Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,999,827 C→A 100% D69E (GAC→GAA)  ygeW → putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,999,8270CA99.5% 728.4 / ‑4.1 220D69E (GAC→GAA) ygeWputative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (124/95);  total (125/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 222 total aligned reads displayed.

GAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAC  >  CP009273/2999681‑2999971
                                                                                                                                                  |                                                                                                                                                
gagaAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGaaaa                                                                                                                                                <  1:3822701/149‑1 (MQ=255)
   aaaGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCAGCGAAAACTc                                                                                                                                             >  2:2626171/1‑149 (MQ=255)
    aaGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTcc                                                                                                                                            <  1:1531150/149‑1 (MQ=255)
     ataTTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCa                                                                                                                                           >  2:1059367/3‑149 (MQ=255)
     aGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCa                                                                                                                                           >  1:492879/1‑149 (MQ=255)
        ttttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAccc                                                                                                                                        >  1:2237573/1‑149 (MQ=255)
           tttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTa                                                                                                                                     <  2:1335940/149‑1 (MQ=255)
             tGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTAcc                                                                                                                                   >  2:1911649/1‑149 (MQ=255)
              gTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTAccc                                                                                                                                  <  2:1730643/149‑1 (MQ=255)
               ttAACGGGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCACCGTCGTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCg                                                                                                                                 >  2:2998613/1‑149 (MQ=255)
                  aCGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCtt                                                                                                                              <  2:1116319/149‑1 (MQ=255)
                   cGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTtc                                                                                                                             <  2:2139082/149‑1 (MQ=255)
                        gAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTa                                                                                                                        <  2:720944/149‑1 (MQ=255)
                                  cAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTACTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCcgcg                                                                                                              >  2:3764290/1‑149 (MQ=255)
                                    gATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCt                                                                                                            <  1:3455941/149‑1 (MQ=255)
                                    gATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCt                                                                                                            <  1:2706080/149‑1 (MQ=255)
                                     aTGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCtt                                                                                                           <  2:3567297/149‑1 (MQ=255)
                                      tGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTa                                                                                                          >  1:3706067/1‑149 (MQ=255)
                                      tGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTa                                                                                                          >  2:4064054/1‑149 (MQ=255)
                                       gAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTaa                                                                                                         >  1:1163780/1‑149 (MQ=255)
                                         aCTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAcc                                                                                                       >  1:2849816/1‑149 (MQ=255)
                                            gAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGc                                                                                                    <  1:647278/149‑1 (MQ=255)
                                              aaCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTc                                                                                                  <  2:308867/149‑1 (MQ=255)
                                              aaCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTc                                                                                                  <  1:284290/149‑1 (MQ=255)
                                                 aaGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGc                                                                                               >  2:1010950/1‑149 (MQ=255)
                                                  aGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGcc                                                                                              <  1:2246205/149‑1 (MQ=255)
                                                   gTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCt                                                                                             <  1:3979249/149‑1 (MQ=255)
                                                       tGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGca                                                                                         >  2:677027/1‑149 (MQ=255)
                                                          aCGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCacaa                                                                                      >  2:2242564/1‑149 (MQ=255)
                                                               gCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGa                                                                                 <  1:1049558/149‑1 (MQ=255)
                                                                  gcagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAACGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              <  2:2325398/149‑1 (MQ=255)
                                                                  gcagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              >  1:2956198/1‑149 (MQ=255)
                                                                  gcagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              <  2:220188/149‑1 (MQ=255)
                                                                   cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtctc                                                                             <  2:1822156/149‑1 (MQ=255)
                                                                   cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtctc                                                                             <  1:2193597/149‑1 (MQ=255)
                                                                   cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtctc                                                                             <  2:3498160/149‑1 (MQ=255)
                                                                        ttAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcgacga                                                                        >  2:1099585/1‑149 (MQ=255)
                                                                        ttAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcgacga                                                                        >  2:3185147/1‑149 (MQ=255)
                                                                        ttAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcgacga                                                                        >  2:3933353/1‑149 (MQ=255)
                                                                           aaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAgg                                                                     >  1:94174/1‑149 (MQ=255)
                                                                           aaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAgg                                                                     >  2:718031/1‑149 (MQ=255)
                                                                            aaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGc                                                                    >  2:2456588/1‑149 (MQ=255)
                                                                             aGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCa                                                                   >  2:3593972/1‑149 (MQ=255)
                                                                              gCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCaa                                                                  >  1:823037/1‑149 (MQ=255)
                                                                                aCTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAt                                                                >  2:914907/1‑149 (MQ=255)
                                                                                aCTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAt                                                                >  1:3983120/1‑149 (MQ=255)
                                                                                aCTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAt                                                                <  1:1111272/149‑1 (MQ=255)
                                                                                  tGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATca                                                              >  1:1437407/1‑149 (MQ=255)
                                                                                      tGCTGAAAACATCTCAACCACAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaaa                                                          <  2:3705288/149‑1 (MQ=255)
                                                                                      tGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaaa                                                          >  2:833770/1‑149 (MQ=255)
                                                                                      tGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaaa                                                          >  1:2791640/1‑149 (MQ=255)
                                                                                       gCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAAt                                                         <  1:1061120/149‑1 (MQ=255)
                                                                                       gCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAAt                                                         >  2:1225382/1‑149 (MQ=255)
                                                                                       gCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAAt                                                         >  2:1883897/1‑149 (MQ=255)
                                                                                        cTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATc                                                        <  2:1534110/149‑1 (MQ=255)
                                                                                          gAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGc                                                      >  1:2731498/1‑149 (MQ=255)
                                                                                             aaCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCa                                                   >  1:2758785/1‑149 (MQ=255)
                                                                                               cATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACg                                                 >  1:1247564/1‑149 (MQ=255)
                                                                                                  ctcAACCAAAGTCTTTAATAGTGGATTGGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCg                                              >  2:1353223/1‑149 (MQ=255)
                                                                                                    cAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGaa                                            <  2:654274/149‑1 (MQ=255)
                                                                                                     aaCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGaaa                                           <  2:122275/149‑1 (MQ=255)
                                                                                                      aCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAc                                          <  1:2093762/149‑1 (MQ=255)
                                                                                                        cAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcg                                        >  2:1748931/1‑149 (MQ=255)
                                                                                                        cAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcg                                        >  1:3699612/1‑149 (MQ=255)
                                                                                                          aaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtg                                      >  1:1427601/1‑149 (MQ=255)
                                                                                                          aaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtg                                      >  2:728369/1‑149 (MQ=255)
                                                                                                           aGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtgc                                     >  1:1641198/1‑149 (MQ=255)
                                                                                                            gTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtgcg                                    >  2:2667465/1‑149 (MQ=255)
                                                                                                             tCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtgcgt                                   <  1:2804460/149‑1 (MQ=255)
                                                                                                               tttAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGa                                 >  1:2546872/1‑149 (MQ=255)
                                                                                                               tttAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGa                                 >  2:2212386/1‑149 (MQ=255)
                                                                                                                ttAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGaa                                >  1:115262/1‑149 (MQ=255)
                                                                                                                  aaTAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAAc                              >  1:2063239/1‑149 (MQ=255)
                                                                                                                  aaTAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAAc                              <  1:1774512/149‑1 (MQ=255)
                                                                                                                   aTAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAAcc                             <  2:3050909/149‑1 (MQ=255)
                                                                                                                      gTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGcc                          >  2:2010811/1‑149 (MQ=255)
                                                                                                                        ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCaa                        <  1:3438479/149‑1 (MQ=255)
                                                                                                                           ttAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAatat                     <  2:2650316/149‑1 (MQ=255)
                                                                                                                            tAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATg                    >  1:68847/1‑149 (MQ=255)
                                                                                                                             aGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGa                   >  1:3473963/1‑149 (MQ=255)
                                                                                                                             aGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGa                   <  1:1858831/149‑1 (MQ=255)
                                                                                                                              ggTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAACCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAt                  >  2:2875072/1‑149 (MQ=255)
                                                                                                                                 aTTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtctc               >  2:840268/1‑149 (MQ=255)
                                                                                                                                  tttCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTcc              >  2:3631724/1‑149 (MQ=255)
                                                                                                                                  tttCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTcc              >  1:2361893/1‑149 (MQ=255)
                                                                                                                                    tCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCtt            <  1:2914530/149‑1 (MQ=255)
                                                                                                                                     ccGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTc           <  1:3185147/149‑1 (MQ=255)
                                                                                                                                     ccGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTc           <  2:462954/149‑1 (MQ=255)
                                                                                                                                      cGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCt          <  2:1635880/149‑1 (MQ=255)
                                                                                                                                      cGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCt          <  2:2831706/149‑1 (MQ=255)
                                                                                                                                       gTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg         >  1:1381326/1‑149 (MQ=255)
                                                                                                                                       gTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg         <  2:4104926/149‑1 (MQ=255)
                                                                                                                                       gTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg         <  2:2693102/149‑1 (MQ=255)
                                                                                                                                        tATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgc        <  1:3889986/149‑1 (MQ=255)
                                                                                                                                          ttCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcgc      >  2:888027/1‑149 (MQ=255)
                                                                                                                                             cgcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGa   <  1:2010345/149‑1 (MQ=255)
                                                                                                                                             cgcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGa   >  2:1599285/1‑149 (MQ=255)
                                                                                                                                             cgcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGa   >  2:2975460/1‑149 (MQ=255)
                                                                                                                                             cgcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGa   <  1:4047186/149‑1 (MQ=255)
                                                                                                                                              acgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAc  >  1:3013900/2‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAC  >  CP009273/2999681‑2999971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑