑149 (MQ=255)
                                                                                               atGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTg                                                 <  1:1693209/148‑1 (MQ=255)
                                                                                                tGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAACGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGa                                                <  1:1491517/149‑1 (MQ=255)
                                                                                                tGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGa                                                >  2:3375235/1‑149 (MQ=255)
                                                                                                tGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGa                                                >  2:921113/1‑149 (MQ=255)
                                                                                                  cgcgTGCATATCCCCGGCAAACTGAGTCGTAAAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGTTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGaaa                                              <  1:1918/149‑1 (MQ=255)
                                                                                                    cgTGCATATCCCCGGCAAATTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaata                                            >  2:1119876/1‑149 (MQ=255)
                                                                                                    cgTGCATATCCCCGGCAAATTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaata                                            >  2:1119666/1‑149 (MQ=255)
                                                                                                     gTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaataa                                           >  1:3909712/1‑149 (MQ=255)
                                                                                                      tGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaataat                                          <  2:2426470/149‑1 (MQ=255)
                                                                                                      tGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaataat                                          <  1:646594/149‑1 (MQ=255)
                                                                                                        cATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGc                                        <  2:1380053/149‑1 (MQ=255)
                                                                                                          tatCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCAc                                      <  1:3534575/149‑1 (MQ=255)
                                                                                                            tCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACgg                                    >  1:3302618/1‑149 (MQ=255)
                                                                                                            tCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACgg                                    >  1:1251863/1‑149 (MQ=255)
                                                                                                             ccccGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGt                                   >  1:977263/1‑149 (MQ=255)
                                                                                                                cGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGGTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGaa                                >  1:2792041/1‑149 (MQ=255)
                                                                                                                  gCAAACTTAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAgg                              <  2:3188999/149‑1 (MQ=255)
                                                                                                                   cAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGa                             <  1:4102873/149‑1 (MQ=255)
                                                                                                                       cTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAacac                         >  1:2062280/1‑149 (MQ=255)
                                                                                                                           gTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTa                     >  1:3231492/1‑149 (MQ=255)
                                                                                                                           gTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTa                     >  2:727726/1‑149 (MQ=255)
                                                                                                                            tCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTAc                    >  2:2843760/1‑149 (MQ=255)
                                                                                                                            tCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTAc                    <  2:714463/149‑1 (MQ=255)
                                                                                                                             cGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACa                   >  2:941623/1‑149 (MQ=255)
                                                                                                                             cGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACa                   <  1:2854415/149‑1 (MQ=255)
                                                                                                                               tataAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGACATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGt                 >  2:122784/1‑149 (MQ=255)
                                                                                                                                ataAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGtt                >  1:1434192/1‑149 (MQ=255)
                                                                                                                                  aaaaGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTcc              >  1:941941/1‑149 (MQ=255)
                                                                                                                                  aaaaGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTGGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTcc              <  1:1483360/149‑1 (MQ=255)
                                                                                                                                   aaaGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCt             >  2:2683315/1‑149 (MQ=255)
                                                                                                                                    aaGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTc            >  1:1036334/1‑149 (MQ=255)
                                                                                                                                      ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCAc          >  2:2729434/1‑149 (MQ=255)
                                                                                                                                       gTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACt         <  1:4028468/149‑1 (MQ=255)
                                                                                                                                           gAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAAc     >  2:4044582/1‑149 (MQ=255)
                                                                                                                                            aaTTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACg    >  1:823673/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918269‑2918559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑