Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,334,687 G→T 100% A21A (GCG→GCT)  kdsD → D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,334,6870GT99.6% 781.3 / ‑4.4 232A21A (GCG→GCT) kdsDD‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (118/113);  total (119/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

TATTATTAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCA  >  CP009273/3334541‑3334831
                                                                                                                                                  |                                                                                                                                                
tattatTAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTAt                                                                                                                                                <  1:4065465/149‑1 (MQ=255)
 attattAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTAtt                                                                                                                                               <  1:2709135/149‑1 (MQ=255)
  ttattaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTg                                                                                                                                              >  1:2471583/1‑149 (MQ=255)
   tattaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGa                                                                                                                                             >  1:2247649/1‑149 (MQ=255)
   tattaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGa                                                                                                                                             >  2:1274053/1‑149 (MQ=255)
    attaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGaa                                                                                                                                            <  2:1895299/149‑1 (MQ=255)
     ttaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAAc                                                                                                                                           >  1:3188853/1‑149 (MQ=255)
       aaCTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGt                                                                                                                                         <  2:721998/149‑1 (MQ=255)
          tGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGaa                                                                                                                                      >  2:13321/1‑149 (MQ=255)
           ggCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAAt                                                                                                                                     >  2:1597160/1‑149 (MQ=255)
            gCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATg                                                                                                                                    >  1:1553345/1‑149 (MQ=255)
              ggATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGcc                                                                                                                                  >  2:788650/1‑149 (MQ=255)
                aTTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTg                                                                                                                                <  1:3745145/149‑1 (MQ=255)
                 tttATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTgg                                                                                                                               <  1:1802262/149‑1 (MQ=255)
                 tttATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTgg                                                                                                                               >  2:2019446/1‑149 (MQ=255)
                    aTCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCgg                                                                                                                            <  1:1000867/149‑1 (MQ=255)
                    aTCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCgg                                                                                                                            >  1:3111450/1‑149 (MQ=255)
                     tCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGa                                                                                                                           >  1:1017022/1‑149 (MQ=255)
                       gTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGc                                                                                                                         <  1:1494705/149‑1 (MQ=255)
                        tATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCt                                                                                                                        >  1:3936428/1‑149 (MQ=255)
                        tATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCt                                                                                                                        <  1:2161171/149‑1 (MQ=255)
                         aTGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCtt                                                                                                                       >  1:222676/1‑149 (MQ=255)
                            gctggcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGAt                                                                                                                    <  1:3382388/149‑1 (MQ=255)
                            gctggcGATGTTGTACTGGTTATCGACAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCTGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGAt                                                                                                                    <  2:1117635/149‑1 (MQ=255)
                               ggcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCaa                                                                                                                 <  1:1973907/149‑1 (MQ=255)
                               ggcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCaa                                                                                                                 <  1:789971/149‑1 (MQ=255)
                                gcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAAt                                                                                                                <  1:2917391/149‑1 (MQ=255)
                                   aTGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACa                                                                                                             <  1:2057395/149‑1 (MQ=255)
                                     gttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACatc                                                                                                           <  1:2035232/149‑1 (MQ=255)
                                     gttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACatc                                                                                                           >  2:1832947/1‑149 (MQ=255)
                                            tGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGa                                                                                                    >  2:1617694/1‑149 (MQ=255)
                                               ttATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAAtt                                                                                                 <  1:806705/149‑1 (MQ=255)
                                                 aTCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTc                                                                                               >  2:2159247/1‑149 (MQ=255)
                                                 aTCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTc                                                                                               >  2:2230486/1‑149 (MQ=255)
                                                  tCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCa                                                                                              >  2:2320958/1‑149 (MQ=255)
                                                  tCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCa                                                                                              <  1:3628369/149‑1 (MQ=255)
                                                   cGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCAc                                                                                             <  1:3469106/149‑1 (MQ=255)
                                                    gCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCTTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACg                                                                                            >  1:2682785/1‑149 (MQ=255)
                                                    gCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACg                                                                                            >  2:303276/1‑149 (MQ=255)
                                                     ccAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACga                                                                                           <  1:2715050/149‑2 (MQ=255)
                                                     ccAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACga                                                                                           <  1:2715379/149‑2 (MQ=255)
                                                      cAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCt                                                                                          <  1:187599/149‑1 (MQ=255)
                                                      cAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAAGCAGAATTTCACGCt                                                                                          >  2:2608655/1‑149 (MQ=255)
                                                         tACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGc                                                                                       >  2:2446316/1‑149 (MQ=255)
                                                          aCTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGcc                                                                                      <  1:3987624/149‑1 (MQ=255)
                                                           cTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCt                                                                                     <  1:571239/149‑1 (MQ=255)
                                                            tCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtg                                                                                    >  2:797911/1‑149 (MQ=255)
                                                            tCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtg                                                                                    <  2:3776998/149‑1 (MQ=255)
                                                             cGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtgt                                                                                   >  1:2129787/1‑149 (MQ=255)
                                                              gTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtgtg                                                                                  >  1:2491023/1‑149 (MQ=255)
                                                              gTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtgtg                                                                                  >  2:459283/1‑149 (MQ=255)
                                                                 gAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaa                                                                               <  2:2574150/149‑1 (MQ=255)
                                                                   aTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAg                                                                             >  2:651119/1‑149 (MQ=255)
                                                                    tAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGa                                                                            >  1:2821954/1‑149 (MQ=255)
                                                                       cTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGt                                                                         <  2:4035957/149‑1 (MQ=255)
                                                                         ggAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTc                                                                       >  1:2272416/1‑149 (MQ=255)
                                                                          gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCt                                                                      <  1:71083/149‑1 (MQ=255)
                                                                            aaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTgg                                                                    >  2:3358495/1‑149 (MQ=255)
                                                                            aaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTgg                                                                    >  2:2422634/1‑149 (MQ=255)
                                                                            aaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTgg                                                                    <  1:2419407/149‑1 (MQ=255)
                                                                            aaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTgg                                                                    <  1:359904/149‑1 (MQ=255)
                                                                              cGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGgtg                                                                  >  2:3899711/1‑149 (MQ=255)
                                                                                  ttATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                              >  1:3650913/1‑149 (MQ=255)
                                                                                   tATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAg                                                             <  2:394991/149‑1 (MQ=255)
                                                                                    aTGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAATCATGTAAATAAGTGCTGGCTATTGATAGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGTTGTAAAgg                                                            <  1:2708611/149‑1 (MQ=255)
                                                                                    aTGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGATCTTGATCAATACATCAATCAGAATTTCACTCTTGCCTGTGAAAAGATGTTCTGGTGTAAAgg                                                            <  1:648775/149‑1 (MQ=255)
                                                                                    aTGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAgg                                                            <  1:3045305/149‑1 (MQ=255)
                                                                                    aTGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAgg                                                            >  2:2242199/1‑149 (MQ=255)
                                                                                        cGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGaaa                                                        <  1:640518/149‑1 (MQ=255)
                                                                                             gTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgt                                                   <  1:13321/149‑1 (MQ=255)
                                                                                                gagTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgt                                                >  2:4097664/1‑149 (MQ=255)
                                                                                                gagTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgt                                                >  2:3119385/1‑149 (MQ=255)
                                                                                                  gTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGACAGTTGTCGTCa                                              >  2:933480/1‑149 (MQ=255)
                                                                                                   ttACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCAt                                             >  2:1585621/1‑149 (MQ=255)
                                                                                                    tACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATg                                            >  2:2984868/1‑149 (MQ=255)
                                                                                                      cAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATggg                                          <  2:2876980/149‑1 (MQ=255)
                                                                                                        aCCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGa                                        >  2:255855/1‑149 (MQ=255)
                                                                                                             gTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGa                                   >  1:887907/1‑149 (MQ=255)
                                                                                                              ttttGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGaa                                  >  1:1197147/1‑149 (MQ=255)
                                                                                                                 tGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAAt                               <  2:826978/149‑1 (MQ=255)
                                                                                                                  gACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATc                              <  2:2752831/149‑1 (MQ=255)
                                                                                                                  gACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATc                              >  1:2428186/1‑149 (MQ=255)
                                                                                                                   aCTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCg                             <  1:2320958/149‑1 (MQ=255)
                                                                                                                     tttCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCggg                           <  2:410196/149‑1 (MQ=255)
                                                                                                                       tCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGc                         >  2:1369022/1‑149 (MQ=255)
                                                                                                                        cAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCa                        >  1:3860350/1‑149 (MQ=255)
                                                                                                                           caagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCatat                     <  1:1604118/149‑1 (MQ=255)
                                                                                                                           caagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCatat                     <  1:2358820/149‑1 (MQ=255)
                                                                                                                            aagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATAtt                    >  2:3441302/1‑149 (MQ=255)
                                                                                                                            aagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATAtt                    >  2:506523/1‑149 (MQ=255)
                                                                                                                                aGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGc                <  2:1144710/149‑1 (MQ=255)
                                                                                                                                aGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGc                >  1:3874189/1‑149 (MQ=255)
                                                                                                                                  gTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGa              >  2:2731689/1‑149 (MQ=255)
                                                                                                                                   tAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaa             <  1:3195638/149‑1 (MQ=255)
                                                                                                                                    aaaGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaa            >  1:3545921/1‑149 (MQ=255)
                                                                                                                                       gaagTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTTTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAAt         >  2:3050750/1‑149 (MQ=255)
                                                                                                                                       gaagTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAAt         >  1:731272/1‑149 (MQ=255)
                                                                                                                                       gaagTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAAt         >  2:3215031/1‑149 (MQ=255)
                                                                                                                                         agTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATgg       >  2:63685/1‑149 (MQ=255)
                                                                                                                                         agTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATgg       <  1:3280820/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TATTATTAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCA  >  CP009273/3334541‑3334831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑