Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,424,363 T→A 100% T138S (ACC→TCC)  tsaC ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,424,3630TA96.6% 855.7 / 12.0 263T138S (ACC→TCC) tsaCtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base A (128/124);  total (133/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

CAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGC  >  CP009273/3424217‑3424508
                                                                                                                                                  |                                                                                                                                                 
cAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAg                                                                                                                                                 >  1:1839998/1‑149 (MQ=255)
 aGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGa                                                                                                                                                >  1:3072554/1‑149 (MQ=255)
  gtacACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                               <  1:3448476/146‑1 (MQ=255)
     cACCCGTCTGGGCAGGGGGTATTTCTTAAGTATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAAcc                                                                                                                                            <  1:352990/149‑1 (MQ=255)
      aCCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCa                                                                                                                                           <  1:892182/149‑1 (MQ=255)
        ccGTCTGGGCAACGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGc                                                                                                                                         <  2:3097115/149‑1 (MQ=255)
         cGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAGCCAGCg                                                                                                                                        <  2:1222322/149‑1 (MQ=255)
         cGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCg                                                                                                                                        >  1:4043127/1‑149 (MQ=255)
               ggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTAc                                                                                                                                  <  1:337868/149‑1 (MQ=255)
                     gcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAg                                                                                                                            >  2:3872478/1‑149 (MQ=255)
                      cgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGc                                                                                                                           <  2:85285/149‑1 (MQ=255)
                      cgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGc                                                                                                                           <  2:2448209/149‑1 (MQ=255)
                       ggATTTCTGAAGGATAAAAACGCCCCCCCGTAACACCACCCACAACCGGGAACGCCCCGCCAAACACAGCGCGAACATCCTCTACAGTTCCACAACGTGGCAAACCACTCAACTTGGCACTGCAACAAACCAGCGGTTTACCATAAGcc                                                                                                                          <  2:505307/149‑1 (MQ=255)
                        gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCACTCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                         >  1:485141/1‑149 (MQ=255)
                        gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                         >  1:2155124/1‑149 (MQ=255)
                        gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                         >  2:1931556/1‑149 (MQ=255)
                        gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                         >  2:1099372/1‑149 (MQ=255)
                         aTTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTg                                                                                                                        >  2:3537362/1‑149 (MQ=255)
                         aTTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTg                                                                                                                        >  1:2878800/1‑149 (MQ=255)
                          tttCTGAAGTATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTgg                                                                                                                       <  1:3174412/149‑1 (MQ=255)
                          tttCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTgg                                                                                                                       <  1:452826/149‑1 (MQ=255)
                          tttCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTgg                                                                                                                       >  2:2235906/1‑149 (MQ=255)
                           ttCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGc                                                                                                                      >  2:3484909/1‑149 (MQ=255)
                              tGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGcaca                                                                                                                   >  2:3197323/1‑149 (MQ=255)
                              tGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGcaca                                                                                                                   >  2:1791792/1‑149 (MQ=255)
                               gAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                  >  2:3139234/1‑149 (MQ=255)
                                 aGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAg                                                                                                                <  2:3447105/149‑1 (MQ=255)
                                     tttAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAac                                                                                                            >  2:1158055/1‑149 (MQ=255)
                                     tttAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAac                                                                                                            <  1:416527/149‑1 (MQ=255)
                                      ttACACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAacc                                                                                                           <  1:2420483/149‑1 (MQ=255)
                                      ttAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAacc                                                                                                           <  1:1301804/149‑1 (MQ=255)
                                         aaCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccacc                                                                                                        <  2:81573/149‑1 (MQ=255)
                                          aCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAccacca                                                                                                       <  2:2552011/149‑1 (MQ=255)
                                           cGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCaa                                                                                                      <  2:3646693/149‑1 (MQ=255)
                                            gCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAAc                                                                                                     >  1:26185/1‑149 (MQ=255)
                                            gCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAAc                                                                                                     <  1:2658758/149‑1 (MQ=255)
                                            gCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAAc                                                                                                     <  1:3904060/149‑1 (MQ=255)
                                             cccccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAACACCAACg                                                                                                    >  1:3251822/1‑149 (MQ=255)
                                                ccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGAt                                                                                                 >  1:2809506/1‑149 (MQ=255)
                                                  ccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATgg                                                                                               <  1:3970889/149‑1 (MQ=255)
                                                  ccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTGTACCATAAGCCTGGCACAAATCAACCACCAACGGATgg                                                                                               >  1:3713098/1‑149 (MQ=255)
                                                   cGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGt                                                                                              <  1:88772/149‑1 (MQ=255)
                                                   cGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGt                                                                                              <  1:3703758/149‑1 (MQ=255)
                                                   cGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGt                                                                                              <  2:3001721/149‑1 (MQ=255)
                                                    gTTTCTCCAGGCACAACCGTGAACGCCTAGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACATGGTGGCAATCCACTCTATTTGTCACTGGAAGAAACCAGCGGTTTACCATCAGCCTGGCACAAAGCAACCACCAACGGATGGTc                                                                                             <  2:2600062/149‑1 (MQ=255)
                                                    gTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTc                                                                                             >  2:3632105/1‑149 (MQ=255)
                                                    gTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTc                                                                                             >  1:2478908/1‑149 (MQ=255)
                                                     tttCACCAGACACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCg                                                                                            >  1:204645/1‑149 (MQ=255)
                                                      ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCgg                                                                                           <  1:1890825/149‑1 (MQ=255)
                                                      ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCgg                                                                                           <  1:1040719/149‑1 (MQ=255)
                                                            aGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTc                                                                                     >  2:428453/1‑149 (MQ=255)
                                                             ggCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                    >  2:752593/1‑149 (MQ=255)
                                                                  aaCCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCCAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAg                                                                               >  2:107624/1‑149 (MQ=255)
                                                                     cGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCaa                                                                            <  2:2786586/149‑1 (MQ=255)
                                                                     cGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCaa                                                                            >  2:661330/1‑149 (MQ=255)
                                                                       ggAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGc                                                                          >  1:770114/1‑149 (MQ=255)
                                                                       ggAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGc                                                                          >  1:2830492/1‑149 (MQ=255)
                                                                            gccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATc                                                                     <  2:831455/149‑1 (MQ=255)
                                                                             ccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCa                                                                    <  1:2181209/149‑1 (MQ=255)
                                                                              cgcgCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCaa                                                                   <  2:1751123/149‑1 (MQ=255)
                                                                                    aaaTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGc                                                             >  2:3902044/1‑149 (MQ=255)
                                                                                     aaTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCACGCGAATCAAAGCGGcc                                                            >  2:2150033/1‑149 (MQ=255)
                                                                                     aaTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGAAATCAACTCATGTTGGCACTGGAAGAAACCTGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGTTGGTCGGTTACTCGTACAGCAAGCGAATCAAAGCGGcc                                                            <  1:3405790/149‑1 (MQ=255)
                                                                                       ttGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCg                                                          >  1:3209722/1‑149 (MQ=255)
                                                                                        tgtgCGCGAACTTCGTCTACTGTTCGACAAGGTGGAAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGTTTTACCATAAGCCTGGCACAAAGAAACCACAAACGGATGGTGGGTGACTCGTACAGCAAGCTAATCAAAGCGGCCCGt                                                         <  1:661396/149‑1 (MQ=255)
                                                                                           gcgcGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCaa                                                      >  1:3860796/1‑149 (MQ=255)
                                                                                               gAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAg                                                  >  2:290912/1‑149 (MQ=255)
                                                                                                 aCTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAgcg                                                >  2:3103028/1‑149 (MQ=255)
                                                                                                       tCTACTGTTCGACAAGGTGGCAATCCACTCACGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtgt                                          <  2:3275861/149‑1 (MQ=255)
                                                                                                       tCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGTGAATCAAAGCGGCCCGTCAACCAGCGCGgtgt                                          <  2:3663295/149‑1 (MQ=255)
                                                                                                         tACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttg                                        >  1:2000202/1‑149 (MQ=255)
                                                                                                          aCTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttgt                                       >  2:2722892/1‑149 (MQ=255)
                                                                                                             tttCGACATGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGGGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGTTGGTCGTTGACTCGTACAGAAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGc                                    <  1:2899890/148‑1 (MQ=255)
                                                                                                             gTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGc                                    >  2:670219/1‑149 (MQ=255)
                                                                                                              ttCGACATGTGGGCAATCAACTAAAGTTGGCACTGTAAGAAACCAGGGGTTTACAATACGCCTGGCACAAAGAAACCACCACCGTTTGGTCGGTGACTCTTACAGAAAGAGAATAAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCa                                   <  1:1255983/149‑1 (MQ=255)
                                                                                                               tCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAg                                  <  2:3701262/149‑1 (MQ=255)
                                                                                                               tCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAg                                  >  1:1874980/1‑149 (MQ=255)
                                                                                                               tCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAg                                  <  2:4030570/149‑1 (MQ=255)
                                                                                                                  aCAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGgcg                               >  2:549536/1‑149 (MQ=255)
                                                                                                                   cAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGgcgc                              <  1:2547390/149‑1 (MQ=255)
                                                                                                                     aGGTGGAAATCCACTCCCGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCACGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCgg                            <  2:2233494/149‑1 (MQ=255)
                                                                                                                      ggTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCggg                           <  2:2411347/149‑1 (MQ=255)
                                                                                                                        tGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaa                         >  2:1389530/1‑149 (MQ=255)
                                                                                                                         gtcAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATAAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaaa                        <  2:444344/147‑1 (MQ=255)
                                                                                                                         ggCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaaa                        <  1:1338984/149‑1 (MQ=255)
                                                                                                                         ggCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaaa                        >  2:234501/1‑149 (MQ=255)
                                                                                                                             aTCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACa                    >  1:2620787/1‑149 (MQ=255)
                                                                                                                              tCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACaa                   <  2:1818271/149‑1 (MQ=255)
                                                                                                                               ccACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACaaa                  <  1:2294928/149‑1 (MQ=255)
                                                                                                                                cACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAg                 <  2:1312921/149‑1 (MQ=255)
                                                                                                                                    cAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGa             <  1:3621890/149‑1 (MQ=255)
                                                                                                                                     aaGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGAc            >  1:1775753/1‑149 (MQ=255)
                                                                                                                                       gTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAg          >  1:2167769/1‑149 (MQ=255)
                                                                                                                                        ttGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAgg         >  1:1700758/1‑149 (MQ=255)
                                                                                                                                         tGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGa        <  1:1249163/149‑1 (MQ=255)
                                                                                                                                          ggCACTGGAAGAAACCAGCGGTTTACCATCAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAc       <  1:3504633/149‑1 (MQ=255)
                                                                                                                                           gCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAcc      <  2:3758766/149‑1 (MQ=255)
                                                                                                                                            cACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCt     <  2:2088086/149‑1 (MQ=255)
                                                                                                                                             aCTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTg    >  2:1597920/1‑149 (MQ=255)
                                                                                                                                               tGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGc  >  1:4042957/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGC  >  CP009273/3424217‑3424508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑