Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,254,752 C→A 100% V342V (GTG→GTT)  tdcE ← pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,254,7520CA99.5% 718.4 / ‑4.3 220V342V (GTG→GTT) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (107/112);  total (107/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

CATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGC  >  CP009273/3254604‑3254897
                                                                                                                                                    |                                                                                                                                                 
cATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATc                                                                                                                                                   <  2:3276167/149‑1 (MQ=255)
     tttttGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTc                                                                                                                                              <  2:829952/149‑1 (MQ=255)
       tttGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCg                                                                                                                                            <  1:2811443/149‑1 (MQ=255)
         tGAAGACAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTc                                                                                                                                          <  1:2776523/149‑1 (MQ=255)
         tGAAGACAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTc                                                                                                                                          <  1:2776753/149‑1 (MQ=255)
            aGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGcc                                                                                                                                       >  2:3338171/1‑149 (MQ=255)
             ggCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGccc                                                                                                                                      >  1:2193253/1‑149 (MQ=255)
             ggCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGccc                                                                                                                                      <  1:212843/149‑1 (MQ=255)
              gCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCa                                                                                                                                     >  1:2424296/1‑149 (MQ=255)
               cAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAg                                                                                                                                    >  2:2511121/1‑149 (MQ=255)
               cAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAg                                                                                                                                    >  1:480698/1‑149 (MQ=255)
                aaTCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGa                                                                                                                                   >  2:2670332/1‑149 (MQ=255)
                 aTCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGAt                                                                                                                                  >  1:3940116/1‑149 (MQ=255)
                  tCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGAtt                                                                                                                                 >  2:2706086/1‑149 (MQ=255)
                   cGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTg                                                                                                                                <  2:1158058/149‑1 (MQ=255)
                   cGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTg                                                                                                                                >  2:3565282/1‑149 (MQ=255)
                   cGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTg                                                                                                                                >  2:3565548/1‑149 (MQ=255)
                       aaTTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTc                                                                                                                            <  1:166542/149‑1 (MQ=255)
                         ttcttcCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGc                                                                                                                          <  1:175095/149‑1 (MQ=255)
                          tcttcCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGcc                                                                                                                         <  2:2996119/149‑1 (MQ=255)
                           cttcCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCg                                                                                                                        >  2:2804250/1‑149 (MQ=255)
                                gACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGaa                                                                                                                   >  1:2288582/1‑149 (MQ=255)
                                gACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGaa                                                                                                                   >  2:2158773/1‑149 (MQ=255)
                                    aaaGAATGGTCAGGTTAGGTTGCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCATATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGc                                                                                                               <  2:136138/149‑1 (MQ=255)
                                     aaGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCg                                                                                                              <  2:3180804/149‑1 (MQ=255)
                                       gAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAAGAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGaa                                                                                                            <  2:3092726/149‑1 (MQ=255)
                                             tCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAAt                                                                                                      <  1:1941289/149‑1 (MQ=255)
                                               aggttaggttCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTc                                                                                                    <  1:2023972/149‑1 (MQ=255)
                                                ggttaggttCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTcc                                                                                                   >  1:490480/1‑149 (MQ=255)
                                                   taggttCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGgt                                                                                                >  1:437254/1‑149 (MQ=255)
                                                       ttCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTAc                                                                                            >  1:1064187/1‑149 (MQ=255)
                                                       ttCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTAc                                                                                            >  2:2847875/1‑149 (MQ=255)
                                                            gTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAgg                                                                                       >  2:1179150/1‑149 (MQ=255)
                                                                 ggCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                  <  2:1849081/149‑1 (MQ=255)
                                                                 ggCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                  <  1:2511121/149‑1 (MQ=255)
                                                                 ggCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                  >  1:1514505/1‑149 (MQ=255)
                                                                  gCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCTGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGt                                                                                 >  1:1282527/1‑149 (MQ=255)
                                                                  gCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGt                                                                                 >  1:3333491/1‑149 (MQ=255)
                                                                    cccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAc                                                                               >  2:895531/1‑149 (MQ=255)
                                                                     ccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAcc                                                                              >  2:750888/1‑149 (MQ=255)
                                                                     ccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAcc                                                                              <  2:2142591/149‑1 (MQ=255)
                                                                        tAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGACGCGTACCATa                                                                           >  2:2771223/1‑149 (MQ=255)
                                                                        tAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATa                                                                           >  2:2771252/1‑149 (MQ=255)
                                                                        tAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATa                                                                           >  2:3302436/1‑149 (MQ=255)
                                                                        tAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATa                                                                           <  2:1592275/149‑1 (MQ=255)
                                                                             tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGAt                                                                      >  2:2987627/1‑149 (MQ=255)
                                                                             tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGAt                                                                      >  1:2153255/1‑149 (MQ=255)
                                                                             tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGAt                                                                      >  1:75584/1‑149 (MQ=255)
                                                                                 gggTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTc                                                                  <  1:1366701/149‑1 (MQ=255)
                                                                                    tgtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatg                                                               <  2:2013803/149‑1 (MQ=255)
                                                                                     gtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatga                                                              <  1:670312/149‑1 (MQ=255)
                                                                                       gCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatgatg                                                            <  1:2170293/149‑1 (MQ=255)
                                                                                          aaTAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAg                                                         >  2:2922574/1‑149 (MQ=255)
                                                                                           aTAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGt                                                        >  2:3889000/1‑149 (MQ=255)
                                                                                           aTAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGt                                                        <  2:3994619/149‑1 (MQ=255)
                                                                                             aGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTga                                                      <  1:873397/149‑1 (MQ=255)
                                                                                              gCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgat                                                     <  1:3412164/149‑1 (MQ=255)
                                                                                              gCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgat                                                     >  1:1603821/1‑149 (MQ=255)
                                                                                               cGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatc                                                    <  1:948259/149‑1 (MQ=255)
                                                                                               cGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatc                                                    <  1:1005444/149‑1 (MQ=255)
                                                                                                ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcg                                                   >  1:1383590/1‑149 (MQ=255)
                                                                                                ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcg                                                   >  1:1363157/1‑149 (MQ=255)
                                                                                                 gAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcga                                                  <  1:2597070/149‑1 (MQ=255)
                                                                                                  aaGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgat                                                 <  2:813897/149‑1 (MQ=255)
                                                                                                  aaGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgat                                                 <  2:2291822/149‑1 (MQ=255)
                                                                                                   aGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAGGTgatcgatc                                                >  2:4026903/1‑149 (MQ=255)
                                                                                                   aGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgatc                                                <  1:2965976/149‑1 (MQ=255)
                                                                                                   aGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgatc                                                <  1:2648851/149‑1 (MQ=255)
                                                                                                         ttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTcc                                          <  1:2313786/149‑1 (MQ=255)
                                                                                                         ttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTcc                                          >  1:1713667/1‑149 (MQ=255)
                                                                                                             ggTCACCAGCGTACGACCGTCCAGCCCCATCACGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGAGTACGCAGGAAGCGTACCATAAGGATCTTCATGATGAAGTGATCGATCAGATCCagtt                                      >  2:1010128/1‑148 (MQ=37)
                                                                                                                  ccAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgc                                 >  2:540167/1‑149 (MQ=255)
                                                                                                                  ccAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgc                                 >  2:2966606/1‑149 (MQ=255)
                                                                                                                  ccAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgc                                 <  2:3477639/149‑1 (MQ=255)
                                                                                                                   cAGCGTACGACCGTCCAGTCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgct                                <  1:1409334/149‑1 (MQ=255)
                                                                                                                   cAGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgct                                <  1:2342188/149‑1 (MQ=255)
                                                                                                                    aGCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgctg                               >  1:3965895/1‑149 (MQ=255)
                                                                                                                     gCGTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAAt                                                                                                      >  1:3439015/1‑77 (MQ=255)
                                                                                                                       gTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTc                            >  1:1484202/1‑149 (MQ=255)
                                                                                                                       gTACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTc                            <  2:2274989/149‑1 (MQ=255)
                                                                                                                        tACGACCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCa                           >  2:1805574/1‑149 (MQ=255)
                                                                                                                            aCCGTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGa                       >  1:3149208/1‑149 (MQ=255)
                                                                                                                               gTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTa                    >  1:3633724/1‑149 (MQ=255)
                                                                                                                               gTCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTa                    <  2:437254/149‑1 (MQ=255)
                                                                                                                                tCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTAc                   >  1:2705149/1‑149 (MQ=255)
                                                                                                                                tCCAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTAc                   >  1:613535/1‑149 (MQ=255)
                                                                                                                                  cAGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGc                 >  1:2562558/1‑149 (MQ=255)
                                                                                                                                   aGCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGcc                <  2:2424296/149‑1 (MQ=255)
                                                                                                                                    gCCCCATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCa               >  2:3761265/1‑149 (MQ=255)
                                                                                                                                      cccATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGc             >  1:2663774/1‑149 (MQ=255)
                                                                                                                                      cccATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGc             >  1:597133/1‑149 (MQ=255)
                                                                                                                                      cccATCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGc             <  1:3023296/149‑1 (MQ=255)
                                                                                                                                         aTCCCGCCGATAACGTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCttt          <  2:2193253/149‑1 (MQ=255)
                                                                                                                                          tCCCGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTa         <  2:3148036/149‑1 (MQ=255)
                                                                                                                                           cccGCCGATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTaa        <  2:2317087/149‑1 (MQ=255)
                                                                                                                                             cgccgATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAg      <  1:2352895/149‑1 (MQ=255)
                                                                                                                                              gccgATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGt     <  2:2846337/149‑1 (MQ=255)
                                                                                                                                              gccgATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGt     <  1:2721248/149‑1 (MQ=255)
                                                                                                                                              gccgATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGt     <  1:2721289/149‑1 (MQ=255)
                                                                                                                                                 gATAACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTcgc  >  1:3843408/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CATATTTTTTGAAGGCAATCGGTAATTCTTCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGC  >  CP009273/3254604‑3254897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑