Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,455,930 C→A 100% I76I (ATC→ATA)  gspJ → putative general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,455,9300CA100.0% 750.2 / ‑6.7 232I76I (ATC→ATA) gspJputative general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (114/117);  minor base T (0/1);  total (114/118)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

TTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATGGCA  >  CP009273/3455784‑3456075
                                                                                                                                                  |                                                                                                                                                 
tttATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAaataat                                                                                                                                                 <  1:3203949/149‑1 (MQ=255)
 ttATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATg                                                                                                                                                >  1:3310615/1‑149 (MQ=255)
  tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATgg                                                                                                                                               <  2:2790146/149‑1 (MQ=255)
  tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATgg                                                                                                                                               <  2:2697239/149‑1 (MQ=255)
  tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATgg                                                                                                                                               <  2:797396/149‑1 (MQ=255)
   aTGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgt                                                                                                                                              >  1:1002904/1‑149 (MQ=255)
   aTGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgt                                                                                                                                              <  2:1570673/149‑1 (MQ=255)
    tGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGgtg                                                                                                                                             <  1:1255451/149‑1 (MQ=255)
            tCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTga                                                                                                                                     <  1:63128/149‑1 (MQ=255)
             cGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaa                                                                                                                                    <  1:1179511/149‑1 (MQ=255)
             cGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaa                                                                                                                                    <  1:2468848/149‑1 (MQ=255)
              gCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaag                                                                                                                                   >  2:840250/1‑149 (MQ=255)
                aaGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaagaa                                                                                                                                 >  2:2804637/1‑149 (MQ=255)
                 aGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGaagaag                                                                                                                                >  1:180495/1‑149 (MQ=255)
                  gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGc                                                                                                                               >  2:1597294/1‑149 (MQ=255)
                    ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTa                                                                                                                             >  1:3272736/1‑149 (MQ=255)
                    ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTa                                                                                                                             <  2:1676395/149‑1 (MQ=255)
                      ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtt                                                                                                                           <  2:152944/149‑1 (MQ=255)
                        gAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtttt                                                                                                                         >  2:3041651/1‑149 (MQ=255)
                         aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAttttt                                                                                                                        >  1:1263204/1‑149 (MQ=255)
                         aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAttttt                                                                                                                        <  1:3106089/149‑1 (MQ=255)
                         aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAttttt                                                                                                                        >  1:1477058/1‑149 (MQ=255)
                           ttGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAc                                                                                                                      >  2:4074674/1‑149 (MQ=255)
                           ttGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAc                                                                                                                      >  2:4074643/1‑149 (MQ=255)
                            tGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAcc                                                                                                                     <  2:3111884/149‑1 (MQ=255)
                             gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCa                                                                                                                    <  2:2343351/149‑1 (MQ=255)
                             gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCa                                                                                                                    <  2:1298459/149‑1 (MQ=255)
                              cATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCAc                                                                                                                   <  2:2694849/149‑1 (MQ=255)
                              cATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCAc                                                                                                                   <  1:229418/149‑1 (MQ=255)
                                      cTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTc                                                                                                           <  1:833843/149‑1 (MQ=255)
                                      cTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTc                                                                                                           <  1:833558/149‑1 (MQ=255)
                                       tCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcg                                                                                                          >  1:101092/1‑149 (MQ=255)
                                        cacaGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcgc                                                                                                         >  2:1099765/1‑149 (MQ=255)
                                           agaaagaaATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGAt                                                                                                      <  1:3645744/149‑1 (MQ=255)
                                            gaaagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATACTGGTGTTGGGTGACGAAGCTATTTTTACCACGCACAGTCGCGATc                                                                                                     >  2:4001523/1‑149 (MQ=255)
                                             aaagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATcc                                                                                                    >  2:740382/1‑149 (MQ=255)
                                              aagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCg                                                                                                   >  1:1412736/1‑149 (MQ=255)
                                               agaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGc                                                                                                  <  2:2288685/149‑1 (MQ=255)
                                               agaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGc                                                                                                  <  2:2421343/149‑1 (MQ=255)
                                                gaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCt                                                                                                 <  2:3174697/149‑1 (MQ=255)
                                                  aaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                                                                                               <  1:2622983/149‑1 (MQ=255)
                                                  aaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                                                                                               <  1:3812554/149‑1 (MQ=255)
                                                  aaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                                                                                               <  1:3812324/149‑1 (MQ=255)
                                                   aTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                                                                                              <  2:384009/149‑1 (MQ=255)
                                                   aTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                                                                                              <  2:2453769/149‑1 (MQ=255)
                                                     ccAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctc                                                                                            <  2:612099/149‑1 (MQ=255)
                                                      cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                                                                                           <  2:361656/149‑1 (MQ=255)
                                                      cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                                                                                           <  1:3500934/149‑1 (MQ=255)
                                                      cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcc                                                                                           <  1:2135938/149‑1 (MQ=255)
                                                        gcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcctc                                                                                         >  1:2267081/1‑149 (MQ=255)
                                                         cagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCctct                                                                                        <  2:2041402/149‑1 (MQ=255)
                                                          agTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCGACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCtctc                                                                                       >  2:350494/1‑149 (MQ=255)
                                                           gTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCa                                                                                      <  2:3396881/149‑1 (MQ=255)
                                                           gTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCa                                                                                      >  2:863468/1‑149 (MQ=255)
                                                           gTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCa                                                                                      >  1:479639/1‑149 (MQ=255)
                                                               aaTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGa                                                                                  <  1:1882307/149‑1 (MQ=255)
                                                                aTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGaa                                                                                 >  1:633022/1‑149 (MQ=255)
                                                                aTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGaa                                                                                 >  2:2474014/1‑149 (MQ=255)
                                                                 tCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAg                                                                                >  2:2067811/1‑149 (MQ=255)
                                                                 tCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAg                                                                                >  1:2233837/1‑149 (MQ=255)
                                                                  cAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCATGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGc                                                                               <  1:767491/149‑1 (MQ=255)
                                                                   aGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGca                                                                              >  2:3017891/1‑149 (MQ=255)
                                                                    gCTACAGCGCACCATAACCTTTATTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCTCTGGCTCCTCTCAGTGAAGcac                                                                             <  1:2968239/149‑1 (MQ=255)
                                                                    gCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac                                                                             >  2:1730149/1‑149 (MQ=255)
                                                                      tACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACaa                                                                           >  2:3708810/1‑149 (MQ=255)
                                                                        cAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAAc                                                                         <  1:1401543/149‑1 (MQ=255)
                                                                           cgcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCtt                                                                      <  1:2454628/149‑1 (MQ=255)
                                                                           cgcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCtt                                                                      <  1:273397/149‑1 (MQ=255)
                                                                            gcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTa                                                                     <  1:3997973/149‑1 (MQ=255)
                                                                              accataaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACt                                                                   >  1:3262160/1‑149 (MQ=255)
                                                                                     ccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGtt                                                            <  1:3449759/149‑1 (MQ=255)
                                                                                              aTAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATTATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCTCTGGTAt                                                   <  1:414750/149‑1 (MQ=255)
                                                                                               tAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATc                                                  >  2:2828923/1‑149 (MQ=255)
                                                                                                 aCGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTg                                                >  2:62993/1‑149 (MQ=255)
                                                                                                 aCGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTg                                                <  2:1859215/149‑1 (MQ=255)
                                                                                                    aTCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGa                                             >  2:3436680/1‑149 (MQ=255)
                                                                                                      ctcCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGaaa                                           >  2:3761277/1‑149 (MQ=255)
                                                                                                       tcCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAAt                                          >  2:3006080/1‑149 (MQ=255)
                                                                                                       tcCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAAt                                          >  1:1087452/1‑149 (MQ=255)
                                                                                                        ccTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATc                                         >  1:2200073/1‑149 (MQ=255)
                                                                                                         cTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCa                                        <  1:234237/149‑1 (MQ=255)
                                                                                                           gCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATa                                      >  1:2063188/1‑149 (MQ=255)
                                                                                                              aCTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGc                                   >  1:1540032/1‑149 (MQ=255)
                                                                                                                tGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGctc                                 <  1:2563501/149‑1 (MQ=255)
                                                                                                                 ggTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGctct                                <  2:1220501/149‑1 (MQ=255)
                                                                                                                    cGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTAcc                             >  2:3149860/1‑149 (MQ=255)
                                                                                                                      cccGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGt                           <  2:480717/149‑1 (MQ=255)
                                                                                                                          gaggaATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTg                       >  2:680584/1‑149 (MQ=255)
                                                                                                                              aaTCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCg                   <  1:786856/149‑1 (MQ=255)
                                                                                                                               aTCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGt                  >  1:4114246/1‑149 (MQ=255)
                                                                                                                                       aCGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCtgt          >  1:3709313/1‑149 (MQ=255)
                                                                                                                                       aCGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCtgt          >  1:3707960/1‑149 (MQ=255)
                                                                                                                                       aCGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCtgt          >  2:1977121/1‑149 (MQ=255)
                                                                                                                                        cGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCtgtg         >  2:3806959/1‑149 (MQ=255)
                                                                                                                                            cAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATg     >  2:3324638/1‑149 (MQ=255)
                                                                                                                                             aaaaTAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATgg    >  1:697483/1‑149 (MQ=255)
                                                                                                                                             aaaaTAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATgg    >  2:2655693/1‑149 (MQ=255)
                                                                                                                                             aaaaTAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATgg    >  1:3533460/1‑149 (MQ=255)
                                                                                                                                              aaaTAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATGGc   >  1:2409814/1‑149 (MQ=255)
                                                                                                                                               aataatGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATGGCa  >  2:3317084/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACTGTTCACTGGTATCTGCGAAATCATACGCTCTACCGTGCTGTTCGTACTTCTGTGGATGGCA  >  CP009273/3455784‑3456075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑