Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,470,272 C→A 100% V169V (GTG→GTT)  fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,470,2720CA98.2% 880.3 / 3.1 276V169V (GTG→GTT) fkpAFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (5/0);  new base A (126/145);  total (131/145)
Fisher's exact test for biased strand distribution p-value = 2.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

ACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCG  >  CP009273/3470126‑3470417
                                                                                                                                                  |                                                                                                                                                 
aCCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAAc                                                                                                                                                 <  2:1190340/149‑1 (MQ=255)
   aGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTac                                                                                                                                              >  2:2941337/1‑149 (MQ=255)
     tttGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaa                                                                                                                                            >  2:1731738/1‑149 (MQ=255)
       tGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCAGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaaca                                                                                                                                          <  1:1378744/149‑1 (MQ=255)
        gATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAg                                                                                                                                         <  2:3034759/149‑1 (MQ=255)
        gATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAg                                                                                                                                         <  2:3873753/149‑1 (MQ=255)
          tCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTa                                                                                                                                       >  2:164137/1‑149 (MQ=255)
          tCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTa                                                                                                                                       >  2:164893/1‑149 (MQ=255)
            ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATc                                                                                                                                     <  2:1546604/149‑1 (MQ=255)
            ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATc                                                                                                                                     >  1:4075420/1‑149 (MQ=255)
              aCCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGc                                                                                                                                   <  2:84029/149‑1 (MQ=255)
              aCCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGc                                                                                                                                   <  1:1064729/149‑1 (MQ=255)
                  ccTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTc                                                                                                                               >  2:1058606/1‑149 (MQ=255)
                  ccTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTc                                                                                                                               <  1:115422/149‑1 (MQ=255)
                   ctgtCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCt                                                                                                                              <  2:2540683/146‑1 (MQ=255)
                   cTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCt                                                                                                                              <  1:1028018/149‑1 (MQ=255)
                   cTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCt                                                                                                                              >  1:2471299/1‑149 (MQ=255)
                    tttCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCtt                                                                                                                             <  2:2023092/149‑1 (MQ=255)
                      tCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTc                                                                                                                           <  2:61924/149‑1 (MQ=255)
                        ttGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTCGTTAACTACAACAGTATCGGGGTCTTTCgg                                                                                                                         >  2:1114626/1‑149 (MQ=255)
                        ttGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCgg                                                                                                                         <  2:2800432/149‑1 (MQ=255)
                         tGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGt                                                                                                                        <  2:2128641/149‑1 (MQ=255)
                          gATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTg                                                                                                                       >  1:2205116/1‑149 (MQ=255)
                           aTGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGc                                                                                                                      >  2:1592982/1‑149 (MQ=255)
                            tGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCt                                                                                                                     >  2:799505/1‑149 (MQ=255)
                              ttcttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCGTTCGGTGCTTc                                                                                                                   >  2:2995415/1‑149 (MQ=255)
                                cttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGc                                                                                                                 <  2:3771968/149‑1 (MQ=255)
                                       ccTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAcc                                                                                                          <  1:3952814/149‑1 (MQ=255)
                                       ccTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAcc                                                                                                          <  2:2243916/149‑1 (MQ=255)
                                         ttCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCgg                                                                                                        >  1:288431/1‑149 (MQ=255)
                                           cTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCt                                                                                                      <  1:834729/149‑1 (MQ=255)
                                                 aaCCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTctact                                                                                                <  2:4062646/149‑1 (MQ=255)
                                                      gggATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTATTACCTg                                                                                           >  2:3212564/1‑149 (MQ=255)
                                                       ggATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGa                                                                                          >  2:2754612/1‑149 (MQ=255)
                                                          tAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATaa                                                                                       >  1:2068375/1‑149 (MQ=255)
                                                           aaCACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTGTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATaaa                                                                                      >  1:1407683/1‑149 (MQ=255)
                                                           aaCACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATaaa                                                                                      <  1:2379679/149‑1 (MQ=255)
                                                            acacCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAac                                                                                     >  1:3246757/1‑149 (MQ=255)
                                                                 gTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagac                                                                                >  2:2869240/1‑149 (MQ=255)
                                                                  tCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagacc                                                                               >  1:2732552/1‑149 (MQ=255)
                                                                  tCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagacc                                                                               <  1:138886/149‑1 (MQ=255)
                                                                    cAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagaccag                                                                             <  1:2315053/149‑1 (MQ=255)
                                                                    cAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagaccag                                                                             <  1:1513235/149‑1 (MQ=255)
                                                                      gACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGtt                                                                           >  2:1475369/1‑149 (MQ=255)
                                                                         ggAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGaa                                                                        >  2:1011925/1‑149 (MQ=255)
                                                                            aagaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAagag                                                                     <  1:308574/149‑1 (MQ=255)
                                                                            aagaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAagag                                                                     <  1:164893/149‑1 (MQ=255)
                                                                              gaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGt                                                                   <  1:3981333/149‑1 (MQ=255)
                                                                               atagCGGTTCACCACGTGTGTAAGAGTTGTCGAACTCTTGACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGtt                                                                  <  1:2164907/147‑1 (MQ=255)
                                                                               aaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGtt                                                                  <  1:651011/149‑1 (MQ=255)
                                                                               aaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGtt                                                                  <  1:3775222/149‑1 (MQ=255)
                                                                                aagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGttt                                                                 <  1:158209/149‑1 (MQ=255)
                                                                                aagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGttt                                                                 >  2:288960/1‑149 (MQ=255)
                                                                                 agCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGtttt                                                                >  2:1332015/1‑149 (MQ=255)
                                                                                     gTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTcaca                                                            <  2:3525119/149‑1 (MQ=255)
                                                                                       tCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACAcc                                                          <  1:3129766/149‑1 (MQ=255)
                                                                                        caccacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCt                                                         <  1:3577257/149‑1 (MQ=255)
                                                                                         accacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCtt                                                        <  2:3766670/149‑1 (MQ=255)
                                                                                         accacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCtt                                                        <  2:290381/149‑1 (MQ=255)
                                                                                            acGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtct                                                     <  1:1131650/149‑1 (MQ=255)
                                                                                               ggTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCtt                                                  >  1:97046/1‑149 (MQ=255)
                                                                                               ggTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCtt                                                  <  2:4107888/149‑1 (MQ=255)
                                                                                                gtgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCttt                                                 <  2:707757/149‑1 (MQ=255)
                                                                                                 tgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTTTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAACGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTGACACCTTTCTATTTg                                                >  2:1586464/1‑149 (MQ=255)
                                                                                                 tgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTg                                                <  2:1829842/149‑1 (MQ=255)
                                                                                                 tgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATAGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTg                                                >  2:1747386/1‑149 (MQ=255)
                                                                                                     agagTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaa                                            >  2:3318965/1‑149 (MQ=255)
                                                                                                     agagTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaa                                            >  1:2716695/1‑149 (MQ=255)
                                                                                                     agagTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaa                                            >  1:194199/1‑149 (MQ=255)
                                                                                                      gagTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaaa                                           <  2:2732538/149‑1 (MQ=255)
                                                                                                       agTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAAt                                          <  2:3132623/149‑1 (MQ=255)
                                                                                                        gTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTATTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAAtt                                         <  1:3212564/149‑1 (MQ=255)
                                                                                                        gTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAAtt                                         <  2:495258/149‑1 (MQ=255)
                                                                                                           gTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCGTTCTCTTTGGCCAATTtct                                      >  1:3909218/1‑149 (MQ=255)
                                                                                                           gTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTtct                                      <  1:914476/149‑1 (MQ=255)
                                                                                                              gAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcgc                                   >  2:2712146/1‑149 (MQ=255)
                                                                                                               aaCTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcgcg                                  >  2:3254212/1‑149 (MQ=255)
                                                                                                                aCTCTTTACCGTCGATCATCGTACCTTTGTAGTTAAATACAACAGTATAGCTTTCTTTAGGTGCTTCGCCTTTACCGGCGTCTACTACCTGATAAACCAGACAAGTTGAAGAGGTTTTAACACCATTATGTTTTGCAAATTTCTCGCgg                                 >  1:2497240/1‑149 (MQ=255)
                                                                                                                aCTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGGTTCTAATACCTGATAAACCAGACCAGTTGAATAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCgg                                 >  1:719563/1‑149 (MQ=255)
                                                                                                                aCTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCGTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCgg                                 >  1:3160386/1‑149 (MQ=255)
                                                                                                                 ctctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTGGCGGt                                >  1:3431798/1‑149 (MQ=255)
                                                                                                                  tctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTa                               <  1:633761/149‑1 (MQ=255)
                                                                                                                   ctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGTTAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTAc                              >  2:3556714/1‑149 (MQ=255)
                                                                                                                   ctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTAc                              <  1:3005191/149‑1 (MQ=255)
                                                                                                                   ctTTACCGTAGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTAc                              <  2:898646/149‑1 (MQ=255)
                                                                                                                      tACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTActct                           <  2:3827413/149‑1 (MQ=255)
                                                                                                                          gTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTAc                       <  1:1752300/149‑1 (MQ=255)
                                                                                                                             gATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCtt                    >  1:2423100/1‑149 (MQ=255)
                                                                                                                              aTCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCttt                   >  1:3600446/1‑149 (MQ=255)
                                                                                                                              aTCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCttt                   <  1:1813171/149‑1 (MQ=255)
                                                                                                                               tCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCtttt                  >  1:225152/1‑149 (MQ=255)
                                                                                                                                 aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGc                <  1:3049829/149‑1 (MQ=255)
                                                                                                                                  gCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCt               <  1:798101/149‑1 (MQ=255)
                                                                                                                                     tACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTAGCTTTTGCTTCg            >  2:1212533/1‑149 (MQ=255)
                                                                                                                                     tACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCg            >  2:3380510/1‑149 (MQ=255)
                                                                                                                                      aCCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGt           <  2:1923871/149‑1 (MQ=255)
                                                                                                                                         tttGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTAt        >  2:563910/1‑149 (MQ=255)
                                                                                                                                         tttGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTAt        <  1:2798388/149‑1 (MQ=255)
                                                                                                                                         tttGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTAt        <  1:3338209/149‑1 (MQ=255)
                                                                                                                                          ttGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATc       <  1:2583598/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCG  >  CP009273/3470126‑3470417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑