Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,920,050 C→A 100% I128I (ATC→ATA)  ygdH → UPF0717 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,920,0500CA97.2% 797.5 / 11.2 254I128I (ATC→ATA) ygdHUPF0717 family protein
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (106/141);  total (109/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 261 total aligned reads displayed.

CCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTG  >  CP009273/2919904‑2920194
                                                                                                                                                  |                                                                                                                                                
   gAAGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTaa                                                                                                                                             <  1:1106577/149‑1 (MQ=255)
    aaGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACAtaactaac                                                                                                                                            >  1:1136928/1‑149 (MQ=255)
    aaGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACAtaactaac                                                                                                                                            >  1:48695/1‑149 (MQ=255)
    aaGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACAtaactaac                                                                                                                                            >  1:3307950/1‑149 (MQ=255)
     agagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAAcc                                                                                                                                           <  1:2460107/149‑1 (MQ=255)
     agagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAAcc                                                                                                                                           >  2:2669820/1‑149 (MQ=255)
      gagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCt                                                                                                                                          >  1:2416548/1‑149 (MQ=255)
      gagGCTTTTGTCTATGGGCGAATTATTCGAGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACTGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCt                                                                                                                                          >  1:2983511/1‑149 (MQ=255)
       agGCTTTTGTCTATGGGCTAATTATTCGCGCTTTGCAGGCCATCTTGTTCGCGGTCCTGAGTGACATTCTCTTCGTTTCCGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTg                                                                                                                                         <  2:1270803/149‑1 (MQ=255)
       agGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCTACTTGTTCGCGGTCCTGGGTTACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTg                                                                                                                                         <  1:2651241/149‑1 (MQ=255)
       agGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTg                                                                                                                                         >  2:2006420/1‑149 (MQ=255)
       agGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTg                                                                                                                                         <  1:1203199/149‑1 (MQ=255)
         gCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGt                                                                                                                                       >  2:2730831/1‑149 (MQ=255)
          cTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTc                                                                                                                                      <  2:3895517/149‑1 (MQ=255)
          cTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTc                                                                                                                                      <  1:2230298/149‑1 (MQ=255)
           ttttGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCt                                                                                                                                     >  2:3529632/1‑149 (MQ=255)
           ttttGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCt                                                                                                                                     <  1:853491/149‑1 (MQ=255)
             ttGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCttt                                                                                                                                   <  2:63577/149‑1 (MQ=255)
               gTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTc                                                                                                                                 <  1:358290/149‑1 (MQ=255)
               gTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTc                                                                                                                                 >  1:4061346/1‑149 (MQ=255)
                   aTGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATc                                                                                                                             <  1:1865786/149‑1 (MQ=255)
                   aTGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATc                                                                                                                             <  1:752825/149‑1 (MQ=255)
                    tGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAACCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCt                                                                                                                            <  1:2421500/149‑1 (MQ=255)
                         gAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGt                                                                                                                       >  2:2460022/1‑149 (MQ=255)
                            ttattCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAAc                                                                                                                    <  1:3808754/149‑1 (MQ=255)
                             tattCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACg                                                                                                                   >  2:452154/1‑149 (MQ=255)
                                tCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATATCTAACCTGGTCTTTTCCATCTTGCGTAACGCTc                                                                                                                <  1:281350/149‑1 (MQ=255)
                                  gcgcTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgc                                                                                                              >  2:1425868/1‑149 (MQ=255)
                                      tttGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATACCACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCgcgcgc                                                                                                          <  2:1081427/149‑1 (MQ=255)
                                      tttGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCgcgcgc                                                                                                          >  2:3082362/1‑149 (MQ=255)
                                           aGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCAt                                                                                                     <  1:3869701/149‑1 (MQ=255)
                                             gCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCAtgt                                                                                                   <  1:1395295/149‑1 (MQ=255)
                                               cAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTgg                                                                                                 >  1:4002974/1‑149 (MQ=255)
                                               cAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTgg                                                                                                 <  2:3562910/149‑1 (MQ=255)
                                                aaCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTggg                                                                                                <  2:1592432/149‑1 (MQ=255)
                                                aaCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCAAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTggg                                                                                                >  2:2615413/1‑149 (MQ=255)
                                                 aCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGt                                                                                               <  2:1521176/149‑1 (MQ=255)
                                                       tCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAgcg                                                                                         >  1:3855667/1‑149 (MQ=255)
                                                              ccTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAata                                                                                  >  1:3881222/1‑149 (MQ=255)
                                                                tGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtg                                                                                <  2:2422324/149‑1 (MQ=255)
                                                                tGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtg                                                                                <  2:3358330/149‑1 (MQ=255)
                                                                tGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtg                                                                                <  1:2958071/149‑1 (MQ=255)
                                                                   gTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtggtg                                                                             <  2:1385108/149‑1 (MQ=255)
                                                                   gTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTCAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtggtg                                                                             >  2:3643011/1‑149 (MQ=255)
                                                                       cATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTct                                                                         <  2:3922594/149‑1 (MQ=255)
                                                                         ttCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTctgc                                                                       <  2:1667292/149‑1 (MQ=255)
                                                                          tctcTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTctgct                                                                      >  1:2902782/1‑149 (MQ=255)
                                                                          tctcTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTctgct                                                                      <  2:1033294/149‑1 (MQ=255)
                                                                                    tACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCa                                                            >  2:2408957/1‑149 (MQ=255)
                                                                                     aCGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCAc                                                           >  2:2112271/1‑149 (MQ=255)
                                                                                           aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAtt                                                     <  1:1664182/149‑1 (MQ=255)
                                                                                           aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAtt                                                     <  2:381226/149‑1 (MQ=255)
                                                                                           aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAtt                                                     <  2:381184/149‑1 (MQ=255)
                                                                                           aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAtt                                                     >  1:714396/1‑149 (MQ=255)
                                                                                             aTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTaa                                                   >  2:136966/1‑149 (MQ=255)
                                                                                              tCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAAc                                                  >  1:536620/1‑149 (MQ=255)
                                                                                              cccATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAAc                                                  >  1:1311421/2‑149 (MQ=255)
                                                                                               ccATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACg                                                 <  1:3722507/149‑1 (MQ=255)
                                                                                                 aTAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaa                                               >  2:3960394/1‑149 (MQ=255)
                                                                                                 aTAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaa                                               >  1:841093/1‑149 (MQ=255)
                                                                                                 aTAACACCCTTCGTTTTCCCACCCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTGTCCATCGTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaa                                               <  2:2156773/149‑1 (MQ=255)
                                                                                                  tAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaaa                                              >  1:2457486/1‑149 (MQ=255)
                                                                                                   aaCACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaaaa                                             <  2:3667791/149‑1 (MQ=255)
                                                                                                    acacCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAAc                                            >  1:78033/1‑149 (MQ=255)
                                                                                                       ccGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAg                                         >  1:3488645/1‑149 (MQ=255)
                                                                                                        cgttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGt                                        <  2:3018463/149‑1 (MQ=255)
                                                                                                        cgttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGt                                        <  2:4080233/149‑1 (MQ=255)
                                                                                                        cgttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGt                                        <  2:1117386/149‑1 (MQ=255)
                                                                                                         gttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTa                                       <  2:533843/149‑1 (MQ=255)
                                                                                                         gttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTa                                       <  2:2601042/149‑1 (MQ=255)
                                                                                                          ttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTAt                                      >  1:1817890/1‑149 (MQ=255)
                                                                                                          ttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTAt                                      >  1:3528382/1‑149 (MQ=255)
                                                                                                            cgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTAttt                                    <  2:2983511/149‑1 (MQ=255)
                                                                                                             gttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTg                                   <  2:3307950/149‑1 (MQ=255)
                                                                                                              ttttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGt                                  <  1:2581986/149‑1 (MQ=255)
                                                                                                               tttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTa                                 <  1:117064/149‑1 (MQ=255)
                                                                                                                ttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTAt                                >  2:1806058/1‑149 (MQ=255)
                                                                                                                ttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTAt                                <  1:3082362/149‑1 (MQ=255)
                                                                                                                ttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTAt                                <  2:2803470/149‑1 (MQ=255)
                                                                                                                    cAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGccc                            <  1:563086/149‑1 (MQ=255)
                                                                                                                      aCCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgt                          >  1:3791153/1‑149 (MQ=255)
                                                                                                                         tGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgc                       >  1:867695/1‑149 (MQ=255)
                                                                                                                            aTCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgtc                    <  1:2006420/149‑1 (MQ=255)
                                                                                                                               tcGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGa                 >  2:1549780/1‑149 (MQ=255)
                                                                                                                               tcGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATCCCCCTCGCGCCGGa                 >  2:958537/1‑149 (MQ=255)
                                                                                                                               ncGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGa                 >  1:2721750/2‑149 (MQ=255)
                                                                                                                                cGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaa                <  1:1425868/149‑1 (MQ=255)
                                                                                                                                  aCAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAAc              <  1:2222233/149‑1 (MQ=255)
                                                                                                                                    aaCTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCa            <  2:827119/149‑1 (MQ=255)
                                                                                                                                     aCTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAg           >  1:1988172/1‑149 (MQ=255)
                                                                                                                                     aCTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAg           >  2:4090567/1‑149 (MQ=255)
                                                                                                                                       tCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt         <  2:4038059/149‑1 (MQ=255)
                                                                                                                                        ccGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTg        >  2:1489219/1‑149 (MQ=255)
                                                                                                                                         cGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTgg       <  2:1326455/149‑1 (MQ=255)
                                                                                                                                          gTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTggg      >  2:237434/1‑149 (MQ=255)
                                                                                                                                           tCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGc     >  2:1792881/1‑149 (MQ=255)
                                                                                                                                           tCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGc     >  2:741560/1‑149 (MQ=255)
                                                                                                                                             cacaTAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCt   >  2:3388290/1‑149 (MQ=255)
                                                                                                                                              acaTAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTg  >  1:443995/1‑149 (MQ=255)
                                                                                                                                              acaTAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTg  <  2:2529527/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTG  >  CP009273/2919904‑2920194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑