Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,351,650 G→T 100% E1189D (GAG→GAT)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,351,6500GT98.6% 719.4 / ‑0.8 223E1189D (GAG→GAT) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (77/142);  total (78/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

GAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTC  >  CP009273/3351504‑3351795
                                                                                                                                                  |                                                                                                                                                 
gAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATAc                                                                                                                                                 >  1:1819604/1‑149 (MQ=255)
 aGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACt                                                                                                                                                >  2:3967410/1‑149 (MQ=255)
  gTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTg                                                                                                                                               >  2:3313197/1‑149 (MQ=255)
       tCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGaa                                                                                                                                          <  2:3909686/149‑1 (MQ=255)
        cGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAAc                                                                                                                                         <  2:2673145/149‑1 (MQ=255)
          cccGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCg                                                                                                                                       <  2:2366168/149‑1 (MQ=255)
           ccGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGc                                                                                                                                      >  2:768647/1‑149 (MQ=255)
             gTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCAt                                                                                                                                    >  1:1151872/1‑149 (MQ=255)
              tGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                   <  2:2410246/149‑1 (MQ=255)
              tGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                   >  1:3825103/1‑149 (MQ=255)
              tGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                   <  2:2765201/149‑1 (MQ=255)
               gAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATcc                                                                                                                                  <  2:4054982/149‑1 (MQ=255)
               gAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATcc                                                                                                                                  <  2:627626/149‑1 (MQ=255)
                 aaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAg                                                                                                                                <  2:399724/149‑1 (MQ=255)
                       cgAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAgg                                                                                                                          >  2:517703/1‑149 (MQ=255)
                        gAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGc                                                                                                                         >  2:2267962/1‑149 (MQ=255)
                        gAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGc                                                                                                                         <  1:1620686/149‑1 (MQ=255)
                            tGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCActc                                                                                                                     >  1:2491676/1‑149 (MQ=255)
                               tGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTAc                                                                                                                  <  1:1823473/149‑1 (MQ=255)
                                ggCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACt                                                                                                                 <  1:1260011/149‑1 (MQ=255)
                                  cacaGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGc                                                                                                               <  2:460575/149‑1 (MQ=255)
                                  cacaGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGc                                                                                                               <  2:1520844/149‑1 (MQ=255)
                                  cacaGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGc                                                                                                               >  1:2825350/1‑149 (MQ=255)
                                  cacaGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGc                                                                                                               <  2:2528190/149‑1 (MQ=255)
                                             gTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaa                                                                                                    >  2:674017/1‑149 (MQ=255)
                                              tAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaac                                                                                                   <  2:986947/149‑1 (MQ=255)
                                               aaCACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAAcc                                                                                                  <  1:2161466/149‑1 (MQ=255)
                                               aaCACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAAcc                                                                                                  <  2:1240269/149‑1 (MQ=255)
                                                acacGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAAccc                                                                                                 <  1:1711542/149‑1 (MQ=255)
                                                     tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                                                                                            <  1:3516009/149‑1 (MQ=255)
                                                      cTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGtt                                                                                           <  2:897338/149‑1 (MQ=255)
                                                       tggtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGttt                                                                                          <  2:3413104/149‑1 (MQ=255)
                                                        ggtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTg                                                                                         <  1:1065591/149‑1 (MQ=255)
                                                         gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAACCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGa                                                                                        <  2:2875183/149‑1 (MQ=255)
                                                         gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGa                                                                                        <  1:195483/149‑1 (MQ=255)
                                                         gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGa                                                                                        <  1:3391440/149‑1 (MQ=255)
                                                          tggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt                                                                                       <  2:1046222/149‑1 (MQ=255)
                                                           ggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATa                                                                                      >  1:2079321/1‑149 (MQ=255)
                                                           ggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATa                                                                                      >  1:2989304/1‑149 (MQ=255)
                                                            gATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATaa                                                                                     >  2:1988118/1‑149 (MQ=255)
                                                            gATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATaa                                                                                     <  1:887941/149‑1 (MQ=255)
                                                             aTCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                    >  1:3943198/1‑149 (MQ=255)
                                                                tGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGc                                                                                 >  2:1105613/1‑149 (MQ=255)
                                                                 gATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                                >  2:3975681/1‑149 (MQ=255)
                                                                 gATGGGTCGCACCGAACTGCTGAAAGTGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTTTCGAAGCTGCTGGATACTGCCGAACCGAATCCAGGTAAGGCACTCTACTTCACCGAAAACAACCAGCCGTTTGATAACGGcc                                                                                <  2:2432338/149‑1 (MQ=255)
                                                                  aTTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCct                                                                               >  1:3340165/1‑149 (MQ=255)
                                                                     ggTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCctgct                                                                            <  1:1985097/149‑1 (MQ=255)
                                                                        cGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGaa                                                                         <  2:3571946/149‑1 (MQ=255)
                                                                        cGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGaa                                                                         <  2:11560/149‑1 (MQ=255)
                                                                        cGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGaa                                                                         <  2:2855199/149‑1 (MQ=255)
                                                                          cACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcg                                                                       <  2:1620501/149‑1 (MQ=255)
                                                                          cACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcg                                                                       >  2:3689308/1‑149 (MQ=255)
                                                                            ccGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg                                                                     <  2:612178/149‑1 (MQ=255)
                                                                             cGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACgcgc                                                                    >  1:2929500/1‑149 (MQ=255)
                                                                              gACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCa                                                                   >  1:2873128/1‑149 (MQ=255)
                                                                                ccTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGt                                                                 <  1:755678/149‑1 (MQ=255)
                                                                                  tgctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtg                                                               >  2:957878/1‑149 (MQ=255)
                                                                                   gctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgc                                                              >  1:451874/1‑149 (MQ=255)
                                                                                   gctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgc                                                              >  1:451852/1‑149 (MQ=255)
                                                                                    ctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgct                                                             <  2:4061242/149‑1 (MQ=255)
                                                                                    ctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTACCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgct                                                             <  1:2732019/149‑1 (MQ=255)
                                                                                         agagCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAAc                                                        <  1:3348638/149‑1 (MQ=255)
                                                                                           agCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAg                                                      >  2:2582031/1‑149 (MQ=255)
                                                                                           agCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAg                                                      <  1:1130374/149‑1 (MQ=255)
                                                                                                gACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaa                                                 >  1:1508693/1‑149 (MQ=255)
                                                                                                 aCGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaaa                                                <  2:1949105/149‑1 (MQ=255)
                                                                                                 aCGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaaa                                                <  2:303798/149‑1 (MQ=255)
                                                                                                     tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt                                            >  1:1516360/1‑149 (MQ=255)
                                                                                                     tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt                                            >  1:2568511/1‑149 (MQ=255)
                                                                                                      ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGtt                                           >  2:2497883/1‑149 (MQ=255)
                                                                                                      ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGtt                                           >  2:3221620/1‑149 (MQ=255)
                                                                                                        cACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTg                                         <  2:2259752/149‑1 (MQ=255)
                                                                                                         aCCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGt                                        <  2:1188558/149‑1 (MQ=255)
                                                                                                            gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGa                                     <  1:691969/149‑1 (MQ=255)
                                                                                                            gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGa                                     <  1:691453/149‑1 (MQ=255)
                                                                                                            gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGa                                     <  1:2191834/149‑1 (MQ=255)
                                                                                                              cAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATg                                   >  1:3717528/1‑149 (MQ=255)
                                                                                                              cAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATg                                   <  1:1757014/149‑1 (MQ=255)
                                                                                                                      agAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCag                           >  2:2923535/1‑149 (MQ=255)
                                                                                                                         aGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAGAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGc                        <  2:2627844/149‑1 (MQ=255)
                                                                                                                         aGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCCGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGc                        >  1:1465843/1‑149 (MQ=255)
                                                                                                                              gcgcTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAc                   <  1:1818681/149‑1 (MQ=255)
                                                                                                                              gcgcTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAc                   >  2:536878/1‑149 (MQ=255)
                                                                                                                                gcTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCt                 <  2:3829763/149‑1 (MQ=255)
                                                                                                                                 cTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCtt                <  2:1803480/149‑1 (MQ=255)
                                                                                                                                   gTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCt              <  1:2301567/149‑1 (MQ=255)
                                                                                                                                   gTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCt              <  2:2051616/149‑1 (MQ=255)
                                                                                                                                    tCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTg             <  2:2980713/149‑1 (MQ=255)
                                                                                                                                     cGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTgg            <  2:1131926/149‑1 (MQ=255)
                                                                                                                                      gAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGt           >  1:1862816/1‑149 (MQ=255)
                                                                                                                                       aaGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt          >  1:3733620/1‑149 (MQ=255)
                                                                                                                                       aaGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt          >  1:3989183/1‑149 (MQ=255)
                                                                                                                                       aaGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt          <  1:608102/149‑1 (MQ=255)
                                                                                                                                        aGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc         >  2:839131/1‑149 (MQ=255)
                                                                                                                                          ctgctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGa       <  2:317398/149‑1 (MQ=255)
                                                                                                                                          ctgctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGa       <  2:1409661/149‑1 (MQ=255)
                                                                                                                                           tgctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGat      <  2:378/149‑1 (MQ=255)
                                                                                                                                              tgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATAtt   <  2:1285029/149‑1 (MQ=255)
                                                                                                                                              tgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATAtt   <  2:2163503/149‑1 (MQ=255)
                                                                                                                                               ggATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTc  >  1:3984194/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTC  >  CP009273/3351504‑3351795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑