Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 282,483 C→A 6.3% H460N (CAT→AAT)  yagG → CP4‑6 prophage; putative sugar transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273282,4830CA 6.3% 746.5 / 15.7 198H460N (CAT→AAT) yagGCP4‑6 prophage; putative sugar transporter
Reads supporting (aligned to +/- strand):  ref base C (83/102);  new base A (6/7);  total (89/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

GGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCGTGA  >  CP009273/282335‑282630
                                                                                                                                                    |                                                                                                                                                   
ggtgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATccc                                                                                                                                                     <  1:2262834/149‑1 (MQ=255)
 gtgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCa                                                                                                                                                    <  1:952823/149‑1 (MQ=255)
 gtgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCa                                                                                                                                                    <  1:2089053/149‑1 (MQ=255)
 gtgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCa                                                                                                                                                    <  1:2089005/149‑1 (MQ=255)
  tgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCAt                                                                                                                                                   <  2:2054721/149‑1 (MQ=255)
     cGGTGGTTCTCTACGCGGTCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTaa                                                                                                                                                <  2:3029288/149‑1 (MQ=255)
          gtgCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGa                                                                                                                                           <  2:1627799/149‑1 (MQ=255)
             ctctACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGc                                                                                                                                        <  1:1908059/149‑1 (MQ=255)
                 aCGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAata                                                                                                                                    <  2:2716767/149‑1 (MQ=255)
                 aCGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAata                                                                                                                                    <  2:2602681/149‑1 (MQ=255)
                  cgcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAatat                                                                                                                                   <  1:374910/149‑1 (MQ=255)
                  cgcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAatat                                                                                                                                   <  1:2125635/149‑1 (MQ=255)
                   gcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATg                                                                                                                                  <  1:3645951/149‑1 (MQ=255)
                   gcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATg                                                                                                                                  <  1:510218/149‑1 (MQ=255)
                     gggCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGa                                                                                                                                <  1:2211231/149‑1 (MQ=255)
                     gggCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGa                                                                                                                                <  2:401897/149‑1 (MQ=255)
                        cATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAAt                                                                                                                             <  1:3482391/149‑1 (MQ=255)
                            ttCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCACTATGGAAATCACt                                                                                                                         >  2:4051258/1‑149 (MQ=255)
                             tcatcaTGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                                                        <  1:4065667/149‑1 (MQ=255)
                                tcatGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCACTATGGAACTCACTAAcc                                                                                                                     >  2:873779/1‑149 (MQ=255)
                                tcatGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAAcc                                                                                                                     >  1:3803661/1‑149 (MQ=255)
                                 catGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAAccc                                                                                                                    >  1:1131498/1‑149 (MQ=255)
                                 catGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAAccc                                                                                                                    <  1:3595767/149‑1 (MQ=255)
                                  atGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCg                                                                                                                   >  2:1493651/1‑149 (MQ=255)
                                   tGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGa                                                                                                                  >  1:2821592/1‑149 (MQ=255)
                                   tGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGa                                                                                                                  >  2:2412814/1‑149 (MQ=255)
                                        tCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTc                                                                                                             <  1:1611923/149‑1 (MQ=255)
                                        tCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTc                                                                                                             <  2:4030073/149‑1 (MQ=255)
                                        tCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTc                                                                                                             <  1:1612889/149‑1 (MQ=255)
                                        tCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTc                                                                                                             >  1:3740175/1‑149 (MQ=255)
                                         cGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCa                                                                                                            <  2:703784/149‑1 (MQ=255)
                                          gCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCAc                                                                                                           <  2:3451945/149‑1 (MQ=255)
                                          gCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCAc                                                                                                           <  1:1914499/149‑1 (MQ=255)
                                          gCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCAc                                                                                                           >  2:1567404/1‑149 (MQ=255)
                                           ctctACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCAcc                                                                                                          >  1:85085/1‑149 (MQ=255)
                                            tctACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCg                                                                                                         >  2:2229782/1‑149 (MQ=255)
                                              tACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGc                                                                                                       >  1:856916/1‑149 (MQ=255)
                                                 aaGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTc                                                                                                    <  1:3316163/149‑1 (MQ=255)
                                                 aaGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTc                                                                                                    >  1:3257554/1‑149 (MQ=255)
                                                   gCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCaa                                                                                                  <  2:1748161/149‑1 (MQ=255)
                                                     tCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAAcc                                                                                                >  1:3932839/1‑149 (MQ=255)
                                                     tCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAAcc                                                                                                >  1:2950355/1‑149 (MQ=255)
                                                      cACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAAccc                                                                                               <  2:1028947/149‑1 (MQ=255)
                                                           aTGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGAcc                                                                                          >  1:2989689/1‑149 (MQ=255)
                                                           aTGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGAcc                                                                                          >  1:657480/1‑149 (MQ=255)
                                                           aTGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGACGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGTCTTAAACCCGGAcc                                                                                          >  2:2706146/1‑149 (MQ=255)
                                                            tGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAACTCACTAACCCGATACTCACCGGCTTCAACCCGGAccc                                                                                         >  1:3589669/1‑149 (MQ=255)
                                                            tGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGAccc                                                                                         >  1:914723/1‑149 (MQ=255)
                                                                cgcgTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTcc                                                                                     <  2:3457210/149‑1 (MQ=255)
                                                                 gcgTGGAGGCCAGCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTccc                                                                                    >  2:3349121/1‑149 (MQ=255)
                                                                     ggAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGACGCAATCTGGAAATCACTAACCCGATACTCACCGCCTTCAACCCGGACCCGTCCCtgtg                                                                                >  1:2625690/1‑149 (MQ=255)
                                                                         gCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGATCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCACTATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTgccgc                                                                            >  2:386479/1‑149 (MQ=255)
                                                                            aTCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAg                                                                         >  1:1602863/1‑149 (MQ=255)
                                                                                gCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCg                                                                     >  1:416811/1‑149 (MQ=255)
                                                                                  cGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAg                                                                   <  1:1893688/149‑1 (MQ=255)
                                                                                  cGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAg                                                                   <  2:570528/149‑1 (MQ=255)
                                                                                   ggCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAgg                                                                  >  1:814467/1‑149 (MQ=255)
                                                                                     cAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGac                                                                >  1:722194/1‑149 (MQ=255)
                                                                                     cAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGac                                                                >  1:1436710/1‑149 (MQ=255)
                                                                                     cAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGac                                                                <  1:1479336/149‑1 (MQ=255)
                                                                                     cAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGac                                                                <  1:2826737/149‑1 (MQ=255)
                                                                                       gCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGacta                                                              >  1:3854763/1‑149 (MQ=255)
                                                                                        cTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGactac                                                             >  2:2451921/1‑149 (MQ=255)
                                                                                        cTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGactac                                                             <  2:2409409/149‑1 (MQ=255)
                                                                                        cTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGactac                                                             <  2:874304/149‑1 (MQ=255)
                                                                                         tGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGactact                                                            <  2:1790931/149‑1 (MQ=255)
                                                                                          gATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCACTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGActacta                                                           >  2:309438/1‑149 (MQ=255)
                                                                                                gCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGc                                                     >  1:1379139/1‑149 (MQ=255)
                                                                                                  aCCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCa                                                   >  2:948865/1‑149 (MQ=255)
                                                                                                  aCCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCa                                                   <  2:2918874/149‑1 (MQ=255)
                                                                                                   ccGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCAc                                                  <  1:2412814/149‑1 (MQ=255)
                                                                                                   ccGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCAc                                                  >  1:2478036/1‑149 (MQ=255)
                                                                                                   ccGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCAc                                                  >  2:3067862/1‑149 (MQ=255)
                                                                                                        cggcgCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGa                                             <  2:1143756/149‑1 (MQ=255)
                                                                                                         ggcgCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGAc                                            >  2:4062741/1‑149 (MQ=255)
                                                                                                          gcgcAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGAcc                                           >  1:523023/1‑149 (MQ=255)
                                                                                                           cgcAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCt                                          <  2:3766452/149‑1 (MQ=255)
                                                                                                            gcAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCtt                                         <  1:1209478/149‑1 (MQ=255)
                                                                                                              aGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCg                                       >  2:206299/1‑149 (MQ=255)
                                                                                                              aGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCg                                       >  2:60129/1‑149 (MQ=255)
                                                                                                               gggCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGa                                      >  1:1125453/1‑149 (MQ=255)
                                                                                                                 gCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGt                                    >  1:768674/1‑149 (MQ=255)
                                                                                                                   gAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTgg                                  >  2:3183326/1‑149 (MQ=255)
                                                                                                                    aGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGt                                 >  1:1971079/1‑149 (MQ=255)
                                                                                                                    aGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGt                                 >  1:750459/1‑149 (MQ=255)
                                                                                                                     ggCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGtt                                <  2:1991518/149‑1 (MQ=255)
                                                                                                                     ggCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGtt                                <  2:1684836/149‑1 (MQ=255)
                                                                                                                       ccGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTcc                              <  1:2883018/149‑1 (MQ=255)
                                                                                                                        cGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTccc                             <  1:3075889/149‑1 (MQ=255)
                                                                                                                         gTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCg                            >  1:2703390/1‑149 (MQ=255)
                                                                                                                                     gcgACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTTTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATc                <  2:1632374/149‑1 (MQ=255)
                                                                                                                                           gCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCAc          <  2:3471421/149‑1 (MQ=255)
                                                                                                                                           gCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCAc          <  2:85085/149‑1 (MQ=255)
                                                                                                                                              gCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTcc       >  2:2468505/1‑149 (MQ=255)
                                                                                                                                              gCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTcc       >  2:2233673/1‑149 (MQ=255)
                                                                                                                                                aTCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCg     <  1:3081315/149‑1 (MQ=255)
                                                                                                                                                aTCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCg     <  2:2521410/149‑1 (MQ=255)
                                                                                                                                                aTCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGCGCGTGCGCATCTACCACTCCCg     >  1:3428914/1‑149 (MQ=255)
                                                                                                                                                aTCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCg     >  1:185632/1‑149 (MQ=255)
                                                                                                                                                   ccACTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCGTGa  <  1:309438/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCGTGA  >  CP009273/282335‑282630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑