BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,169,188	C→A	48.2%	G60C (GGT→TGT)	yqiA ←	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,169,188	0	C	A	48.2%	101.5 / 252.1	193	G60C (GGT→TGT)	yqiA	acyl CoA esterase
Reads supporting (aligned to +/- strand): ref base C (64/36); new base A (41/52); total (105/88)
Fisher's exact test for biased strand distribution p-value = 6.20e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 197 total aligned reads displayed.

GGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTG  >  CP009273/3169041‑3169336

ggTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCg                                                                                                                                                     >  1:1657690/1‑149 (MQ=255)

 gTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGc                                                                                                                                                    <  2:2364820/149‑1 (MQ=255)

     tcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATg                                                                                                                                                >  2:1464431/1‑149 (MQ=255)

     tcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATg                                                                                                                                                <  2:2255312/149‑1 (MQ=255)

       gTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGtt                                                                                                                                              <  2:272685/149‑1 (MQ=255)

        ttCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTc                                                                                                                                             >  1:1943811/1‑149 (MQ=255)

           tGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAg                                                                                                                                          <  2:891240/149‑1 (MQ=255)

            gACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAgg                                                                                                                                         >  2:1402821/1‑149 (MQ=255)

             aCCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGa                                                                                                                                        >  1:2408488/1‑149 (MQ=255)

               cGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACa                                                                                                                                      >  1:2590325/1‑149 (MQ=255)

                gagaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACaa                                                                                                                                     >  2:3197456/1‑149 (MQ=255)

                 agaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAGCCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAAt                                                                                                                                    >  1:3515371/1‑149 (MQ=255)

                 agaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAAt                                                                                                                                    >  1:2110413/1‑149 (MQ=255)

                 agaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAAt                                                                                                                                    >  2:3522518/1‑149 (MQ=255)

                  gaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATg                                                                                                                                   >  1:3721092/1‑149 (MQ=255)

                    tAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGa                                                                                                                                 <  1:1921629/149‑1 (MQ=255)

                          gTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCcag                                                                                                                           >  1:990377/1‑149 (MQ=255)

                            cagcagTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCcagca                                                                                                                         >  2:1197928/1‑149 (MQ=255)

                            cagcagTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCcagca                                                                                                                         >  2:3248232/1‑149 (MQ=255)

                              gcagTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCagcagc                                                                                                                       >  1:1373637/1‑149 (MQ=255)

                                  ttCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTg                                                                                                                   >  2:2044616/1‑149 (MQ=255)

                                   tCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGc                                                                                                                  <  1:3794576/149‑1 (MQ=255)

                                   tCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGc                                                                                                                  <  2:404046/149‑1 (MQ=255)

                                         gggCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACCATGGATTCCAGCAGCTCTGCCGCGTc                                                                                                            >  2:757183/1‑149 (MQ=255)

                                             gcACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGaa                                                                                                        >  2:1436180/1‑149 (MQ=255)

                                               aCCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAgg                                                                                                      <  2:2392168/149‑1 (MQ=255)

                                               aCCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAgg                                                                                                      <  2:761479/149‑1 (MQ=255)

                                                  gccgGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATa                                                                                                   >  1:3488332/1‑149 (MQ=255)

                                                    cgGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATAcg                                                                                                 <  2:3928437/149‑1 (MQ=255)

                                                     gggTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATAcgg                                                                                                >  1:320836/1‑149 (MQ=255)

                                                      ggTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATActgc                                                                                               >  1:2872080/1‑149 (MQ=255)

                                                       gTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATAcggcg                                                                                              >  1:2653982/1‑149 (MQ=255)

                                                        tttACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACNATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATAcggcgg                                                                                             >  1:2085661/1‑149 (MQ=255)

                                                            ccaccaCTGCGGGCAGCATAACACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAAc                                                                                         <  2:3336234/149‑1 (MQ=255)

                                                            ccaccaCTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAAc                                                                                         >  1:3968358/1‑149 (MQ=255)

                                                            ccaccaCTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAAc                                                                                         <  2:3152700/149‑1 (MQ=255)

                                                              accacTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATCTCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTg                                                                                       >  1:1440099/1‑149 (MQ=255)

                                                                cacTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCg                                                                                     >  1:237459/1‑149 (MQ=255)

                                                                cacTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCg                                                                                     >  1:3123815/1‑149 (MQ=255)

                                                                   tGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGaa                                                                                  >  1:3059205/1‑149 (MQ=255)

                                                                    gCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAAt                                                                                 <  1:3452150/149‑1 (MQ=255)

                                                                      gggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGACTGa                                                                               >  1:39177/1‑149 (MQ=255)

                                                                      gggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGa                                                                               >  2:1687509/1‑149 (MQ=255)

                                                                       ggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGat                                                                              <  1:1897583/149‑1 (MQ=255)

                                                                          agcaTAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGatcat                                                                           <  1:3611037/149‑1 (MQ=255)

                                                                               aaaaCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCaa                                                                      >  2:3059927/1‑149 (MQ=255)

                                                                               aaaaCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCaa                                                                      <  1:1971248/149‑1 (MQ=255)

                                                                                  aCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGt                                                                   >  1:3712008/1‑149 (MQ=255)

                                                                                  aCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGt                                                                   >  1:1596017/1‑149 (MQ=255)

                                                                                  aCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGt                                                                   >  1:1596081/1‑149 (MQ=255)

                                                                                   cATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTc                                                                  <  1:423568/149‑1 (MQ=255)

                                                                                   cATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTc                                                                  <  1:1275966/149‑1 (MQ=255)

                                                                                    aTTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGGTCATCTCAACGTCa                                                                 >  2:3935343/1‑149 (MQ=255)

                                                                                     ttGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAg                                                                >  2:1580648/1‑149 (MQ=255)

                                                                                      tgtgACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAgg                                                               <  1:854645/149‑1 (MQ=255)

                                                                                         gACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCACCTGCGGAATGATCATCTCAACGTCAGGGTg                                                            >  1:2201747/1‑149 (MQ=255)

                                                                                           cAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGAt                                                          <  2:3841431/149‑1 (MQ=255)

                                                                                            aaCCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGACTGATCATCTCAACGTCAGGGTGATg                                                         >  2:2115318/1‑149 (MQ=255)

                                                                                             aCCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGt                                                        >  2:365679/1‑149 (MQ=255)

                                                                                             aCCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGt                                                        <  1:3967692/149‑1 (MQ=255)

                                                                                                aGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTcc                                                     <  1:4033775/149‑1 (MQ=255)

                                                                                                  gTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCgc                                                   >  1:3250041/1‑149 (MQ=255)

                                                                                                   tGGCGTAATATCCCCCCCGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCgcc                                                  <  2:652281/149‑1 (MQ=255)

                                                                                                       gTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCgccagcc                                              <  2:3800801/149‑1 (MQ=255)

                                                                                                         aaTATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGccagccag                                            <  2:1943811/149‑1 (MQ=255)

                                                                                                          atatCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGt                                           <  1:412944/149‑1 (MQ=255)

                                                                                                           tatCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGtt                                          <  2:3769951/149‑1 (MQ=255)

                                                                                                           tatCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGtt                                          >  2:4079608/1‑149 (MQ=255)

                                                                                                           tatCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGtt                                          >  2:1473175/1‑149 (MQ=255)

                                                                                                            atCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttt                                         <  1:2573240/149‑1 (MQ=255)

                                                                                                            atCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttt                                         >  1:3409249/1‑149 (MQ=255)

                                                                                                            atCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttt                                         <  1:1910923/149‑1 (MQ=255)

                                                                                                              ccccccAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttttt                                       <  1:1001031/149‑1 (MQ=255)

                                                                                                              ccccccAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttttt                                       <  1:1000974/149‑1 (MQ=255)

                                                                                                                ccccAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTa                                     <  2:3524025/149‑1 (MQ=255)

                                                                                                                      tGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGc                               <  2:2948716/149‑1 (MQ=255)

                                                                                                                      tGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGc                               <  2:470695/149‑1 (MQ=255)

                                                                                                                      tGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGc                               >  1:3255001/1‑149 (MQ=255)

                                                                                                                        aCGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTc                             >  2:1139062/1‑149 (MQ=255)

                                                                                                                            aCCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCtt                         <  2:3883756/149‑1 (MQ=255)

                                                                                                                             ccGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCttt                        <  2:3488332/149‑1 (MQ=255)

                                                                                                                             ccGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCttt                        >  1:892198/1‑149 (MQ=255)

                                                                                                                                aCAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGc                     >  2:3119735/1‑149 (MQ=255)

                                                                                                                                  aaTACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCag                   >  2:1441357/1‑149 (MQ=255)

                                                                                                                                   aTACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCaga                  >  1:2728927/1‑149 (MQ=255)

                                                                                                                                    tACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCagag                 >  1:2854780/1‑149 (MQ=255)

                                                                                                                                       ccAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAgcgc              <  2:3066779/149‑1 (MQ=255)

                                                                                                                                       ccAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAgcgc              >  2:2905834/1‑149 (MQ=255)

                                                                                                                                       ccAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAgcgc              <  2:1273552/149‑1 (MQ=255)

                                                                                                                                       ccAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAgcgc              <  1:1436180/149‑1 (MQ=255)

                                                                                                                                        cAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCGTTTGCAGAGcgcg             >  2:2883909/1‑149 (MQ=255)

                                                                                                                                          gCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCgcggc           <  1:1311594/149‑1 (MQ=255)

                                                                                                                                          gCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCgcggc           >  2:1509946/1‑149 (MQ=255)

                                                                                                                                            gAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGa         <  2:3637082/149‑1 (MQ=255)

                                                                                                                                             aaTCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAg        >  1:948105/1‑149 (MQ=255)

                                                                                                                                             aaTCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAg        >  1:3007400/1‑149 (MQ=255)

                                                                                                                                             aaTCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAg        >  2:1731192/1‑149 (MQ=255)

                                                                                                                                              aTCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGc       >  2:3378386/1‑149 (MQ=255)

                                                                                                                                                  ccGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGtt   >  1:4050149/1‑149 (MQ=255)

                                                                                                                                                   cGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTg  >  2:2984984/1‑149 (MQ=255)

GGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTTG  >  CP009273/3169041‑3169336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`