Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,317,805 C→A 30.8% D156Y (GAT→TAT)  folP ← 7,8‑dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,317,8050CA30.8% 460.9 / 183.7 246D156Y (GAT→TAT) folP7,8‑dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (83/87);  new base A (40/36);  total (123/123)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

GCCAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCT  >  CP009273/3317657‑3317953
                                                                                                                                                    |                                                                                                                                                    
 ccAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCg                                                                                                                                                     <  2:792289/149‑1 (MQ=255)
 ccAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCg                                                                                                                                                     <  2:2853812/149‑1 (MQ=255)
      cgcgcCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATa                                                                                                                                                <  1:3103896/149‑1 (MQ=255)
      cgcgcCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATa                                                                                                                                                <  1:3072290/149‑1 (MQ=255)
          ccAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTc                                                                                                                                            <  2:512324/149‑1 (MQ=255)
          ccAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTc                                                                                                                                            <  2:1780389/149‑1 (MQ=255)
             gTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCTGa                                                                                                                                         >  2:2681215/1‑149 (MQ=255)
               aaTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGc                                                                                                                                       >  2:3461218/1‑149 (MQ=255)
                aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCt                                                                                                                                      <  1:2611628/149‑1 (MQ=255)
                aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCt                                                                                                                                      >  2:523385/1‑149 (MQ=255)
                aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCt                                                                                                                                      <  1:2042918/149‑1 (MQ=255)
                 tGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCtt                                                                                                                                     >  1:2879890/1‑149 (MQ=255)
                  gAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTc                                                                                                                                    <  2:1655141/149‑1 (MQ=255)
                  gAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTc                                                                                                                                    >  2:1864367/1‑149 (MQ=255)
                   aaTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTcc                                                                                                                                   >  2:3937512/1‑149 (MQ=255)
                   aaTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTcc                                                                                                                                   <  1:1539566/149‑1 (MQ=255)
                   aaTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTcc                                                                                                                                   <  1:3585521/149‑1 (MQ=255)
                   aaTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTcc                                                                                                                                   <  2:1239628/149‑1 (MQ=255)
                       gTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCa                                                                                                                               >  1:1053128/1‑149 (MQ=255)
                       gTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCa                                                                                                                               <  2:167891/149‑1 (MQ=255)
                       gTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCa                                                                                                                               <  1:320736/149‑1 (MQ=255)
                        ttATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCAt                                                                                                                              >  2:1842787/1‑149 (MQ=255)
                            gggAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGtt                                                                                                                          >  2:2633852/1‑149 (MQ=255)
                            gggAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTGTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGtt                                                                                                                          <  2:2960531/149‑1 (MQ=255)
                               agagaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGttttt                                                                                                                       <  1:4003697/149‑1 (MQ=255)
                                gagaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTg                                                                                                                      <  1:1682844/149‑1 (MQ=255)
                                  gaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGa                                                                                                                    <  1:411636/149‑1 (MQ=255)
                                   aTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGAt                                                                                                                   <  2:4059160/149‑1 (MQ=255)
                                         accgaaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCt                                                                                                             >  1:865001/1‑149 (MQ=255)
                                            gaaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCa                                                                                                          >  1:1943436/1‑149 (MQ=255)
                                              aaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCata                                                                                                        >  2:3066959/1‑149 (MQ=255)
                                              aaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCata                                                                                                        >  2:1010013/1‑149 (MQ=255)
                                               accgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCatat                                                                                                       >  2:3658913/1‑149 (MQ=255)
                                                 cgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGc                                                                                                     <  2:315314/149‑1 (MQ=255)
                                                  gaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCa                                                                                                    <  2:229043/149‑1 (MQ=255)
                                                   aaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCATCGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACCTCGTCATAATTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCAt                                                                                                   <  1:3741877/149‑1 (MQ=255)
                                                   aaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCAt                                                                                                   >  2:3602120/1‑149 (MQ=255)
                                                    aTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATc                                                                                                  <  1:3937506/149‑1 (MQ=255)
                                                     tCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCa                                                                                                 <  2:437670/149‑1 (MQ=255)
                                                     tCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCa                                                                                                 <  2:2485501/149‑1 (MQ=255)
                                                     tCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCa                                                                                                 <  2:2097712/149‑1 (MQ=255)
                                                       cGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGa                                                                                               >  2:3893298/1‑149 (MQ=255)
                                                        ggggTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGAc                                                                                              >  2:881083/1‑149 (MQ=255)
                                                                gCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggt                                                                                      >  1:1852715/1‑149 (MQ=255)
                                                                gCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggt                                                                                      >  1:3500893/1‑149 (MQ=255)
                                                                   acaaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtaaa                                                                                   >  1:2242423/1‑149 (MQ=255)
                                                                     aaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtacacc                                                                                 >  2:2403603/1‑149 (MQ=255)
                                                                       tttCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtaaaccgg                                                                               <  1:565321/149‑1 (MQ=255)
                                                                         tctcTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGtt                                                                             <  1:1503888/149‑1 (MQ=255)
                                                                            ctTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCt                                                                          <  1:3326878/149‑1 (MQ=255)
                                                                               ttGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTACACCGGTTTCTGCa                                                                       >  2:3171183/1‑149 (MQ=255)
                                                                               ttGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTACACCGGTTTCTGCa                                                                       >  2:1401660/1‑149 (MQ=255)
                                                                                 gCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAgc                                                                     >  2:3716820/1‑149 (MQ=255)
                                                                                   gATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAgccg                                                                   >  2:686096/1‑149 (MQ=255)
                                                                                   gATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAgccg                                                                   >  2:685982/1‑149 (MQ=255)
                                                                                      aCCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCt                                                                >  2:1100319/1‑149 (MQ=255)
                                                                                       cccGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTc                                                               <  1:2393589/149‑1 (MQ=255)
                                                                                       cccGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTc                                                               <  2:400568/149‑1 (MQ=255)
                                                                                       cccGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTc                                                               <  2:1066889/149‑1 (MQ=255)
                                                                                         cGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCa                                                             <  2:1386021/149‑1 (MQ=255)
                                                                                         cGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCa                                                             >  1:3431266/1‑149 (MQ=255)
                                                                                         cGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCa                                                             <  2:2195351/149‑1 (MQ=255)
                                                                                             tGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAgc                                                         >  1:2619731/1‑149 (MQ=255)
                                                                                                  gCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAg                                                    <  1:2069513/149‑1 (MQ=255)
                                                                                                  gCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAg                                                    >  1:2178944/1‑149 (MQ=255)
                                                                                                     aCGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGtt                                                 >  2:885394/1‑149 (MQ=255)
                                                                                                     aCGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGtt                                                 <  2:60672/149‑1 (MQ=255)
                                                                                                          cTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGaa                                            <  2:1363330/149‑1 (MQ=255)
                                                                                                          cTATTTGCTAAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGaa                                            <  1:1738044/149‑1 (MQ=255)
                                                                                                              ttGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAggg                                        >  2:768570/1‑149 (MQ=255)
                                                                                                               tGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGa                                       >  1:2806817/1‑149 (MQ=255)
                                                                                                                 cTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGc                                     <  2:2105455/149‑1 (MQ=255)
                                                                                                                 cTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGc                                     >  2:3266852/1‑149 (MQ=255)
                                                                                                                  tCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCg                                    >  2:1600483/1‑149 (MQ=255)
                                                                                                                    aaTAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGa                                  <  2:1738659/149‑1 (MQ=255)
                                                                                                                    aaTAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGa                                  <  1:643335/149‑1 (MQ=255)
                                                                                                                     aTAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGat                                 <  1:3817893/149‑1 (MQ=255)
                                                                                                                     aTAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGat                                 <  1:3816823/149‑1 (MQ=255)
                                                                                                                     aTAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGat                                 <  2:1117610/149‑1 (MQ=255)
                                                                                                                      tAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGata                                >  1:250170/1‑149 (MQ=255)
                                                                                                                       aaaGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGatat                               >  1:83812/1‑149 (MQ=255)
                                                                                                                        aaGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGACAGGGCGCGGATATc                              >  2:3872728/1‑149 (MQ=255)
                                                                                                                          gTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTCAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                            >  1:2815594/1‑149 (MQ=255)
                                                                                                                          gTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                            <  1:778610/149‑1 (MQ=255)
                                                                                                                            aGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTCTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATta                          >  1:799196/1‑149 (MQ=255)
                                                                                                                                 ttCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTaataat                     <  1:1479569/149‑1 (MQ=255)
                                                                                                                                 ttCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTaataat                     >  2:3555565/1‑149 (MQ=255)
                                                                                                                                  tCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAAtg                    >  2:805999/1‑149 (MQ=255)
                                                                                                                                  tCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAAtg                    <  1:2913856/149‑1 (MQ=255)
                                                                                                                                     cTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGa                 <  2:1344167/149‑1 (MQ=255)
                                                                                                                                      ttCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAg                <  1:2277540/149‑1 (MQ=255)
                                                                                                                                       tCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAgc               <  1:685982/149‑1 (MQ=255)
                                                                                                                                             aaGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAAc         >  2:1289260/1‑149 (MQ=255)
                                                                                                                                              aGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACt        >  1:1239538/1‑149 (MQ=255)
                                                                                                                                              aGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACt        <  1:100343/149‑1 (MQ=255)
                                                                                                                                               gACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACtt       >  2:593974/1‑149 (MQ=255)
                                                                                                                                                  aTCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCg    >  2:2920647/1‑149 (MQ=255)
                                                                                                                                                  aTCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCg    >  2:860239/1‑149 (MQ=255)
                                                                                                                                                   tCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGc   >  2:1499808/1‑149 (MQ=255)
                                                                                                                                                    cGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCt  <  1:1154191/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCT  >  CP009273/3317657‑3317953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑