Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,445,410 C→A 100% R79L (CGT→CTT)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,445,4100CA99.1% 718.1 / ‑2.0 218R79L (CGT→CTT) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (118/98);  total (118/100)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

CTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACC  >  CP009273/3445264‑3445556
                                                                                                                                                  |                                                                                                                                                  
cTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGc                                                                                                                                                  >  1:2903152/1‑149 (MQ=255)
cTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGc                                                                                                                                                  <  1:2725043/149‑1 (MQ=255)
cTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGc                                                                                                                                                  >  1:398845/1‑149 (MQ=255)
 ttCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGcc                                                                                                                                                 <  2:2590990/149‑1 (MQ=255)
 ttCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGcc                                                                                                                                                 <  1:143623/149‑1 (MQ=255)
     aCAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGAt                                                                                                                                             <  1:2118834/149‑1 (MQ=255)
     aCAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGAt                                                                                                                                             >  1:1422809/1‑149 (MQ=255)
     aCAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGAt                                                                                                                                             <  1:1080739/149‑1 (MQ=255)
       agagAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCg                                                                                                                                           <  2:2419334/149‑1 (MQ=255)
        gagaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCgg                                                                                                                                          <  2:4079081/149‑1 (MQ=255)
         agaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCggg                                                                                                                                         >  1:2677046/1‑149 (MQ=255)
          gaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGc                                                                                                                                        >  2:3733883/1‑149 (MQ=255)
             cTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctct                                                                                                                                     >  2:3845397/1‑149 (MQ=255)
             cTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctct                                                                                                                                     <  1:729554/149‑1 (MQ=255)
                    aCAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGa                                                                                                                              >  1:516314/1‑149 (MQ=255)
                      aaCGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAc                                                                                                                            >  1:2257689/1‑149 (MQ=255)
                         gATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAg                                                                                                                         >  1:3355467/1‑149 (MQ=255)
                           tCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGaa                                                                                                                       <  1:1025648/149‑1 (MQ=255)
                           tCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGaa                                                                                                                       >  1:3381137/1‑149 (MQ=255)
                           tCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGaa                                                                                                                       <  2:1853048/149‑1 (MQ=255)
                              gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgc                                                                                                                    >  2:1960958/1‑149 (MQ=255)
                              gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgc                                                                                                                    >  2:2128550/1‑149 (MQ=255)
                              gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgc                                                                                                                    >  2:576750/1‑149 (MQ=255)
                                  aTCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  1:878540/1‑149 (MQ=255)
                                        aCGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCgg                                                                                                          >  1:1045804/1‑149 (MQ=255)
                                        aCGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCgg                                                                                                          <  2:1117449/149‑1 (MQ=255)
                                         cGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGt                                                                                                         <  1:2447551/149‑1 (MQ=255)
                                          gTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                                                                        >  1:2066799/1‑149 (MQ=255)
                                          gTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                                                                        <  2:588176/149‑1 (MQ=255)
                                            aCCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                                                                      <  2:819273/149‑1 (MQ=255)
                                             ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCt                                                                                                     >  1:795956/1‑149 (MQ=255)
                                             ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCt                                                                                                     <  1:175424/149‑1 (MQ=255)
                                             ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCt                                                                                                     >  1:3885367/1‑149 (MQ=255)
                                              cAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAAGCGCACGGCCGGTGCCtt                                                                                                    <  1:2039270/149‑1 (MQ=255)
                                                gTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTTCATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGTTAGAACCAGAACGCGCACGGCCGGTGCCTTTc                                                                                                  <  2:1615691/149‑1 (MQ=255)
                                                gTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTc                                                                                                  <  1:270457/149‑1 (MQ=255)
                                                     gACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                                                             >  1:3456339/1‑149 (MQ=255)
                                                       cAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                                                                           <  1:3330705/149‑1 (MQ=255)
                                                        aGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCa                                                                                          <  2:3969959/149‑1 (MQ=255)
                                                         ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                                                                         >  1:3767771/1‑149 (MQ=255)
                                                         ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                                                                         >  1:607803/1‑149 (MQ=255)
                                                         ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                                                                         >  2:1748101/1‑149 (MQ=255)
                                                          gATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACg                                                                                        >  2:2999605/1‑149 (MQ=255)
                                                          gATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACg                                                                                        >  2:2172248/1‑149 (MQ=255)
                                                            tGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGt                                                                                      >  2:2516952/1‑149 (MQ=255)
                                                            tGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGt                                                                                      <  2:1974038/149‑1 (MQ=255)
                                                             gCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtt                                                                                     <  2:1846958/149‑1 (MQ=255)
                                                                tttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttttt                                                                                  <  2:2547383/149‑1 (MQ=255)
                                                                  tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTa                                                                                >  1:3574760/1‑149 (MQ=255)
                                                                  tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTa                                                                                >  1:1525213/1‑149 (MQ=255)
                                                                       gcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGa                                                                           >  1:2555695/1‑149 (MQ=255)
                                                                       gcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGa                                                                           >  1:1899759/1‑149 (MQ=255)
                                                                            gcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                                                                      <  2:152521/149‑1 (MQ=255)
                                                                            gcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                                                                      <  2:1678747/149‑1 (MQ=255)
                                                                             cgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                                                                     <  1:3425363/149‑1 (MQ=255)
                                                                             cgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                                                                     <  1:313403/149‑1 (MQ=255)
                                                                               gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTa                                                                   >  2:354064/1‑149 (MQ=255)
                                                                               gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTa                                                                   <  1:1514356/149‑1 (MQ=255)
                                                                               gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTa                                                                   >  1:1127739/1‑149 (MQ=255)
                                                                               gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTa                                                                   >  1:562000/1‑149 (MQ=255)
                                                                                tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                                                                  >  1:989004/1‑149 (MQ=255)
                                                                                  cATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACtt                                                                >  1:475016/1‑149 (MQ=255)
                                                                                   aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                                                               >  1:2429188/1‑149 (MQ=255)
                                                                                   aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                                                               >  2:178805/1‑149 (MQ=255)
                                                                                       tcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa                                                           <  1:2128550/149‑1 (MQ=255)
                                                                                           gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg                                                       <  1:3550441/149‑1 (MQ=255)
                                                                                           gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg                                                       <  2:2200711/149‑1 (MQ=255)
                                                                                            ttAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGt                                                      >  2:2806546/1‑149 (MQ=255)
                                                                                            ttAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGt                                                      >  2:2859792/1‑149 (MQ=255)
                                                                                            ttAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGt                                                      <  1:757695/149‑1 (MQ=255)
                                                                                             tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc                                                     <  2:3096398/149‑1 (MQ=255)
                                                                                             tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc                                                     <  1:2026592/149‑1 (MQ=255)
                                                                                               aCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtctt                                                   >  2:2667549/1‑149 (MQ=255)
                                                                                               aCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtctt                                                   >  2:3419722/1‑149 (MQ=255)
                                                                                                  ttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg                                                >  2:1934286/1‑149 (MQ=255)
                                                                                                           tgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGt                                       >  1:2377677/1‑149 (MQ=255)
                                                                                                              tCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAcc                                    >  2:1307529/1‑149 (MQ=255)
                                                                                                                  gCGGACGATCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGa                                <  2:2441075/149‑1 (MQ=255)
                                                                                                                   cGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGAc                               <  2:7470/149‑1 (MQ=255)
                                                                                                                    ggACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACg                              >  1:2226033/1‑149 (MQ=255)
                                                                                                                    ggACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACg                              >  2:3391012/1‑149 (MQ=255)
                                                                                                                     gACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGa                             >  2:3769563/1‑149 (MQ=255)
                                                                                                                       cGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGc                           <  1:224635/149‑1 (MQ=255)
                                                                                                                         agcagcAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCAc                         <  1:4058928/149‑1 (MQ=255)
                                                                                                                          gcagcaAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCAcc                        <  2:1228435/149‑1 (MQ=255)
                                                                                                                          gcagcaAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCAcc                        <  2:1399664/149‑1 (MQ=255)
                                                                                                                             gcaAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGc                     <  1:1637906/149‑1 (MQ=255)
                                                                                                                             gcaAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCATGACGAGCACCAGc                     >  1:3443781/1‑149 (MQ=255)
                                                                                                                                aaGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGc                  >  1:1844721/1‑149 (MQ=255)
                                                                                                                                 aGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCa                 <  2:1000466/149‑1 (MQ=255)
                                                                                                                                 aGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCa                 >  2:3800296/1‑149 (MQ=255)
                                                                                                                                  ggTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCAt                <  2:1764512/149‑1 (MQ=255)
                                                                                                                                         ccaccaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaac         >  2:1450672/1‑149 (MQ=255)
                                                                                                                                         ccaccaGAAAGCCAGATAGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaac         <  2:1422809/149‑1 (MQ=255)
                                                                                                                                           accaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaa       <  2:139280/149‑1 (MQ=255)
                                                                                                                                            ccaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaac      >  2:649048/1‑149 (MQ=255)
                                                                                                                                             caGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaaca     <  1:2516952/149‑1 (MQ=255)
                                                                                                                                               gAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCaacaacaac   <  1:831777/149‑1 (MQ=255)
                                                                                                                                               gAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCaacaacaac   <  1:2891094/149‑1 (MQ=255)
                                                                                                                                                aaCGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAAcc  <  2:304601/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACC  >  CP009273/3445264‑3445556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑