BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,486,738	A→T	50.7%	Q271L (CAA→CTA)	tsgA →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,486,738	0	A	T	50.7%	12.1 / inf	237	Q271L (CAA→CTA)	tsgA	putative transporter
Reads supporting (aligned to +/- strand): ref base A (63/54); new base T (66/54); total (129/108)
Fisher's exact test for biased strand distribution p-value = 8.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

GTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGT  >  CP009273/3486592‑3486880

gTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTGTTTGATTTGCAAc                                                                                                                                              >  1:2758223/1‑149 (MQ=255)

gTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAAc                                                                                                                                              >  1:2262304/1‑149 (MQ=255)

     gTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCAtt                                                                                                                                         >  2:428435/1‑149 (MQ=255)

      ttttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTc                                                                                                                                        <  2:4026781/149‑1 (MQ=255)

      ttttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTc                                                                                                                                        <  2:3317937/149‑1 (MQ=255)

      ttttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTc                                                                                                                                        <  1:291209/149‑1 (MQ=255)

      ttttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTc                                                                                                                                        >  1:2344444/1‑149 (MQ=255)

       tttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCt                                                                                                                                       <  2:693363/149‑1 (MQ=255)

       tttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCt                                                                                                                                       >  2:1889985/1‑149 (MQ=255)

             tcCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGt                                                                                                                                 <  1:2147424/149‑1 (MQ=255)

               cTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTAc                                                                                                                               >  1:3333480/1‑149 (MQ=255)

                tGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTAct                                                                                                                              <  1:1099173/149‑1 (MQ=255)

                  ggTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTActgg                                                                                                                            <  1:3803860/149‑1 (MQ=255)

                      ccTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTActggctgg                                                                                                                        <  1:2495473/149‑1 (MQ=255)

                      ccTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTActggctgg                                                                                                                        >  1:2879387/1‑149 (MQ=255)

                           gTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTgg                                                                                                                   >  1:3593920/1‑149 (MQ=255)

                            tATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGc                                                                                                                  >  2:2372903/1‑149 (MQ=255)

                             aTGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCt                                                                                                                 >  1:3106341/1‑149 (MQ=255)

                              tGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTg                                                                                                                >  1:3458167/1‑149 (MQ=255)

                                 cAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGa                                                                                                             >  1:3143535/1‑149 (MQ=255)

                                 cAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGa                                                                                                             <  1:3789328/149‑1 (MQ=255)

                                    aGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTc                                                                                                          <  1:388509/149‑1 (MQ=255)

                                     ggCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCt                                                                                                         >  2:1574766/1‑149 (MQ=255)

                                      gCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTg                                                                                                        >  1:2350482/1‑149 (MQ=255)

                                        cTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGAt                                                                                                      >  2:2711497/1‑149 (MQ=255)

                                          gggCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGt                                                                                                    >  2:2727165/1‑149 (MQ=255)

                                           ggCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTa                                                                                                   <  2:2103591/149‑1 (MQ=255)

                                              aTGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGt                                                                                                >  1:622948/1‑149 (MQ=255)

                                               tGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTc                                                                                               >  1:2053234/1‑149 (MQ=255)

                                                 aGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCtt                                                                                             <  1:52870/149‑1 (MQ=255)

                                                    cTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTaa                                                                                          >  2:638935/1‑149 (MQ=255)

                                                    cTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTaa                                                                                          >  2:639143/1‑149 (MQ=255)

                                                    cTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTaa                                                                                          <  2:3423415/149‑1 (MQ=255)

                                                    cTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTaa                                                                                          <  1:1143884/149‑1 (MQ=255)

                                                     tGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAac                                                                                         >  2:2531934/1‑149 (MQ=255)

                                                      gAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAaca                                                                                        <  1:1877659/149‑1 (MQ=255)

                                                      gAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAaca                                                                                        <  1:268508/149‑1 (MQ=255)

                                                       aaCGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAacac                                                                                       <  2:2412305/149‑1 (MQ=255)

                                                         cgacgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCg                                                                                     <  2:1373426/149‑1 (MQ=255)

                                                          gacgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCgg                                                                                    >  1:12994/1‑149 (MQ=255)

                                                          gacgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCgg                                                                                    >  1:2399653/1‑149 (MQ=255)

                                                           acgCGGGCACGCTGGTGAGTAACTTCTGGATGTCTTAGATGGTCGGCATGTGGGCGTTCAGCTTTATTCGTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGATGCGATTCTGATGTACGTCTTTAACACCGGa                                                                                   <  2:3983713/149‑1 (MQ=255)

                                                            cgcgGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGaa                                                                                  <  2:1501032/149‑1 (MQ=255)

                                                               gggCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAacac                                                                               <  2:1964885/149‑1 (MQ=255)

                                                               gggCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAacac                                                                               <  2:526171/149‑1 (MQ=255)

                                                               gggCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAacac                                                                               <  1:3222517/149‑1 (MQ=255)

                                                                   aCGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGGTTCTTTGATTTGCAACGCATTCTGAACGTACTGTCTGGTCTGGCGGCGATTCTGATGTACGTCTTTAACACCGGAACACCatc                                                                           >  1:74724/1‑147 (MQ=255)

                                                                        ggTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACata                                                                      <  1:864115/149‑1 (MQ=255)

                                                                        ggTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACata                                                                      >  2:736075/1‑149 (MQ=255)

                                                                          tGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATg                                                                    <  2:1784083/149‑1 (MQ=255)

                                                                          tGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTAAGTCTTTAACACCGGAACACCAGCACATATg                                                                    >  1:138185/1‑149 (MQ=255)

                                                                            aGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGc                                                                  >  2:285170/1‑149 (MQ=255)

                                                                             gTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCg                                                                 >  2:1199807/1‑149 (MQ=255)

                                                                             gTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCg                                                                 >  2:1315235/1‑149 (MQ=255)

                                                                               aaCTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTTACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTg                                                               >  2:2081788/1‑149 (MQ=255)

                                                                               aaCTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTg                                                               >  1:2558192/1‑149 (MQ=255)

                                                                                 cTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATCTGGCGTGGt                                                             >  2:103088/1‑149 (MQ=255)

                                                                                 cTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGt                                                             >  1:269452/1‑149 (MQ=255)

                                                                                  ttCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTc                                                            <  1:1627786/149‑1 (MQ=255)

                                                                                    cTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCaa                                                          <  2:3856801/149‑1 (MQ=255)

                                                                                     tGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAAt                                                         <  1:2381458/149‑1 (MQ=255)

                                                                                         tGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATtctc                                                     >  2:373260/1‑149 (MQ=255)

                                                                                           tCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGc                                                   >  2:537721/1‑149 (MQ=255)

                                                                                           tCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGc                                                   >  2:299382/1‑149 (MQ=255)

                                                                                              tACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGctct                                                >  2:3302394/1‑149 (MQ=255)

                                                                                                cATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTgg                                              >  2:185542/1‑149 (MQ=255)

                                                                                                 aTGGTCGGCATGTGGGCGTTCAGCTTTATTATTCGCTTCTTTGATTTGCAACGCAATCTTACCCTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTggg                                             >  1:2320547/1‑149 (MQ=255)

                                                                                                    gTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGctt                                          <  2:2215235/149‑1 (MQ=255)

                                                                                                     tCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTTCTACGCAATCTGACCGTACAGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttc                                         >  1:3873846/1‑149 (MQ=255)

                                                                                                       ggCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                       >  1:3693082/1‑149 (MQ=255)

                                                                                                       ggCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                       >  1:1247926/1‑149 (MQ=255)

                                                                                                       ggCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                       <  1:3419679/149‑1 (MQ=255)

                                                                                                       ggCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                       <  1:3260293/149‑1 (MQ=255)

                                                                                                              gggCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAgc                                <  1:175214/149‑1 (MQ=255)

                                                                                                               ggCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTAAGTCTTTAAAACCGGAACACCAGCACAAATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAgcg                               >  1:3233606/1‑149 (MQ=255)

                                                                                                                gCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAgcgc                              >  2:780907/1‑149 (MQ=255)

                                                                                                                   ttCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGAt                           >  1:4027499/1‑149 (MQ=255)

                                                                                                                     cAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCt                         <  1:428435/149‑1 (MQ=255)

                                                                                                                      aGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCta                        <  1:302615/149‑1 (MQ=255)

                                                                                                                      aGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTATTTAACACAGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCta                        >  2:3159642/1‑149 (MQ=255)

                                                                                                                       gCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtat                       <  1:1186816/149‑1 (MQ=255)

                                                                                                                         tttATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATac                     >  2:2771827/1‑149 (MQ=255)

                                                                                                                         tttATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATac                     >  2:3203612/1‑149 (MQ=255)

                                                                                                                             ttcttcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATaccacc                 >  1:2306633/1‑149 (MQ=255)

                                                                                                                              tcttcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGACCACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATAccacca                <  1:3323360/149‑1 (MQ=255)

                                                                                                                               cttcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACcat               <  1:2726430/149‑1 (MQ=255)

                                                                                                                                 tcGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACcatca             >  2:3832930/1‑149 (MQ=255)

                                                                                                                                 tcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACcatca             <  2:3030493/149‑1 (MQ=255)

                                                                                                                                   gCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCatcatc           <  1:1967230/149‑1 (MQ=255)

                                                                                                                                   gCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATAACACCatcatc           >  2:2514725/1‑149 (MQ=255)

                                                                                                                                    cttcttTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCAtcatca          >  2:3944998/1‑149 (MQ=255)

                                                                                                                                      tcttTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTTGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAcc        >  1:3608373/1‑149 (MQ=255)

                                                                                                                                      tcttTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAcc        <  2:4024327/149‑1 (MQ=255)

                                                                                                                                       cttTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAccc       >  2:2459359/1‑149 (MQ=255)

                                                                                                                                         ttGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTg     <  2:4101703/149‑1 (MQ=255)

                                                                                                                                         ttGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTg     >  1:3791854/1‑149 (MQ=255)

                                                                                                                                         ttGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTg     <  1:4063131/149‑1 (MQ=255)

                                                                                                                                         ttGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTg     <  2:3334852/149‑1 (MQ=255)

                                                                                                                                           gATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCAccaacgt   <  2:3143516/149‑6 (MQ=255)

                                                                                                                                           gATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTggg   >  1:2570703/1‑149 (MQ=255)

GTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGT  >  CP009273/3486592‑3486880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`