Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,471,705 C→T 100% G51D (GGT→GAT)  slyD ← FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,471,7050CT98.7% 745.9 / 0.0 226G51D (GGT→GAT) slyDFKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (108/114);  total (111/115)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

TTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTA  >  CP009273/3471559‑3471849
                                                                                                                                                  |                                                                                                                                                
 ttCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCtt                                                                                                                                               <  1:225171/149‑1 (MQ=255)
  tCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTc                                                                                                                                              <  1:3507871/149‑1 (MQ=255)
  tCAGCCAGGAAACACATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTc                                                                                                                                              <  1:2352771/149‑1 (MQ=255)
    aGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCa                                                                                                                                            <  2:2472001/149‑1 (MQ=255)
       cAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAgcg                                                                                                                                         >  2:843485/1‑149 (MQ=255)
        aGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAgcgc                                                                                                                                        >  1:1021564/1‑149 (MQ=255)
        aGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAgcgc                                                                                                                                        >  1:2873756/1‑149 (MQ=255)
         ggAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGcc                                                                                                                                       >  1:470172/1‑149 (MQ=255)
          gAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCg                                                                                                                                      >  1:1424223/1‑149 (MQ=255)
            aaCGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGtt                                                                                                                                    <  2:2125615/149‑1 (MQ=255)
             aCGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGttt                                                                                                                                   <  1:1110165/149‑1 (MQ=255)
                cATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCa                                                                                                                                <  2:137355/149‑1 (MQ=255)
                 aTACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAg                                                                                                                               <  1:987365/149‑1 (MQ=255)
                 aTACCTACCTGCAGGTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAg                                                                                                                               <  2:3519565/149‑1 (MQ=255)
                  tacctaccTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAgg                                                                                                                              >  2:2561426/1‑149 (MQ=255)
                  tacctaccTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAgg                                                                                                                              >  1:265648/1‑149 (MQ=255)
                   acctacctGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGc                                                                                                                             >  1:2860697/1‑149 (MQ=255)
                    cctacctGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGcc                                                                                                                            <  1:3807625/149‑1 (MQ=255)
                       acctGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCaga                                                                                                                         >  1:2287815/1‑149 (MQ=255)
                         ctGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAgaga                                                                                                                       <  1:2184046/149‑1 (MQ=255)
                          tGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGAt                                                                                                                      >  2:3768757/1‑149 (MQ=255)
                           gCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATc                                                                                                                     <  2:1939350/149‑1 (MQ=255)
                               ttCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAggg                                                                                                                 >  2:1111049/1‑149 (MQ=255)
                                 catcaACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGGCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGaa                                                                                                               <  1:1452449/149‑1 (MQ=255)
                                     aaCGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGt                                                                                                           >  2:3094065/1‑149 (MQ=255)
                                     aaCGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGt                                                                                                           >  1:3985045/1‑149 (MQ=255)
                                     aaCGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGt                                                                                                           <  1:1560624/149‑1 (MQ=255)
                                       cGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGa                                                                                                         >  2:1311755/1‑149 (MQ=255)
                                         cccATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGAcc                                                                                                       >  2:626368/1‑149 (MQ=255)
                                             tAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGc                                                                                                   <  2:687716/149‑1 (MQ=255)
                                               aaTACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAg                                                                                                 <  1:240107/149‑1 (MQ=255)
                                                aTACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAgg                                                                                                >  2:1461559/1‑149 (MQ=255)
                                                  aCGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTa                                                                                              <  1:1182124/149‑1 (MQ=255)
                                                     tCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTc                                                                                           <  1:3006969/149‑1 (MQ=255)
                                                       tttAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCa                                                                                         >  2:643473/1‑149 (MQ=255)
                                                       tttAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCa                                                                                         >  2:643389/1‑149 (MQ=255)
                                                       tttAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCa                                                                                         <  2:1977884/149‑1 (MQ=255)
                                                        ttAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAg                                                                                        <  1:716685/149‑1 (MQ=255)
                                                         tAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAgc                                                                                       <  2:1424223/149‑1 (MQ=255)
                                                             aaCACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGACCCGTGACCATGCAGGTAGTCCAgcggcg                                                                                   >  2:2450568/1‑149 (MQ=255)
                                                              acacGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGgcgc                                                                                  >  2:3210830/1‑149 (MQ=255)
                                                                acGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCAc                                                                                <  2:2051390/149‑1 (MQ=255)
                                                                         cAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGgag                                                                       <  1:2499443/149‑1 (MQ=255)
                                                                         cAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGgag                                                                       <  2:1128901/149‑1 (MQ=255)
                                                                          aGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGGCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGgaga                                                                      <  1:1825468/149‑1 (MQ=255)
                                                                           ggTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGAc                                                                     >  1:3581259/1‑149 (MQ=255)
                                                                           ggTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGAc                                                                     >  2:830103/1‑149 (MQ=255)
                                                                            gTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACt                                                                    >  1:519009/1‑149 (MQ=255)
                                                                               ttCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGAGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACtcat                                                                 <  2:524560/149‑1 (MQ=255)
                                                                                  gtcgtACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATcaa                                                              <  2:77396/149‑1 (MQ=255)
                                                                                    cgtACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATcaacc                                                            <  2:2808393/149‑1 (MQ=255)
                                                                                    cgtACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATcaacc                                                            <  1:3747858/149‑1 (MQ=255)
                                                                                     gtACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATcaacca                                                           >  1:488179/1‑149 (MQ=255)
                                                                                     gtACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTACAGGCCCGAGATCAGGGACCCGTGACCCTGCAGGTAGTCCAGCGGCGCACTCACCCGAGACTCATcaacca                                                           >  1:2553795/1‑149 (MQ=255)
                                                                                       aCTGACCGTAAGCGTCGCTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATcaaccaac                                                         >  2:1334810/1‑149 (MQ=255)
                                                                                         tGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAacac                                                       >  1:801777/1‑149 (MQ=255)
                                                                                          gACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAacaca                                                      <  2:3849483/149‑1 (MQ=255)
                                                                                           aCCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAacacac                                                     <  2:3557651/149‑1 (MQ=255)
                                                                                              gTAAGCGTCGTTCGCGCCAACAGCGACATCAAAATTGTCGCCAACTTCATGAACTTCCATCGCCGATTCCAGGCCAGAGATCAGGGAAACCTGACCATGCAGGTAGTACAGCGGCGCACTCACAGGAGACTCAAAAACCAACACACCga                                                  >  1:2754486/1‑148 (MQ=255)
                                                                                               tAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCCGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCCCTCACCGGAGACTCATCAACCAACACACCGtc                                                 >  1:901330/1‑149 (MQ=255)
                                                                                               tAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtc                                                 <  2:1239270/149‑1 (MQ=255)
                                                                                               tAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtc                                                 >  2:2883230/1‑149 (MQ=255)
                                                                                               tAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtc                                                 <  2:150531/149‑1 (MQ=255)
                                                                                                 aGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtctt                                               <  1:1419258/149‑1 (MQ=255)
                                                                                                  gCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGtcttc                                              <  2:3038740/149‑1 (MQ=255)
                                                                                                     tcgtTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgt                                           <  2:3296938/149‑1 (MQ=255)
                                                                                                      cgtTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgta                                          >  1:3268989/1‑149 (MQ=255)
                                                                                                       gtTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgtac                                         >  2:2531948/1‑149 (MQ=255)
                                                                                                        ttCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgtacg                                        >  2:1558591/1‑149 (MQ=255)
                                                                                                         tagcgcCAACAGCGACATCAATATTGTCGCCAACTTCATTATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGTGAACCGTGACCATGCTGGTAGTCCAGAGGCGGACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgttcgt                                       <  2:2138632/147‑1 (MQ=255)
                                                                                                           gcgcCAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTgtacgtac                                     <  2:389553/149‑1 (MQ=255)
                                                                                                               cAACAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGa                                 <  2:2932437/149‑1 (MQ=255)
                                                                                                                 aCAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATa                               <  2:3363646/149‑1 (MQ=255)
                                                                                                                 aCAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATa                               >  2:546808/1‑149 (MQ=255)
                                                                                                                 aCAGCGACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGGACGTACCTGATa                               >  1:387996/1‑149 (MQ=255)
                                                                                                                     cGACATCAACTTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGcc                           <  2:3992807/149‑1 (MQ=255)
                                                                                                                      gACATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCa                          <  1:626390/149‑1 (MQ=255)
                                                                                                                       aCATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAg                         >  2:51193/1‑149 (MQ=255)
                                                                                                                        cATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAgg                        >  2:1567797/1‑149 (MQ=255)
                                                                                                                        cATCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAgg                        >  2:2297539/1‑149 (MQ=255)
                                                                                                                         aTCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGc                       <  1:1866847/149‑1 (MQ=255)
                                                                                                                          tCAAATTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCt                      <  1:134132/149‑1 (MQ=255)
                                                                                                                              aTTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGacc                  >  2:3699581/1‑149 (MQ=255)
                                                                                                                              aTTTGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGacc                  >  2:1175985/1‑149 (MQ=255)
                                                                                                                               tttGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGacca                 <  2:157114/149‑1 (MQ=255)
                                                                                                                               tttGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGacca                 >  1:2014292/1‑149 (MQ=255)
                                                                                                                                 tggcGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGaccacc               <  2:2040580/146‑1 (MQ=255)
                                                                                                                                 tGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGaccacc               <  2:1833169/149‑1 (MQ=255)
                                                                                                                                 tGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGaccacc               <  2:16862/149‑1 (MQ=255)
                                                                                                                                 tGTCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGaccacc               <  1:3094065/149‑1 (MQ=255)
                                                                                                                                   tCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAg             <  2:2040408/149‑1 (MQ=255)
                                                                                                                                   tCGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAg             >  2:1451561/1‑149 (MQ=255)
                                                                                                                                    cGCCAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAgg            <  1:78481/149‑1 (MQ=255)
                                                                                                                                       cAACTTCATGATCTTCCAGCGCCGTTTCCAGGCCACATAACAGGGAAACGTTACCATTCAGGTAGTCCAGCGGCGCCCTCACCGGAGACTCATCAAACAACACACCGTCTTCTGTACGTACCAGATAGGCCCCGCTGACCACCAGGTCt         >  1:1179672/1‑149 (MQ=255)
                                                                                                                                        aaCTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCCGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCtt        >  2:913833/1‑149 (MQ=255)
                                                                                                                                         aCTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCttt       <  1:1558591/149‑1 (MQ=255)
                                                                                                                                         aCTTCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCttt       <  2:2290795/149‑1 (MQ=255)
                                                                                                                                           ttCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTg     >  1:4059138/1‑149 (MQ=255)
                                                                                                                                           ttCATGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTg     >  1:4059447/1‑149 (MQ=255)
                                                                                                                                              aTGATCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTa  >  2:2258836/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAACACGTTGCACCAGGTTTTCGTCGTACTGACCGTAAGCGTCGTTCGCGCCAACAGCGACATCAAATTTGTCGCCAACTTCATGACCTTCCAGCGCCGTTTCCAGGCCAGAGATCAGGGAACCGTGACCATGCAGGTAGTCCAGCGGCGCACTCACCGGAGACTCATCAACCAACACACCGTCTTCTGTACGTACCTGATAGGCCAGGCTGACCACCAGGTCTTTTGCTA  >  CP009273/3471559‑3471849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑