Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,989,295 C→A 100% pseudogene (85/261 nt) ygeN ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,989,2950CA99.1% 732.3 / ‑2.0 223pseudogene (85/261 nt)ygeNpseudogene
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (114/107);  total (114/109)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

GGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAAC  >  CP009273/2989149‑2989440
                                                                                                                                                  |                                                                                                                                                 
gtgTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACtatttatt                                                                                                                                                 <  2:4009770/147‑1 (MQ=255)
gggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACtatttatt                                                                                                                                                 <  1:3264068/149‑1 (MQ=255)
 ggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTatttattt                                                                                                                                                >  2:2402272/1‑149 (MQ=255)
  gTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttatttc                                                                                                                                               <  1:3479520/149‑1 (MQ=255)
    ataAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCaa                                                                                                                                             >  1:2166008/1‑149 (MQ=255)
     taAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAAt                                                                                                                                            <  1:53835/149‑1 (MQ=255)
      aaaaTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATc                                                                                                                                           <  1:2746028/149‑1 (MQ=255)
       aaaTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATcc                                                                                                                                          >  1:4100747/1‑149 (MQ=255)
        aaTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCa                                                                                                                                         >  2:1975217/1‑149 (MQ=255)
            aTCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAg                                                                                                                                     <  1:1534831/149‑1 (MQ=255)
             tCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGa                                                                                                                                    >  2:1229049/1‑149 (MQ=255)
               cATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGaaa                                                                                                                                  <  2:356451/149‑1 (MQ=255)
                  ctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAAttt                                                                                                                               <  2:1777792/149‑1 (MQ=255)
                   tcatcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAAtttt                                                                                                                              <  2:1182278/149‑1 (MQ=255)
                     atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTc                                                                                                                            >  1:3032/1‑149 (MQ=255)
                     atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTc                                                                                                                            >  2:84267/1‑149 (MQ=255)
                     atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTc                                                                                                                            >  1:2687/1‑149 (MQ=255)
                     atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCGGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTc                                                                                                                            <  1:3645871/149‑1 (MQ=255)
                      tcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCt                                                                                                                           <  1:2855737/149‑1 (MQ=255)
                           aatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTaa                                                                                                                      >  2:1621895/1‑149 (MQ=255)
                            atGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAAt                                                                                                                     <  1:1462682/149‑1 (MQ=255)
                             tGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATc                                                                                                                    >  1:1083971/1‑149 (MQ=255)
                             tGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATc                                                                                                                    <  2:2690847/149‑1 (MQ=255)
                               ccTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcac                                                                                                                  <  2:2743794/149‑1 (MQ=255)
                                cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGATAAACTATTTATTTCATTCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                 >  2:3373721/1‑149 (MQ=255)
                                cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                 >  2:378393/1‑149 (MQ=255)
                                cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                 <  1:3087351/149‑1 (MQ=255)
                                cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                 >  2:707731/1‑149 (MQ=255)
                                cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                                                                                 >  2:2446952/1‑149 (MQ=255)
                                 ttttGTATATCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCacaa                                                                                                                <  2:1367660/149‑1 (MQ=255)
                                   ttttataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCacaaca                                                                                                              <  2:2793913/146‑1 (MQ=255)
                                     gTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACAcc                                                                                                            >  2:3913923/1‑149 (MQ=255)
                                     gTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACAcc                                                                                                            >  2:1872921/1‑149 (MQ=255)
                                      tataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAATGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCa                                                                                                           >  1:3440055/1‑149 (MQ=255)
                                       ataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAt                                                                                                          >  1:264343/1‑149 (MQ=255)
                                        taGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAtt                                                                                                         >  1:3721251/1‑149 (MQ=255)
                                        taGCAATCATAATAAGATTTAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAtt                                                                                                         <  2:3208324/149‑1 (MQ=255)
                                         aGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAttt                                                                                                        <  1:2466433/149‑1 (MQ=255)
                                            catcatAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCa                                                                                                     >  1:2135409/1‑149 (MQ=255)
                                             atcatAATAAACTTTTATAAATTTTTCTTCTCTCTTTTCATCAATACTCTGAATTTTTTCTGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAg                                                                                                    <  2:2894583/149‑1 (MQ=255)
                                                ataataACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgag                                                                                                 >  2:1477949/1‑149 (MQ=255)
                                                 taataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgaga                                                                                                <  1:1368353/149‑1 (MQ=255)
                                                  aataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAt                                                                                               <  2:3993734/149‑1 (MQ=255)
                                                  aataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAt                                                                                               >  1:3968831/1‑149 (MQ=255)
                                                       ccTTTAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTAt                                                                                          <  1:2489251/149‑1 (MQ=255)
                                                        cTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATc                                                                                         <  1:1975217/149‑1 (MQ=255)
                                                         ttGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCa                                                                                        <  1:692955/149‑1 (MQ=255)
                                                           gAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAg                                                                                      >  1:3415554/1‑149 (MQ=255)
                                                              tAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTc                                                                                   <  1:168842/149‑1 (MQ=255)
                                                               aaaTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCa                                                                                  <  1:426915/149‑1 (MQ=255)
                                                                aaTTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAt                                                                                 >  2:1554491/1‑149 (MQ=255)
                                                                    tttCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATctct                                                                             >  2:2601937/1‑149 (MQ=255)
                                                                      tcttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAt                                                                           <  2:443356/149‑1 (MQ=255)
                                                                      tcttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAAATATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAt                                                                           <  2:2009363/149‑1 (MQ=255)
                                                                        ttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttt                                                                         <  2:824685/149‑1 (MQ=255)
                                                                            ctctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAAATTTTCTTTTAATCACAACACCAGTTGCAGGAGATTTTATCAAGCTCATCTCTAtattttt                                                                     >  2:393034/1‑149 (MQ=255)
                                                                             tctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttttttt                                                                    >  1:4003172/1‑149 (MQ=255)
                                                                             tctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttttttt                                                                    >  2:1720104/1‑149 (MQ=255)
                                                                               ttttCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCg                                                                  >  2:771282/1‑149 (MQ=255)
                                                                               ttttCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTAAATCCATCAGAAATATTTCTTATAATAACAAAAACAATAGAAGGATATTTTATAAAGATCATATATAttatttttat                                                                  >  2:2830909/1‑147 (MQ=255)
                                                                                 ttCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCa                                                                >  2:3311300/1‑149 (MQ=255)
                                                                                 ttCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCa                                                                <  2:3680887/149‑1 (MQ=255)
                                                                                  tCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCAt                                                               >  1:875801/1‑149 (MQ=255)
                                                                                    cTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATaa                                                             >  2:4079733/1‑149 (MQ=255)
                                                                                       aaTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAAt                                                          <  2:3721185/149‑1 (MQ=255)
                                                                                         tACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGt                                                        >  2:1249502/1‑149 (MQ=255)
                                                                                            tctGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAAt                                                     <  2:3440100/149‑1 (MQ=255)
                                                                                             ctGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAAtt                                                    <  2:1801083/149‑1 (MQ=255)
                                                                                              tGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtc                                                   <  2:1083971/149‑1 (MQ=255)
                                                                                                   ttttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTctctct                                              <  1:618873/149‑1 (MQ=255)
                                                                                                   ttttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTctctct                                              <  1:3675476/149‑1 (MQ=255)
                                                                                                    tttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTa                                             <  1:1846227/149‑1 (MQ=255)
                                                                                                    tttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTa                                             <  1:383591/149‑1 (MQ=255)
                                                                                                           tggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtgt                                      <  2:4100747/149‑1 (MQ=255)
                                                                                                             gtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTg                                    <  1:806595/149‑1 (MQ=255)
                                                                                                                   agaagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCaa                              >  1:3064884/1‑149 (MQ=255)
                                                                                                                      agGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTg                           >  1:3322397/1‑149 (MQ=255)
                                                                                                                       ggATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTgg                          >  1:1518977/1‑149 (MQ=255)
                                                                                                                          ttttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                       >  1:1312294/1‑149 (MQ=255)
                                                                                                                          ttttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                       <  2:2465972/149‑1 (MQ=255)
                                                                                                                          ttttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                       >  1:839969/1‑149 (MQ=255)
                                                                                                                           tttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAAc                      <  1:994493/149‑1 (MQ=255)
                                                                                                                             tttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACgg                    <  2:1526158/149‑1 (MQ=255)
                                                                                                                                cTTTAAGAGAAACTATTTATTTCAATCCATCATAATTTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGAATAAAATTTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGa                 <  1:2850667/149‑1 (MQ=255)
                                                                                                                                 tttAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGAt                <  1:1229049/149‑1 (MQ=255)
                                                                                                                                 tttAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGAt                <  1:1759045/149‑1 (MQ=255)
                                                                                                                                    aaGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt             >  1:513003/1‑149 (MQ=255)
                                                                                                                                     agatAAACTTTTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAAAACCATTTGCAGGATATTTTATCAATCACATCTATATTTTTTTTCTCTTAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTa            <  1:2402326/149‑1 (MQ=255)
                                                                                                                                     agagAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTa            >  1:3751628/1‑149 (MQ=255)
                                                                                                                                     agagAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTa            <  1:2402227/149‑1 (MQ=255)
                                                                                                                                     agagAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTa            >  2:3775718/1‑149 (MQ=255)
                                                                                                                                     agagAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTa            >  2:2471016/1‑149 (MQ=255)
                                                                                                                                      gagaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAt           <  1:2785803/149‑1 (MQ=255)
                                                                                                                                        gaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt         <  2:254973/149‑1 (MQ=255)
                                                                                                                                        gaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt         >  2:1422051/1‑149 (MQ=255)
                                                                                                                                        gaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt         <  1:125438/149‑1 (MQ=255)
                                                                                                                                          aaCTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTgg       <  1:405550/149‑1 (MQ=255)
                                                                                                                                           aCTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGt      <  1:1907480/149‑1 (MQ=255)
                                                                                                                                               tttatttcAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTcaac  <  2:3968900/149‑1 (MQ=255)
                                                                                                                                               tttatttcAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTcaac  >  2:2154951/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAAC  >  CP009273/2989149‑2989440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑