Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,245,848 G→T 100% L62L (CTG→CTT)  yhaH → DUF805 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,245,8480GT99.6% 858.8 / ‑4.4 263L62L (CTG→CTT) yhaHDUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (132/128);  total (133/130)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 264 total aligned reads displayed.

TTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245702‑3245993
                                                                                                                                                  |                                                                                                                                                 
 tCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTtgtg                                                                                                                                                >  1:3446831/1‑149 (MQ=255)
  ccGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTgtgt                                                                                                                                               <  2:2800516/149‑1 (MQ=255)
    ggggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttt                                                                                                                                             <  2:3018251/149‑1 (MQ=255)
     gggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGGTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCGGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtttt                                                                                                                                            >  1:3101044/1‑149 (MQ=255)
     gggTCGTGCGCGGCGAAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtttt                                                                                                                                            >  1:2983043/1‑149 (MQ=255)
      ggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttttt                                                                                                                                           >  2:351438/1‑149 (MQ=255)
      ggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttttt                                                                                                                                           <  2:1231656/149‑1 (MQ=255)
      ggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttttt                                                                                                                                           <  2:328580/149‑1 (MQ=255)
       gTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTa                                                                                                                                          >  1:2368977/1‑149 (MQ=255)
       gTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTa                                                                                                                                          <  1:2163816/149‑1 (MQ=255)
        tCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTAc                                                                                                                                         <  2:2029550/149‑1 (MQ=255)
                ggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTGGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAAGGCGCTGGTGGCGAAGGCTTCCTGACGACAATCTTCGGTATTCTTGTGTTTTTACCgtggtgt                                                                                                                                 <  1:3606137/149‑2 (MQ=255)
                 gcgcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTggg                                                                                                                                >  2:2574864/1‑149 (MQ=255)
                  cgcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGc                                                                                                                               <  1:2892954/149‑1 (MQ=255)
                  cgcAAAGAGTACTGGATGTTTAGTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGc                                                                                                                               <  1:2802602/149‑1 (MQ=255)
                   gcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCg                                                                                                                              <  2:40938/149‑1 (MQ=255)
                   gcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCg                                                                                                                              <  2:40690/149‑1 (MQ=255)
                     aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGt                                                                                                                            <  2:1988022/149‑1 (MQ=255)
                     aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGt                                                                                                                            >  1:1154131/1‑149 (MQ=255)
                      aaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           >  1:1452617/1‑149 (MQ=255)
                       agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTc                                                                                                                          >  2:672949/1‑149 (MQ=255)
                       agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTc                                                                                                                          <  2:1567132/149‑1 (MQ=255)
                          gTACTGGATGTTTATTCTGGTCAACATCATCTTTACTTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                                                       >  1:165900/1‑149 (MQ=255)
                            aCTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTc                                                                                                                     <  1:3153528/149‑1 (MQ=255)
                              tGGATGTTTATTCTTGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccg                                                                                                                   >  1:2305310/1‑149 (MQ=255)
                               ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgc                                                                                                                  >  2:1432588/1‑149 (MQ=255)
                                 aTGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgccg                                                                                                                <  2:236102/149‑1 (MQ=255)
                                 aTGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgccg                                                                                                                >  1:3187435/1‑149 (MQ=255)
                                       aTTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTCGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCa                                                                                                          <  2:881797/149‑1 (MQ=255)
                                           tGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGac                                                                                                      <  2:2625607/149‑1 (MQ=255)
                                            ggTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGaca                                                                                                     >  1:3336115/1‑149 (MQ=255)
                                             gTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGacac                                                                                                    >  2:886469/1‑149 (MQ=255)
                                              tCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACacc                                                                                                   >  2:3015118/1‑149 (MQ=255)
                                               cAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCGGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccg                                                                                                  <  2:3397986/149‑1 (MQ=255)
                                                aaCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccga                                                                                                 >  2:3441284/1‑149 (MQ=255)
                                                 aCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgac                                                                                                >  1:2540089/1‑149 (MQ=255)
                                                   atcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgaccg                                                                                              >  2:2671286/1‑149 (MQ=255)
                                                    tcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGc                                                                                             >  2:3658690/1‑149 (MQ=255)
                                                             cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtgg                                                                                    <  2:2030868/149‑1 (MQ=255)
                                                              gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                   >  1:367972/1‑149 (MQ=255)
                                                              gTTCGTGCTGGGGCTGCTGGATAAAATGTGAGGCGGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                   <  1:4076138/149‑1 (MQ=255)
                                                               ttCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTTCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:1935692/1‑149 (MQ=255)
                                                               ttCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:720438/1‑149 (MQ=255)
                                                               ttCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:1420636/1‑149 (MQ=255)
                                                                 cGTGCGGGGGCTGCTGGATAAAATGTTCGGCTGGCACCGCGCGGGGGGCGACGGCATCCTGACGACAATCTCCGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCTGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTggg                                                                                <  1:1742566/149‑1 (MQ=255)
                                                                   tGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                                              >  1:3946467/1‑149 (MQ=255)
                                                                     cTGGGGCTGCTGGATAAAATGTTCGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                                            <  2:2286034/149‑1 (MQ=255)
                                                                     cTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                                            <  2:211388/149‑1 (MQ=255)
                                                                      gggggCTGCGGGATAAAATGTGAGGCTGGCCACGCGCGGGGGGCGAAGCCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTCCCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTg                                                                           <  1:2707271/148‑1 (MQ=255)
                                                                       ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGc                                                                          >  2:3160460/1‑149 (MQ=255)
                                                                       ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGc                                                                          >  1:582578/1‑149 (MQ=255)
                                                                        gggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCt                                                                         <  1:2451807/149‑1 (MQ=255)
                                                                        gggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCt                                                                         >  1:3258321/1‑149 (MQ=255)
                                                                         ggCTGCGGGATAAAATGTGAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTGTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTa                                                                        <  1:2149888/149‑1 (MQ=255)
                                                                          gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                                       >  1:1241391/1‑149 (MQ=255)
                                                                           ctgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                                      <  2:2998286/149‑1 (MQ=255)
                                                                           ctgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGGGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                                      <  1:3780849/149‑1 (MQ=255)
                                                                            tgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTc                                                                     >  1:1035359/1‑149 (MQ=255)
                                                                            tgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTc                                                                     >  1:1815168/1‑149 (MQ=255)
                                                                            tgctcGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGTTATTCTTGTGTTTTTACATTTGTGGGCGGTTCAGATCCGCCCCCTGCACGAAACCGACAGCTCGGCGTGGTGGGCACTGCTATTc                                                                     >  2:3591896/1‑149 (MQ=255)
                                                                              ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCtt                                                                   >  2:2202426/1‑149 (MQ=255)
                                                                                ggATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTaa                                                                 >  2:2316547/1‑149 (MQ=255)
                                                                                    aaaaTGTTAGGCTGGCACCGCGCGGGGGGCTAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATccc                                                             <  1:3839440/149‑1 (MQ=255)
                                                                                     aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  2:1704697/149‑1 (MQ=255)
                                                                                     aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            >  1:1851409/1‑149 (MQ=255)
                                                                                        tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                                         >  1:1887975/1‑149 (MQ=255)
                                                                                        tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                                         >  1:775745/1‑149 (MQ=255)
                                                                                         gTTAGGCTGGCACCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTa                                                        <  1:2061424/149‑1 (MQ=255)
                                                                                         gTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTa                                                        <  2:1035359/149‑1 (MQ=255)
                                                                                             ggCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCgg                                                    >  1:641190/1‑149 (MQ=255)
                                                                                                  gCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctggc                                               <  2:2449202/149‑1 (MQ=255)
                                                                                                   cAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctggct                                              >  2:3706872/1‑149 (MQ=255)
                                                                                                    aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtt                                             >  2:4004617/1‑149 (MQ=255)
                                                                                                    aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtt                                             >  2:4005463/1‑149 (MQ=255)
                                                                                                     aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                                            <  2:2545399/149‑1 (MQ=255)
                                                                                                     aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                                            <  2:2982805/149‑1 (MQ=255)
                                                                                                     aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                                            <  1:2848772/149‑1 (MQ=255)
                                                                                                      cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                                           <  2:1154131/149‑1 (MQ=255)
                                                                                                      cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                                           >  1:2251447/1‑149 (MQ=255)
                                                                                                        cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatta                                         <  1:3014106/149‑1 (MQ=255)
                                                                                                          cTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatc                                       <  2:185957/149‑1 (MQ=255)
                                                                                                                gCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTc                                 <  2:4098567/149‑1 (MQ=255)
                                                                                                                 cGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCt                                <  1:716976/149‑1 (MQ=255)
                                                                                                                  gAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCtt                               <  1:3660921/149‑1 (MQ=255)
                                                                                                                     ggCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa                            >  2:1290361/1‑149 (MQ=255)
                                                                                                                     ggCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACAGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTTGGCACTGCTATACTTAATCCAGTTTATCGCCTGGCTTATTATCATCGTCTATaa                            >  2:3686484/1‑149 (MQ=255)
                                                                                                                         tCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGc                        >  2:1191067/1‑149 (MQ=255)
                                                                                                                          ccTGACGACAATCTAGGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCTGTTGCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGcc                       <  1:1007975/149‑1 (MQ=255)
                                                                                                                            tGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg                     <  2:94147/149‑1 (MQ=255)
                                                                                                                            tGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg                     >  1:1408231/1‑149 (MQ=255)
                                                                                                                               cgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCg                  <  2:829078/149‑1 (MQ=255)
                                                                                                                                gacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCgg                 >  2:2926788/1‑149 (MQ=255)
                                                                                                                                   aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTa              <  2:2623337/149‑1 (MQ=255)
                                                                                                                                   aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTa              >  2:1281871/1‑149 (MQ=255)
                                                                                                                                    aTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             >  1:2589574/1‑149 (MQ=255)
                                                                                                                                      cTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGc           <  1:1359015/149‑1 (MQ=255)
                                                                                                                                            tATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATAGTCTTTAACTGCCAGGCGGGTACGCCAGGCg     >  1:3821516/1‑149 (MQ=255)
                                                                                                                                              ttCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGaa   >  2:2806571/1‑149 (MQ=255)
                                                                                                                                              ttCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGaa   >  2:56543/1‑149 (MQ=255)
                                                                                                                                              ttCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGaa   >  1:3651962/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245702‑3245993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑