Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,290,970 A→T 100% R176R (CGT→CGA)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,290,9700AT98.2% 729.2 / 1.9 220R176R (CGT→CGA) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base T (115/101);  total (118/102)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

TAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCAC  >  CP009273/3290826‑3291115
                                                                                                                                                |                                                                                                                                                 
tAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                               <  1:4013873/149‑1 (MQ=255)
tAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                               <  1:1882670/149‑1 (MQ=255)
  gcgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATc                                                                                                                                             <  1:1868196/149‑1 (MQ=255)
  gcgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATc                                                                                                                                             >  1:2697802/1‑149 (MQ=255)
     cccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgcc                                                                                                                                          <  2:3047057/149‑1 (MQ=255)
      ccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgccg                                                                                                                                         >  2:3748300/1‑149 (MQ=255)
       cTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgccgc                                                                                                                                        >  2:3698957/1‑149 (MQ=255)
            ccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAc                                                                                                                                   >  1:3162849/1‑149 (MQ=255)
             cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAcc                                                                                                                                  >  1:1886219/1‑149 (MQ=255)
             cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAcc                                                                                                                                  >  1:3553029/1‑149 (MQ=255)
               gCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAcccc                                                                                                                                <  2:436396/149‑1 (MQ=255)
               gCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAcccc                                                                                                                                >  2:4001878/1‑149 (MQ=255)
               gCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAcccc                                                                                                                                >  1:2364752/1‑149 (MQ=255)
                cGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCa                                                                                                                               >  2:1643213/1‑149 (MQ=255)
                cGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCa                                                                                                                               >  2:1643176/1‑149 (MQ=255)
                cGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCa                                                                                                                               <  1:2884074/149‑1 (MQ=255)
                cGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCa                                                                                                                               <  1:3119189/149‑1 (MQ=255)
                 ggCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAg                                                                                                                              >  2:2800737/1‑149 (MQ=255)
                  gCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGc                                                                                                                             >  2:3486225/1‑149 (MQ=255)
                    tAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCg                                                                                                                           <  1:3865104/149‑1 (MQ=255)
                    tAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCg                                                                                                                           >  2:2526800/1‑149 (MQ=255)
                      aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAg                                                                                                                         <  1:2207/149‑1 (MQ=255)
                      aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAg                                                                                                                         <  1:2148/149‑1 (MQ=255)
                      aaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAg                                                                                                                         >  2:3512033/1‑149 (MQ=255)
                        aaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGa                                                                                                                       >  1:1026887/1‑149 (MQ=255)
                        aaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGa                                                                                                                       <  1:1207134/149‑1 (MQ=255)
                         aaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGAc                                                                                                                      <  1:4001878/149‑1 (MQ=255)
                         aaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTCCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCACAACCCCAGCCGAGGAc                                                                                                                      >  2:1990243/1‑149 (MQ=255)
                          aCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACa                                                                                                                     <  2:1744455/149‑1 (MQ=255)
                          aCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACa                                                                                                                     >  1:483164/1‑149 (MQ=255)
                             cGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAg                                                                                                                  >  2:1494922/1‑149 (MQ=255)
                               cccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGcc                                                                                                                >  2:2924681/1‑149 (MQ=255)
                                 cTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCa                                                                                                              <  2:3077971/149‑1 (MQ=255)
                                     gCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATaaa                                                                                                          <  2:862312/149‑1 (MQ=255)
                                       ttCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAaca                                                                                                        <  2:3263052/149‑1 (MQ=255)
                                           ggTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAg                                                                                                    <  1:472953/149‑1 (MQ=255)
                                           ggTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAg                                                                                                    <  1:1661207/149‑1 (MQ=255)
                                            gTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGc                                                                                                   >  1:3610750/1‑149 (MQ=255)
                                              aTGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGt                                                                                                 <  1:2380570/149‑1 (MQ=255)
                                                gTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCg                                                                                               >  2:3250082/1‑149 (MQ=255)
                                                    aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCgg                                                                                           >  2:3931184/1‑149 (MQ=255)
                                                    aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCACAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCgg                                                                                           >  2:1786510/1‑149 (MQ=255)
                                                     tttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                          >  2:618598/1‑149 (MQ=255)
                                                      ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGt                                                                                         >  2:2465485/1‑149 (MQ=255)
                                                       tCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGtt                                                                                        >  1:406954/1‑149 (MQ=255)
                                                        cGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGttt                                                                                       <  2:763203/149‑1 (MQ=255)
                                                          aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTaa                                                                                     <  1:3350861/149‑1 (MQ=255)
                                                            cGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAaca                                                                                   <  1:3937684/149‑1 (MQ=255)
                                                               tGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCg                                                                                >  1:2352691/1‑149 (MQ=255)
                                                                gCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCgg                                                                               >  2:243398/1‑149 (MQ=255)
                                                                 ccTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGa                                                                              <  2:78739/149‑1 (MQ=255)
                                                                  cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAt                                                                             <  1:1741899/149‑1 (MQ=255)
                                                                  cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAt                                                                             <  1:1656302/149‑1 (MQ=255)
                                                                   tGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAtt                                                                            <  2:1260561/149‑1 (MQ=255)
                                                                       tttGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGccc                                                                        >  1:3725384/1‑149 (MQ=255)
                                                                          gCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATc                                                                     <  1:362816/149‑1 (MQ=255)
                                                                          gCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATc                                                                     <  2:1555383/149‑1 (MQ=255)
                                                                            tgtgtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGACTCGCCGCAAAACCACAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCa                                                                   <  1:2260958/148‑1 (MQ=255)
                                                                               gtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACCAAGCCCATAAACACCCGCGTCGCCGGGGTTAACACCGGATTGCCCATCCAGaa                                                                >  2:2724609/1‑149 (MQ=255)
                                                                                   cACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGcc                                                            <  1:4014006/149‑1 (MQ=255)
                                                                                    aCGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCa                                                           >  1:2301797/1‑149 (MQ=255)
                                                                                     cGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAg                                                          >  1:2481477/1‑149 (MQ=255)
                                                                                       cccccATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgcg                                                        >  2:2246563/3‑149 (MQ=255)
                                                                                        aCCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgcgc                                                       >  1:3834984/1‑149 (MQ=255)
                                                                                          ccATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCAc                                                     >  1:161523/1‑149 (MQ=255)
                                                                                           cATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCAcc                                                    >  2:1016425/1‑149 (MQ=255)
                                                                                              ttttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGcc                                                 <  1:579766/149‑1 (MQ=255)
                                                                                                 tGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCAt                                              <  1:3862346/149‑1 (MQ=255)
                                                                                                  gCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCTGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACATAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCTTCCAGAATGCCAGCGCACCGCCCATc                                             <  2:288578/149‑1 (MQ=255)
                                                                                                    aCCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGa                                           >  1:436417/1‑149 (MQ=255)
                                                                                                    aCCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGa                                           >  1:3574902/1‑149 (MQ=255)
                                                                                                     ccAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGac                                          >  2:3117613/1‑149 (MQ=255)
                                                                                                       aGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCACAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGacac                                        <  2:1661884/149‑1 (MQ=255)
                                                                                                        gCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACAcc                                       <  2:246938/149‑1 (MQ=255)
                                                                                                        gCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACAcc                                       >  1:3682830/1‑149 (MQ=255)
                                                                                                           tCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGt                                    >  1:1498710/1‑149 (MQ=255)
                                                                                                             gCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTg                                  >  1:1791136/1‑149 (MQ=255)
                                                                                                              cAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTgg                                 >  1:1342452/1‑149 (MQ=255)
                                                                                                                aTCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCg                               >  2:1026766/1‑149 (MQ=255)
                                                                                                                  cAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGAc                             >  1:529369/1‑149 (MQ=255)
                                                                                                                      aaCACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCAt                         <  1:622992/149‑1 (MQ=255)
                                                                                                                      aaCACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCAt                         >  2:521622/1‑149 (MQ=255)
                                                                                                                        cacCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                       >  2:660883/1‑149 (MQ=255)
                                                                                                                        cacCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                       >  1:3636853/1‑149 (MQ=255)
                                                                                                                        cacCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                       <  1:3486225/149‑1 (MQ=255)
                                                                                                                         acCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTcc                      <  1:2800737/149‑1 (MQ=255)
                                                                                                                         acCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGACCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGACCATCGACACCTGTTGGCGACGCATTcc                      <  1:3931184/149‑1 (MQ=255)
                                                                                                                          ccATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTccc                     >  2:1876742/1‑149 (MQ=255)
                                                                                                                            aTCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCccgc                   >  1:2551876/1‑149 (MQ=255)
                                                                                                                             tCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCccgcc                  >  2:3905750/1‑149 (MQ=255)
                                                                                                                                  aGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAc             >  1:10663/1‑149 (MQ=255)
                                                                                                                                  aGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAc             <  1:3894525/149‑1 (MQ=255)
                                                                                                                                   gCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAcc            <  2:304620/149‑1 (MQ=255)
                                                                                                                                   gCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAcc            >  2:2157064/1‑149 (MQ=255)
                                                                                                                                   gCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAcc            <  2:2378642/149‑1 (MQ=255)
                                                                                                                                     ccGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCgg          >  1:3510520/1‑149 (MQ=255)
                                                                                                                                     ccGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCgg          >  1:1631828/1‑149 (MQ=255)
                                                                                                                                     ccGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCgg          >  1:1463966/1‑149 (MQ=255)
                                                                                                                                         ccaccaGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGcc      >  1:3124412/1‑149 (MQ=255)
                                                                                                                                           accaGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGc    >  2:2719747/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCAC  >  CP009273/3290826‑3291115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑