BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,132,098	A→T	5.8%	T5S (ACT→TCT)	yghU →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,132,098	0	A	T	5.8%	869.8 / 31.4	276	T5S (ACT→TCT)	yghU	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base A (146/114); new base T (9/7); total (155/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 280 total aligned reads displayed.

ACATTAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGAACGCCGA  >  CP009273/3131950‑3132245

aCATTAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATa                                                                                                                                                     <  1:925276/149‑1 (MQ=255)

 cATTAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATAc                                                                                                                                                    >  1:3889526/1‑149 (MQ=255)

 cATTAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATAc                                                                                                                                                    >  2:225732/1‑149 (MQ=255)

  aTTAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACt                                                                                                                                                   <  2:3067486/149‑1 (MQ=255)

  aTTAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACt                                                                                                                                                   >  1:1955750/1‑149 (MQ=255)

   ttAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACtt                                                                                                                                                  >  2:3442307/1‑149 (MQ=255)

      aCCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATc                                                                                                                                               >  2:1404235/1‑149 (MQ=255)

       ccGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCa                                                                                                                                              <  2:2372585/149‑1 (MQ=255)

         ggACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGc                                                                                                                                            <  1:4084932/149‑1 (MQ=255)

           aCAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGccc                                                                                                                                          <  1:2452068/149‑1 (MQ=255)

             agagGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCcgc                                                                                                                                        >  1:3930525/1‑149 (MQ=255)

              gagGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCcgcg                                                                                                                                       >  1:864559/1‑149 (MQ=255)

              gagGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCcgcg                                                                                                                                       >  2:3890802/1‑149 (MQ=255)

                  tCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAg                                                                                                                                   <  1:1666549/149‑1 (MQ=255)

                   cAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGt                                                                                                                                  >  2:272574/1‑149 (MQ=255)

                      aCTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTg                                                                                                                               >  2:1397912/1‑149 (MQ=255)

                      aCTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTg                                                                                                                               >  2:3423190/1‑149 (MQ=255)

                         aaTCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATTCTTATCAGCCCGCGAAAGTCTGGAc                                                                                                                            >  2:2012942/1‑149 (MQ=255)

                           tCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGt                                                                                                                          <  1:2252842/149‑1 (MQ=255)

                            cATACACCTCGTCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTg                                                                                                                         <  1:1652292/149‑1 (MQ=255)

                             aTACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTgg                                                                                                                        >  1:2826742/1‑149 (MQ=255)

                             aTACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTgg                                                                                                                        >  2:3522502/1‑149 (MQ=255)

                               acacCTCATCGCAGCGGCTGGCGCAAAGTTTTAAAAAATTCACATTCTGTACAGAAAGTGACCTGCTACACTGCTTCAACACTACCACTAAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAATTCTGGACGTGGGa                                                                                                                      <  2:2653748/149‑1 (MQ=255)

                               acacCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGa                                                                                                                      >  1:2303814/1‑149 (MQ=255)

                                  ccTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATaa                                                                                                                   >  1:3346437/1‑149 (MQ=255)

                                    tCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATAATTATCAGCCCGCGAAATTCTGGACGTGGGATAAAt                                                                                                                 >  1:2480189/1‑149 (MQ=255)

                                      aTCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATcc                                                                                                               <  2:3964568/149‑1 (MQ=255)

                                       tCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCg                                                                                                              >  2:1062911/1‑149 (MQ=255)

                                        cGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGc                                                                                                             <  1:4061749/149‑1 (MQ=255)

                                           aGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTgg                                                                                                          <  1:2997816/149‑1 (MQ=255)

                                           aGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTgg                                                                                                          <  1:1418926/149‑1 (MQ=255)

                                            gCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTggc                                                                                                         >  1:1438084/1‑149 (MQ=255)

                                            gCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTggc                                                                                                         >  1:3485768/1‑149 (MQ=255)

                                            gCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGACCGCGAAAGTCTGGACGTGGGATAAATCCGCTggc                                                                                                         >  1:2211407/1‑149 (MQ=255)

                                             cGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTggcg                                                                                                        <  2:3862500/149‑1 (MQ=255)

                                              ggCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTggcgg                                                                                                       <  1:3715199/149‑1 (MQ=255)

                                                    cgcAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGtt                                                                                                 <  1:515434/149‑1 (MQ=255)

                                                     gcAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTc                                                                                                <  1:1564943/149‑1 (MQ=255)

                                                         aGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCa                                                                                            >  2:2473317/1‑149 (MQ=255)

                                                         aGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCa                                                                                            >  2:2473417/1‑149 (MQ=255)

                                                            gTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAata                                                                                         >  1:4038764/1‑149 (MQ=255)

                                                            gTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTAGCCAata                                                                                         >  2:2928552/1‑149 (MQ=255)

                                                              tAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATc                                                                                       >  1:786054/1‑149 (MQ=255)

                                                              tAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATc                                                                                       <  1:1861274/149‑1 (MQ=255)

                                                                 aaaaTTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAAt                                                                                    >  2:3743732/1‑149 (MQ=255)

                                                                 aaaaTTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAAt                                                                                    <  1:883010/149‑1 (MQ=255)

                                                                 aaaaTTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAAt                                                                                    >  2:4084765/1‑149 (MQ=255)

                                                                   aaTTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCg                                                                                  >  2:3008824/1‑149 (MQ=255)

                                                                     ttCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGcc                                                                                <  1:1186017/149‑1 (MQ=255)

                                                                     ttCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGcc                                                                                >  1:1097457/1‑149 (MQ=255)

                                                                        acaTTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCgg                                                                             >  2:1617493/1‑149 (MQ=255)

                                                                        acaTTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCgg                                                                             >  1:2048189/1‑149 (MQ=255)

                                                                        acaTTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCgg                                                                             >  1:1778869/1‑149 (MQ=255)

                                                                         caTTGTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGt                                                                            <  2:40161/149‑1 (MQ=255)

                                                                         caTTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGt                                                                            <  2:294774/149‑1 (MQ=255)

                                                                          aTTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGtt                                                                           >  2:3042809/1‑149 (MQ=255)

                                                                          aTTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGtt                                                                           <  2:335120/149‑1 (MQ=255)

                                                                            tCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTc                                                                         >  1:1739180/1‑149 (MQ=255)

                                                                             cTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACACTACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCt                                                                        <  2:3148887/149‑1 (MQ=255)

                                                                               gTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTgg                                                                      <  2:1706240/149‑1 (MQ=255)

                                                                                  cAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTcc                                                                   >  2:1320405/1‑149 (MQ=255)

                                                                                    gCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGa                                                                 >  1:2732268/1‑149 (MQ=255)

                                                                                        gTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCa                                                             >  1:2884361/1‑149 (MQ=255)

                                                                                          gACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATg                                                           >  2:3207684/1‑149 (MQ=255)

                                                                                              tGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGaaaa                                                       >  2:1820230/1‑149 (MQ=255)

                                                                                                 tACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAAc                                                    <  1:3418284/149‑1 (MQ=255)

                                                                                                   cacTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATTCTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGc                                                  <  1:3259579/149‑1 (MQ=255)

                                                                                                    acTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCt                                                 <  2:3485768/149‑1 (MQ=255)

                                                                                                     cTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTg                                                >  1:2787737/1‑149 (MQ=255)

                                                                                                         ttCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCg                                            >  1:95467/1‑149 (MQ=255)

                                                                                                           cAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGtt                                          <  1:3385501/149‑1 (MQ=255)

                                                                                                           cAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGtt                                          <  1:1336514/149‑1 (MQ=255)

                                                                                                                  aCCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAac                                   >  2:2205567/1‑149 (MQ=255)

                                                                                                                  aCCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAac                                   >  2:955469/1‑149 (MQ=255)

                                                                                                                   ccACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAaca                                  <  2:786054/149‑1 (MQ=255)

                                                                                                                    cACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAacac                                 >  1:3535716/1‑149 (MQ=255)

                                                                                                                       tCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCa                              >  2:680258/1‑149 (MQ=255)

                                                                                                                          gAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTg                           >  2:2818934/1‑149 (MQ=255)

                                                                                                                          gAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTg                           >  1:2950288/1‑149 (MQ=255)

                                                                                                                          gAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTg                           >  1:3168291/1‑149 (MQ=255)

                                                                                                                          gAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTg                           <  2:1955768/149‑1 (MQ=255)

                                                                                                                          gAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTg                           <  2:1532924/149‑1 (MQ=255)

                                                                                                                           aaGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGc                          <  2:1405428/149‑1 (MQ=255)

                                                                                                                              gCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAAc                       >  1:150769/1‑149 (MQ=255)

                                                                                                                                aaCTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACtt                     <  1:744787/149‑1 (MQ=255)

                                                                                                                                aaCTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACtt                     >  1:533397/1‑149 (MQ=255)

                                                                                                                                 aCTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACttt                    >  2:3204780/1‑149 (MQ=255)

                                                                                                                                   tCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTAt                  >  2:3286146/1‑149 (MQ=255)

                                                                                                                                    cACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTAtt                 <  1:2664716/149‑1 (MQ=255)

                                                                                                                                    cACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTAtt                 <  1:3945337/149‑1 (MQ=255)

                                                                                                                                      cTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCg               >  1:2104575/1‑149 (MQ=255)

                                                                                                                                       tATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGc              >  2:1855498/1‑149 (MQ=255)

                                                                                                                                          gacagacaATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGTAAACACCCATTGCAACTTTATTCGCTgg           >  2:415546/1‑149 (MQ=255)

                                                                                                                                          gacagacaATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTgg           >  2:1447945/1‑149 (MQ=255)

                                                                                                                                          gacagacaATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTgg           >  1:3090769/1‑149 (MQ=255)

                                                                                                                                             agacaATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGaa        <  2:4066249/149‑1 (MQ=255)

                                                                                                                                                caATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGAACGc     <  2:1119248/149‑1 (MQ=255)

                                                                                                                                                 aaTACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGAACGcc    >  1:3836888/1‑149 (MQ=255)

                                                                                                                                                  aTACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGAACGCCg   >  1:2430187/1‑149 (MQ=255)

                                                                                                                                                   tACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGAACGCCGa  <  1:3207684/149‑1 (MQ=255)

ACATTAACCGGACAGAGGTCAGACTAATCATACACCTCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTACCACTCAGAAGGCAACTCACTATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCGCCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGAACGCCGA  >  CP009273/3131950‑3132245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`