Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,201,209 C→A 100% F160L (TTC→TTA)  ttdB → L‑tartrate dehydratase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,201,2090CA99.6% 716.5 / ‑4.3 221F160L (TTC→TTA) ttdBL‑tartrate dehydratase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (119/101);  total (119/102)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

GCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAC  >  CP009273/3201064‑3201354
                                                                                                                                                 |                                                                                                                                                 
gCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGc                                                                                                                                                >  2:1735386/1‑149 (MQ=255)
    ccAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGc                                                                                                                                            >  1:2783922/1‑149 (MQ=255)
    ccAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGc                                                                                                                                            <  2:844589/149‑1 (MQ=255)
      aGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTg                                                                                                                                          >  2:3373260/1‑149 (MQ=255)
       gAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGa                                                                                                                                         >  1:937781/1‑149 (MQ=255)
         aaTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGAtt                                                                                                                                       >  1:2170313/1‑149 (MQ=255)
         aaTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACACAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCGGGGTCAAAGAGTTAGGCCCGCTGAtt                                                                                                                                       <  2:73043/149‑1 (MQ=255)
           ttCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGt                                                                                                                                     >  2:3314430/1‑149 (MQ=255)
           ttCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGt                                                                                                                                     >  1:1396283/1‑149 (MQ=255)
           ttCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGt                                                                                                                                     >  2:590805/1‑149 (MQ=255)
              aaGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGtctc                                                                                                                                  <  1:3166639/149‑1 (MQ=255)
               aGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTctct                                                                                                                                 <  1:2944367/149‑1 (MQ=255)
                    cTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGa                                                                                                                            <  2:360676/149‑1 (MQ=255)
                     tACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGAt                                                                                                                           >  2:3904442/1‑149 (MQ=255)
                        aTGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATAcc                                                                                                                        >  2:2276910/1‑149 (MQ=255)
                          gtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                                                      <  1:2415215/149‑1 (MQ=255)
                          gtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                                                      >  1:2723391/1‑149 (MQ=255)
                           tgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCAc                                                                                                                     >  2:1096829/1‑149 (MQ=255)
                           tgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCAc                                                                                                                     >  1:1559328/1‑149 (MQ=255)
                           tgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCAc                                                                                                                     <  2:1839031/149‑1 (MQ=255)
                           tgATTGTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCAc                                                                                                                     <  1:984382/149‑1 (MQ=255)
                            gATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACg                                                                                                                    >  2:689128/1‑149 (MQ=255)
                            gATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACg                                                                                                                    <  2:746876/149‑1 (MQ=255)
                             aTTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACgg                                                                                                                   >  1:242189/1‑149 (MQ=255)
                              ttttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGc                                                                                                                  >  2:3056301/1‑149 (MQ=255)
                                ttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGcaa                                                                                                                <  2:240874/149‑1 (MQ=255)
                                ttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGcaa                                                                                                                <  2:1953770/149‑1 (MQ=255)
                                 tCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGcaac                                                                                                               >  2:640043/1‑149 (MQ=255)
                                      gCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCt                                                                                                          <  1:3016705/149‑1 (MQ=255)
                                      gCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCt                                                                                                          >  1:2810030/1‑149 (MQ=255)
                                        aGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGa                                                                                                        <  1:3939770/149‑1 (MQ=255)
                                        aGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGa                                                                                                        >  2:2475792/1‑149 (MQ=255)
                                         ggCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCTGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTTATACCCACGGCAACAACCTGAt                                                                                                       <  1:3601731/149‑1 (MQ=255)
                                         ggCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAt                                                                                                       >  1:3566354/1‑149 (MQ=255)
                                         ggCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACGGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAt                                                                                                       <  1:2804673/149‑1 (MQ=255)
                                          gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATa                                                                                                      <  2:268590/149‑1 (MQ=255)
                                          gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATa                                                                                                      <  2:273257/149‑1 (MQ=255)
                                          gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATa                                                                                                      >  2:2050246/1‑149 (MQ=255)
                                           cTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAg                                                                                                     <  2:1441900/149‑1 (MQ=255)
                                           cTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAg                                                                                                     <  2:3488027/149‑1 (MQ=255)
                                            tGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGc                                                                                                    >  2:1335318/1‑149 (MQ=255)
                                                  gTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaaa                                                                                              <  2:1190082/149‑1 (MQ=255)
                                                  gTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaaa                                                                                              >  1:654434/1‑149 (MQ=255)
                                                        gcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACaaaaa                                                                                        <  2:1189295/149‑1 (MQ=255)
                                                         cggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAg                                                                                       <  1:1841598/149‑1 (MQ=255)
                                                           gcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCt                                                                                     >  1:1144974/1‑149 (MQ=255)
                                                           gcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCt                                                                                     <  1:3707381/149‑1 (MQ=255)
                                                            cAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTg                                                                                    <  2:2865831/149‑1 (MQ=255)
                                                             aaCCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGt                                                                                   >  1:494487/1‑149 (MQ=255)
                                                               cccAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTc                                                                                 <  1:3205563/149‑1 (MQ=255)
                                                                 cAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGc                                                                               >  1:2027536/1‑149 (MQ=255)
                                                                         agagATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgcc                                                                       <  1:3409895/149‑1 (MQ=255)
                                                                         agagATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgcc                                                                       <  1:2276910/149‑1 (MQ=255)
                                                                         agagATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgcc                                                                       >  2:3837658/1‑149 (MQ=255)
                                                                          gagaTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                                      >  1:579648/1‑149 (MQ=255)
                                                                          gagaTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                                      >  1:3734499/1‑149 (MQ=255)
                                                                             aTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGa                                                                   >  2:1156927/1‑149 (MQ=255)
                                                                                  agaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                                                              <  2:1633037/149‑1 (MQ=255)
                                                                                  agaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                                                              >  2:922246/1‑149 (MQ=255)
                                                                                    aagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATc                                                            <  1:2475792/149‑1 (MQ=255)
                                                                                          aCTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                                                      >  1:1165843/1‑149 (MQ=255)
                                                                                          aCTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                                                      <  1:387499/149‑1 (MQ=255)
                                                                                           cTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAag                                                     <  1:1630069/149‑1 (MQ=255)
                                                                                            tGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAaga                                                    <  1:831139/149‑1 (MQ=255)
                                                                                            tGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAACCTGTTCGCCGAACGCCGCGATCCCATCGTGGAaga                                                    <  1:604991/149‑1 (MQ=255)
                                                                                             ggACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAagag                                                   <  1:667866/149‑1 (MQ=255)
                                                                                             ggACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAagag                                                   >  2:1280744/1‑149 (MQ=255)
                                                                                               aCAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                                                 >  1:233442/1‑149 (MQ=255)
                                                                                                 agagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                               >  2:3341223/1‑149 (MQ=255)
                                                                                                 agagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                               >  2:4062688/1‑149 (MQ=255)
                                                                                                 agagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                               <  2:3872123/149‑1 (MQ=255)
                                                                                                   agCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                                             <  1:518457/149‑1 (MQ=255)
                                                                                                   agCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                                             >  2:3101635/1‑149 (MQ=255)
                                                                                                    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTggg                                            >  2:3459911/1‑147 (MQ=255)
                                                                                                    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                                            >  1:2253057/1‑149 (MQ=255)
                                                                                                    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                                            <  2:2723391/149‑1 (MQ=255)
                                                                                                         gAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                       >  1:1897398/1‑149 (MQ=255)
                                                                                                         gAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                       <  1:505760/149‑1 (MQ=255)
                                                                                                         gAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                       >  1:146164/1‑149 (MQ=255)
                                                                                                            tGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTc                                    <  1:2748858/149‑1 (MQ=255)
                                                                                                                 gAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                               <  2:2352800/149‑1 (MQ=255)
                                                                                                                   gTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACa                             <  1:1960452/149‑1 (MQ=255)
                                                                                                                    tCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAt                            <  2:3670416/149‑1 (MQ=255)
                                                                                                                       cTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa                         >  2:502347/1‑149 (MQ=255)
                                                                                                                        tgtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa                        <  1:3119085/149‑1 (MQ=255)
                                                                                                                        tgtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa                        <  1:3447594/149‑1 (MQ=255)
                                                                                                                         gtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAt                       <  2:3259372/149‑1 (MQ=255)
                                                                                                                         gtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAt                       >  1:3219023/1‑149 (MQ=255)
                                                                                                                               cTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCt                 <  1:2718320/149‑1 (MQ=255)
                                                                                                                                       tCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGgaga         >  1:1761922/1‑149 (MQ=255)
                                                                                                                                       tCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGgaga         >  1:1286275/1‑149 (MQ=255)
                                                                                                                                        cAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGagag        <  2:242189/149‑1 (MQ=255)
                                                                                                                                          aaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGc      >  2:3380707/1‑149 (MQ=255)
                                                                                                                                          aaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGc      <  1:1735386/149‑1 (MQ=255)
                                                                                                                                          aaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGc      <  2:1896228/149‑1 (MQ=255)
                                                                                                                                          aaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGc      <  2:1484323/149‑1 (MQ=255)
                                                                                                                                             agTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCa   >  1:3145253/1‑149 (MQ=255)
                                                                                                                                             agTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCa   <  1:3581938/149‑1 (MQ=255)
                                                                                                                                             agTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCa   >  1:1022345/1‑149 (MQ=255)
                                                                                                                                              gTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAc  <  1:557494/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAC  >  CP009273/3201064‑3201354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑