Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,213 T→A 100% A753A (GCA→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,2130TA97.3% 732.1 / 6.8 223A753A (GCA→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base A (93/124);  total (95/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 223 total aligned reads displayed.

ACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTC  >  CP009273/3110067‑3110357
                                                                                                                                                  |                                                                                                                                                
aCTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGc                                                                                                                                                <  1:652872/149‑1 (MQ=255)
  ttCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCa                                                                                                                                              >  2:153647/1‑149 (MQ=255)
  ttCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCa                                                                                                                                              >  1:3484789/1‑149 (MQ=255)
  ttCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCa                                                                                                                                              <  2:3653205/149‑1 (MQ=255)
   tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCAt                                                                                                                                             <  1:2837035/149‑1 (MQ=255)
   tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCAt                                                                                                                                             <  1:269925/149‑1 (MQ=255)
   tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCAt                                                                                                                                             >  1:3882813/1‑149 (MQ=255)
   tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCAt                                                                                                                                             <  1:771150/149‑1 (MQ=255)
    ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATc                                                                                                                                            <  1:3412665/149‑1 (MQ=255)
    ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATc                                                                                                                                            <  1:1364180/149‑1 (MQ=255)
    ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATc                                                                                                                                            <  1:3561577/149‑1 (MQ=255)
      ccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGa                                                                                                                                          >  1:4004703/1‑149 (MQ=255)
       cTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                         >  1:2333206/1‑149 (MQ=255)
       cTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                         <  1:3530099/149‑1 (MQ=255)
          tCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGa                                                                                                                                      >  1:1291357/1‑149 (MQ=255)
            ttACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGAcc                                                                                                                                    <  2:534334/149‑1 (MQ=255)
             tACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCt                                                                                                                                   >  2:1977439/1‑149 (MQ=255)
              aCTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTg                                                                                                                                  >  2:3906407/1‑149 (MQ=255)
                   cGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCa                                                                                                                             <  1:2181515/149‑1 (MQ=255)
                      tGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATcc                                                                                                                          >  1:1599536/1‑149 (MQ=255)
                                cAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGca                                                                                                                <  2:3612467/149‑1 (MQ=255)
                                 aGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGcac                                                                                                               <  1:3486917/149‑1 (MQ=255)
                                    gcACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGcacaca                                                                                                            >  1:24722/1‑147 (MQ=255)
                                        cATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCa                                                                                                        >  2:2449440/1‑149 (MQ=255)
                                        cATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCa                                                                                                        >  1:3396466/1‑149 (MQ=255)
                                             aCGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcgc                                                                                                   >  2:3145748/1‑149 (MQ=255)
                                               ggCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACgcgc                                                                                                 >  1:1085767/1‑149 (MQ=255)
                                                gCAGGATACCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGct                                                                                                <  2:254834/149‑1 (MQ=255)
                                                 cAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctc                                                                                               <  2:3775647/149‑1 (MQ=255)
                                                  aGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctct                                                                                              <  1:3439883/149‑1 (MQ=255)
                                                   ggATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                             <  1:468016/149‑1 (MQ=255)
                                                   ggATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                             <  1:3367198/149‑1 (MQ=255)
                                                    gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctct                                                                                            <  1:890726/149‑1 (MQ=255)
                                                    gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctct                                                                                            <  1:2706259/149‑1 (MQ=255)
                                                     aTAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                           >  2:1239522/1‑149 (MQ=255)
                                                      tAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTc                                                                                          <  1:360432/149‑1 (MQ=255)
                                                       aaCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTcc                                                                                         >  2:880852/1‑149 (MQ=255)
                                                        aCGTTCATAGACCCAACTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                                                                                        <  2:3632104/149‑1 (MQ=255)
                                                        aCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                                                                                        >  1:2460873/1‑149 (MQ=255)
                                                             cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGa                                                                                   <  1:505070/149‑1 (MQ=255)
                                                              aTAGACCCACATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                  <  1:1471624/149‑1 (MQ=255)
                                                              aTAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                  <  1:847293/149‑1 (MQ=255)
                                                              aTAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                  <  2:3924490/149‑1 (MQ=255)
                                                                aGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTg                                                                                >  1:2720383/1‑149 (MQ=255)
                                                                aGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTg                                                                                <  1:3199925/149‑1 (MQ=255)
                                                                    ccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtca                                                                            <  2:1337283/149‑1 (MQ=255)
                                                                    ccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACAATTTATTATATTTATTTAATAAATAATTTTTAATATAAATATAAAGACATTATTAATAAAAAATACGCACAAAACCAGACGCGCTCTCTTCCTGAAGATTGCtca                                                                            <  2:2744708/149‑1 (MQ=255)
                                                                      aaaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                          >  1:1845678/1‑149 (MQ=255)
                                                                      aaaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcata                                                                          >  2:3272850/1‑148 (MQ=255)
                                                                            ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                    >  2:2078902/1‑149 (MQ=255)
                                                                            ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                    >  2:3869133/1‑149 (MQ=255)
                                                                            ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                    <  2:3176531/149‑1 (MQ=255)
                                                                            ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                    <  2:1123249/149‑1 (MQ=255)
                                                                                tGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCa                                                                <  2:2370886/149‑1 (MQ=255)
                                                                                   gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGa                                                             <  1:2929455/149‑1 (MQ=255)
                                                                                   gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGa                                                             <  2:131515/149‑1 (MQ=255)
                                                                                   gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGa                                                             >  2:854789/1‑149 (MQ=255)
                                                                                   gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGa                                                             <  1:165083/149‑1 (MQ=255)
                                                                                    cgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAt                                                            <  1:3336166/149‑1 (MQ=255)
                                                                                      ctgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCa                                                          <  2:974182/149‑1 (MQ=255)
                                                                                      ctgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCa                                                          <  1:153647/149‑1 (MQ=255)
                                                                                        gctgACTAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGc                                                        <  2:487108/149‑1 (MQ=255)
                                                                                        gctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCTAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGc                                                        <  1:2576385/149‑1 (MQ=255)
                                                                                         ctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCa                                                       <  1:589016/149‑1 (MQ=255)
                                                                                          tgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                      >  2:3010375/1‑149 (MQ=255)
                                                                                          tgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                      >  2:2841694/1‑149 (MQ=255)
                                                                                           gACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAcc                                                     >  1:1126023/1‑149 (MQ=255)
                                                                                           gACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAcc                                                     >  1:1999957/1‑149 (MQ=255)
                                                                                                     gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                           >  2:3824975/1‑149 (MQ=255)
                                                                                                      ttCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                          <  2:511345/149‑1 (MQ=255)
                                                                                                      ttCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                          <  2:2579533/149‑1 (MQ=255)
                                                                                                         ggATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                       >  2:1358855/1‑149 (MQ=255)
                                                                                                         ggATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                       >  1:3724614/1‑149 (MQ=255)
                                                                                                          gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTc                                      <  2:3397414/149‑1 (MQ=255)
                                                                                                          gATACCCTTGCGGATCGTTAATTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTc                                      <  1:1319712/149‑1 (MQ=255)
                                                                                                           aTACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCa                                     <  2:1784813/149‑1 (MQ=255)
                                                                                                            tACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAg                                    >  1:1712551/1‑149 (MQ=255)
                                                                                                             aCCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGa                                   >  2:2686168/1‑149 (MQ=255)
                                                                                                               ccTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATa                                 <  2:1501033/149‑1 (MQ=255)
                                                                                                               ccTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCGTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATa                                 <  2:190021/149‑1 (MQ=255)
                                                                                                                cTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAg                                >  2:2949415/1‑149 (MQ=255)
                                                                                                                  tGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGc                              <  1:2495149/149‑1 (MQ=255)
                                                                                                                  tGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGc                              >  2:3671103/1‑149 (MQ=255)
                                                                                                                      gATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATc                          >  1:1470797/1‑149 (MQ=255)
                                                                                                                        tCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAg                        <  2:1091782/149‑1 (MQ=255)
                                                                                                                             aTTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCgg                   >  2:1680598/1‑149 (MQ=255)
                                                                                                                              tttACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                  >  2:1992879/1‑149 (MQ=255)
                                                                                                                              tttACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                  >  2:1753533/1‑149 (MQ=255)
                                                                                                                              tttACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                  >  1:2238803/1‑149 (MQ=255)
                                                                                                                              tttACGACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGATTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                  <  2:1426341/149‑1 (MQ=255)
                                                                                                                                  ctacCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCACCAGACGCACAAAACCAGACGCGCTCTCTTACTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTcaca              <  1:3051997/149‑1 (MQ=255)
                                                                                                                                  ctacCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTcaca              >  2:735640/1‑149 (MQ=255)
                                                                                                                                   tacCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACAt             <  2:446215/149‑1 (MQ=255)
                                                                                                                                   tacCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACAt             <  2:2518814/149‑1 (MQ=255)
                                                                                                                                   tacCGACTTGCTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACAt             >  1:2568161/1‑149 (MQ=255)
                                                                                                                                    acCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATc            >  2:1636179/1‑149 (MQ=255)
                                                                                                                                        aCTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgc        <  1:1497524/149‑1 (MQ=255)
                                                                                                                                        aCTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgc        <  1:1497389/149‑1 (MQ=255)
                                                                                                                                            gTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAg    <  1:1684750/149‑1 (MQ=255)
                                                                                                                                              tCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTc  <  1:3848632/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTC  >  CP009273/3110067‑3110357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑