Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,181 G→T 100% H429N (CAT→AAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,1810GT99.0% 738.8 / ‑1.8 233H429N (CAT→AAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base T (128/101);  total (128/105)
Fisher's exact test for biased strand distribution p-value = 3.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

CATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAAC  >  CP009273/3030035‑3030327
                                                                                                                                                  |                                                                                                                                                  
      ggCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCaa                                                                                                                                            >  2:1858368/1‑149 (MQ=255)
      ggCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCaa                                                                                                                                            >  1:3694795/1‑149 (MQ=255)
         cGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACt                                                                                                                                         <  2:2175233/149‑1 (MQ=255)
           ggCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTc                                                                                                                                       >  1:1891312/1‑149 (MQ=255)
              ttAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGc                                                                                                                                    <  2:1747123/149‑1 (MQ=255)
               tAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGca                                                                                                                                   >  1:3470164/1‑149 (MQ=255)
               tAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGca                                                                                                                                   <  1:3209067/149‑1 (MQ=255)
                 aCGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGcatc                                                                                                                                 >  1:1719183/1‑149 (MQ=255)
                 aCGGACCGTCTGATACCACGTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGcatc                                                                                                                                 <  2:2164616/149‑1 (MQ=255)
                       cGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCa                                                                                                                           <  2:2753148/149‑1 (MQ=255)
                        gTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAg                                                                                                                          >  2:3627860/1‑149 (MQ=255)
                        gTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAg                                                                                                                          <  1:2795087/149‑1 (MQ=255)
                         tCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGc                                                                                                                             <  1:1701027/145‑1 (MQ=255)
                           tGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGt                                                                                                                       >  2:751632/1‑149 (MQ=255)
                            gATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTa                                                                                                                      <  1:2654608/149‑1 (MQ=255)
                            gATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTa                                                                                                                      >  1:231049/1‑149 (MQ=255)
                            gATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTa                                                                                                                      >  1:3544745/1‑149 (MQ=255)
                             aTACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTag                                                                                                                     <  2:3541542/149‑1 (MQ=255)
                              tACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTaga                                                                                                                    >  1:2116787/1‑149 (MQ=255)
                                ccACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgt                                                                                                                  >  1:3382788/1‑149 (MQ=255)
                                    ttCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgtgt                                                                                                              >  2:952788/1‑149 (MQ=255)
                                    ttCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgtgt                                                                                                              <  2:1646570/149‑1 (MQ=255)
                                     tCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTc                                                                                                             <  1:567729/149‑1 (MQ=255)
                                                tAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCa                                                                                                  <  2:552336/149‑1 (MQ=255)
                                                  gCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAgc                                                                                                >  1:1586203/1‑149 (MQ=255)
                                                     aaGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCa                                                                                             >  2:1570950/1‑149 (MQ=255)
                                                      aGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCAt                                                                                            >  1:1906699/1‑149 (MQ=255)
                                                       gggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTAGCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATc                                                                                           <  1:1742857/149‑1 (MQ=255)
                                                       gggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATc                                                                                           <  1:1876554/149‑1 (MQ=255)
                                                         gTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCAc                                                                                         <  2:905027/149‑1 (MQ=255)
                                                            cAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCa                                                                                      <  2:3710098/149‑1 (MQ=255)
                                                             aGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCat                                                                                     <  2:2815561/149‑1 (MQ=255)
                                                              gCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                    <  1:1740983/149‑1 (MQ=255)
                                                              gCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                    <  1:2026063/149‑1 (MQ=255)
                                                               ccACTCAGTAACCAGTTCGACAAAACTTTGTTGAAAGAGTTTGAATTTATCCTCTTCCCGCGACAAACCCGCCGCCATCGCATTACAGCCAAACTTCAGCATCATGCCAGGTTCGAGTGTGTCTAATCGCTCCAGCTCATCACGCatat                                                                                   >  2:1236688/1‑149 (MQ=255)
                                                                     aGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGcc                                                                             >  2:490420/1‑149 (MQ=255)
                                                                     aGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGcc                                                                             >  1:3376650/1‑149 (MQ=255)
                                                                      gTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGccc                                                                            <  1:2593485/149‑1 (MQ=255)
                                                                      gTAACCAGTTCGCCAAACCGTTGTTGAAAGAGGTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGccc                                                                            >  2:60492/1‑149 (MQ=255)
                                                                        aaCCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCt                                                                          <  1:3698439/149‑1 (MQ=255)
                                                                         aCCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTg                                                                         >  1:1063922/1‑149 (MQ=255)
                                                                            aGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAAt                                                                      >  2:1201487/1‑149 (MQ=255)
                                                                              ttCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATgg                                                                    >  1:1625801/1‑149 (MQ=255)
                                                                                   cAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGa                                                               <  1:1255564/149‑1 (MQ=255)
                                                                                   cAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGa                                                               <  2:2291423/149‑1 (MQ=255)
                                                                                        cGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGGATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGa                                                          >  1:2422608/1‑149 (MQ=255)
                                                                                        cGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGa                                                          >  2:2726560/1‑149 (MQ=255)
                                                                                        cGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGa                                                          >  1:2426634/1‑149 (MQ=255)
                                                                                          ttgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAc                                                        <  2:3678333/149‑1 (MQ=255)
                                                                                             tttaaaGAGTTTTAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGATTTACCGCCAAACTTCAGCATCATGCCTGGGTAGTGTGTGTATAATCGCTCCAGCGAATCACGAATATGCAGCCCCTGAATGGAGCGACCGGAACCtt                                                     <  1:3925908/146‑1 (MQ=255)
                                                                                             ttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCtt                                                     <  2:2896952/149‑1 (MQ=255)
                                                                                             ttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCtt                                                     <  2:788208/149‑1 (MQ=255)
                                                                                             ttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCAGGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCtt                                                     <  1:3925993/149‑1 (MQ=255)
                                                                                              tgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCttt                                                    >  1:495627/1‑149 (MQ=255)
                                                                                              tgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCttt                                                    <  2:3382738/149‑1 (MQ=255)
                                                                                              tgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCttt                                                    <  2:3382959/149‑1 (MQ=255)
                                                                                              tgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCttt                                                    >  1:2564824/1‑149 (MQ=255)
                                                                                                 aaGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAg                                                 >  2:1122553/1‑149 (MQ=255)
                                                                                                    agTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGt                                              <  2:3076439/149‑1 (MQ=255)
                                                                                                      tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCTCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTAc                                            <  2:2359402/149‑1 (MQ=255)
                                                                                                      tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTAc                                            >  1:1763814/1‑149 (MQ=255)
                                                                                                      tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTAc                                            <  2:404492/149‑1 (MQ=255)
                                                                                                          aaTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTc                                        <  1:590829/149‑1 (MQ=255)
                                                                                                           aTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                       >  2:3898647/1‑149 (MQ=255)
                                                                                                           aTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                       >  2:3788125/1‑149 (MQ=255)
                                                                                                           aTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                       >  2:3118795/1‑149 (MQ=255)
                                                                                                            tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                      >  2:1430137/1‑149 (MQ=255)
                                                                                                            tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                      <  2:3242897/149‑1 (MQ=255)
                                                                                                            tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                      >  2:1357364/1‑149 (MQ=255)
                                                                                                            tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                      >  2:1431137/1‑149 (MQ=255)
                                                                                                               aTCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctg                                   >  1:3403910/1‑149 (MQ=255)
                                                                                                                tCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                                  <  2:3880979/149‑1 (MQ=255)
                                                                                                                tCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                                  >  1:960378/1‑149 (MQ=255)
                                                                                                                tCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                                  <  2:87974/149‑1 (MQ=255)
                                                                                                                  ctctTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgctg                                >  1:1475492/1‑149 (MQ=255)
                                                                                                                  ctctTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgctg                                <  1:3627860/149‑1 (MQ=255)
                                                                                                                    ctTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGgc                              <  1:3118795/149‑1 (MQ=255)
                                                                                                                     ttCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGACCCTTTCAGCGTACCGTCACCTGCTGgcg                             >  1:779398/1‑149 (MQ=255)
                                                                                                                        cAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaa                          <  2:1151216/149‑1 (MQ=255)
                                                                                                                         aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaaa                         >  1:1014424/1‑149 (MQ=255)
                                                                                                                         aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaaa                         <  2:686296/149‑1 (MQ=255)
                                                                                                                         aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaaa                         >  1:3070939/1‑149 (MQ=255)
                                                                                                                         aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaaa                         >  1:1585884/1‑149 (MQ=255)
                                                                                                                         aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaaa                         >  1:955544/1‑149 (MQ=255)
                                                                                                                          gCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAc                        >  1:3562064/1‑149 (MQ=255)
                                                                                                                             aCAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                     >  2:1070422/1‑149 (MQ=255)
                                                                                                                             aCAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                     >  2:317988/1‑149 (MQ=255)
                                                                                                                             aCAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                     >  2:2187165/1‑149 (MQ=255)
                                                                                                                             aCAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCAAATCGCTACAGCGCATCACGAATATGCAGCCCCAGAATGGAGCGACCGGAACCTATCAGCGTACCGTCACCTGCTGGCGCAAActcg                     >  2:3193394/1‑149 (MQ=255)
                                                                                                                              cAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGa                    >  1:852392/1‑149 (MQ=255)
                                                                                                                                 aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa                 >  2:3796689/1‑149 (MQ=255)
                                                                                                                                 aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa                 <  2:1802642/149‑1 (MQ=255)
                                                                                                                                 aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa                 >  2:3974408/1‑149 (MQ=255)
                                                                                                                                 aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa                 <  1:3728080/149‑1 (MQ=255)
                                                                                                                                 aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa                 <  2:1155981/149‑1 (MQ=255)
                                                                                                                                 aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa                 >  1:1050918/1‑149 (MQ=255)
                                                                                                                                      ccgccATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGa            <  1:3522279/149‑1 (MQ=255)
                                                                                                                                         ccATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACgg         >  2:1838618/1‑149 (MQ=255)
                                                                                                                                              gCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa    >  1:2885052/1‑149 (MQ=255)
                                                                                                                                              gCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa    <  2:231049/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAAC  >  CP009273/3030035‑3030327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑