Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,932,835 C→T 100% R37C (CGC→TGC)  fucR → l‑fucose operon activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,932,8350CT97.1% 801.9 / 10.0 244R37C (CGC→TGC) fucRl‑fucose operon activator
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base T (115/121);  total (120/124)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 247 total aligned reads displayed.

TGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGC  >  CP009273/2932691‑2932979
                                                                                                                                                |                                                                                                                                                
   cgcgGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGAtct                                                                                                                                           <  1:11283/149‑1 (MQ=255)
     ggggcggTTATCTAATGATGGGGTTAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCa                                                                                                                                         <  1:3195056/148‑1 (MQ=255)
          ggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGaa                                                                                                                                    <  1:3444069/149‑1 (MQ=255)
          ggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGaa                                                                                                                                    <  1:3444112/149‑1 (MQ=255)
           gTTATCGAATGATAGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAAt                                                                                                                                   >  2:4031361/1‑149 (MQ=255)
            ttATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAAtt                                                                                                                                  >  1:2397947/1‑149 (MQ=255)
              aTCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTAc                                                                                                                                <  1:4092840/149‑1 (MQ=255)
                 gAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGa                                                                                                                             <  2:3595310/149‑1 (MQ=255)
                     gatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCa                                                                                                                         >  1:1265939/1‑149 (MQ=255)
                     gatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCa                                                                                                                         >  2:397150/1‑149 (MQ=255)
                      atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAg                                                                                                                        <  1:2036089/149‑1 (MQ=255)
                      atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAg                                                                                                                        <  1:4071829/149‑1 (MQ=255)
                         gggTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGt                                                                                                                     <  2:918222/149‑1 (MQ=255)
                           gTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTaa                                                                                                                   >  1:3835437/1‑149 (MQ=255)
                           gTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTaa                                                                                                                   >  1:3836224/1‑149 (MQ=255)
                              aaaaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAt                                                                                                                <  2:1570367/149‑1 (MQ=255)
                              aaaaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAt                                                                                                                <  2:135075/149‑1 (MQ=255)
                                   tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTgc                                                                                                           <  1:2168968/149‑1 (MQ=255)
                                        aaGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAAt                                                                                                      <  1:832046/149‑1 (MQ=255)
                                          gCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCa                                                                                                    <  2:2667183/149‑1 (MQ=255)
                                              cACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGa                                                                                                <  2:3343688/149‑1 (MQ=255)
                                              cACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGa                                                                                                <  1:777857/149‑1 (MQ=255)
                                               aCGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCACTCATGGAc                                                                                               >  2:872625/1‑149 (MQ=255)
                                                    aGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCt                                                                                          >  2:3848556/1‑149 (MQ=255)
                                                    aGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCt                                                                                          <  2:2282661/149‑1 (MQ=255)
                                                        aGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAat                                                                                      <  1:3060703/149‑1 (MQ=255)
                                                        aGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAat                                                                                      >  1:872485/1‑149 (MQ=255)
                                                            aTAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAatatat                                                                                  >  2:1157981/1‑149 (MQ=255)
                                                            aTAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAatatat                                                                                  <  1:3554440/149‑1 (MQ=255)
                                                            aTAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAatatat                                                                                  <  1:3554295/149‑1 (MQ=255)
                                                              aGTCGACCTGCTGCAGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATcc                                                                                <  2:656467/149‑1 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  2:2822115/1‑149 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  1:1015356/1‑149 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  2:254311/1‑149 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  1:1047033/1‑149 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  2:900/1‑149 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  1:3027089/1‑149 (MQ=255)
                                                               gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  1:2779392/1‑149 (MQ=255)
                                                                  gACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCg                                                                            >  2:3179863/1‑149 (MQ=255)
                                                                    ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTc                                                                          <  2:2422077/149‑1 (MQ=255)
                                                                      tgctgctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCaa                                                                        >  1:959034/1‑149 (MQ=255)
                                                                        ctgctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCaaaa                                                                      <  2:2399538/149‑1 (MQ=255)
                                                                          gctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTAGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATc                                                                    >  1:3093664/1‑149 (MQ=255)
                                                                             gAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAg                                                                 <  1:2952865/149‑1 (MQ=255)
                                                                               aCCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGAc                                                               >  1:1976336/1‑149 (MQ=255)
                                                                               aCCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGAc                                                               >  2:528138/1‑149 (MQ=255)
                                                                               aCCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGAc                                                               <  2:3261850/149‑1 (MQ=255)
                                                                                   tACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTg                                                           <  2:2762804/149‑1 (MQ=255)
                                                                                   tACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTg                                                           <  1:1592847/149‑1 (MQ=255)
                                                                                    aCCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTgg                                                          <  1:389686/149‑1 (MQ=255)
                                                                                     ccAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGc                                                         <  2:504851/149‑1 (MQ=255)
                                                                                     ccAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGc                                                         >  2:1094952/1‑149 (MQ=255)
                                                                                       aGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGa                                                       >  2:2157533/1‑149 (MQ=255)
                                                                                        gCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGAt                                                      <  1:3263244/149‑1 (MQ=255)
                                                                                           tGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATccc                                                   >  1:1664335/1‑149 (MQ=255)
                                                                                            gACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCt                                                  <  1:2310350/149‑1 (MQ=255)
                                                                                             aCCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCtt                                                 <  1:1422440/149‑1 (MQ=255)
                                                                                             aCCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCtt                                                 <  1:3470404/149‑1 (MQ=255)
                                                                                               cACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTc                                               <  1:2096808/149‑1 (MQ=255)
                                                                                                     aGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCa                                         >  2:1819836/1‑149 (MQ=255)
                                                                                                      tctctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAg                                        >  2:325854/2‑149 (MQ=255)
                                                                                                       ctctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAgg                                       <  2:312857/149‑1 (MQ=255)
                                                                                                        tctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGTGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGc                                      <  2:418251/149‑1 (MQ=255)
                                                                                                         ctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCt                                     <  2:2095073/149‑1 (MQ=255)
                                                                                                           ctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGa                                   <  1:870348/149‑1 (MQ=255)
                                                                                                           ctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGa                                   >  1:3139966/1‑149 (MQ=255)
                                                                                                              tGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGaaaa                                <  2:3644097/149‑1 (MQ=255)
                                                                                                              tGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGaaaa                                >  1:533098/1‑149 (MQ=255)
                                                                                                               gAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAg                               <  1:2773574/149‑1 (MQ=255)
                                                                                                                aaCAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGc                              >  2:2390567/1‑149 (MQ=255)
                                                                                                                  cAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCa                            <  1:1385685/149‑1 (MQ=255)
                                                                                                                   aGCTAAAGGTCAGTAAAGAAACCATTAGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAt                           <  2:1722243/149‑1 (MQ=255)
                                                                                                                    gCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAtt                          <  2:1185903/149‑1 (MQ=255)
                                                                                                                     cTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTa                         >  1:2614899/1‑149 (MQ=255)
                                                                                                                      tAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTAt                        >  2:2809066/1‑149 (MQ=255)
                                                                                                                      tAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTAt                        <  1:1876780/149‑1 (MQ=255)
                                                                                                                      tAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCATTCACATCAGGCTGAAAAGCCCTTAt                        >  2:767519/1‑149 (MQ=255)
                                                                                                                      tAAAGGTCAGTAAAGAAACCATTAGTTGAGATCTGAATGAATTTCAGACTCAGGGTAAAATTATGAGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGATGAAAATCCATTAt                        <  2:2749701/149‑1 (MQ=255)
                                                                                                                         aGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgcg                     >  1:3519518/1‑149 (MQ=255)
                                                                                                                          ggTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgcgc                    >  2:718554/1‑149 (MQ=255)
                                                                                                                              cggaaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTGACATCCGGCAGAAAAGCCATTATGCGCATaa                <  2:3919321/146‑1 (MQ=255)
                                                                                                                               gTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTTCGCAATCATGGACGAGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGACATTATGCGCATaaa               >  1:3692595/1‑149 (MQ=255)
                                                                                                                                tAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATACAg              >  2:3685546/1‑149 (MQ=255)
                                                                                                                                tAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAg              >  1:2374616/1‑149 (MQ=255)
                                                                                                                                 aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGc             <  1:1958457/149‑1 (MQ=255)
                                                                                                                                 aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGc             >  1:2503031/1‑149 (MQ=255)
                                                                                                                                   aGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           <  1:3475086/149‑1 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          <  2:4002070/149‑1 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          <  1:3693972/149‑1 (MQ=255)
                                                                                                                                    gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          >  2:3455795/1‑149 (MQ=255)
                                                                                                                                     aaaCCATTCGTTGCGATCTCAATGAATTACATACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGat         >  2:746597/1‑149 (MQ=255)
                                                                                                                                     aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGat         <  1:335056/149‑1 (MQ=255)
                                                                                                                                     aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGat         <  1:763035/149‑1 (MQ=255)
                                                                                                                                     aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGat         <  1:1244614/149‑1 (MQ=255)
                                                                                                                                      aaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        >  1:3952896/1‑149 (MQ=255)
                                                                                                                                      aaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        <  2:1466574/149‑1 (MQ=255)
                                                                                                                                      aaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        >  1:552971/1‑149 (MQ=255)
                                                                                                                                        ccATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATc      <  2:223019/149‑1 (MQ=255)
                                                                                                                                         cATTAGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcg     >  2:1637367/1‑149 (MQ=255)
                                                                                                                                            tcgttgCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGATAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcgc  <  2:927675/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGC  >  CP009273/2932691‑2932979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑