Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,455,867 C→A 100% I55I (ATC→ATA)  gspJ → putative general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,455,8670CA100.0% 745.7 / ‑6.7 225I55I (ATC→ATA) gspJputative general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (85/139);  total (85/140)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

GGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACT  >  CP009273/3455721‑3456010
                                                                                                                                                  |                                                                                                                                               
ggTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataac                                                                                                                                               <  1:1268678/149‑1 (MQ=255)
ggTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataac                                                                                                                                               <  1:3826779/149‑1 (MQ=255)
 gTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataacc                                                                                                                                              <  1:720475/149‑1 (MQ=255)
    tCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCAtt                                                                                                                                           <  1:2412206/149‑1 (MQ=255)
     cacaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTc                                                                                                                                          <  2:3298234/149‑1 (MQ=255)
      acaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCt                                                                                                                                         <  1:1049435/149‑1 (MQ=255)
       caTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCtt                                                                                                                                        >  2:273397/1‑149 (MQ=255)
        aTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTg                                                                                                                                       <  2:2052499/149‑1 (MQ=255)
         ttGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGa                                                                                                                                      <  2:1226399/149‑1 (MQ=255)
         ttGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGa                                                                                                                                      <  1:1700536/149‑1 (MQ=255)
          tGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGAt                                                                                                                                     <  2:2691188/149‑1 (MQ=255)
            cTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATaa                                                                                                                                   <  2:1469140/149‑1 (MQ=255)
             tGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAAc                                                                                                                                  <  1:377940/149‑1 (MQ=255)
                 ggTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGAtc                                                                                                                              <  1:1655742/149‑1 (MQ=255)
                    cATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTcc                                                                                                                           <  2:941748/149‑1 (MQ=255)
                     aTGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCt                                                                                                                          <  1:939711/149‑1 (MQ=255)
                     aTGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCt                                                                                                                          <  1:4090126/149‑1 (MQ=255)
                     aTGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCt                                                                                                                          <  1:3956464/149‑1 (MQ=255)
                      tGGCGGCGCTGTCGATTTTCTCAATGCATAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGATAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCCTTCTGGATAACGATCTCCTg                                                                                                                         <  2:184546/149‑1 (MQ=255)
                       ggcggcGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGc                                                                                                                        <  1:888856/149‑1 (MQ=255)
                             gcTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTgg                                                                                                                  <  1:165048/149‑1 (MQ=255)
                               tGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTAGCAAGCTTCCGAGTTGCATCAAAGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCAAAACCATTCTTGATAACGATCTCCTGCAACTGGTc                                                                                                                >  1:2034184/1‑149 (MQ=255)
                               tGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTGTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCACCTGGTc                                                                                                                >  2:142108/1‑149 (MQ=255)
                                      tttCTCAATGCTTAGCGTGCTGGCGTGTATGATTTTTTAGCAAGCTTCCGAGTTTCAGCAAAGCTCACAGAAAGAAATACAGCAGTTCAAAAACCGACAGCCCACCAAAAACCATATTTTTAAAGATCACCAGCAAAAGGTCGCCCcga                                                                                                         >  1:2454752/1‑149 (MQ=255)
                                         ctcAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCggagga                                                                                                      <  1:3503450/149‑1 (MQ=255)
                                         ctcAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCggagga                                                                                                      <  1:2678442/149‑1 (MQ=255)
                                         ctcAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCggagga                                                                                                      <  1:3274359/149‑1 (MQ=255)
                                           cAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAAt                                                                                                    <  1:148297/149‑1 (MQ=255)
                                             aTGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCg                                                                                                  <  1:1898393/149‑1 (MQ=255)
                                                cTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAg                                                                                               <  2:2905758/149‑1 (MQ=255)
                                                cTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAg                                                                                               <  2:319820/149‑1 (MQ=255)
                                                  tAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTa                                                                                             <  1:362622/149‑1 (MQ=255)
                                                   agagTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTAACAGAAAGAAAACAAGCAGTTCAATAAGCTACAGCGCACCATAACAAATCTTTATAACGATATCCTGCAACTGGTCGCCCGGAGGAATCGGAGTaa                                                                                            >  2:2608801/4‑148 (MQ=255)
                                                          tGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaa                                                                                     >  2:2151360/1‑149 (MQ=255)
                                                           ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                    >  2:729572/1‑149 (MQ=255)
                                                           ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                    <  2:452241/149‑1 (MQ=255)
                                                           ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                    <  2:41042/149‑1 (MQ=255)
                                                              gTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAAtaa                                                                                 <  1:366622/149‑3 (MQ=255)
                                                                ttATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATg                                                                               >  1:3310615/1‑149 (MQ=255)
                                                                 tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATgg                                                                              <  2:2790146/149‑1 (MQ=255)
                                                                 tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATgg                                                                              <  2:797420/149‑1 (MQ=255)
                                                                  aTGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgt                                                                             >  1:1002904/1‑149 (MQ=255)
                                                                  aTGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgt                                                                             <  2:1570673/149‑1 (MQ=255)
                                                                      ttttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTg                                                                         <  1:2290369/149‑1 (MQ=255)
                                                                           tCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTga                                                                    <  1:63148/149‑1 (MQ=255)
                                                                            cGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaa                                                                   <  1:1179280/149‑1 (MQ=255)
                                                                            cGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaa                                                                   <  1:2468848/149‑1 (MQ=255)
                                                                            cGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaa                                                                   <  1:1179511/149‑1 (MQ=255)
                                                                             gCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaag                                                                  >  2:840398/1‑149 (MQ=255)
                                                                               aaGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaagaa                                                                >  2:2804624/1‑149 (MQ=255)
                                                                                aGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGaagaag                                                               >  1:180495/1‑149 (MQ=255)
                                                                                 gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGc                                                              >  2:1597294/1‑149 (MQ=255)
                                                                                 gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGc                                                              >  2:783403/1‑149 (MQ=255)
                                                                                   ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTa                                                            <  2:1676386/149‑1 (MQ=255)
                                                                                     ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtt                                                          <  1:370746/149‑1 (MQ=255)
                                                                                       gAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtttt                                                        >  2:3041651/1‑149 (MQ=255)
                                                                                         gTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTa                                                      <  2:1095205/149‑1 (MQ=255)
                                                                                         gTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTa                                                      <  2:2391290/149‑1 (MQ=255)
                                                                                          ttGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAc                                                     >  2:4074643/1‑149 (MQ=255)
                                                                                          ttGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAc                                                     <  2:3100185/149‑1 (MQ=255)
                                                                                            gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCa                                                   <  2:2343351/149‑1 (MQ=255)
                                                                                            gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCa                                                   <  2:1298459/149‑1 (MQ=255)
                                                                                            gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCa                                                   <  1:1525165/149‑1 (MQ=255)
                                                                                             cATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCAc                                                  <  2:2695508/149‑1 (MQ=255)
                                                                                                     cTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTc                                          <  1:833843/149‑1 (MQ=255)
                                                                                                      tCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcg                                         >  1:101092/1‑149 (MQ=255)
                                                                                                        acaGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcgcg                                       >  2:1898756/1‑149 (MQ=255)
                                                                                                           gaaagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATc                                    >  1:1256791/1‑149 (MQ=255)
                                                                                                           gaaagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATc                                    >  2:4001530/1‑149 (MQ=255)
                                                                                                             aagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCg                                  >  1:1412736/1‑149 (MQ=255)
                                                                                                              agaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGc                                 <  1:1324517/149‑1 (MQ=255)
                                                                                                              agaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGc                                 >  1:3251156/1‑149 (MQ=255)
                                                                                                               gaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCt                                <  2:3174588/149‑1 (MQ=255)
                                                                                                                 aaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                              >  2:2428994/1‑149 (MQ=255)
                                                                                                                 aaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                              <  1:2621500/149‑1 (MQ=255)
                                                                                                                 aaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                              <  1:3812463/149‑1 (MQ=255)
                                                                                                                  aTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                             <  2:2453769/149‑1 (MQ=255)
                                                                                                                  aTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                             <  2:384009/149‑1 (MQ=255)
                                                                                                                    ccAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctc                           <  2:612514/149‑1 (MQ=255)
                                                                                                                     cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                          <  2:2303419/149‑1 (MQ=255)
                                                                                                                     cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                          <  1:2135938/149‑1 (MQ=255)
                                                                                                                     cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                          <  2:361656/149‑1 (MQ=255)
                                                                                                                     cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                          <  1:3500934/149‑1 (MQ=255)
                                                                                                                       gcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcctc                        >  1:2267081/1‑149 (MQ=255)
                                                                                                                       gcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcctc                        >  1:2302/1‑149 (MQ=255)
                                                                                                                        cagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCctct                       <  2:2041402/149‑1 (MQ=255)
                                                                                                                         agTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCGACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCtctc                      >  2:350494/1‑149 (MQ=255)
                                                                                                                         agTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCtctc                      >  1:4023815/1‑149 (MQ=255)
                                                                                                                          gTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCa                     >  1:479639/1‑149 (MQ=255)
                                                                                                                           ttCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAg                    >  1:3175475/1‑149 (MQ=255)
                                                                                                                              aaTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGa                 <  1:3272009/149‑1 (MQ=255)
                                                                                                                                 cAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGc              <  1:767625/149‑1 (MQ=255)
                                                                                                                                  aGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGca             >  2:3017891/1‑149 (MQ=255)
                                                                                                                                   gCTACAGCGCACCATAACCTTTATTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCTCTGGCTCCTCTCAGTGAAGcac            <  1:2968239/149‑1 (MQ=255)
                                                                                                                                   gCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac            >  1:674070/1‑149 (MQ=255)
                                                                                                                                   gCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac            >  2:1730149/1‑149 (MQ=255)
                                                                                                                                     tACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACaa          >  2:3708810/1‑149 (MQ=255)
                                                                                                                                         gcgcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCt      >  2:1390042/1‑149 (MQ=255)
                                                                                                                                          cgcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCtt     <  1:273397/149‑1 (MQ=255)
                                                                                                                                           gcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTa    <  1:3997973/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACT  >  CP009273/3455721‑3456010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑