Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,134,528 C→A 100% D40Y (GAT→TAT)  hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,134,5280CA97.2% 813.5 / 9.5 267D40Y (GAT→TAT) hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base A (127/132);  total (129/138)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

GTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTT  >  CP009273/3134382‑3134674
                                                                                                                                                  |                                                                                                                                                  
gttgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGa                                                                                                                                                  <  1:3550717/149‑1 (MQ=255)
  tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAgg                                                                                                                                                <  1:4103145/149‑1 (MQ=255)
  tgGGAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCCTCGTTCCCGGCGCGTTCTTTTAAGAGACAATGGCATCCGCAATAATCAACTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAgg                                                                                                                                                <  1:1444061/149‑1 (MQ=255)
   ggTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGa                                                                                                                                               >  2:1171735/1‑149 (MQ=255)
    gTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAt                                                                                                                                              >  1:1404721/1‑149 (MQ=255)
     tAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtc                                                                                                                                             <  1:2109292/149‑1 (MQ=255)
       aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtctc                                                                                                                                           <  2:3254873/149‑1 (MQ=255)
       aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtctc                                                                                                                                           <  2:1229999/149‑1 (MQ=255)
         caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCaa                                                                                                                                         <  1:2915643/149‑1 (MQ=255)
         caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCaa                                                                                                                                         <  1:3010499/149‑1 (MQ=255)
           acGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACa                                                                                                                                       >  2:959347/1‑149 (MQ=255)
               cGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAAt                                                                                                                                   <  1:2735392/149‑1 (MQ=255)
                ggAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATc                                                                                                                                  >  1:1947548/1‑149 (MQ=255)
                 gAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATcc                                                                                                                                 >  1:1823755/1‑149 (MQ=255)
                   aCTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCgg                                                                                                                               >  1:3075971/1‑149 (MQ=255)
                           aTCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGt                                                                                                                       >  2:1647604/1‑149 (MQ=255)
                             cccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                                                     <  1:136331/149‑1 (MQ=255)
                                gCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGt                                                                                                                  <  1:2818705/149‑1 (MQ=255)
                                  aGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATTATCAAATGATCGCGATTTGCCATGTCGCGAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTg                                                                                                                <  1:2621637/149‑1 (MQ=255)
                                   ggATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGc                                                                                                               <  1:899451/149‑1 (MQ=255)
                                   ggATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGc                                                                                                               >  2:1904767/1‑149 (MQ=255)
                                   ggATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGc                                                                                                               <  1:498641/149‑1 (MQ=255)
                                      tcatcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctct                                                                                                            >  2:567204/1‑149 (MQ=255)
                                      tcatcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctct                                                                                                            >  1:2856278/1‑149 (MQ=255)
                                           atcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGc                                                                                                       <  1:2222099/149‑1 (MQ=255)
                                             cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCtt                                                                                                     >  2:506117/1‑149 (MQ=255)
                                               ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCg                                                                                                   >  2:3043391/1‑149 (MQ=255)
                                                   cGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACaa                                                                                               <  2:4100516/149‑1 (MQ=255)
                                                   cGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACaa                                                                                               <  1:1888149/149‑1 (MQ=255)
                                                     gcgcGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATc                                                                                             >  1:968932/1‑149 (MQ=255)
                                                       gcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCg                                                                                           >  2:1293169/1‑149 (MQ=255)
                                                          ttCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcac                                                                                        >  2:3659879/1‑149 (MQ=255)
                                                          ttCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTGGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCAGAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcac                                                                                        <  1:1861111/149‑1 (MQ=255)
                                                             tttttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACAcc                                                                                     >  1:2370270/1‑149 (MQ=255)
                                                                ttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGAt                                                                                  <  2:2532557/149‑1 (MQ=255)
                                                                 tCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATg                                                                                 <  2:3072737/149‑1 (MQ=255)
                                                                   gagaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGc                                                                               >  1:3168954/1‑149 (MQ=255)
                                                                   gagaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGc                                                                               >  1:3213219/1‑149 (MQ=255)
                                                                   gagaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGc                                                                               <  2:2792959/149‑1 (MQ=255)
                                                                       cAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                           <  2:2703249/149‑1 (MQ=255)
                                                                         aTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATc                                                                         <  1:2757259/149‑1 (MQ=255)
                                                                         aTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATc                                                                         <  2:2739206/149‑1 (MQ=255)
                                                                         aTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATc                                                                         <  2:3960097/149‑1 (MQ=255)
                                                                           ggCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCgg                                                                       >  1:1578687/1‑149 (MQ=255)
                                                                            gCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGt                                                                      >  2:1779420/1‑149 (MQ=255)
                                                                              aTCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCa                                                                    <  1:815238/149‑1 (MQ=255)
                                                                                    aataatCACATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaaa                                                              <  2:3090543/149‑1 (MQ=255)
                                                                                     ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGTCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                             >  1:1517968/1‑149 (MQ=255)
                                                                                     ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                             <  1:2219198/149‑1 (MQ=255)
                                                                                     ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                             >  1:2390003/1‑149 (MQ=255)
                                                                                      taatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAata                                                            >  2:1705190/1‑149 (MQ=255)
                                                                                      taatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAata                                                            <  1:3044364/149‑1 (MQ=255)
                                                                                        atCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATAtt                                                          >  2:30018/1‑149 (MQ=255)
                                                                                          cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGc                                                        >  1:3280546/1‑149 (MQ=255)
                                                                                           aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGcc                                                       <  2:750911/149‑1 (MQ=255)
                                                                                           aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGcc                                                       >  1:1924689/1‑149 (MQ=255)
                                                                                            aaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                      >  2:1518071/1‑149 (MQ=255)
                                                                                               gATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcc                                                   >  2:2017315/1‑149 (MQ=255)
                                                                                                 tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                                 >  2:1355156/1‑149 (MQ=255)
                                                                                                 tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                                 <  2:1483046/149‑1 (MQ=255)
                                                                                                 tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                                 >  1:3948749/1‑149 (MQ=255)
                                                                                                 tCGCGATTTGCCATGTCGCCAAGCAGCTCCAGTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                                 <  2:4003529/149‑1 (MQ=255)
                                                                                                  cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCt                                                <  1:2965685/149‑1 (MQ=255)
                                                                                                  cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCt                                                <  1:1907559/149‑1 (MQ=255)
                                                                                                   gcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTa                                               >  1:3152244/1‑149 (MQ=255)
                                                                                                      aTTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                            <  2:2161275/149‑1 (MQ=255)
                                                                                                        ttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACt                                          <  1:1707200/149‑1 (MQ=255)
                                                                                                             aTGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAAt                                     <  2:904210/149‑1 (MQ=255)
                                                                                                             aTGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAAt                                     <  2:1530153/149‑1 (MQ=255)
                                                                                                             aTGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAAt                                     <  2:1831244/149‑1 (MQ=255)
                                                                                                             aTGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAAt                                     >  1:4088179/1‑149 (MQ=255)
                                                                                                             aTGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCGTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTACGACTAACAt                                     <  2:680580/149‑1 (MQ=255)
                                                                                                              tGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                    <  2:171589/149‑1 (MQ=255)
                                                                                                              tGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                    >  1:3605886/1‑149 (MQ=255)
                                                                                                               gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATAc                                   <  1:1840569/149‑1 (MQ=255)
                                                                                                                tCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg                                  <  1:416787/149‑1 (MQ=255)
                                                                                                                 cGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGc                                 <  1:1647604/149‑1 (MQ=255)
                                                                                                                  gCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCa                                >  1:1303719/1‑149 (MQ=255)
                                                                                                                     aaGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa                             <  1:662528/149‑1 (MQ=255)
                                                                                                                      agcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc                            <  1:1331109/149‑1 (MQ=255)
                                                                                                                      agcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc                            >  1:2463701/1‑149 (MQ=255)
                                                                                                                       gcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa                           >  2:1221431/1‑149 (MQ=255)
                                                                                                                       gcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa                           >  2:2127857/1‑149 (MQ=255)
                                                                                                                          gcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGaa                        <  2:3731251/149‑1 (MQ=255)
                                                                                                                           cTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAc                       <  1:1760180/149‑1 (MQ=255)
                                                                                                                            tCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAcc                      >  1:3872071/1‑149 (MQ=255)
                                                                                                                             ccATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCt                     >  2:3415852/1‑149 (MQ=255)
                                                                                                                              cATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt                    <  2:289903/149‑1 (MQ=255)
                                                                                                                                ggcccGCCGTGCCGCCAGCGACGATCTCCACCTCATCCGGCAGAAGGTATCGTGGCTCTAACGCTTCGACCATCCGCACACCGATGGCTTCATCGGTCAGCAAAATAGTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCa                  <  2:2935208/147‑1 (MQ=255)
                                                                                                                                 tCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCAc                 <  2:3948607/149‑1 (MQ=255)
                                                                                                                                 tCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCAc                 <  2:2856278/149‑1 (MQ=255)
                                                                                                                                  cccGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACt                >  1:3283128/1‑149 (MQ=255)
                                                                                                                                  cccGCCGTGACGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACt                <  1:3634704/149‑1 (MQ=255)
                                                                                                                                      ccgTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGaaa            >  2:1751573/1‑149 (MQ=255)
                                                                                                                                        gTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAcc          <  1:152921/149‑1 (MQ=255)
                                                                                                                                          gccgccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCAc        <  1:100755/149‑1 (MQ=255)
                                                                                                                                           ccgccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt       <  1:376236/149‑1 (MQ=255)
                                                                                                                                             gccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc     >  1:1011156/1‑149 (MQ=255)
                                                                                                                                              ccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg    <  1:959347/149‑1 (MQ=255)
                                                                                                                                               cATAGAGGATCTCAACAGAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGt   <  2:3161748/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTT  >  CP009273/3134382‑3134674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑