Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,184,211 C→A 100% S74Y (TCT→TAT)  yqiI → fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,184,2110CA98.1% 1023.6 / 7.9 319S74Y (TCT→TAT) yqiIfimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (145/168);  total (150/169)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 319 total aligned reads displayed.

GCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCA  >  CP009273/3184066‑3184356
                                                                                                                                                 |                                                                                                                                                 
 cTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCa                                                                                                                                               >  1:1602438/1‑149 (MQ=255)
  tGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAg                                                                                                                                              >  1:1779766/1‑149 (MQ=255)
    aaCATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGga                                                                                                                                            >  2:1385791/1‑149 (MQ=255)
       aTGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGagaa                                                                                                                                         <  2:2878082/149‑1 (MQ=255)
        tGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGagaag                                                                                                                                        >  1:3793848/1‑149 (MQ=255)
        tGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGagaag                                                                                                                                        >  1:2706517/1‑149 (MQ=255)
          aaGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAgaagaa                                                                                                                                      >  1:746947/1‑149 (MQ=255)
          aaGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAgaagaa                                                                                                                                      <  1:3369398/149‑1 (MQ=255)
            ggTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAGGAATa                                                                                                                                    >  2:231183/1‑149 (MQ=255)
            ggTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATa                                                                                                                                    >  1:3578682/1‑149 (MQ=255)
              tGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATAcc                                                                                                                                  <  1:1905114/149‑1 (MQ=255)
               ggAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATAccc                                                                                                                                 <  1:412812/149‑1 (MQ=255)
                gAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCa                                                                                                                                <  1:1699074/149‑1 (MQ=255)
                 aaaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCAt                                                                                                                               <  2:2769744/149‑1 (MQ=255)
                  aaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATc                                                                                                                              <  1:2627853/149‑1 (MQ=255)
                     aCCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGc                                                                                                                           >  1:1064283/1‑149 (MQ=255)
                     aCCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGc                                                                                                                           >  1:1190544/1‑149 (MQ=255)
                      ccGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCa                                                                                                                          >  1:1804025/1‑149 (MQ=255)
                         ttGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATgg                                                                                                                       <  1:962233/149‑1 (MQ=255)
                            tCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCa                                                                                                                    >  1:197135/1‑149 (MQ=255)
                              aTTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCaaa                                                                                                                  <  2:1435537/149‑1 (MQ=255)
                              aTTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCaaa                                                                                                                  <  2:3754004/149‑1 (MQ=255)
                                  gTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAAtata                                                                                                              <  2:166263/149‑1 (MQ=255)
                                       gTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAatgat                                                                                                         <  2:2021936/149‑1 (MQ=255)
                                               aaTCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAg                                                                                                 <  1:3655047/149‑1 (MQ=255)
                                               aaTCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAg                                                                                                 <  1:2511453/149‑1 (MQ=255)
                                                 tctcTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCa                                                                                               >  2:3091462/1‑149 (MQ=255)
                                                  ctctATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCAt                                                                                              <  1:3513955/149‑1 (MQ=255)
                                                  ctctATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCAt                                                                                              >  2:3500728/1‑149 (MQ=255)
                                                     tATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAg                                                                                           >  1:589541/1‑149 (MQ=255)
                                                       tCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGt                                                                                         <  2:2708414/149‑1 (MQ=255)
                                                             gCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGAc                                                                                   >  2:379111/1‑149 (MQ=255)
                                                              cGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACt                                                                                  <  2:1267570/149‑1 (MQ=255)
                                                                aTGTTAAAAAATGTGATAAAATCTTGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCAATCTGCATGGGAAAATATAATGATGTTGTTAGCATCAGGTCAAGACttt                                                                                <  2:3665610/149‑1 (MQ=255)
                                                                aTGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACttt                                                                                >  1:836417/1‑149 (MQ=255)
                                                                  ggTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTta                                                                              <  2:475505/149‑1 (MQ=255)
                                                                  ggTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTta                                                                              <  2:1836518/149‑1 (MQ=255)
                                                                  ggTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTta                                                                              <  2:1793845/149‑1 (MQ=255)
                                                                    tAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGTATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtata                                                                            <  2:2172876/149‑1 (MQ=255)
                                                                     aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATaa                                                                           <  1:1416627/149‑1 (MQ=255)
                                                                     aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATaa                                                                           <  1:3672199/149‑1 (MQ=255)
                                                                     aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATaa                                                                           >  1:3732800/1‑149 (MQ=255)
                                                                     aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATaa                                                                           <  2:3578682/149‑1 (MQ=255)
                                                                         aaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaa                                                                       >  1:2812233/1‑149 (MQ=255)
                                                                         aaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaa                                                                       <  1:1553236/149‑1 (MQ=255)
                                                                          aGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaaa                                                                      >  1:420620/1‑149 (MQ=255)
                                                                           ggTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaaaa                                                                     >  1:466574/1‑149 (MQ=255)
                                                                           ggTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaaaa                                                                     >  1:3457179/1‑149 (MQ=255)
                                                                           ggTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaaaa                                                                     >  1:3218708/1‑149 (MQ=255)
                                                                           ggTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaaaa                                                                     >  1:2732708/1‑149 (MQ=255)
                                                                            gTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAAc                                                                    <  1:1256464/149‑1 (MQ=255)
                                                                               aTAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                 >  1:2778485/1‑149 (MQ=255)
                                                                               aTAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                 >  1:716715/1‑149 (MQ=255)
                                                                               aTAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                 >  1:3966210/1‑149 (MQ=255)
                                                                                  aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtat                                                              >  1:994168/1‑149 (MQ=255)
                                                                                  aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtat                                                              <  1:2350720/149‑1 (MQ=255)
                                                                                     tCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACt                                                           >  1:647329/1‑149 (MQ=255)
                                                                                         gCAACGCAGAGAATATGTTATGCATGTTGTCTGTAATAATGCACGACCAGGCCCAGATCCAGGAGAAGAACACCCATCTGCATGGGCAAATATAACGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCg                                                       <  1:489886/149‑1 (MQ=255)
                                                                                           aaaGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGATGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGgt                                                     >  1:2595133/1‑149 (MQ=255)
                                                                                            aaGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGgtg                                                    <  1:2186958/149‑1 (MQ=255)
                                                                                               cAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTaa                                                 <  2:949806/149‑1 (MQ=255)
                                                                                               cAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTaa                                                 <  2:1259568/149‑1 (MQ=255)
                                                                                               cAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTaa                                                 >  1:2902434/1‑149 (MQ=255)
                                                                                                aGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAAGACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAAc                                                <  1:1829308/149‑1 (MQ=255)
                                                                                                 gTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACt                                               >  2:4058147/1‑149 (MQ=255)
                                                                                                  tGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACtt                                              >  1:2419234/1‑149 (MQ=255)
                                                                                                  tGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACtt                                              >  2:1112802/1‑149 (MQ=255)
                                                                                                  tGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACtt                                              >  2:582924/1‑149 (MQ=255)
                                                                                                   taaTATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTta                                             <  2:3912321/148‑1 (MQ=255)
                                                                                                        ttttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATg                                        >  1:1109428/1‑149 (MQ=255)
                                                                                                         tttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgg                                       <  1:3112600/149‑1 (MQ=255)
                                                                                                         tttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgg                                       <  1:3329649/149‑1 (MQ=255)
                                                                                                           tATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgggg                                     <  2:3355428/149‑1 (MQ=255)
                                                                                                             tGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAACTATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                   <  2:661170/149‑1 (MQ=255)
                                                                                                             tGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                   >  1:2352190/1‑149 (MQ=255)
                                                                                                             tGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                   >  1:3400572/1‑149 (MQ=255)
                                                                                                             tGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                   >  1:3907626/1‑149 (MQ=255)
                                                                                                             tGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                   >  2:84510/1‑149 (MQ=255)
                                                                                                              gAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTg                                  <  1:1316695/149‑1 (MQ=255)
                                                                                                               aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                 >  2:2065048/1‑149 (MQ=255)
                                                                                                               aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                 >  2:3181107/1‑149 (MQ=255)
                                                                                                                  gTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTa                              <  2:1232620/149‑1 (MQ=255)
                                                                                                                        tGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTc                        <  1:79199/149‑1 (MQ=255)
                                                                                                                        tGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTc                        >  2:3421207/1‑149 (MQ=255)
                                                                                                                         gTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCt                       >  2:1481637/1‑149 (MQ=255)
                                                                                                                           aataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTAc                     >  2:3978556/1‑149 (MQ=255)
                                                                                                                           aataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTAc                     >  2:3411258/1‑149 (MQ=255)
                                                                                                                           aataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTATCGGTGTAACTTATAATGGGGTTGATTATGATTCTAc                     >  2:1547697/1‑149 (MQ=255)
                                                                                                                              aatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtc                  >  1:1097129/1‑149 (MQ=255)
                                                                                                                               atGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                 >  2:2754926/1‑149 (MQ=255)
                                                                                                                               atGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                 >  1:936271/1‑149 (MQ=255)
                                                                                                                                tGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtctc                <  2:3721799/149‑1 (MQ=255)
                                                                                                                                tGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtctc                <  2:1360114/149‑1 (MQ=255)
                                                                                                                                     tACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTa           <  2:3149145/149‑1 (MQ=255)
                                                                                                                                     tACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTa           >  1:3095247/1‑149 (MQ=255)
                                                                                                                                          ggCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTAt      >  2:2796529/1‑149 (MQ=255)
                                                                                                                                          ggCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTAt      >  2:1189298/1‑149 (MQ=255)
                                                                                                                                           gCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTAtt     >  2:4003471/1‑149 (MQ=255)
                                                                                                                                              cATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgca  >  1:3857247/1‑149 (MQ=255)
                                                                                                                                              cATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgca  <  2:1572895/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCA  >  CP009273/3184066‑3184356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑