Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,049,740 C→A 100% L47L (CTC→CTA)  fau → 5‑formyltetrahydrofolate cyclo‑ligase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,049,7400CA98.2% 740.5 / 2.9 243L47L (CTC→CTA) fau5‑formyltetrahydrofolate cyclo‑ligase family protein
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (111/127);  total (112/131)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

CGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATG  >  CP009273/3049594‑3049885
                                                                                                                                                  |                                                                                                                                                 
cGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGa                                                                                                                                                 <  1:1568522/149‑1 (MQ=255)
 gCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGac                                                                                                                                                <  2:1231392/149‑1 (MQ=255)
  cAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTTTTGGCGAACTAGaca                                                                                                                                               >  1:3602066/1‑149 (MQ=255)
  cAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGaca                                                                                                                                               <  2:1365190/149‑1 (MQ=255)
    aaaTGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACGTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGTTGGCGAACTAGACAcc                                                                                                                                             >  1:3765354/1‑149 (MQ=255)
     aaTGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTGCTCTCTTTTTATTGCGAACTATACAccc                                                                                                                                            >  1:645686/1‑149 (MQ=255)
           tCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTTTTGCTCTCTTTTGATGGCGAAATAGAAACccacccac                                                                                                                                      >  1:3166000/1‑149 (MQ=255)
            cGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACt                                                                                                                                     <  1:331134/149‑1 (MQ=255)
              gCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCa                                                                                                                                   >  2:1565320/1‑149 (MQ=255)
                 aCGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAg                                                                                                                                >  2:1305332/1‑149 (MQ=255)
                       tcgtGCGTTAACGCCGGAACAACAGCAGGAAATGTGTAAACAATCCGATACCCGGATGATGACTTATCCCCCGGTGGTGATGTCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCTAACTAGACACCCAGCCACTCATAGaacaac                                                                                                                          <  2:879873/149‑1 (MQ=255)
                         gtGCTTTAACGCCGGAACAACAGCAGGAAATGGGTCAACACGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTTTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAActc                                                                                                                        <  1:1780038/149‑1 (MQ=255)
                         gtGCTTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGTTGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACCCATAGAACAActc                                                                                                                        <  1:107537/149‑1 (MQ=255)
                         gtGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAActc                                                                                                                        <  2:3939810/149‑1 (MQ=255)
                         gtGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTCTTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCCCTCATAGAACAActc                                                                                                                        >  2:892081/1‑149 (MQ=255)
                            cGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTgg                                                                                                                     <  2:3753435/149‑1 (MQ=255)
                              ttAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGgcg                                                                                                                   >  2:2532196/1‑149 (MQ=255)
                                  cGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGcc                                                                                                               <  2:621544/149‑1 (MQ=255)
                                  cGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTAGTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGcc                                                                                                               <  2:2244316/149‑1 (MQ=255)
                                      ggAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTa                                                                                                           <  1:3224673/149‑1 (MQ=255)
                                      ggAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTATTACTCTCGTTTGATGGCGAACTAGACACCCACCCCCACAAAGAACAACACTGGCGCGCCggga                                                                                                           >  1:3651913/1‑147 (MQ=255)
                                       gAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTATTGCTCTCTTTTGTTGGCGAACTAGACACCCCCCCCCACATAGGACAAAAAGGGCGCGCCGGtga                                                                                                          >  1:2115446/1‑147 (MQ=255)
                                        aacaacAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTATACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAg                                                                                                         >  2:3830236/1‑149 (MQ=255)
                                               gcagGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCATTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACACTGGCGCGCCGGTAAGCGCGtat                                                                                                  >  2:3230179/1‑149 (MQ=255)
                                               gcagGAAATGGGTCAAAAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTAATCCAATCATTTGATTGCGAAAAAGACAACAACCCACACAAATAAAAACTAATGCGCGACGGGAAGCGCCtaa                                                                                                  >  2:159425/1‑148 (MQ=255)
                                                 agGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTatat                                                                                                <  1:343102/149‑1 (MQ=255)
                                                  ggAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTGTTGCGGTCTTTTTTTTGGGGACTAGAAACCCACCCCCACAAATTAAAACAAATGCGCGCCCGGAAGCGCGGatata                                                                                               >  1:1423409/1‑148 (MQ=255)
                                                     aaTGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTc                                                                                            <  2:349928/149‑1 (MQ=255)
                                                     aaTGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTACTCTATTTTTTTTTCGAACAATACACCCAGCCCCACCAAGAAAAAAACTGGCGCGCCGGTAAGCGCGTATATCTTc                                                                                            >  1:3588369/1‑149 (MQ=255)
                                                      aTGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTATTACTCTCTTTTTATGGCGAAATAGAAACCCACCCACACCTAGAACAACACTGGCGCGCCCGTAACCGCGGATATCTTcc                                                                                           >  1:2604040/1‑149 (MQ=255)
                                                         ggTCAACACGCCGCTACCCGGATGATGACTTATCCCCCGGGGGTGATGGCACATACGGTCGCTTTAGGCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATATAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCact                                                                                        <  1:108065/149‑3 (MQ=255)
                                                         ggTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGt                                                                                        <  2:477396/149‑1 (MQ=255)
                                                           tCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGttt                                                                                      <  2:2983680/149‑1 (MQ=255)
                                                           tCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACGAGACACCCAGCCCCTCATAGAACCACTCGGGCGCGCCGGTAAGCGCGTATATCGTCCAGgtt                                                                                      >  2:2412862/1‑149 (MQ=255)
                                                           tCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTGTTGCTCTGTTTTTTTTGCGGACAAGACACCCAGCCACACATAGAACAAATCTGGCGCGCCGGGAAGCGCGGATATCTTACAGgtt                                                                                      >  1:241777/1‑149 (MQ=255)
                                                             aacaaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTg                                                                                    <  1:2926254/149‑1 (MQ=255)
                                                             aacaaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTg                                                                                    >  2:271072/1‑149 (MQ=255)
                                                              acaaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTTTTCCTCTCTTTTGATGGCGAACTAGACACCCACCCACTCATAGAAAAACACTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGc                                                                                   >  1:3068889/1‑149 (MQ=255)
                                                              acaaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTATACACCCAGCCACTCATAGAACAACACTGGCGCGCCGGTAAGCGCGTATATCTTACAGTTTTGc                                                                                   >  1:3078152/1‑149 (MQ=255)
                                                              acaaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGc                                                                                   <  2:2129251/149‑1 (MQ=255)
                                                              acaaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGc                                                                                   <  2:3086647/149‑1 (MQ=255)
                                                                aaGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTATTGCTCTCTTTTTATTGCGAACTAGACACCCCGCCACTCCAATAACAACACTGGCGCGCCGGGAAGCGCGGATATATTCCAGTTTTGCAt                                                                                 >  1:2302097/1‑149 (MQ=255)
                                                                 agacGCTACCCGGATGATGACTTATCCCCCTGTGGTGATGGCACATACGGTCGCTGGATTGATCTCTTTTTATTGCGAAATAGACAACCAGCCAATAAAACAACAAAAAAGGAGCGCCCGTAAGCGCGGATATATTCCACTTTTgacta                                                                                >  2:1725675/4‑145 (MQ=255)
                                                                      cTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCttt                                                                           <  1:3911746/149‑1 (MQ=255)
                                                                           cGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTATTGCTCTCGTTTGATGGGGAACTAGACACCCACCCACACAAATAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGGTTTGCATCCCGTTtgtgg                                                                      >  1:2384365/1‑148 (MQ=255)
                                                                            ggATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATATTGCAGTTTTTCATCCCGTTAGTGcc                                                                     >  2:779097/1‑149 (MQ=255)
                                                                              atgatgACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCgg                                                                   <  2:36634/149‑1 (MQ=255)
                                                                                gatgaCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTa                                                                 <  1:2888690/149‑1 (MQ=255)
                                                                                  tgaCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAt                                                               <  2:3166000/149‑1 (MQ=255)
                                                                                   gaCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAtt                                                              <  2:3557772/149‑1 (MQ=255)
                                                                                   gaCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAtt                                                              <  2:1016700/149‑1 (MQ=255)
                                                                                   gaCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAtt                                                              <  1:2334612/149‑1 (MQ=255)
                                                                                    aCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAttt                                                             <  1:923597/149‑1 (MQ=255)
                                                                                    aCTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGCAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAttt                                                             <  2:1801679/149‑1 (MQ=255)
                                                                                    aCTTATCCCCCGGTGGTGATGGCACATACGGTCGCGGTGTTCCGCTCTTTTGTTGGCGAACGAGACACCCAGCCACGCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAAttt                                                             >  2:3170584/1‑149 (MQ=255)
                                                                                     cTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTTATTGCGAACAAGACACCCAGCCCCACATAGAACAACTCTGGCGCGCCGGTAAGCGCGGATATCTTACAGTTTTGCATCCCGTTAGTGGCGGTAAattt                                                            >  2:1099131/1‑148 (MQ=255)
                                                                                      ttATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGc                                                           <  2:1389290/149‑1 (MQ=255)
                                                                                      ttATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGc                                                           <  2:3275912/149‑1 (MQ=255)
                                                                                      ttATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTATACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGc                                                           >  2:1696025/1‑149 (MQ=255)
                                                                                      ttATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCATCTCTTTGGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGc                                                           <  2:1648707/149‑1 (MQ=255)
                                                                                        aTCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTGTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTTCATCCCTTTAGTGCCGGTAATTTGCTg                                                         <  2:1585476/149‑1 (MQ=255)
                                                                                         tCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGt                                                        <  2:2604040/149‑1 (MQ=255)
                                                                                         tCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGt                                                        <  2:2351642/149‑1 (MQ=255)
                                                                                          cccccGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGtt                                                       <  1:1565320/149‑1 (MQ=255)
                                                                                           ccccGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATATTCCAGTTTTTCATCCCTTTAGTGCCGGTAATTTGCTGTTc                                                      >  2:601859/1‑149 (MQ=255)
                                                                                           ccccGGTGGTGATGGCACATACGGTCGCGGTATTCCTGTATTTTGATTGCGCACTAGAAACCCAGCCACTCATATAAAAAATCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCGTTAGTGCCGGTAATTTTCTGttg                                                      >  2:1557345/1‑148 (MQ=255)
                                                                                            cccGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTcc                                                     <  2:3730751/149‑1 (MQ=255)
                                                                                             ccGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCt                                                    <  1:1152932/149‑1 (MQ=255)
                                                                                                 tggtgATGGCACATACGGTCGCTGTATTGCTCTCTTTTGATTGCGAACTAGACACCCAGCCACTCATAGAACAAATCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTACTGAAt                                                >  2:1185907/1‑149 (MQ=255)
                                                                                                    tgATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTGAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTAc                                             <  1:825638/149‑1 (MQ=255)
                                                                                                     gATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTAcc                                            <  1:1196359/149‑1 (MQ=255)
                                                                                                       tGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCAt                                          <  2:484330/149‑1 (MQ=255)
                                                                                                        ggCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATc                                         <  1:3735213/149‑1 (MQ=255)
                                                                                                               ccGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAg                                  <  2:2436071/148‑1 (MQ=255)
                                                                                                                    cGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAAc                             <  1:1047109/149‑1 (MQ=255)
                                                                                                                    cGCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAAc                             >  2:1787706/1‑149 (MQ=255)
                                                                                                                     gCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACt                            <  2:2737494/149‑1 (MQ=255)
                                                                                                                     gCTGTATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACt                            <  2:3225574/149‑1 (MQ=255)
                                                                                                                         tATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGtg                        <  2:3298158/149‑1 (MQ=255)
                                                                                                                         tATTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGtg                        >  2:2181668/1‑149 (MQ=255)
                                                                                                                          aTTCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGtga                       <  1:279817/149‑1 (MQ=255)
                                                                                                                           ttCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGtgat                      >  2:4017997/1‑149 (MQ=255)
                                                                                                                           ttCCTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGtgat                      <  1:1233995/149‑1 (MQ=255)
                                                                                                                             ccTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGtgatga                    >  2:3015508/1‑149 (MQ=255)
                                                                                                                             ccTCTCTTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGtgatga                    >  2:1133025/1‑149 (MQ=255)
                                                                                                                              ctctctTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGaa                   <  1:2521393/149‑1 (MQ=255)
                                                                                                                              ctctctTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGaa                   >  2:1353346/1‑149 (MQ=255)
                                                                                                                              ctctctTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGaa                   <  1:890313/149‑1 (MQ=255)
                                                                                                                              ctctctTTTGATGGCGAACGAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGaa                   <  2:1274026/149‑1 (MQ=255)
                                                                                                                               tctctTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAAc                  <  1:171595/149‑1 (MQ=255)
                                                                                                                                ctctTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACa                 >  1:425860/1‑149 (MQ=255)
                                                                                                                                  ctTTTGATGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGAATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAgg               >  2:1862378/1‑149 (MQ=255)
                                                                                                                                         tGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGa        >  1:151012/1‑149 (MQ=255)
                                                                                                                                         tGGCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGa        <  2:2292427/149‑1 (MQ=255)
                                                                                                                                          ggCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGAt       <  1:3818282/149‑1 (MQ=255)
                                                                                                                                          ggCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGAt       >  1:2732651/1‑149 (MQ=255)
                                                                                                                                           gCGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATc      <  1:1142523/149‑1 (MQ=255)
                                                                                                                                            cGAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATcc     <  2:885514/149‑1 (MQ=255)
                                                                                                                                             gAACTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCa    >  1:1246362/1‑149 (MQ=255)
                                                                                                                                              aaCTAGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCAt   >  1:2486122/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATG  >  CP009273/3049594‑3049885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑