| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,218,296 | G→T | 8.1% | D769Y (GAC→TAC) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,218,296 | 0 | G | T | 8.1% | 712.9 / 24.8 | 196 | D769Y (GAC→TAC) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (75/105); new base T (3/13); total (78/118) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 196 total aligned reads displayed.
GAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACT > CP009273/3218149‑3218443 | aaGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGAc < 2:3309702/149‑1 (MQ=255) aCGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAAc < 2:52051/149‑1 (MQ=255) gATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACca < 1:1132082/149‑1 (MQ=255) aTCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACcac > 2:3952042/1‑149 (MQ=255) cGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACaa > 2:1670933/1‑149 (MQ=255) cGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACaa < 2:1120312/149‑1 (MQ=255) tttGAGCTGCACCGTTCGCGGCTACAGCTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCACGCCGATGATCGACAACCACAAGc > 1:364604/1‑149 (MQ=255) tttGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGc > 1:364759/1‑149 (MQ=255) ttGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCTACAACCACAAGCa < 1:1672478/149‑1 (MQ=255) gAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAgg < 1:1038691/149‑1 (MQ=255) gCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAg > 1:3147831/1‑149 (MQ=255) ccGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCTACAACCACAAGCAGGAGTACGaa < 1:2985002/149‑1 (MQ=255) ccGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGaa < 1:537380/149‑1 (MQ=255) tCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGc < 1:3656920/149‑1 (MQ=255) aCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCaa > 1:3907582/1‑149 (MQ=255) cAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAc > 1:748098/1‑149 (MQ=255) aaCTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAGCCACAAGCAGGAGTACGAAGGGCTGTGGCAAcc > 1:3045039/1‑149 (MQ=255) aCTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCg > 1:825108/1‑149 (MQ=255) tCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAAt < 1:957762/149‑1 (MQ=255) cgcgATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATc > 1:2594171/1‑149 (MQ=255) cACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCTACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc < 2:2579527/149‑1 (MQ=255) cACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc < 1:1946305/149‑1 (MQ=255) aCCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCa < 2:564371/149‑1 (MQ=255) ccTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAg > 2:3824154/1‑149 (MQ=255) ttCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGAt < 1:2470269/149‑1 (MQ=255) ttCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGAt < 1:1608797/149‑1 (MQ=255) ctcAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAAGCATTTGCAGATCa > 2:3443490/1‑149 (MQ=255) tcAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCAt < 1:1610709/149‑1 (MQ=255) aaaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGc < 1:3580845/149‑1 (MQ=255) aaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCa < 2:1570320/149‑1 (MQ=255) aaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCa < 1:1002946/149‑1 (MQ=255) aaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCa < 1:1826689/149‑1 (MQ=255) aaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAg > 2:3921161/1‑149 (MQ=255) aaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAgg > 1:2654822/1‑149 (MQ=255) tGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGaa > 2:801504/1‑149 (MQ=255) gAGTGACAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAAc > 2:3117660/1‑149 (MQ=255) aGTGGCAAACCGACATCCTGGCAGGTGAATGGCGTATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACa < 2:3094118/149‑1 (MQ=255) aGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACa < 2:632229/149‑1 (MQ=255) aGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACa < 2:177568/149‑1 (MQ=255) tGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCTACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATc < 1:1333718/149‑1 (MQ=255) ggCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCt > 2:1477118/1‑149 (MQ=255) cAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTgc < 1:2673948/149‑1 (MQ=255) cAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTgc < 1:232949/149‑1 (MQ=255) ccGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGa < 1:1287955/149‑1 (MQ=255) cGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGAc < 1:1765857/149‑1 (MQ=255) gACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACt < 1:2654747/149‑1 (MQ=255) gACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACt < 1:2654698/149‑1 (MQ=255) aCATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACtt < 1:3610728/149‑1 (MQ=255) ntCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTg > 1:711661/2‑149 (MQ=255) tCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGc > 2:1934750/1‑149 (MQ=255) gCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAg > 1:1137971/1‑149 (MQ=255) aGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGaa > 2:449258/1‑149 (MQ=255) gTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACa > 1:3540874/1‑149 (MQ=255) gTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACa < 1:324849/149‑1 (MQ=255) gAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACaga < 1:425891/149‑1 (MQ=255) gAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACaga > 2:3814019/1‑149 (MQ=255) gAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACaga < 1:3129778/149‑1 (MQ=255) aaTGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACagag > 2:1325858/1‑149 (MQ=255) ggCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCTACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGa < 1:2064149/149‑1 (MQ=255) ggCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGa < 1:2523597/149‑1 (MQ=255) gCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGAt < 1:1532756/149‑1 (MQ=255) cGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATg < 1:506403/149‑1 (MQ=255) gAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATgg < 2:432297/149‑1 (MQ=255) aaTCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGt > 1:2237149/1‑149 (MQ=255) aaTCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGt < 1:2541415/149‑1 (MQ=255) aaTCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGt > 2:2516400/1‑149 (MQ=255) aaTCGCTGCTGACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGt > 2:2670975/1‑149 (MQ=255) aaTCGCTGCTGACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGt > 2:2670920/1‑149 (MQ=255) gctgctGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGTGCGACTTTGCCGTAGAACAGAGCGATGGTGAAg < 1:3033894/149‑1 (MQ=255) tgctgACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTg > 1:163450/1‑149 (MQ=255) gctgACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCTACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc < 1:785025/149‑1 (MQ=255) gctgACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc < 1:3932665/149‑1 (MQ=255) gctgACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc < 1:294612/149‑1 (MQ=255) gctgACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc < 2:2909167/149‑1 (MQ=255) gACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGa > 2:2340018/1‑149 (MQ=255) aCTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGat < 2:253583/149‑1 (MQ=255) cTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatc < 2:102334/149‑1 (MQ=255) tCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatca < 1:449258/149‑1 (MQ=255) cgcgAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatcat < 2:992060/149‑1 (MQ=255) gcgAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatcatc < 1:1182376/149‑1 (MQ=255) gcgAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatcatc < 2:3152760/149‑1 (MQ=255) gAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAg > 2:1621589/1‑149 (MQ=255) cAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGc > 2:904245/1‑149 (MQ=255) aaGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGTGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGcac > 2:3664447/1‑149 (MQ=255) aaGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGTGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGcac > 2:3670637/1‑149 (MQ=255) aGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGcaca > 1:2201253/1‑149 (MQ=255) aTCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGAACAGt > 2:3825829/1‑149 (MQ=255) aaCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAt < 1:3409841/149‑1 (MQ=255) aCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAtt > 1:2758510/1‑149 (MQ=255) aCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAtt < 1:1537320/149‑1 (MQ=255) aCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAtt > 2:671879/1‑149 (MQ=255) aCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAtt < 1:3893685/149‑1 (MQ=255) aCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAtt < 1:3548815/149‑1 (MQ=255) ttcttcAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGc < 2:1179763/149‑1 (MQ=255) tcttcAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGcc > 1:3776394/1‑149 (MQ=255) ttcAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGcccc < 1:1233873/149‑1 (MQ=255) cAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCccgc > 2:4089397/1‑149 (MQ=255) aaGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCccgcc > 1:4075582/1‑149 (MQ=255) gCCGATGATCTACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCgg < 1:643876/149‑1 (MQ=255) cGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACt < 2:1115209/149‑1 (MQ=255) | GAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACT > CP009273/3218149‑3218443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |