BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,940,854	G→T	6.0%	intergenic (+32/‑2)	metZ → / → metW	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,940,854	0	G	T	6.0%	466.7 / 6.4	134	intergenic (+32/‑2)	metZ/metW	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base G (43/82); new base T (5/4); total (48/86)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAT  >  CP009273/2940706‑2941002

tGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTg                                                                                                                                                      <  1:3351188/149‑1 (MQ=255)

 gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGa                                                                                                                                                     <  2:656575/149‑1 (MQ=255)

 gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGa                                                                                                                                                     <  2:3654845/149‑1 (MQ=255)

      aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAATTAAAGCAGTACGGTGACGCgg                                                                                                                                                >  2:4040570/1‑149 (MQ=255)

           gcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTAATAACTAAAAGCAGTACGGTGACGCGGGGTgc                                                                                                                                           >  2:2134917/1‑148 (MQ=17)

            cgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGa                                                                                                                                          <  2:2074240/149‑1 (MQ=255)

               gCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTTACGCGGGGTGGagca                                                                                                                                       <  1:2630134/149‑1 (MQ=255)

               gCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGGAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGagca                                                                                                                                       <  2:436406/149‑1 (MQ=255)

                 ggAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTAGAAGAAGTAAAGCATTACTTTGACGAGGGGTGGagcagc                                                                                                                                     >  2:623230/1‑149 (MQ=18)

                  gAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                                                    <  2:1072729/149‑1 (MQ=255)

                   aGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCt                                                                                                                                   <  1:2754871/149‑1 (MQ=255)

                   aGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTGGATGAAGTAAAGCCCTACCGTGACCCGGGGTGGCCCAGCCt                                                                                                                                   >  1:240804/1‑149 (MQ=17)

                     tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTTACAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                                                 <  2:2320841/149‑1 (MQ=255)

                      ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt                                                                                                                                <  2:1320047/149‑1 (MQ=255)

                         gTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCCTGGGAGc                                                                                                                             >  2:388082/1‑149 (MQ=255)

                         gTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGc                                                                                                                             >  1:817401/1‑149 (MQ=255)

                            cgcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtag                                                                                                                          <  1:533841/149‑3 (MQ=255)

                             gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgt                                                                                                                         <  2:236451/149‑1 (MQ=255)

                                tCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCTGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                                                                                      <  1:1775797/149‑1 (MQ=255)

                                tCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCAGAAGGTAGTCGTTTCAAATCCGGCCCCCGCAACCAAGAAAAGGTTGATTTACAAAAGCAGTAAGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                                                                                      >  1:2819617/1‑149 (MQ=11)

                                  cACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGc                                                                                                                    >  1:2274785/1‑149 (MQ=255)

                                       aCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATa                                                                                                               <  2:726916/149‑1 (MQ=40)

                                       aCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAGAACCCGAAGGTCGTCGGTTCTTATCCGGCACCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATa                                                                                                               <  2:2935955/149‑1 (MQ=255)

                                        cgcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATaa                                                                                                              <  2:3399981/149‑1 (MQ=40)

                                           ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAccc                                                                                                           <  1:212600/149‑1 (MQ=40)

                                            gggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                                                          >  2:2197113/1‑149 (MQ=40)

                                               tGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAg                                                                                                       <  1:10587/149‑1 (MQ=40)

                                                ggAGCAGACTTTTATCTCGTCTGTCTAATAACCCGAAGGTCGTCGGTTCAAATACGTACCCCGCAACCAATTATAATTTGATGAATTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAgg                                                                                                      <  1:1831118/149‑1 (MQ=11)

                                                 gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTCAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgt                                                                                                     <  2:1267403/149‑1 (MQ=255)

                                                   gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTTTGATCAAGAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                                                   >  1:3697550/1‑149 (MQ=255)

                                                     agcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgtc                                                                                                 <  1:831116/149‑1 (MQ=40)

                                                     agcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgtc                                                                                                 <  1:556468/149‑1 (MQ=40)

                                                      gcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATCGTAGCACGTCGGGGTCGTCACACGATGGacgtcg                                                                                                >  2:1915002/1‑149 (MQ=14)

                                                        cTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                                              >  2:2404293/1‑149 (MQ=40)

                                                        cTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                                              <  2:2178227/149‑1 (MQ=40)

                                                            tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAACTTTGCTGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaa                                                                                          <  1:2850679/149‑1 (MQ=255)

                                                            tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaa                                                                                          <  1:2850668/149‑1 (MQ=40)

                                                              gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCGGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAt                                                                                        >  2:3338003/1‑149 (MQ=255)

                                                               cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAACTTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                                                       <  1:1054073/149‑1 (MQ=38)

                                                               cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                                                       <  1:3050494/149‑1 (MQ=40)

                                                               cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                                                       <  1:3180128/149‑1 (MQ=40)

                                                               cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                                                       <  2:284820/149‑1 (MQ=40)

                                                                         tCAGAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCCACCAATTAAACTGTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGc                                                                             <  2:106390/149‑1 (MQ=11)

                                                                         tCAAAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTAGAGGTAGTAAAGCAGTCAGGTTACGCGGGGTGCCGCAGCCTCGAAGCTCGTCGCGCTCTTAACACGAAGGGCGGCGGTGCACATCCGGCCCCCGc                                                                             >  2:3432928/1‑149 (MQ=14)

                                                                            taccccGAAGCTCGTCGGTTCAAATCCTGCCCCCTCAACCAAATACAATTTGATGAAGTCACGGAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGCTTCAAATCCGGCCCCCGCAAc                                                                          <  2:2069969/146‑1 (MQ=11)

                                                                             aaCCCTAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc                                                                         <  2:2523057/149‑1 (MQ=255)

                                                                             aaCCCTAAGGTCGTCGGTTCAAATCCGGAACCCGCAACCAATTAAAATTTGATGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc                                                                         <  2:681175/149‑1 (MQ=255)

                                                                             aaCCCGAATGTCGTATGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc                                                                         <  2:2523056/149‑1 (MQ=255)

                                                                               cccGAAGGTCTTCGGGTCAAATCCGTCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCaa                                                                       <  2:2088956/149‑1 (MQ=255)

                                                                                ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                      <  2:1781287/149‑1 (MQ=40)

                                                                                ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                      >  2:1416433/1‑149 (MQ=40)

                                                                                 cGAAGGTCGTCTGTTCAAATCCTTCCCCCGCAACCAATTATAATTTGATGAAGTCAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATc                                                                     <  2:4084992/149‑1 (MQ=14)

                                                                                   aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                   <  2:3546160/149‑1 (MQ=255)

                                                                                   aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                   <  2:3543794/149‑1 (MQ=255)

                                                                                    aGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                  <  2:2540097/149‑1 (MQ=255)

                                                                                     ggTCGTCGGTTCAAATCCGGCCCGCGCAACCAATTACAATTTGCTGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                                 <  2:3124668/149‑1 (MQ=255)

                                                                                        cgtcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTCaaagtag                                                              >  2:589683/1‑145 (MQ=255)

                                                                                         gtcgGTTCAAATCCGGCCCCCGCAACCAATTAAACTTTGATGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtga                                                             <  1:3945177/149‑1 (MQ=255)

                                                                                         gtcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtga                                                             >  1:3784302/1‑149 (MQ=255)

                                                                                         gtcgGGTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtga                                                             <  2:3046948/149‑1 (MQ=255)

                                                                                          tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgat                                                            <  1:1711213/149‑1 (MQ=255)

                                                                                             gTTCAAATCCGGCCCCCGCAACCAATTAAAGTTTGATGAAGAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgaggcc                                                         >  1:146702/1‑145 (MQ=255)

                                                                                             gTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATCaa                                                         >  1:146706/1‑149 (MQ=255)

                                                                                             gTTCAAATCCGGCCCCCGCAACCAATTAAAATTAGATGAAGAAAAGCATTAAGTTTACGCGGTGTCCAGAAGCCTGGTAGCTCGTCGGGCTCATAAACCGAAGGTCTTCGGATCAATTCCGGCCACCGCAACCAATGAAAttacaccaa                                                         >  1:146873/1‑142 (MQ=14)

                                                                                               tCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTCAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGt                                                       <  2:65550/149‑1 (MQ=255)

                                                                                                cAAATCCGGCCCCCGCAACCAATTAACATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTa                                                      <  1:2586426/149‑1 (MQ=255)

                                                                                                     ccGTCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGc                                                 <  2:2493849/149‑1 (MQ=255)

                                                                                                      cGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGAAAAAGCa                                                >  2:2033613/1‑149 (MQ=255)

                                                                                                        tcccccGCCACGAATTAAATTTTGCTGAAGACTTGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAGGCAGt                                              <  2:2292606/148‑1 (MQ=17)

                                                                                                        ccccccGCAACCAATTAAACTTTGATGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGGAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAGGCAGt                                              <  2:2292630/148‑1 (MQ=255)

                                                                                                         cccccGAAACCAATTAAAATTTGATGAAGTCAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTa                                             <  1:4040570/149‑1 (MQ=255)

                                                                                                             cGCAACCAAATAAAATTTGATGAAGTTAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGt                                         <  2:3666638/149‑1 (MQ=255)

                                                                                                              gcaaagaaTTACAATGTGATGAAGTATTGCATTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTg                                        <  2:2705744/143‑1 (MQ=17)

                                                                                                              gCAAACAATTAAAATTTGTTGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTg                                        <  2:2705283/149‑1 (MQ=255)

                                                                                                               cAACCAATTAAAATTTGATGAATTATAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGa                                       <  2:41242/149‑1 (MQ=255)

                                                                                                                aagcAAATAAAATTTGATGTAGTTTAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAc                                      <  2:1051372/146‑1 (MQ=18)

                                                                                                                aaCCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAc                                      <  1:2197113/149‑1 (MQ=255)

                                                                                                                 aCCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTAAGGTGAcg                                     >  1:1574125/1‑149 (MQ=255)

                                                                                                                    aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgg                                  >  1:1282160/1‑149 (MQ=255)

                                                                                                                    aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTAGAGGAAGTAAGAGCAGTAAGGTGACGCgg                                  >  2:2532454/1‑149 (MQ=255)

                                                                                                                     aTTAAAATTTGATGAAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCggg                                 >  2:2705261/1‑149 (MQ=255)

                                                                                                                         aaaTTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTCATGAAGAAAAAGCATTACTGTGAAGAGGGGtcg                             >  2:3687121/1‑147 (MQ=17)

                                                                                                                            tttGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTAGATCAAGTAAAAGCAGTACGTTGACGCGGGGGGGagc                          >  1:531791/1‑149 (MQ=255)

                                                                                                                            tttGAGGAACTACAGCAGTACGGTGACGCGGGGAGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGagc                          <  1:1915002/149‑1 (MQ=255)

                                                                                                                            cttGATGAAGTCACGCCGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGagc                          <  1:388082/148‑1 (MQ=255)

                                                                                                                                 tgaAGTATCGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCt                     <  2:676494/149‑1 (MQ=255)

                                                                                                                                 tctcgTACAGCACTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCt                     <  2:817401/145‑1 (MQ=255)

                                                                                                                                       aacgCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGc               <  1:802553/146‑1 (MQ=255)

                                                                                                                                        caGCAGTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCTCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt              <  1:346438/148‑1 (MQ=255)

                                                                                                                                        acgCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCTCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt              <  1:2418800/147‑1 (MQ=255)

                                                                                                                                        aaGCAGTCCGCGGACGCGGGGTGGCGCAGCCTGGTCGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAGTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt              <  2:3155851/149‑1 (MQ=14)

                                                                                                                                        aaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTCAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt              >  1:680124/1‑149 (MQ=255)

                                                                                                                                        aaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt              >  1:3763689/1‑149 (MQ=255)

                                                                                                                                        aaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt              >  1:3763688/1‑149 (MQ=255)

                                                                                                                                         aGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTCGCtc             >  2:3764743/1‑149 (MQ=255)

                                                                                                                                         aGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtc             >  1:2593320/1‑149 (MQ=255)

                                                                                                                                         aGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATATCAGCCAGTAAATTCAGTATGGTGAGGGGGTGTGGAGAAGACTGGTTGCta             >  2:1365244/1‑148 (MQ=14)

                                                                                                                                          gCATTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcg            >  1:415753/1‑149 (MQ=255)

                                                                                                                                              tccGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg        <  2:2584934/147‑1 (MQ=255)

                                                                                                                                                cGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAATTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGc      <  2:1697782/149‑1 (MQ=255)

TGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAT  >  CP009273/2940706‑2941002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`