Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,448,742 C→A 100% E6* (GAA→TAA)  gspA ← general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,448,7420CA98.2% 714.3 / 2.9 224E6* (GAA→TAA) gspAgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (72/148);  total (73/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

AACAACGAGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTCTCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATACTATTTATA  >  CP009273/3448596‑3448887
                                                                                                                                                  |                                                                                                                                                 
aacaacGAGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAActtatc                                                                                                                                                 >  2:838371/1‑149 (MQ=255)
aacaacGAGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAActtatc                                                                                                                                                 <  2:3044977/149‑1 (MQ=255)
aacaacGAGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAActtatc                                                                                                                                                 <  2:1477531/149‑1 (MQ=255)
    acGAGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAActtatcttct                                                                                                                                             <  2:3721981/149‑1 (MQ=255)
    acGAGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAActtatcttct                                                                                                                                             >  1:1653343/1‑149 (MQ=255)
       aGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGt                                                                                                                                          <  2:1667300/149‑1 (MQ=255)
         gCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAg                                                                                                                                        <  1:2271256/149‑1 (MQ=255)
          cTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGa                                                                                                                                       <  1:944292/149‑1 (MQ=255)
          cTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGa                                                                                                                                       <  1:2291670/149‑1 (MQ=255)
           ttAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGAc                                                                                                                                      <  2:1755683/149‑1 (MQ=255)
            tAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACa                                                                                                                                     <  1:1224084/149‑1 (MQ=255)
            tAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACa                                                                                                                                     <  2:293656/149‑1 (MQ=255)
            tAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACa                                                                                                                                     >  1:432279/1‑149 (MQ=255)
              aaGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATa                                                                                                                                   >  1:176804/1‑149 (MQ=255)
              aaGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATa                                                                                                                                   >  1:176862/1‑149 (MQ=255)
                  aaTCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAAc                                                                                                                               >  2:3531746/1‑149 (MQ=255)
                  aaTCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAAc                                                                                                                               <  1:3712856/149‑1 (MQ=255)
                    tCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACtt                                                                                                                             <  1:2117368/149‑1 (MQ=255)
                     cacaCGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTc                                                                                                                            <  1:1488535/149‑1 (MQ=255)
                      acacGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTcc                                                                                                                           <  2:1553912/149‑1 (MQ=255)
                      acacGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTcc                                                                                                                           <  2:728361/149‑1 (MQ=255)
                      acacGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTcc                                                                                                                           <  2:1794508/149‑1 (MQ=255)
                       tacGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCt                                                                                                                          <  1:69777/148‑1 (MQ=255)
                       cacGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCt                                                                                                                          >  2:257735/1‑149 (MQ=255)
                            cGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGtttt                                                                                                                     <  2:2736540/149‑1 (MQ=255)
                              aTGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTaa                                                                                                                   >  2:3164513/1‑149 (MQ=255)
                               tGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAat                                                                                                                  <  1:3372548/149‑1 (MQ=255)
                                gTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTCTCTTCTCGTAGACATAGAACTTCCTGTTTTAatt                                                                                                                 <  2:3920997/149‑1 (MQ=255)
                                gTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCAATTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAatt                                                                                                                 <  1:4022375/149‑1 (MQ=255)
                                 tttATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAatta                                                                                                                >  1:3428307/1‑149 (MQ=255)
                                 tttATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAatta                                                                                                                <  1:2368963/149‑1 (MQ=255)
                                    aTCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTg                                                                                                             <  2:3340247/149‑1 (MQ=255)
                                     tCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGa                                                                                                            >  1:3296499/1‑149 (MQ=255)
                                     tCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGa                                                                                                            >  2:589359/1‑149 (MQ=255)
                                     tCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGa                                                                                                            >  1:3013205/1‑149 (MQ=255)
                                      cTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGAt                                                                                                           >  2:3417749/1‑149 (MQ=255)
                                       tttttGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCTGCCTCACCCAACAAATAGCATCGACGCCATGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGAtt                                                                                                          <  1:3211495/149‑1 (MQ=255)
                                        ttttGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTa                                                                                                         <  2:2634279/149‑1 (MQ=255)
                                          ttGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATc                                                                                                       <  2:833027/149‑1 (MQ=255)
                                          ttGCAGGTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATc                                                                                                       <  2:2895270/149‑1 (MQ=255)
                                              aGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTa                                                                                                   >  1:1545665/1‑149 (MQ=255)
                                               gTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTaa                                                                                                  <  2:1915208/149‑1 (MQ=255)
                                               gTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAAGAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTaa                                                                                                  <  2:2192150/149‑1 (MQ=255)
                                                 tgctgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATc                                                                                                >  1:240282/1‑149 (MQ=255)
                                                  gctgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCa                                                                                               <  2:2860522/149‑1 (MQ=255)
                                                  gctgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCa                                                                                               <  2:402654/149‑1 (MQ=255)
                                                   ctgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTCTCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCAt                                                                                              <  2:2428011/149‑1 (MQ=255)
                                                   ctgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCAt                                                                                              <  2:3777133/149‑1 (MQ=255)
                                                   ctgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCAt                                                                                              <  2:1463563/149‑1 (MQ=255)
                                                    tgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATa                                                                                             <  1:3856593/149‑1 (MQ=255)
                                                    tgcGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATa                                                                                             <  2:1175754/149‑1 (MQ=255)
                                                     gcgcCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATaa                                                                                            >  1:413651/1‑149 (MQ=255)
                                                     gcgcCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATaa                                                                                            <  1:4070637/149‑1 (MQ=255)
                                                     gcgcCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATaa                                                                                            <  1:2833363/149‑1 (MQ=255)
                                                      cgcCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATCGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATaaa                                                                                           <  1:3793380/149‑1 (MQ=255)
                                                         cagccagGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAaat                                                                                        <  2:3429834/149‑1 (MQ=255)
                                                          agccagGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAaata                                                                                       <  2:1340513/149‑1 (MQ=255)
                                                          agccagGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAaata                                                                                       <  2:3775953/149‑1 (MQ=255)
                                                           gccagGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAaataa                                                                                      <  2:2795648/149‑1 (MQ=255)
                                                           gccagGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAaataa                                                                                      <  2:2077256/149‑1 (MQ=255)
                                                            ccagTTTTTTCCACTTCCAGCCTCACCCAACAAATAGCAGAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAaataat                                                                                     <  1:1739769/149‑1 (MQ=255)
                                                                    ttCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATgtatgtat                                                                             <  1:1899213/149‑1 (MQ=255)
                                                                    ttCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATgtatgtat                                                                             <  2:2077335/149‑1 (MQ=255)
                                                                    ttCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATgtatgtat                                                                             <  2:4109320/149‑1 (MQ=255)
                                                                     tCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGtatt                                                                            <  2:176862/149‑1 (MQ=255)
                                                                        aCTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGtatttat                                                                         <  2:1543044/149‑1 (MQ=255)
                                                                         cTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGtatttatt                                                                        <  2:775923/149‑1 (MQ=255)
                                                                            ccAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTattaat                                                                     <  1:600111/149‑1 (MQ=255)
                                                                                ccTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTGTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTaaa                                                                 <  1:2616174/149‑1 (MQ=255)
                                                                                     cccAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACat                                                            <  1:3417772/149‑1 (MQ=255)
                                                                                      ccAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACata                                                           <  1:838371/149‑1 (MQ=255)
                                                                                         acaaATAGCCTAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTa                                                        <  1:2739275/149‑1 (MQ=255)
                                                                                         acaaATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTa                                                        <  1:1895125/149‑1 (MQ=255)
                                                                                           aaaTAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACt                                                      <  1:851550/149‑1 (MQ=255)
                                                                                             aTAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTaa                                                    <  2:1707431/149‑1 (MQ=255)
                                                                                             aTAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTaa                                                    <  1:2952319/149‑1 (MQ=255)
                                                                                               aGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAata                                                  <  1:1071385/149‑1 (MQ=255)
                                                                                                    aGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATataaat                                             >  1:13479/1‑149 (MQ=255)
                                                                                                     gACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATataaata                                            >  2:3218334/1‑149 (MQ=255)
                                                                                                       cGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATAtaaataaa                                          <  2:70903/149‑1 (MQ=255)
                                                                                                       cGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATAtaaataaa                                          >  1:2660075/1‑149 (MQ=255)
                                                                                                           aGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAAtttt                                      >  2:2671264/1‑149 (MQ=255)
                                                                                                             gTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGc                                    <  1:3371753/149‑1 (MQ=255)
                                                                                                              tttGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCt                                   <  1:3543323/149‑1 (MQ=255)
                                                                                                               ttGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCtt                                  <  1:320640/149‑1 (MQ=255)
                                                                                                               ttGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCtt                                  >  2:3799515/1‑149 (MQ=255)
                                                                                                                tGACGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTg                                 >  2:3693622/1‑149 (MQ=255)
                                                                                                                  aCGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGAt                               >  1:836507/1‑149 (MQ=255)
                                                                                                                  aCGTTTCTCACACAACCAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGAt                               >  2:416951/1‑149 (MQ=255)
                                                                                                                                 ccAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCAt                <  2:1749354/149‑1 (MQ=255)
                                                                                                                                 ccAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCAt                <  1:3692211/149‑1 (MQ=255)
                                                                                                                                 ccAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCAt                <  2:3790796/149‑1 (MQ=255)
                                                                                                                                 ccAGGAAAGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCAt                <  1:880290/149‑1 (MQ=255)
                                                                                                                                      aaaGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATa           <  1:2671264/149‑1 (MQ=255)
                                                                                                                                       aaGAATAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATAc          <  1:1305641/149‑1 (MQ=255)
                                                                                                                                          aaTAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATACTAt       >  2:566468/1‑149 (MQ=255)
                                                                                                                                          aaTAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGAAAGCGGCATTAATACTAt       >  2:1523864/1‑149 (MQ=255)
                                                                                                                                           aTAACTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATACTAtt      <  1:439171/149‑1 (MQ=255)
                                                                                                                                             aaCTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATACTATTta    <  2:3820896/149‑1 (MQ=255)
                                                                                                                                             aaCTTATCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATACTATTta    <  2:3243762/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACAACGAGGCTTAAAGTAATCACACGGCGATGTTTATCTTTTTGCAGTTGCTGCGCCAGCCAGGTTTTTCCACTTCCAGCCTCACCCAACAAATAGCATAGACGCCAGGTTTGACGTTTCTCACACAACCAGGAAAGAATAACTTCTCTTCTCGTAGACATAGAACTTCCTGTTTTAATTATTGATTATCCTTAATCATAAAAATAATGTATGTATTTATTAATTAAATACATATTACTAATATAAATAAATTTTGCTTGATTCATGCAAGCGGCATTAATACTATTTATA  >  CP009273/3448596‑3448887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑