Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,941,341 G→A 100% L343L (CTC→CTT)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,941,3410GA98.8% 841.1 / ‑0.1 255L343L (CTC→CTT) amiCN‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (135/117);  total (135/120)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 255 total aligned reads displayed.

GGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCA  >  CP009273/2941196‑2941486
                                                                                                                                                 |                                                                                                                                                 
ggAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGc                                                                                                                                                <  2:1254142/149‑1 (MQ=255)
     gTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAtt                                                                                                                                           <  1:71923/149‑1 (MQ=255)
     gTCGCCGTTTTCAGTTTAAGCAATTCCTCAACGTTACTTATAAACGCCGTTTAGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAAACCGGCCAGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTAACAAGCATAtt                                                                                                                                           >  2:741540/1‑149 (MQ=255)
       cGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCa                                                                                                                                         <  1:239816/149‑1 (MQ=255)
          cGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTa                                                                                                                                      >  1:3683230/1‑149 (MQ=255)
           gTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTAc                                                                                                                                     <  1:506436/149‑1 (MQ=255)
           gTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTAc                                                                                                                                     <  1:2573343/149‑1 (MQ=255)
               tCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCt                                                                                                                                 <  1:666684/149‑1 (MQ=255)
                 aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCttt                                                                                                                               >  2:847358/1‑149 (MQ=255)
                 aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCttt                                                                                                                               <  1:2171485/149‑1 (MQ=255)
                   tttACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTAc                                                                                                                             <  1:3524807/149‑1 (MQ=255)
                     tACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCa                                                                                                                           <  2:3466515/149‑1 (MQ=255)
                       cGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCaaa                                                                                                                         <  2:271924/149‑1 (MQ=255)
                        gCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAAc                                                                                                                        >  2:3288887/1‑149 (MQ=255)
                        gCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAAc                                                                                                                        >  2:1238450/1‑149 (MQ=255)
                        gCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAAc                                                                                                                        >  2:2460760/1‑149 (MQ=255)
                         ctctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGACTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACt                                                                                                                       >  2:3817326/1‑149 (MQ=255)
                           ctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTc                                                                                                                     <  1:1000033/149‑1 (MQ=255)
                           ctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTc                                                                                                                     <  2:175977/149‑1 (MQ=255)
                           ctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTc                                                                                                                     >  2:3059263/1‑149 (MQ=255)
                             tCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAg                                                                                                                   >  1:2396911/1‑149 (MQ=255)
                                   aCGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTc                                                                                                             <  1:1098483/149‑1 (MQ=255)
                                   aCGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTc                                                                                                             >  2:3829994/1‑149 (MQ=255)
                                      ttACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGc                                                                                                          >  2:2968542/1‑149 (MQ=255)
                                         cTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAAt                                                                                                       >  1:1304794/1‑149 (MQ=255)
                                           gATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATgg                                                                                                     >  2:3966223/1‑149 (MQ=255)
                                           gATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATgg                                                                                                     <  2:1983859/149‑1 (MQ=255)
                                           gATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATgg                                                                                                     >  2:3215303/1‑149 (MQ=255)
                                           gATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATgg                                                                                                     >  2:432524/1‑149 (MQ=255)
                                           gATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGGTCAACTTGATTTGTAGGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATgg                                                                                                     >  2:2501650/1‑149 (MQ=255)
                                             tAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTc                                                                                                   >  1:1057616/1‑149 (MQ=255)
                                             tAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTc                                                                                                   <  1:3896298/149‑1 (MQ=255)
                                             tAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTc                                                                                                   >  1:44006/1‑149 (MQ=255)
                                               aaCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAg                                                                                                 >  1:1636851/1‑149 (MQ=255)
                                                aCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGc                                                                                                >  2:287373/1‑149 (MQ=255)
                                                aCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGc                                                                                                >  2:2358727/1‑149 (MQ=255)
                                                 cGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCg                                                                                               >  1:2659579/1‑149 (MQ=255)
                                                      tttCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGt                                                                                          >  2:3676685/1‑149 (MQ=255)
                                                        tCGACCAGATTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTTATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTAc                                                                                        <  2:1085628/149‑1 (MQ=255)
                                                          gACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCa                                                                                      >  1:4100353/1‑149 (MQ=255)
                                                              aGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATc                                                                                  >  1:312776/1‑149 (MQ=255)
                                                                 aTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGaa                                                                               >  2:1791132/1‑149 (MQ=255)
                                                                     aGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATg                                                                           >  2:3905257/1‑149 (MQ=255)
                                                                      gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATgg                                                                          >  1:283562/1‑149 (MQ=255)
                                                                      gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATgg                                                                          >  1:2359034/1‑149 (MQ=255)
                                                                      gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATgg                                                                          <  1:1719469/149‑1 (MQ=255)
                                                                      gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATgg                                                                          <  2:2600362/149‑1 (MQ=255)
                                                                      gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATgg                                                                          >  2:2709399/1‑149 (MQ=255)
                                                                       ggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTTATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgt                                                                         <  1:2114533/149‑1 (MQ=255)
                                                                       ggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCCGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgt                                                                         <  1:1741436/149‑1 (MQ=255)
                                                                       ggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgt                                                                         >  1:41663/1‑149 (MQ=255)
                                                                          atatCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGtgtg                                                                      >  2:544014/1‑149 (MQ=255)
                                                                           tatCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTgg                                                                     <  1:2000960/149‑1 (MQ=255)
                                                                            atCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGt                                                                    <  1:860976/149‑1 (MQ=255)
                                                                            atCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGt                                                                    <  1:2295127/149‑1 (MQ=255)
                                                                            atCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGt                                                                    <  1:2212996/149‑1 (MQ=255)
                                                                             tCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTc                                                                   >  1:1450717/1‑149 (MQ=255)
                                                                             tCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTc                                                                   >  1:908367/1‑149 (MQ=255)
                                                                                  tGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACAt                                                              >  1:610998/1‑149 (MQ=255)
                                                                                   gCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATa                                                             <  1:2329144/149‑1 (MQ=255)
                                                                                    ccTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAg                                                            <  1:2955213/149‑1 (MQ=255)
                                                                                     cTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGc                                                           >  1:3391208/1‑149 (MQ=255)
                                                                                      tttAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCg                                                          >  2:1511235/1‑149 (MQ=255)
                                                                                       ttAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGGTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCgg                                                         >  1:12789/1‑149 (MQ=255)
                                                                                       ttAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCgg                                                         >  2:2606992/1‑149 (MQ=255)
                                                                                        tAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGt                                                        <  2:769698/149‑1 (MQ=255)
                                                                                           tACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCAc                                                     <  2:2828116/149‑1 (MQ=255)
                                                                                             cGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCg                                                   >  2:1661418/1‑149 (MQ=255)
                                                                                                cAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCtt                                                <  2:167079/149‑1 (MQ=255)
                                                                                                 aaaCCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCttt                                               <  1:2349038/149‑1 (MQ=255)
                                                                                                 aaaCCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCttt                                               >  2:2155392/1‑149 (MQ=255)
                                                                                                       ggCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCa                                         <  1:1872443/149‑1 (MQ=255)
                                                                                                          cTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGc                                      >  2:2121865/1‑149 (MQ=255)
                                                                                                          cTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGc                                      <  2:1635202/149‑1 (MQ=255)
                                                                                                           tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGcc                                     <  2:3411252/149‑1 (MQ=255)
                                                                                                                  cTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATc                              >  1:2878955/1‑149 (MQ=255)
                                                                                                                  cTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATc                              >  1:3594799/1‑149 (MQ=255)
                                                                                                                   ttGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCa                             >  2:596175/1‑149 (MQ=255)
                                                                                                                    tGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCaa                            >  1:1126653/1‑149 (MQ=255)
                                                                                                                       tttttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTc                         <  2:3641302/149‑1 (MQ=255)
                                                                                                                       tttttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTc                         >  2:4066963/1‑149 (MQ=255)
                                                                                                                       tttttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTc                         >  1:3425179/1‑149 (MQ=255)
                                                                                                                          ttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGa                      <  2:1253836/149‑1 (MQ=255)
                                                                                                                           tATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACGGCTTTACCAAACTTCAGGCTGGCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAg                     <  2:3114783/149‑1 (MQ=255)
                                                                                                                                aGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGtt                >  2:1892986/1‑149 (MQ=255)
                                                                                                                                  cTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCt              <  2:1179631/149‑1 (MQ=255)
                                                                                                                                    tgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTgg            <  1:2681287/149‑1 (MQ=255)
                                                                                                                                     gttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTggg           >  1:185850/1‑149 (MQ=255)
                                                                                                                                      ttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGt          >  2:2021551/1‑149 (MQ=255)
                                                                                                                                      ttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGt          >  2:1068750/1‑149 (MQ=255)
                                                                                                                                      ttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGt          >  1:2927121/1‑149 (MQ=255)
                                                                                                                                      ttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGt          >  1:1608065/1‑149 (MQ=255)
                                                                                                                                      ttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTACGAGGCGTTCTGGGt          >  2:1214889/1‑149 (MQ=255)
                                                                                                                                        gATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGttt        <  2:1739444/149‑1 (MQ=255)
                                                                                                                                        gATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGttt        >  2:1492441/1‑149 (MQ=255)
                                                                                                                                          ttttACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtgt      >  2:2088605/1‑149 (MQ=255)
                                                                                                                                          ttttACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtgt      >  2:1708374/1‑149 (MQ=255)
                                                                                                                                          ttttACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtgt      >  1:101170/1‑149 (MQ=255)
                                                                                                                                             tACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGcc   >  1:1154993/1‑149 (MQ=255)
                                                                                                                                             tACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGAGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGcc   >  2:52804/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCA  >  CP009273/2941196‑2941486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑