Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,306,165 C→A 28.0% V125F (GTT→TTT)  rbfA ← 30s ribosome binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,306,1650CA28.0% 573.3 / 180.9 274V125F (GTT→TTT) rbfA30s ribosome binding factor
Reads supporting (aligned to +/- strand):  ref base C (123/74);  new base A (35/42);  total (158/116)
Fisher's exact test for biased strand distribution p-value = 1.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

ACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGA  >  CP009273/3306017‑3306312
                                                                                                                                                    |                                                                                                                                                   
  ggTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAacac                                                                                                                                                   >  2:112657/1‑149 (MQ=255)
  ggTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAaaac                                                                                                                                                   <  2:2723148/149‑1 (MQ=255)
  ggTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAaaac                                                                                                                                                   >  2:1527333/1‑149 (MQ=255)
  ggTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAaaac                                                                                                                                                   <  1:3484421/149‑1 (MQ=255)
    ttGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTGGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacga                                                                                                                                                 <  2:2385271/149‑1 (MQ=255)
       gCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACAGACCCTGAGGATTATCCAGCAACAAAACGCCGTTAAAGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGTGTTAAAactacga                                                                                                                                              >  2:877788/1‑148 (MQ=255)
         gTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttc                                                                                                                                            >  1:601796/1‑149 (MQ=255)
         gTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttc                                                                                                                                            >  1:35393/1‑149 (MQ=255)
          ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttct                                                                                                                                           >  1:1060170/1‑149 (MQ=255)
          ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttct                                                                                                                                           >  1:3297884/1‑149 (MQ=255)
            atatataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttcttc                                                                                                                                         >  1:2582312/1‑149 (MQ=255)
              atataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGt                                                                                                                                       <  1:2285146/149‑1 (MQ=255)
                ataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCa                                                                                                                                     <  2:1835697/149‑1 (MQ=255)
                 taCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCAt                                                                                                                                    >  1:1966269/1‑149 (MQ=255)
                 taCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGATTATCAAGCAACAAAACGCCGTTACTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTTCTGTCGTCCGGGTTTACACGACTTTCTTCGTCAt                                                                                                                                    <  2:3473560/149‑1 (MQ=255)
                    gTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                                                                                                                 >  1:21523/1‑149 (MQ=255)
                    gTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                                                                                                                 >  2:3381791/1‑149 (MQ=255)
                     tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGttt                                                                                                                                <  1:1744293/149‑1 (MQ=255)
                     tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGttt                                                                                                                                <  1:3684536/149‑1 (MQ=255)
                      ttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTg                                                                                                                               >  2:1086290/1‑149 (MQ=255)
                       tCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGa                                                                                                                              >  2:2205245/1‑149 (MQ=255)
                        cACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGAc                                                                                                                             <  1:2722504/149‑1 (MQ=255)
                        cACTTTTTGCAGCGCATCGTTGCTGGAAATACCCTGAGTTTTATCCAGCAAAAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGAc                                                                                                                             <  1:3142953/149‑1 (MQ=255)
                           tttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCAc                                                                                                                          >  2:1972442/1‑149 (MQ=255)
                             tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGc                                                                                                                        >  2:3556778/1‑149 (MQ=255)
                                gCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                     >  2:554793/1‑149 (MQ=255)
                                 cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGt                                                                                                                    <  1:2509843/149‑1 (MQ=255)
                                    cgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAc                                                                                                                 <  2:1953154/149‑1 (MQ=255)
                                      cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCa                                                                                                               >  1:2407378/1‑149 (MQ=255)
                                      cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCa                                                                                                               <  2:3922920/149‑1 (MQ=255)
                                       aTCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAg                                                                                                              >  1:49509/1‑149 (MQ=255)
                                        tCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAgg                                                                                                             >  1:435975/1‑149 (MQ=255)
                                         cGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGt                                                                                                            <  1:1321026/149‑1 (MQ=255)
                                          gTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGtt                                                                                                           >  1:3717538/1‑149 (MQ=255)
                                          gTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGtg                                                                                                           >  2:3980649/1‑148 (MQ=255)
                                           ttGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                                                                                                          <  1:3016068/149‑1 (MQ=255)
                                           ttGCTGGACATACCCGGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGtgt                                                                                                          >  2:2022122/1‑147 (MQ=255)
                                               tGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACa                                                                                                      <  2:568856/149‑1 (MQ=255)
                                                ggACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                     <  2:2079392/149‑1 (MQ=255)
                                                ggACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                     <  2:494308/149‑1 (MQ=255)
                                                ggACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                     <  2:2284126/149‑1 (MQ=255)
                                                 gACATACCCTGAGGTTTATCCAGCAACAACACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                    <  1:2877522/149‑1 (MQ=255)
                                                 gACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                    <  1:2878093/149‑1 (MQ=255)
                                                  aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgc                                                                                                   <  1:1137597/149‑1 (MQ=255)
                                                    aTACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgcgc                                                                                                 >  2:3611131/1‑149 (MQ=255)
                                                     tACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCa                                                                                                >  1:1953036/1‑149 (MQ=255)
                                                       cccTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATc                                                                                              >  2:1396641/1‑149 (MQ=255)
                                                          tGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCt                                                                                           <  2:964857/149‑1 (MQ=255)
                                                          tGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCt                                                                                           >  1:1844694/1‑149 (MQ=255)
                                                           gAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGACCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCtt                                                                                          >  2:2602733/1‑149 (MQ=255)
                                                            aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTc                                                                                         <  2:4075815/149‑1 (MQ=255)
                                                              gTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCaa                                                                                       >  1:2464293/1‑149 (MQ=255)
                                                                 tATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCa                                                                                    <  2:625877/149‑1 (MQ=255)
                                                                  aTCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCag                                                                                   >  2:3018008/1‑149 (MQ=255)
                                                                  aTCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCag                                                                                   >  1:2839724/1‑149 (MQ=255)
                                                                    ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagag                                                                                 <  2:1213444/149‑1 (MQ=255)
                                                                     cAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagaga                                                                                <  2:1305062/149‑1 (MQ=255)
                                                                       gCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACTCGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCcgagagg                                                                              >  2:2524279/1‑148 (MQ=255)
                                                                       gCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGt                                                                              <  1:1498902/149‑1 (MQ=255)
                                                                          acaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGt                                                                           <  2:3180476/149‑1 (MQ=255)
                                                                           caaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTc                                                                          <  1:2955563/149‑1 (MQ=255)
                                                                            aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCg                                                                         >  1:3830087/1‑149 (MQ=255)
                                                                             aaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        <  2:1614114/149‑1 (MQ=255)
                                                                              aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTa                                                                       >  2:3503479/1‑149 (MQ=255)
                                                                              aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTa                                                                       <  2:2766300/149‑1 (MQ=255)
                                                                                 gCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTagaa                                                                    <  1:3289286/149‑1 (MQ=255)
                                                                                  ccGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTagaag                                                                   >  2:2551098/1‑149 (MQ=255)
                                                                                     ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGaagaag                                                                >  2:2944623/1‑149 (MQ=255)
                                                                                     ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGaagaag                                                                >  1:914402/1‑149 (MQ=255)
                                                                                     ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGaagaag                                                                >  1:1990111/1‑149 (MQ=255)
                                                                                      tAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAgg                                                               <  2:3241672/149‑1 (MQ=255)
                                                                                       aaTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGt                                                              >  1:2734868/1‑149 (MQ=255)
                                                                                        aTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTc                                                             >  2:3774052/1‑149 (MQ=255)
                                                                                             gcgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTc                                                        <  2:2740693/149‑1 (MQ=255)
                                                                                                accgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCgg                                                     >  2:3414781/1‑149 (MQ=255)
                                                                                                   gcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCAc                                                  >  1:1240189/1‑149 (MQ=255)
                                                                                                    cgacgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACg                                                 <  2:3879280/149‑1 (MQ=255)
                                                                                                       cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATa                                              >  2:2905165/1‑149 (MQ=255)
                                                                                                         acgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACg                                            >  2:1887750/1‑149 (MQ=255)
                                                                                                         acgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACg                                            >  2:74606/1‑149 (MQ=255)
                                                                                                            aGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAg                                         >  2:3555059/1‑149 (MQ=255)
                                                                                                             ggACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAgg                                        >  2:2588676/1‑149 (MQ=255)
                                                                                                             ggACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAgg                                        <  1:554793/149‑1 (MQ=255)
                                                                                                               acgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACTACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTATAAGAAGGTCAGTTCCGGCACGATACGCAGgcg                                      >  1:2686200/1‑149 (MQ=255)
                                                                                                               acgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGgcg                                      >  1:1606264/1‑149 (MQ=255)
                                                                                                                 gacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCa                                    >  2:2380621/1‑149 (MQ=255)
                                                                                                                  acTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCAt                                   >  2:172849/1‑149 (MQ=255)
                                                                                                                  acTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCAt                                   <  1:2455775/149‑1 (MQ=255)
                                                                                                                  acTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCAt                                   <  2:1882323/149‑1 (MQ=255)
                                                                                                                        tAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTTGTCACAAGTTTTTACATGCGCATCCCTTCAACCAGAGAGGTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCttt                             >  1:3083464/1‑149 (MQ=255)
                                                                                                                         aGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTc                            <  1:1806293/149‑1 (MQ=255)
                                                                                                                             ctcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCca                        >  1:3893978/1‑149 (MQ=255)
                                                                                                                             ctcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCca                        >  2:301209/1‑149 (MQ=255)
                                                                                                                                ctTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcagca                     <  2:2485240/149‑1 (MQ=255)
                                                                                                                                ctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcagca                     >  2:40044/1‑149 (MQ=255)
                                                                                                                                     tGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTg                >  1:2833530/1‑149 (MQ=255)
                                                                                                                                       tcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCg              >  1:765194/1‑149 (MQ=255)
                                                                                                                                           ccGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATg          <  2:435975/149‑1 (MQ=255)
                                                                                                                                                ttAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAcc     >  1:1005870/1‑149 (MQ=255)
                                                                                                                                                ttAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAcc     >  2:2251959/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGA  >  CP009273/3306017‑3306312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑