Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,009,187 G→T 100% intergenic (+90/‑232) mocA → / → ygfK CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,009,1870GT96.1% 587.7 / 12.5 193intergenic (+90/‑232)mocA/ygfKCTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (3/5);  new base T (85/100);  total (88/105)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 209 total aligned reads displayed.

TATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTG  >  CP009273/3009043‑3009332
                                                                                                                                                |                                                                                                                                                 
tatTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                               >  2:1444451/1‑149 (MQ=255)
tatTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                               >  1:1088701/1‑149 (MQ=255)
 atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTATTCCTTCCGGCACTCATTATCATAATTATAAGGAATCACTGATTTTGAGAAAAGATaaaaa                                                                                                                                              >  2:2356121/1‑149 (MQ=255)
 atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATaaaaa                                                                                                                                              <  2:3633156/149‑1 (MQ=255)
  ttGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAt                                                                                                                                             <  2:1188873/149‑1 (MQ=255)
   tGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAtt                                                                                                                                            >  1:1288188/1‑149 (MQ=255)
   tGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAtt                                                                                                                                            <  1:377761/149‑1 (MQ=255)
      tACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGAt                                                                                                                                         >  1:1472164/1‑149 (MQ=255)
              acgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGc                                                                                                                                 <  1:2382830/149‑1 (MQ=255)
              acgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGc                                                                                                                                 <  1:172084/149‑1 (MQ=255)
                gacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTg                                                                                                                               <  2:1715959/149‑1 (MQ=255)
                 acTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                              >  2:3972253/1‑149 (MQ=255)
                 acTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                              >  1:896744/1‑149 (MQ=255)
                  cTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGc                                                                                                                             >  2:2683391/1‑149 (MQ=255)
                   tttATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCa                                                                                                                            >  2:1808042/1‑149 (MQ=255)
                   tttATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCa                                                                                                                            >  2:3847219/1‑149 (MQ=255)
                    ttattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaa                                                                                                                           <  1:283362/149‑1 (MQ=255)
                     tattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaat                                                                                                                          >  2:692330/1‑149 (MQ=255)
                      attaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaata                                                                                                                         >  1:3375396/1‑149 (MQ=255)
                      attaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaata                                                                                                                         <  2:1502169/149‑1 (MQ=255)
                       ttaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataa                                                                                                                        <  1:3149031/149‑1 (MQ=255)
                           aGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGAaataataat                                                                                                                    >  1:3550738/1‑149 (MQ=255)
                             cGAAGGAAAGGTATACTGAAATTTAGAAATCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAttt                                                                                                                  <  2:1471788/149‑1 (MQ=255)
                                  gAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAgg                                                                                                             >  1:274503/1‑149 (MQ=255)
                                  gAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAgg                                                                                                             <  1:1165522/149‑1 (MQ=255)
                                  gAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAgg                                                                                                             <  2:3331482/149‑1 (MQ=255)
                                   aaaGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGa                                                                                                            >  2:643419/1‑149 (MQ=255)
                                    aaGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAg                                                                                                           <  2:1526825/149‑1 (MQ=255)
                                       gTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGa                                                                                                        <  1:1352068/149‑1 (MQ=255)
                                        tataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATACTTTGCAGGAGTGAt                                                                                                       >  1:2130855/1‑149 (MQ=255)
                                        tataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAt                                                                                                       >  1:2131008/1‑149 (MQ=255)
                                         ataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATNATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtt                                                                                                      <  1:2084970/149‑1 (MQ=255)
                                           aCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtttt                                                                                                    <  1:2729378/149‑1 (MQ=255)
                                           aCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtttt                                                                                                    >  2:1959373/1‑149 (MQ=255)
                                            cTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTa                                                                                                   >  1:2259898/1‑149 (MQ=255)
                                              gAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAt                                                                                                 <  1:2545029/149‑1 (MQ=255)
                                              gAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAt                                                                                                 <  1:3179376/149‑1 (MQ=255)
                                                aaTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtc                                                                                               <  1:3350194/149‑1 (MQ=255)
                                                 aTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtct                                                                                              >  2:2268541/1‑149 (MQ=255)
                                                 aTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtct                                                                                              <  2:3077524/149‑1 (MQ=255)
                                                 aTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtct                                                                                              <  2:3565535/149‑1 (MQ=255)
                                                       aaaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTa                                                                                        <  2:3919686/149‑1 (MQ=255)
                                                        aaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAg                                                                                       >  1:1210889/1‑149 (MQ=255)
                                                        aaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAg                                                                                       <  1:2231162/149‑1 (MQ=255)
                                                          aCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCa                                                                                     >  1:674822/1‑149 (MQ=255)
                                                            gTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCAcc                                                                                   <  1:550144/149‑1 (MQ=255)
                                                                  aaaCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAg                                                                             <  1:1808042/149‑1 (MQ=255)
                                                                    aCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGtt                                                                           <  2:2776057/149‑1 (MQ=255)
                                                                    aCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGtt                                                                           >  1:2223097/1‑149 (MQ=255)
                                                                       aTTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcat                                                                        <  2:3359368/149‑1 (MQ=255)
                                                                        ttGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcatt                                                                       >  1:3628738/1‑149 (MQ=255)
                                                                             ttCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACaa                                                                  >  1:2912163/1‑149 (MQ=255)
                                                                                   tatTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAGTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGcc                                                            >  2:5093/1‑149 (MQ=255)
                                                                                     tttAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCt                                                          >  1:1151916/1‑149 (MQ=255)
                                                                                      ttAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCtt                                                         <  1:4022526/149‑1 (MQ=255)
                                                                                      ttAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCtt                                                         <  2:2174753/149‑1 (MQ=255)
                                                                                      ttAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCtt                                                         <  1:511480/149‑1 (MQ=255)
                                                                                       tAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTa                                                        <  1:257766/149‑1 (MQ=255)
                                                                                         aTCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAg                                                      <  1:3827970/149‑1 (MQ=255)
                                                                                           ccTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGtt                                                    <  2:885835/149‑1 (MQ=255)
                                                                                           ccTTCCCGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGtt                                                    <  2:2945020/149‑1 (MQ=255)
                                                                                            cTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                   >  2:453132/1‑149 (MQ=255)
                                                                                            cTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                   <  2:2690929/149‑1 (MQ=255)
                                                                                            cTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                   >  2:2521526/1‑149 (MQ=255)
                                                                                             ttCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTc                                                  <  1:2379944/149‑1 (MQ=255)
                                                                                             ttCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTc                                                  <  2:2699784/149‑1 (MQ=255)
                                                                                                  gCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgcc                                             <  1:3166911/149‑1 (MQ=255)
                                                                                                    aCTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgccgc                                           >  1:2352533/1‑149 (MQ=255)
                                                                                                    aCTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgccgc                                           >  2:1081056/1‑149 (MQ=255)
                                                                                                            tCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtt                                   <  2:1888867/149‑1 (MQ=255)
                                                                                                            tCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtt                                   <  2:1889032/149‑1 (MQ=255)
                                                                                                              aTAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtttt                                 <  2:1288188/149‑1 (MQ=255)
                                                                                                              aTAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtttt                                 <  1:582534/149‑1 (MQ=255)
                                                                                                               tAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTATGTTTCGAGCCGCCTCACAttttt                                >  2:70520/1‑149 (MQ=255)
                                                                                                               tAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAttttt                                <  1:801400/149‑1 (MQ=255)
                                                                                                                aaTGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtttttt                               <  1:227501/149‑1 (MQ=255)
                                                                                                                aaTGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtttttt                               <  2:2118237/149‑1 (MQ=255)
                                                                                                                  tGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTta                             >  2:599345/1‑149 (MQ=255)
                                                                                                                   gATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtat                            >  1:3174468/1‑149 (MQ=255)
                                                                                                                     tAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTatat                          <  2:3163756/149‑1 (MQ=255)
                                                                                                                      aaGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAtt                         <  1:3158519/149‑1 (MQ=255)
                                                                                                                         gAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTcc                      <  2:3417180/149‑1 (MQ=255)
                                                                                                                         gAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGACTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTcc                      >  1:623392/1‑149 (MQ=255)
                                                                                                                              aCTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCa                 >  1:3696137/1‑149 (MQ=255)
                                                                                                                                tGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa               <  2:2322710/149‑1 (MQ=255)
                                                                                                                                  aTTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc             <  1:2011931/149‑1 (MQ=255)
                                                                                                                                  aTTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc             <  1:4049121/149‑1 (MQ=255)
                                                                                                                                  aTTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc             >  1:438644/1‑149 (MQ=255)
                                                                                                                                   ttttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCt            >  2:3488336/1‑149 (MQ=255)
                                                                                                                                   ttttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCAGCCAAACCt            >  2:300790/1‑149 (MQ=255)
                                                                                                                                    tttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTg           >  1:3888910/1‑149 (MQ=255)
                                                                                                                                     ttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGATTCGAGCCGCCTCACATTTTTTTATATTTACCGCCAAACCTgg          >  1:685862/1‑149 (MQ=255)
                                                                                                                                       gagaaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACGGGAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCa        >  2:2415564/1‑149 (MQ=255)
                                                                                                                                         gaaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAag      <  1:1377506/149‑1 (MQ=255)
                                                                                                                                          aaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAcga     >  1:755966/1‑149 (MQ=255)
                                                                                                                                          aaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAaga     >  2:1539485/1‑149 (MQ=255)
                                                                                                                                            aagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAgt   <  1:692330/149‑1 (MQ=255)
                                                                                                                                            aagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAgt   <  2:781416/149‑1 (MQ=255)
                                                                                                                                            aagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCAAAACCGGGCAAGAgt   <  2:1749324/149‑1 (MQ=255)
                                                                                                                                             agataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAgtg  >  1:760801/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTG  >  CP009273/3009043‑3009332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑