Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 29,139 G→T 100% G256C (GGT→TGT)  dapB → dihydrodipicolinate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927329,1390GT100.0% 979.2 / ‑6.7 292G256C (GGT→TGT) dapBdihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (94/197);  total (94/198)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 292 total aligned reads displayed.

TTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGT  >  CP009273/28996‑29284
                                                                                                                                               |                                                                                                                                                 
 tGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAgg                                                                                                                                             <  2:4002761/149‑1 (MQ=255)
 tGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAgg                                                                                                                                             >  1:2245536/1‑149 (MQ=255)
   ccACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGaa                                                                                                                                           >  1:929593/1‑149 (MQ=255)
    cACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGaaa                                                                                                                                          <  2:3268789/149‑1 (MQ=255)
     aCCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAg                                                                                                                                         >  2:689725/1‑149 (MQ=255)
     aCCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAg                                                                                                                                         <  2:2709508/149‑1 (MQ=255)
     aCCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAg                                                                                                                                         >  1:2622385/1‑149 (MQ=255)
      ccGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGc                                                                                                                                        <  2:35266/149‑1 (MQ=255)
      ccGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGc                                                                                                                                        <  1:2115337/149‑1 (MQ=255)
           cgtgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCt                                                                                                                                   <  2:379203/149‑1 (MQ=255)
           cgtgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCt                                                                                                                                   <  2:3023094/149‑1 (MQ=255)
              gcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCtttt                                                                                                                                <  2:1932119/149‑1 (MQ=255)
                 ggTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGa                                                                                                                             <  2:1843277/149‑1 (MQ=255)
                  gTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGat                                                                                                                            >  2:3509962/1‑149 (MQ=255)
                     aCATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATg                                                                                                                         >  1:1133877/1‑149 (MQ=255)
                        tCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCga                                                                                                                      >  2:2120506/1‑149 (MQ=255)
                          gTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCgaga                                                                                                                    <  1:3544539/149‑1 (MQ=255)
                          gTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCgaga                                                                                                                    <  1:1081973/149‑1 (MQ=255)
                             ggTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGt                                                                                                                 <  2:2833749/149‑1 (MQ=255)
                             ggTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGt                                                                                                                 >  2:3585352/1‑149 (MQ=255)
                                     tACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGAtc                                                                                                         <  1:1639675/149‑1 (MQ=255)
                                     tACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGAtc                                                                                                         <  2:4088901/149‑1 (MQ=255)
                                         gcgATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaa                                                                                                     <  1:2353336/149‑1 (MQ=255)
                                         gcgATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaa                                                                                                     <  2:1988571/149‑1 (MQ=255)
                                           gATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaata                                                                                                   >  1:2934443/1‑149 (MQ=255)
                                             tGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaataat                                                                                                 <  1:2355106/149‑1 (MQ=255)
                                             tGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaataat                                                                                                 <  1:3005705/149‑1 (MQ=255)
                                              gTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAAtt                                                                                                <  1:1204215/149‑1 (MQ=255)
                                              gTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAAtt                                                                                                <  1:3344243/149‑1 (MQ=255)
                                              gTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAAtt                                                                                                <  1:1675022/149‑1 (MQ=255)
                                              gTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAAtt                                                                                                <  1:2443846/149‑1 (MQ=255)
                                                   ccGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTaa                                                                                           <  1:3872668/149‑1 (MQ=255)
                                                     gATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAAcc                                                                                         <  1:1874250/149‑1 (MQ=255)
                                                       tatTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACcac                                                                                       >  2:2043274/1‑149 (MQ=255)
                                                          tGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACaaa                                                                                    >  2:1450869/1‑149 (MQ=255)
                                                           ggCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACaaaa                                                                                   <  1:5529/149‑1 (MQ=255)
                                                           ggCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACaaaa                                                                                   >  1:3699179/1‑149 (MQ=255)
                                                               aGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATAtt                                                                               >  2:3189745/1‑149 (MQ=255)
                                                               aGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATAtt                                                                               >  2:573911/1‑149 (MQ=255)
                                                                  gTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGt                                                                            <  1:2596318/149‑1 (MQ=255)
                                                                  gTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGt                                                                            >  2:1798118/1‑149 (MQ=255)
                                                                  gTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGt                                                                            <  2:3490466/149‑1 (MQ=255)
                                                                  gTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGt                                                                            <  1:1503054/149‑1 (MQ=255)
                                                                   tCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGtt                                                                           <  1:1305322/149‑1 (MQ=255)
                                                                   tCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGtt                                                                           >  1:2511249/1‑149 (MQ=255)
                                                                    cTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTa                                                                          <  1:533649/149‑1 (MQ=255)
                                                                      ggAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATg                                                                        <  1:76027/149‑1 (MQ=255)
                                                                            cACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCaa                                                                  >  1:1672617/1‑149 (MQ=255)
                                                                                cATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAAt                                                              <  1:1156029/149‑1 (MQ=255)
                                                                                 aTAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATa                                                             <  1:1780065/149‑1 (MQ=255)
                                                                                   aaGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAac                                                           <  1:2874316/149‑1 (MQ=255)
                                                                                   aaGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAac                                                           <  1:1254151/149‑1 (MQ=255)
                                                                                   aaGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAac                                                           <  1:3471793/149‑1 (MQ=255)
                                                                                    aGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAaca                                                          <  1:2017467/149‑1 (MQ=255)
                                                                                       cGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACat                                                       <  1:3107029/149‑1 (MQ=255)
                                                                                        gTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATACTTTGTAACGACAAACTATTGGTTATGGTGCACAAATAACACatt                                                      >  2:2618032/1‑149 (MQ=255)
                                                                                         tCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACattt                                                     <  2:133460/149‑1 (MQ=255)
                                                                                           cAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaa                                                   <  2:3433812/149‑1 (MQ=255)
                                                                                           cAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaa                                                   <  2:361520/149‑1 (MQ=255)
                                                                                            aGCCGTATGACATTTGCTCACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaat                                                  <  2:2270757/149‑1 (MQ=255)
                                                                                            aGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaat                                                  <  2:3874383/149‑1 (MQ=255)
                                                                                             gCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaatt                                                 >  1:1224955/1‑149 (MQ=255)
                                                                                              ccGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaattt                                                <  2:1722085/149‑1 (MQ=255)
                                                                                                gTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTAt                                              >  2:2988028/1‑149 (MQ=255)
                                                                                                 tATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTAtt                                             >  1:2198248/1‑149 (MQ=255)
                                                                                                  aTGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTg                                            <  1:3509725/149‑1 (MQ=255)
                                                                                                  aTGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTg                                            <  1:1450869/149‑1 (MQ=255)
                                                                                                   tGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGa                                           >  1:1954253/1‑149 (MQ=255)
                                                                                                    gACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAt                                          <  1:3339786/149‑1 (MQ=255)
                                                                                                     aCATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAtt                                         <  1:1697343/149‑1 (MQ=255)
                                                                                                      cATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATta                                        <  1:456004/149‑1 (MQ=255)
                                                                                                       aTTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATtat                                       <  1:4065355/149‑1 (MQ=255)
                                                                                                               aCGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCt                               >  1:1323219/1‑149 (MQ=255)
                                                                                                                 ggCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCttt                             <  1:1846902/149‑1 (MQ=255)
                                                                                                                 ggCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCttt                             <  1:1152444/149‑1 (MQ=255)
                                                                                                                  gcgcGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTa                            >  1:3296483/1‑149 (MQ=255)
                                                                                                                   cgcgGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTaa                           <  1:3485078/149‑1 (MQ=255)
                                                                                                                    tctgTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAt                          <  2:2480550/146‑1 (MQ=255)
                                                                                                                    gcgGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAt                          <  1:716583/149‑1 (MQ=255)
                                                                                                                     cgGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAtt                         <  2:1640995/149‑1 (MQ=255)
                                                                                                                      ggTAAGAGCGGCTTTGTGGTTGAGTGGTAAGCAAAGCGGTCTATTTGATATGCGAGATGGACGTGATCTCAATAATTTGTAACCACAAAATATTTGTTATCGTGCAAAAATAACACAATTAATTGATTGATTATAAAGGGCTTTAAttt                        <  2:2445097/149‑1 (MQ=255)
                                                                                                                       gTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAtttt                       >  1:2775261/1‑149 (MQ=255)
                                                                                                                       gTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAtttt                       <  1:2321795/149‑1 (MQ=255)
                                                                                                                       gTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAtttt                       <  1:654209/149‑1 (MQ=255)
                                                                                                                        tAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAAACACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAttttt                      >  1:861510/1‑149 (MQ=255)
                                                                                                                         aaGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTg                     <  2:51440/149‑1 (MQ=255)
                                                                                                                         aaGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTg                     <  2:3854417/149‑1 (MQ=255)
                                                                                                                         aaGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTg                     <  2:1149992/149‑1 (MQ=255)
                                                                                                                         aaGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTg                     <  2:3034742/149‑1 (MQ=255)
                                                                                                                             tCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTTGccc                 >  2:1124114/1‑149 (MQ=255)
                                                                                                                             tCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGccc                 >  2:1384151/1‑149 (MQ=255)
                                                                                                                             tCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGccc                 >  1:3226779/1‑149 (MQ=255)
                                                                                                                               ggCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGATATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGTCCCtt               >  2:2383374/1‑149 (MQ=255)
                                                                                                                                gCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCttt              <  1:611344/149‑1 (MQ=255)
                                                                                                                                   ttGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTAt           <  2:2981068/149‑1 (MQ=255)
                                                                                                                                     gtgGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTAttt         >  1:3185460/1‑149 (MQ=255)
                                                                                                                                      tgGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTAtttt        >  2:1605414/1‑149 (MQ=255)
                                                                                                                                       ggTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTAttttt       <  2:251174/149‑1 (MQ=255)
                                                                                                                                        gTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTg      >  1:1987282/1‑149 (MQ=255)
                                                                                                                                         ttGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTgg     <  2:1618813/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGT  >  CP009273/28996‑29284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑