BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,268,357	C→A	100%	intergenic (‑91/‑284)	garP ← / → garD	putative (D)‑galactarate transporter/(D)‑galactarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,268,357	0	C	A	99.6%	740.8 / ‑4.4	222	intergenic (‑91/‑284)	garP/garD	putative (D)‑galactarate transporter/(D)‑galactarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (113/108); total (114/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 222 total aligned reads displayed.

ATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACT  >  CP009273/3268211‑3268501

atTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAgg                                                                                                                                                <  2:2230897/149‑1 (MQ=255)

   aaaTAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGaa                                                                                                                                             >  2:3243877/1‑149 (MQ=255)

    aaTAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAAt                                                                                                                                            >  1:836243/1‑149 (MQ=255)

     aTAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAAtt                                                                                                                                           >  1:1424569/1‑149 (MQ=255)

       aGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAg                                                                                                                                         <  1:3168492/149‑1 (MQ=255)

       aGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAg                                                                                                                                         >  1:2340799/1‑149 (MQ=255)

        gCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGt                                                                                                                                        >  1:3872135/1‑149 (MQ=255)

        gCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGt                                                                                                                                        <  2:216035/149‑1 (MQ=255)

         cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGta                                                                                                                                       <  1:1697674/149‑1 (MQ=255)

         cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGta                                                                                                                                       <  1:2132192/149‑1 (MQ=255)

              aTGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAagc                                                                                                                                  >  1:3649283/1‑149 (MQ=255)

              aTGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAagc                                                                                                                                  >  1:1603470/1‑149 (MQ=255)

               tGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAagca                                                                                                                                 <  2:2511299/149‑1 (MQ=255)

               tGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAagca                                                                                                                                 >  1:2110083/1‑149 (MQ=255)

               tGCACGCCTTTCTTTTTTTCGTCAACGGTGTCAAGAATCATTTTATTTACCTCGTGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAagca                                                                                                                                 <  2:2609354/149‑1 (MQ=255)

                gCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAagcag                                                                                                                                <  1:2963722/149‑1 (MQ=255)

                    gCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGcgcg                                                                                                                            <  2:3657060/149‑1 (MQ=255)

                     ccTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCga                                                                                                                           >  2:3993419/1‑149 (MQ=255)

                      ctttctttTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCgag                                                                                                                          >  1:516229/1‑149 (MQ=255)

                           tttttttCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGaataa                                                                                                                     >  1:1550470/1‑149 (MQ=255)

                              ttttCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGaataataa                                                                                                                  <  1:1056276/149‑1 (MQ=255)

                                ttCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATc                                                                                                                <  1:728101/149‑1 (MQ=255)

                                ttCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATc                                                                                                                <  2:1328964/149‑1 (MQ=255)

                                   gTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCAtt                                                                                                             <  1:2625430/149‑1 (MQ=255)

                                       aCGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGc                                                                                                         >  2:206631/1‑149 (MQ=255)

                                         ggTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCaa                                                                                                       <  2:1519501/149‑1 (MQ=255)

                                          gtgtCCAGAATCATTTTATTTACCTCGTGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCaaa                                                                                                      <  2:1923995/149‑1 (MQ=255)

                                           tgtCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAAt                                                                                                     <  2:3929449/149‑1 (MQ=255)

                                           tgtCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAAt                                                                                                     <  1:2579822/149‑1 (MQ=255)

                                               cAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCta                                                                                                 <  1:1953117/149‑1 (MQ=255)

                                                 gAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCtaat                                                                                               <  1:2836913/149‑1 (MQ=255)

                                                   aTCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCtaattt                                                                                             <  2:2642016/149‑1 (MQ=255)

                                                     cATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCtaatttaa                                                                                           <  2:1891956/149‑1 (MQ=255)

                                                       ttttATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCtaatttaatt                                                                                         <  2:80679/149‑1 (MQ=255)

                                                         ttatttaCCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATttaattaa                                                                                       <  1:3949349/149‑1 (MQ=255)

                                                          tatttaCCTCTGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTtaattaat                                                                                      >  2:3750852/1‑149 (MQ=255)

                                                          tatttaCCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTtaattaat                                                                                      >  2:1026242/1‑149 (MQ=255)

                                                           atttaCCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATa                                                                                     <  1:1684455/149‑1 (MQ=255)

                                                            tttaCCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCAGTAACGACAATGAACTAGGGAATTAGTATAAGCATCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATAc                                                                                    >  2:1432595/1‑149 (MQ=255)

                                                              taCCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTa                                                                                  <  2:595686/149‑1 (MQ=255)

                                                               aCCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTAt                                                                                 >  2:575252/1‑149 (MQ=255)

                                                               aCCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTAt                                                                                 >  2:2866592/1‑149 (MQ=255)

                                                                 cTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTAttt                                                                               <  1:2614631/149‑1 (MQ=255)

                                                                  tCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTa                                                                              >  2:2957611/1‑149 (MQ=255)

                                                                  tCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTa                                                                              >  1:3037965/1‑149 (MQ=255)

                                                                  tCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTa                                                                              >  1:3498422/1‑149 (MQ=255)

                                                                   cGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTaa                                                                             >  2:2382309/1‑149 (MQ=255)

                                                                   cGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTaa                                                                             >  1:3336587/1‑149 (MQ=255)

                                                                    gggTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTaaa                                                                            >  2:336689/1‑149 (MQ=255)

                                                                    gggTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTaaa                                                                            >  2:856359/1‑149 (MQ=255)

                                                                    gggTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATATaaa                                                                            >  2:2582625/1‑149 (MQ=255)

                                                                     ggTACTTATTCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAat                                                                           <  2:740988/149‑1 (MQ=255)

                                                                      gTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAata                                                                          <  2:2194611/149‑1 (MQ=255)

                                                                      gTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAata                                                                          <  1:146573/149‑1 (MQ=255)

                                                                      gTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAata                                                                          <  2:2111804/149‑1 (MQ=255)

                                                                       tACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAatat                                                                         >  1:954209/1‑149 (MQ=255)

                                                                       tACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAatat                                                                         >  1:2064494/1‑149 (MQ=255)

                                                                         cTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAAtatta                                                                       >  2:1781678/1‑149 (MQ=255)

                                                                             tGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTAtttt                                                                   >  1:2343627/1‑149 (MQ=255)

                                                                              gCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTg                                                                  >  1:1762696/1‑149 (MQ=255)

                                                                              gCTGATTTTTATTATTAAGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATCAACTAGGGAATTAGTATAATCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTg                                                                  >  2:3497692/1‑149 (MQ=255)

                                                                               cTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGa                                                                 <  1:4005849/149‑1 (MQ=255)

                                                                                 gATTTTTATTATTATGGGGATGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATAATATTTAAATATTATTTTGAGc                                                               <  1:3744901/149‑1 (MQ=255)

                                                                                    ttttATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTTAGCata                                                            >  2:2475180/1‑149 (MQ=255)

                                                                                    ttttATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATAATATTTTGAGCata                                                            <  2:2320119/149‑1 (MQ=255)

                                                                                        attattatGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGca                                                        <  1:823503/149‑1 (MQ=255)

                                                                                        attattatGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGca                                                        <  2:1754034/149‑1 (MQ=255)

                                                                                          tattatGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGcaca                                                      >  1:3738369/1‑149 (MQ=255)

                                                                                          tattatGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGcaca                                                      <  2:3189715/149‑1 (MQ=255)

                                                                                          tattatGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGcaca                                                      >  1:3100998/1‑149 (MQ=255)

                                                                                           attatGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGAACAt                                                     >  1:3223523/1‑149 (MQ=255)

                                                                                            ttatGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATa                                                    <  1:2565199/149‑1 (MQ=255)

                                                                                              atGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAg                                                  <  2:2999697/149‑1 (MQ=255)

                                                                                                  ggAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTg                                              >  1:2669011/1‑149 (MQ=255)

                                                                                                      ggTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTgcgcg                                          >  2:3810688/1‑149 (MQ=255)

                                                                                                       gtgtTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTgcgcgc                                         >  2:2297179/1‑149 (MQ=255)

                                                                                                       gtgtTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTgcgcgc                                         >  1:3491384/1‑149 (MQ=255)

                                                                                                        tgtTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCt                                        <  1:1482972/149‑1 (MQ=255)

                                                                                                         gtTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTa                                       >  2:3512335/1‑149 (MQ=255)

                                                                                                               tatGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGcac                                 >  2:1176361/1‑149 (MQ=255)

                                                                                                               tatGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGcac                                 <  1:3454321/149‑1 (MQ=255)

                                                                                                                atGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGcaca                                >  2:1229760/1‑149 (MQ=255)

                                                                                                                atGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGcaca                                <  1:274519/149‑1 (MQ=255)

                                                                                                                 tGAGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAg                               >  1:2223685/1‑149 (MQ=255)

                                                                                                                   aGTTTCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGAt                             >  2:3231322/1‑149 (MQ=255)

                                                                                                                     tttCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGAttt                           >  1:287859/1‑149 (MQ=255)

                                                                                                                      ttCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTg                          <  2:1550488/149‑1 (MQ=255)

                                                                                                                      ttCATTTATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTg                          <  2:2110083/149‑1 (MQ=255)

                                                                                                                          tttATGCCGTAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCt                      >  1:3257450/1‑149 (MQ=255)

                                                                                                                                   tAACGACAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTttaccttac             >  1:2465356/1‑149 (MQ=255)

                                                                                                                                        aCAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGggc        >  2:445770/1‑149 (MQ=255)

                                                                                                                                        aCAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGggc        >  2:1076897/1‑149 (MQ=255)

                                                                                                                                         cAATGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGggcg       >  2:1581509/1‑149 (MQ=255)

                                                                                                                                          aaTGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGggcgg      >  1:532950/1‑149 (MQ=255)

                                                                                                                                           aTGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGggcggc     >  1:99613/1‑149 (MQ=255)

                                                                                                                                           aTGAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGggcggc     >  1:100326/1‑149 (MQ=255)

                                                                                                                                           aTGAACTAGGGAATAAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATATTGAGCATATGCACATAAGTTTGCGCGCTAAAGCACAGATTTTCGCTTTACCTTAACGggaggc     >  2:902255/1‑149 (MQ=255)

                                                                                                                                             gAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCAc   <  1:858356/149‑1 (MQ=255)

                                                                                                                                             gAACTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCAc   >  1:2496576/1‑149 (MQ=255)

                                                                                                                                              aaCTAGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACt  >  2:3793149/1‑149 (MQ=255)

ATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACT  >  CP009273/3268211‑3268501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`