Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,501,571 C→A 100% G127C (GGC→TGC)  php ← phosphotriesterase homology protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,501,5710CA99.3% 911.5 / ‑3.3 275G127C (GGC→TGC) phpphosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (138/135);  total (140/135)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 275 total aligned reads displayed.

GTGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCC  >  CP009273/3501425‑3501716
                                                                                                                                                  |                                                                                                                                                 
gTGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGa                                                                                                                                                 <  2:3108396/149‑1 (MQ=255)
gTGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGa                                                                                                                                                 >  1:68321/1‑149 (MQ=255)
 tGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGAt                                                                                                                                                >  1:3634361/1‑149 (MQ=255)
   ggCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGAtct                                                                                                                                              <  2:349495/149‑1 (MQ=255)
          aGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCgat                                                                                                                                       >  2:2095240/1‑149 (MQ=255)
              aCGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATc                                                                                                                                   >  1:1003417/1‑149 (MQ=255)
              aCGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTGTCCTTCGCTGGTGCAGATCTCCGCGATGATc                                                                                                                                   <  1:2221927/149‑1 (MQ=255)
               cGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATcc                                                                                                                                  <  2:2451560/149‑1 (MQ=255)
                gCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATccc                                                                                                                                 >  1:2438851/1‑149 (MQ=255)
                     ttGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCtt                                                                                                                            <  2:29463/149‑1 (MQ=255)
                       gCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTc                                                                                                                          <  2:731229/149‑1 (MQ=255)
                        cTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCa                                                                                                                         <  2:460281/149‑1 (MQ=255)
                            aGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTc                                                                                                                     <  1:1128388/149‑1 (MQ=255)
                             gCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTcc                                                                                                                    <  1:1556170/149‑1 (MQ=255)
                             gCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTcc                                                                                                                    >  1:781841/1‑149 (MQ=255)
                                ccATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTg                                                                                                                 >  1:2004502/1‑149 (MQ=255)
                                 cATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                                >  1:1208770/1‑149 (MQ=255)
                                 cATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                                >  1:2976874/1‑149 (MQ=255)
                                  aTCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGcc                                                                                                               >  2:3798075/1‑149 (MQ=255)
                                   tCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCa                                                                                                              >  1:2129581/1‑149 (MQ=255)
                                   tCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCa                                                                                                              <  2:2671129/149‑1 (MQ=255)
                                   tCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCa                                                                                                              >  1:816237/1‑149 (MQ=255)
                                    cGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCAt                                                                                                             <  2:2110609/149‑1 (MQ=255)
                                         tGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATa                                                                                                        <  2:2302927/149‑1 (MQ=255)
                                             cgacgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCt                                                                                                    <  2:3172818/149‑1 (MQ=255)
                                             cgacgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCt                                                                                                    <  2:3634186/149‑1 (MQ=255)
                                             cgacgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCt                                                                                                    >  1:3214769/1‑149 (MQ=255)
                                               acgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGt                                                                                                  <  2:8834/149‑1 (MQ=255)
                                                  tATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCa                                                                                               >  1:1633095/1‑149 (MQ=255)
                                                    tGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAt                                                                                             <  2:269386/149‑1 (MQ=255)
                                                     gCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAtt                                                                                            <  2:2499400/149‑1 (MQ=255)
                                                     gCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAtt                                                                                            <  2:2400930/149‑1 (MQ=255)
                                                     gCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAtt                                                                                            <  1:565036/149‑1 (MQ=255)
                                                        tGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCa                                                                                         <  2:3670117/149‑1 (MQ=255)
                                                         ggAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCAt                                                                                        >  1:2561407/1‑149 (MQ=255)
                                                            gaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGa                                                                                     >  2:1367745/1‑149 (MQ=255)
                                                                 ggCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtc                                                                                >  2:2430956/1‑149 (MQ=255)
                                                                 ggCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtc                                                                                >  1:1138311/1‑149 (MQ=255)
                                                                  gCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtct                                                                               >  1:3335669/1‑149 (MQ=255)
                                                                  gCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtct                                                                               <  1:460959/149‑1 (MQ=255)
                                                                   cGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtctc                                                                              >  2:2699464/1‑149 (MQ=255)
                                                                    gTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTcc                                                                             <  1:829370/149‑1 (MQ=255)
                                                                        ggTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTgcg                                                                         >  2:3791989/1‑149 (MQ=255)
                                                                          tCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGcc                                                                       >  1:101193/1‑149 (MQ=255)
                                                                           cTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCa                                                                      >  1:2932536/1‑149 (MQ=255)
                                                                             ggTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTCCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGt                                                                    <  1:844789/149‑1 (MQ=255)
                                                                                tATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTcc                                                                 <  1:3926928/149‑1 (MQ=255)
                                                                                              gcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                   <  1:3057103/149‑1 (MQ=255)
                                                                                               cgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGTTACCCTGTTCAAGTTCATCGACCATCTCCTGCGCCCGTTCCTGCACGCTGCggggc                                                  >  2:3636796/1‑147 (MQ=255)
                                                                                               cgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGGTCCTGCACGCTGCGGGTc                                                  <  2:259352/149‑1 (MQ=255)
                                                                                                ggCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCg                                                 >  2:3245078/1‑149 (MQ=255)
                                                                                                ggCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCg                                                 >  1:893865/1‑149 (MQ=255)
                                                                                                ggCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCg                                                 >  1:3106639/1‑149 (MQ=255)
                                                                                                   aataaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCca                                              <  1:2254866/149‑1 (MQ=255)
                                                                                                     taaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGTCCATCTCCTGCGCGAGTTCCTGCACGCTGCGGGTCGCcaca                                            >  2:1369040/1‑149 (MQ=255)
                                                                                                      aaataCCTTCTCTTCCCGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCACTTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACAt                                           <  1:3492507/149‑1 (MQ=255)
                                                                                                      aaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACAt                                           <  2:253150/149‑1 (MQ=255)
                                                                                                      aaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACAt                                           <  2:1159322/149‑1 (MQ=255)
                                                                                                         taCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGtt                                        >  2:1950282/1‑149 (MQ=255)
                                                                                                          aCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTc                                       <  2:3060508/149‑1 (MQ=255)
                                                                                                          aCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTc                                       >  2:3310742/1‑149 (MQ=255)
                                                                                                          aCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTc                                       <  2:1003417/149‑1 (MQ=255)
                                                                                                           ccTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTcc                                      <  1:2034552/149‑1 (MQ=255)
                                                                                                           ccTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTcc                                      <  1:1981993/149‑1 (MQ=255)
                                                                                                              tctcTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCggg                                   >  1:2737108/1‑149 (MQ=255)
                                                                                                              tctcTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCggg                                   <  1:1771274/149‑1 (MQ=255)
                                                                                                                tctTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaa                                 >  2:1060624/1‑149 (MQ=255)
                                                                                                                tctTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaa                                 <  1:3955807/149‑1 (MQ=255)
                                                                                                                 ctTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaaa                                <  2:3175816/149‑1 (MQ=255)
                                                                                                                  ttCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaaaa                               >  2:2179557/1‑149 (MQ=255)
                                                                                                                   tCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaaaaa                              <  2:3882155/149‑1 (MQ=255)
                                                                                                                   tCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaaaaa                              <  1:1060624/149‑1 (MQ=255)
                                                                                                                    ccAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAc                             <  2:687149/149‑1 (MQ=255)
                                                                                                                      aGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcgc                           <  2:2244519/149‑1 (MQ=255)
                                                                                                                      aGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcgc                           >  2:753992/1‑149 (MQ=255)
                                                                                                                      aGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcgc                           >  2:5404/1‑149 (MQ=255)
                                                                                                                       gcggcgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAACAAcgcg                          >  2:484842/1‑149 (MQ=255)
                                                                                                                       gcggcgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcgcg                          >  2:482582/1‑149 (MQ=255)
                                                                                                                           cgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCt                      <  2:300365/149‑1 (MQ=255)
                                                                                                                            gTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTg                     <  2:2976874/149‑1 (MQ=255)
                                                                                                                             tAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTgg                    >  2:2220988/1‑149 (MQ=255)
                                                                                                                                tCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTACAATTTCATCGACCATCTCCTGCGCCGGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGtaa                 <  1:358595/149‑1 (MQ=255)
                                                                                                                                  tttCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGACAAACGCGTCCTGGtaata               >  2:2410991/1‑149 (MQ=255)
                                                                                                                                  tttCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGtcata               >  2:3163774/1‑149 (MQ=255)
                                                                                                                                   ttCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGtaataa              >  2:1593852/1‑149 (MQ=255)
                                                                                                                                   ttCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGtaataa              >  1:507324/1‑149 (MQ=255)
                                                                                                                                    tCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAAc             <  1:381231/149‑1 (MQ=255)
                                                                                                                                    tCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAAc             >  2:1997902/1‑149 (MQ=255)
                                                                                                                                     ccTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAAcc            <  2:3983364/149‑1 (MQ=255)
                                                                                                                                      cTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCg           >  2:1734797/1‑149 (MQ=255)
                                                                                                                                        tCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGt         >  2:3399119/1‑149 (MQ=255)
                                                                                                                                          gCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTAc       >  1:2231706/1‑149 (MQ=255)
                                                                                                                                          gCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTAc       >  1:1849667/1‑149 (MQ=255)
                                                                                                                                            tGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGCAACACGCGTCCTGGTAATAACCGGTACAg     >  2:2821901/1‑149 (MQ=255)
                                                                                                                                            tGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAg     >  1:1440672/1‑149 (MQ=255)
                                                                                                                                             ggTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAgg    >  1:3588975/1‑149 (MQ=255)
                                                                                                                                              gTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGc   <  2:1049267/149‑1 (MQ=255)
                                                                                                                                               tGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCCTCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGcc  <  2:942041/149‑1 (MQ=255)
                                                                                                                                               tGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGcc  <  2:2386714/149‑1 (MQ=255)
                                                                                                                                               tGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGcc  <  2:1802959/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCC  >  CP009273/3501425‑3501716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑