Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,407,969 C→A 100% N249K (AAC→AAA)  acrE → cytoplasmic membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,407,9690CA95.5% 699.9 / 21.1 243N249K (AAC→AAA) acrEcytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (6/5);  new base A (119/113);  total (125/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 245 total aligned reads displayed.

GAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAA  >  CP009273/3407823‑3408116
                                                                                                                                                  |                                                                                                                                                   
  aCTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTc                                                                                                                                                 >  1:1631488/1‑149 (MQ=255)
   cTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCa                                                                                                                                                >  1:1913003/1‑149 (MQ=255)
     ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaaa                                                                                                                                              <  1:1464823/149‑1 (MQ=255)
        gACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCGGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCt                                                                                                                                           <  2:3216767/149‑1 (MQ=255)
        gACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCt                                                                                                                                           <  2:947672/149‑1 (MQ=255)
        gACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCt                                                                                                                                           <  2:649749/149‑1 (MQ=255)
        gACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCt                                                                                                                                           <  1:2317230/149‑1 (MQ=255)
         aCTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTa                                                                                                                                          >  2:1904219/1‑149 (MQ=255)
          cTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTAt                                                                                                                                         <  2:1822653/149‑1 (MQ=255)
           tGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAACTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCtctc                                                                                                                                        >  1:2015177/1‑149 (MQ=255)
           tGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATc                                                                                                                                        <  2:486970/149‑1 (MQ=255)
              ccAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcccc                                                                                                                                     <  2:3874851/149‑1 (MQ=255)
              ccAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcccc                                                                                                                                     <  2:1239514/149‑1 (MQ=255)
               cagcagCTCGATCCTAGCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTTGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCt                                                                                                                                    <  2:1566653/149‑1 (MQ=255)
                  cagcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGaa                                                                                                                                 >  2:3408441/1‑149 (MQ=255)
                   agcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGaaa                                                                                                                                <  2:3758934/149‑1 (MQ=255)
                    gcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAg                                                                                                                               >  1:1861828/1‑149 (MQ=255)
                     cTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAgg                                                                                                                              <  1:3768705/149‑1 (MQ=255)
                         aTCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACg                                                                                                                          >  1:2570307/1‑149 (MQ=255)
                          tCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGc                                                                                                                         <  2:1791551/149‑1 (MQ=255)
                          tCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGc                                                                                                                         >  1:342146/1‑149 (MQ=255)
                          tCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGc                                                                                                                         >  2:3947023/1‑149 (MQ=255)
                          tCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACATATCCCCTGAAAGGTACGc                                                                                                                         >  2:1910739/1‑149 (MQ=255)
                                   cGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct                                                                                                                <  2:539770/149‑1 (MQ=255)
                                    gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtctc                                                                                                               >  2:1454679/1‑149 (MQ=255)
                                       gATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGa                                                                                                            >  2:3236901/1‑149 (MQ=255)
                                        aTGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAt                                                                                                           >  1:3354149/1‑149 (MQ=255)
                                          gtgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgt                                                                                                         <  1:3855007/149‑1 (MQ=255)
                                           tgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgtg                                                                                                        >  1:3882121/1‑149 (MQ=255)
                                           tgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAAAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgtg                                                                                                        <  1:2039049/149‑1 (MQ=255)
                                            gACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGa                                                                                                       <  2:2464005/149‑1 (MQ=255)
                                            gACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGa                                                                                                       <  1:2929530/149‑1 (MQ=255)
                                               ccAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCg                                                                                                    <  2:3521704/149‑1 (MQ=255)
                                                 aaTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGtt                                                                                                  >  2:1042616/1‑149 (MQ=255)
                                                   tCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtga                                                                                                <  2:2161272/149‑1 (MQ=255)
                                                   tCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtga                                                                                                <  2:2842873/149‑1 (MQ=255)
                                                     cAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtgatg                                                                                              <  2:1700866/149‑1 (MQ=255)
                                                       ccAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGaa                                                                                            <  1:2834035/148‑1 (MQ=255)
                                                         aaCGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAg                                                                                          >  1:3395725/1‑149 (MQ=255)
                                                            gATTTTTTGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAc                                                                                       <  1:1529169/149‑1 (MQ=255)
                                                            gATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAACACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAc                                                                                       <  1:2463550/149‑1 (MQ=255)
                                                                 tATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCt                                                                                  <  2:1471896/149‑1 (MQ=255)
                                                                    gAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCa                                                                               <  2:499910/149‑1 (MQ=255)
                                                                     aGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCAt                                                                              >  2:1123583/1‑149 (MQ=255)
                                                                           aaGAAATCCGTAGAGCAAGTAAATTTGAATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCt                                                                        <  1:1889591/149‑1 (MQ=255)
                                                                            aGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTa                                                                       <  1:2977380/149‑1 (MQ=255)
                                                                                 tCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGc                                                                  >  2:1830652/1‑149 (MQ=255)
                                                                                 tCCGTAGAGCAAGGAAATTTGAATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGc                                                                  <  1:1952840/149‑1 (MQ=255)
                                                                                  ccGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCt                                                                 <  1:2683440/149‑1 (MQ=255)
                                                                                    gTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt                                                               <  2:221215/149‑1 (MQ=255)
                                                                                     tAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTc                                                              <  2:2347599/149‑1 (MQ=255)
                                                                                         gCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTcc                                                          >  1:166624/1‑149 (MQ=255)
                                                                                          cAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTccc                                                         <  2:591670/149‑1 (MQ=255)
                                                                                           aaGGACATTTGCATAAGGAAAACGCCTCCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACATATCCCCTTAAAGGTACGCTGCATTTCTCCGATGTGACCGTTGATGAATGGACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                        <  2:334554/149‑1 (MQ=255)
                                                                                           aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                        <  2:2604978/149‑1 (MQ=255)
                                                                                           aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                        <  2:396335/149‑1 (MQ=255)
                                                                                           aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                        >  1:1839308/1‑149 (MQ=255)
                                                                                           aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                        >  2:2067945/1‑149 (MQ=255)
                                                                                             ggAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTaa                                                      <  2:2277465/149‑1 (MQ=255)
                                                                                             ggAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTaa                                                      >  1:47713/1‑149 (MQ=255)
                                                                                                aaTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAAccc                                                   <  1:1299202/149‑1 (MQ=255)
                                                                                                aaTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAAccc                                                   >  1:3516078/1‑149 (MQ=255)
                                                                                                 aTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCg                                                  >  1:2098494/1‑149 (MQ=255)
                                                                                                 aTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCg                                                  >  1:2267251/1‑149 (MQ=255)
                                                                                                      cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACa                                             <  1:3372348/149‑1 (MQ=255)
                                                                                                      cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACa                                             <  1:3672497/149‑1 (MQ=255)
                                                                                                      cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACa                                             <  2:951446/149‑1 (MQ=255)
                                                                                                      cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACa                                             <  2:3084645/149‑1 (MQ=255)
                                                                                                      cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACa                                             <  2:296008/149‑1 (MQ=255)
                                                                                                      cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACa                                             <  1:71715/149‑1 (MQ=255)
                                                                                                       aTAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAt                                            >  1:211982/1‑149 (MQ=255)
                                                                                                       aTAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAt                                            <  2:1477438/149‑1 (MQ=255)
                                                                                                       aTAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAt                                            <  1:3793709/149‑1 (MQ=255)
                                                                                                         aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATAc                                          >  1:3275706/1‑149 (MQ=255)
                                                                                                                 cgacaacaGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTTACCGTTGATGAAAGCACCTGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGc                                  <  1:3170834/143‑1 (MQ=255)
                                                                                                                 cGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGc                                  <  2:2126683/149‑1 (MQ=255)
                                                                                                                  gCCACCATCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGcc                                 <  2:3409509/149‑1 (MQ=255)
                                                                                                                    caccaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCgg                               <  1:834365/149‑1 (MQ=255)
                                                                                                                     accaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCggg                              >  2:2033525/1‑149 (MQ=255)
                                                                                                                     accaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCggg                              <  1:551405/149‑1 (MQ=255)
                                                                                                                      ccaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGt                             >  2:2148033/1‑149 (MQ=255)
                                                                                                                       caGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTa                            <  1:2895388/149‑1 (MQ=255)
                                                                                                                        aGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTAt                           <  1:1273664/149‑1 (MQ=255)
                                                                                                                        aGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTAt                           >  1:2364474/1‑149 (MQ=255)
                                                                                                                         gCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATg                          >  1:3097324/1‑149 (MQ=255)
                                                                                                                         gCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATg                          >  2:2548021/1‑149 (MQ=255)
                                                                                                                          cAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGt                         >  2:3666864/1‑149 (MQ=255)
                                                                                                                           aaCGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGtt                        <  2:1984371/149‑1 (MQ=255)
                                                                                                                            aCGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGttt                       >  2:2408535/1‑149 (MQ=255)
                                                                                                                              gTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTtgt                     >  2:3852497/1‑149 (MQ=255)
                                                                                                                               tNGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGNAAGGTACGCTGCAATTCTCCGATGTGACCNTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTNACCCGCAACATACGCTTTTGCCGGGTATGTTtgtg                    >  1:600187/1‑149 (MQ=255)
                                                                                                                                   gTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgtg                >  2:4061248/1‑149 (MQ=255)
                                                                                                                                      ggTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCAc             <  2:2480525/149‑1 (MQ=255)
                                                                                                                                       gTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACg            >  2:2339172/1‑149 (MQ=255)
                                                                                                                                       gTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACg            >  2:3351806/1‑149 (MQ=255)
                                                                                                                                       gTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACg            >  2:1302110/1‑149 (MQ=255)
                                                                                                                                        tCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACgg           >  1:1554457/1‑149 (MQ=255)
                                                                                                                                         cATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGa          >  1:2624458/1‑149 (MQ=255)
                                                                                                                                               aaaaGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtgatg    >  2:19522/1‑149 (MQ=255)
                                                                                                                                                 aCGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGNACCGGCNCCATNACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATNaa  >  2:1396975/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAA  >  CP009273/3407823‑3408116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑