Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,479,769 A→T 100% E97D (GAA→GAT)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,479,7690AT99.7% 930.5 / ‑4.7 279E97D (GAA→GAT) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (135/143);  total (135/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 281 total aligned reads displayed.

GCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCAC  >  CP009273/3479623‑3479914
                                                                                                                                                  |                                                                                                                                                 
gCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGatat                                                                                                                                                 >  2:3761557/1‑149 (MQ=255)
        gATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACa                                                                                                                                         >  1:1168672/1‑149 (MQ=255)
         aTCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTCCGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaa                                                                                                                                        >  2:3114944/1‑149 (MQ=255)
         aTCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaa                                                                                                                                        >  1:332525/1‑149 (MQ=255)
             aaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaaaa                                                                                                                                    >  1:1034553/1‑149 (MQ=255)
              agaagaagaGGGTAAAGAAATGATCATCTACTATCTGAATCAGGGTGATTTTATTGGCGATCTGGGCCTGTTTGATGAGGGCCAGGATCGTAGCGCTTGGGTTCGTGAGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAAt                                                                                                                                   <  1:3050475/149‑1 (MQ=255)
                 cGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTc                                                                                                                                <  1:803517/149‑1 (MQ=255)
                     gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCa                                                                                                                            >  1:175028/1‑149 (MQ=255)
                     gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCa                                                                                                                            >  1:198285/1‑149 (MQ=255)
                        ggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGGACAAAAAATTTCGCCAAtt                                                                                                                         >  2:213821/1‑149 (MQ=255)
                          tAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGa                                                                                                                       >  1:2649037/1‑149 (MQ=255)
                          tAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGa                                                                                                                       <  2:4011451/149‑1 (MQ=255)
                          tAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGa                                                                                                                       <  2:1049128/149‑1 (MQ=255)
                              gAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCa                                                                                                                   >  2:4099941/1‑149 (MQ=255)
                                actGATCCTCTCCTATCTGCATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAgg                                                                                                                 <  1:4003394/147‑1 (MQ=255)
                                  tGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa                                                                                                               >  2:2553355/1‑149 (MQ=255)
                                    aTCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                                                                             >  1:1605021/1‑149 (MQ=255)
                                     tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                                                                            >  1:3119095/1‑149 (MQ=255)
                                      ccTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                                                                                                           <  2:1513269/149‑1 (MQ=255)
                                       ctctCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                                                                          <  1:126186/149‑1 (MQ=255)
                                       ctctCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                                                                          <  1:2553355/149‑1 (MQ=255)
                                        tctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                                                                         >  2:3456819/1‑149 (MQ=255)
                                        tctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                                                                         <  2:4102832/149‑1 (MQ=255)
                                         ctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                                                                                                        <  2:130375/149‑1 (MQ=255)
                                          tcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGa                                                                                                       <  2:3036949/149‑1 (MQ=255)
                                           ccTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGAGTCAGGTAAACCCGGAc                                                                                                      >  2:1000733/1‑149 (MQ=255)
                                            cTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                                                                     <  1:1016675/149‑1 (MQ=255)
                                             tATCTGACTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                    <  1:2526780/149‑1 (MQ=255)
                                             tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                    <  1:2931949/149‑1 (MQ=255)
                                             tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                    <  2:3572700/149‑1 (MQ=255)
                                             tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                    <  1:1874894/149‑1 (MQ=255)
                                              aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                                                   >  2:111695/1‑149 (MQ=255)
                                               tCTGAATCATGTTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACACGGACATTc                                                                                                  <  2:2850348/149‑1 (MQ=255)
                                               tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                  <  2:3213634/149‑1 (MQ=255)
                                               tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                  <  1:628801/149‑1 (MQ=255)
                                               tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                  <  2:2359128/149‑1 (MQ=255)
                                               tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                  <  2:281150/149‑1 (MQ=255)
                                               tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                  >  1:3220647/1‑149 (MQ=255)
                                                 tGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                                                                                >  1:3390670/1‑149 (MQ=255)
                                                 tGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                                                                                >  2:1109356/1‑149 (MQ=255)
                                                  gAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTTATTCAGGTAAACCCGGACATTCTGa                                                                                               <  1:272429/149‑1 (MQ=255)
                                                  gAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                                                                               <  2:2225828/149‑1 (MQ=255)
                                                  gAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                                                                               >  2:265947/1‑149 (MQ=255)
                                                   aaTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                                                                              <  2:208991/149‑1 (MQ=255)
                                                    aTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                                                                             >  2:1278097/1‑149 (MQ=255)
                                                     tCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                                            <  1:1505564/149‑1 (MQ=255)
                                                     tCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                                            >  1:2838566/1‑149 (MQ=255)
                                                       aGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                                                                          >  1:2031332/1‑149 (MQ=255)
                                                          gTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTg                                                                                       >  2:610147/1‑149 (MQ=255)
                                                          gTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTg                                                                                       >  1:498828/1‑149 (MQ=255)
                                                          gTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTg                                                                                       >  2:2930799/1‑149 (MQ=255)
                                                           tGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                                                                      <  2:1611624/149‑1 (MQ=255)
                                                           tGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                                                                      <  2:3656447/149‑1 (MQ=255)
                                                                   ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                                                                              <  1:357566/149‑1 (MQ=255)
                                                                       cGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                                                                          <  1:2354833/149‑1 (MQ=255)
                                                                       cGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                                                                          <  1:1340709/149‑1 (MQ=255)
                                                                             gggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                                                                    >  1:391782/1‑149 (MQ=255)
                                                                                ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                                                                 <  2:1545411/149‑1 (MQ=255)
                                                                                   gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc                                                              >  1:2058617/1‑149 (MQ=255)
                                                                                   gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc                                                              >  1:4093797/1‑149 (MQ=255)
                                                                                         agagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa                                                        >  1:2414433/1‑149 (MQ=255)
                                                                                              gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa                                                   <  2:259868/149‑1 (MQ=255)
                                                                                              gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa                                                   >  2:3938856/1‑149 (MQ=255)
                                                                                              gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa                                                   >  1:2101408/1‑149 (MQ=255)
                                                                                              gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa                                                   <  1:597869/149‑1 (MQ=255)
                                                                                                cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCaga                                                 <  2:1846246/149‑1 (MQ=255)
                                                                                                 aGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCagag                                                >  2:58158/1‑149 (MQ=255)
                                                                                                 aGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCagag                                                <  2:3645767/149‑1 (MQ=255)
                                                                                                  ggAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAgaga                                               <  2:2777919/149‑1 (MQ=255)
                                                                                                    aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGaaa                                             >  2:1930528/1‑149 (MQ=255)
                                                                                                    aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGaaa                                             <  2:1407/149‑1 (MQ=255)
                                                                                                        tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTgg                                         <  1:2163612/149‑1 (MQ=255)
                                                                                                        tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTgg                                         <  2:805664/149‑1 (MQ=255)
                                                                                                        tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTgg                                         <  1:3431634/149‑1 (MQ=255)
                                                                                                        tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTgg                                         >  1:1675173/1‑149 (MQ=255)
                                                                                                          gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGAGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGc                                       >  1:2325824/1‑149 (MQ=255)
                                                                                                            gcATGGGGACGTGCGAAAACCGCCTGTGAAGTGGCTGATAGTTCGTACAAAAAAATTCGCCAATTGATTCAGGTAAACCCGGACATTCTGCTGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCaa                                     <  1:1419443/149‑1 (MQ=255)
                                                                                                              aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAAcc                                   >  1:3639720/1‑149 (MQ=255)
                                                                                                               tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                  >  2:442644/1‑149 (MQ=255)
                                                                                                                       tGCGAAAACCGCCTGTGAATTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGAATCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGGCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGCCGTTcc                          >  1:3148487/1‑149 (MQ=255)
                                                                                                                       tGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTcc                          >  2:343496/1‑149 (MQ=255)
                                                                                                                        gCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCt                         <  1:1868030/149‑1 (MQ=255)
                                                                                                                           aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGa                      <  1:3761557/149‑1 (MQ=255)
                                                                                                                            aaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTCCACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                     <  2:2415445/149‑1 (MQ=255)
                                                                                                                            aaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                     >  2:180777/1‑149 (MQ=255)
                                                                                                                            aaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                     >  1:1754912/1‑149 (MQ=255)
                                                                                                                            aaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                     >  2:2552754/1‑149 (MQ=255)
                                                                                                                              aCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGACAGTGGGCAACCTGGCGTTCCTCGACGt                   >  1:2248692/1‑149 (MQ=255)
                                                                                                                              aCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGt                   <  1:1213806/149‑1 (MQ=255)
                                                                                                                                  ccTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACg               <  2:273626/149‑1 (MQ=255)
                                                                                                                                  ccTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACg               >  2:1991099/1‑149 (MQ=255)
                                                                                                                                   cTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACgg              <  2:1523024/149‑1 (MQ=255)
                                                                                                                                     gtgAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGc            >  1:3258543/1‑149 (MQ=255)
                                                                                                                                      tgAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGcc           >  1:1949344/1‑149 (MQ=255)
                                                                                                                                       gAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCg          >  1:370948/1‑149 (MQ=255)
                                                                                                                                          gTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAt       >  2:3818730/1‑149 (MQ=255)
                                                                                                                                           tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCTGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt      <  2:175115/149‑1 (MQ=255)
                                                                                                                                           tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt      <  2:198285/149‑1 (MQ=255)
                                                                                                                                           tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt      <  1:2695099/149‑1 (MQ=255)
                                                                                                                                               tGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac  >  2:1658389/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCAC  >  CP009273/3479623‑3479914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑