Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 31,710 C→A 100% I298I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927331,7100CA98.7% 664.7 / ‑0.5 220I298I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (131/86);  total (133/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 222 total aligned reads displayed.

TCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCC  >  CP009273/31565‑31854
                                                                                                                                                 |                                                                                                                                                
 cgctCCAGCCCAAACGCTGTCCGACAAAGAATTTCAAATCATGCGTAACGCCTCGATGGCGGTGCTGAGTGAAATCGGCTTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaa                                                                                                                                               <  1:1132043/148‑1 (MQ=255)
  gcgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaaa                                                                                                                                              <  2:375948/148‑1 (MQ=255)
    gcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATg                                                                                                                                            <  1:649119/148‑1 (MQ=255)
    gcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATg                                                                                                                                            <  1:649442/148‑1 (MQ=255)
      cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGaa                                                                                                                                          <  2:2955567/148‑1 (MQ=255)
      cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGaa                                                                                                                                          >  1:180894/1‑148 (MQ=255)
      cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTGTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGaa                                                                                                                                          <  2:1652173/148‑1 (MQ=255)
        gCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAcc                                                                                                                                        >  1:2210865/1‑148 (MQ=255)
          ccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCa                                                                                                                                      <  2:237075/148‑1 (MQ=255)
           cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAc                                                                                                                                     >  2:169524/1‑148 (MQ=255)
            aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGGTCCAACGTTCAGTTTGCGGTGAAACCGAAAAACGGTCGTCTTATTGTTATAGAAATGAACCCAcg                                                                                                                                    >  1:3328864/1‑148 (MQ=255)
             aaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgc                                                                                                                                   >  2:2838806/1‑148 (MQ=255)
             aaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgc                                                                                                                                   >  1:2372021/1‑148 (MQ=255)
             aaCGCTGACAGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgc                                                                                                                                   >  2:1558088/1‑148 (MQ=255)
              aCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgcg                                                                                                                                  >  1:1031801/1‑148 (MQ=255)
                   gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTcc                                                                                                                             >  2:1757663/1‑148 (MQ=255)
                    aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTccc                                                                                                                            >  1:443739/1‑148 (MQ=255)
                     ccGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCg                                                                                                                           >  1:1107134/1‑148 (MQ=255)
                       gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGtt                                                                                                                         <  2:180797/148‑1 (MQ=255)
                        aCAAAGAATCTCAACTCCTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttc                                                                                                                        <  2:2127965/148‑1 (MQ=255)
                        aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttc                                                                                                                        <  1:2336462/148‑1 (MQ=255)
                        aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttc                                                                                                                        <  2:3005663/148‑1 (MQ=255)
                        aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCATCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttc                                                                                                                        >  2:1948754/1‑148 (MQ=255)
                         cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                       <  2:252694/148‑1 (MQ=255)
                          aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttctt                                                                                                                      <  2:3361208/148‑1 (MQ=255)
                           aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                     >  2:2741348/1‑148 (MQ=255)
                            aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                    >  1:2489613/1‑148 (MQ=255)
                            aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                    >  2:2697430/1‑148 (MQ=255)
                            aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                    >  1:658531/1‑148 (MQ=255)
                            aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                    >  1:2139947/1‑148 (MQ=255)
                             gAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCgg                                                                                                                   >  2:260081/1‑148 (MQ=255)
                              aaTATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgc                                                                                                                  >  2:597861/1‑148 (MQ=255)
                              aaTATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgc                                                                                                                  >  1:1729165/1‑148 (MQ=255)
                               atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcg                                                                                                                 <  2:2386233/148‑1 (MQ=255)
                                tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcgc                                                                                                                >  2:745322/1‑148 (MQ=255)
                                 atCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                               >  1:2572570/1‑148 (MQ=255)
                                 atCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                               >  2:1345139/1‑148 (MQ=255)
                                   cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTgg                                                                                                             >  1:2980924/1‑148 (MQ=255)
                                   cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTgg                                                                                                             >  1:1563851/1‑148 (MQ=255)
                                       tCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTc                                                                                                         >  1:1195792/1‑148 (MQ=255)
                                        cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                                        >  1:3247551/1‑148 (MQ=255)
                                        cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                                        >  1:576658/1‑148 (MQ=255)
                                          tGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaa                                                                                                      >  2:3536908/1‑148 (MQ=255)
                                               accGCCTCGAGGGCGGTGCGGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCTGTTTGCGGTCAACCCGAAAAACGGTCGTCTGTTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGa                                                                                                 <  2:1238433/146‑1 (MQ=255)
                                               aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                 >  2:3153742/1‑148 (MQ=255)
                                                aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAc                                                                                                >  2:818280/1‑148 (MQ=255)
                                                 cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                               >  1:2943354/1‑148 (MQ=255)
                                                 cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                               >  1:2214235/1‑148 (MQ=255)
                                                 cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                               >  1:1671341/1‑148 (MQ=255)
                                                   ccTCGATGGGGGTGGTGCGTTAAATCGGCTTTGAAACCGGTGTTTCAAAGTTTCTTTTTGGGGTTAACCCGAAAAAGGGTGGTCTTATTTTTATATAAATGAACCCACGGGTGTCCGGTTCTTCGGCGCTGGCGTCTAAAGCGACCgg                                                                                             <  2:1063613/148‑1 (MQ=255)
                                                      cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                          >  2:106614/1‑148 (MQ=255)
                                                       gATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTc                                                                                         >  1:1718017/1‑148 (MQ=255)
                                                        aggggggtggtGCGTTAAATCGGCGTTGAACCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCTAAAAACGGTGGTCTGATTTTTATACAAATCAACCCACGGGTGGCCCGTTCTTCGGCGCTGGGGTCGAAAGCGACCGGTTTcc                                                                                        <  2:2775386/143‑1 (MQ=255)
                                                         tGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                                                       >  1:730125/1‑148 (MQ=255)
                                                         tGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                                                       >  1:798719/1‑148 (MQ=255)
                                                         tGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                                                       >  2:3421229/1‑148 (MQ=255)
                                                           gCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGa                                                                                     >  1:3136632/1‑148 (MQ=255)
                                                                gctgcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCt                                                                                >  2:1592638/1‑148 (MQ=255)
                                                                gctgcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCt                                                                                >  1:86163/1‑148 (MQ=255)
                                                                 ctggGTGAAATCGGCGTTGAACCCGGTGGTTCCAACGTTCCGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGGGGCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTa                                                                               <  2:2040367/148‑1 (MQ=255)
                                                                      tGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                          >  2:873347/1‑148 (MQ=255)
                                                                       taaaTCGGCGTTGAAACGGGTGGTTCCAACGTTCAGTTTGTGGTGACCCCGAAAAACGGTCGTCTTTTTGTTATAGAAATGAACCCACGCGTGGCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                                         <  2:2942691/147‑1 (MQ=255)
                                                                       gAATTCGGCGTTTAACCCGGTGGTTCCAACGTTCATTTTGCGGTGAACCCCAAAACGGGTCGTCTGTTGTTTATAGAAATCAACCCCCGCGTGTCCGGTTCTTCGGCGCTGGCTTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                                         <  2:1612275/148‑1 (MQ=255)
                                                                          aTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcgg                                                                      >  1:3008520/1‑148 (MQ=255)
                                                                               cGTTGAATCGGGTGGTACCAACTTTCAGTTGGCGGTGAACCCTAAAATGGGTCGGCTTTTTTTTATAGAAATCACCCCACGCGTGTCCCGTTCTTGGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                 <  2:629744/148‑1 (MQ=255)
                                                                                gTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTTTTATAGAAATGAACCCACGCGTGTCCCGTACTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAc                                                                <  2:1499083/148‑1 (MQ=255)
                                                                                  tGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                                              >  1:2757516/1‑148 (MQ=255)
                                                                                   gAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTgg                                                             >  2:852437/1‑148 (MQ=255)
                                                                                       ccGGTGGTTCCAACGTTCAGTTTGCGGTCAACCCTAAAAACGGTCGTCGGATTGTTATAGAAATGACCCCACGCGTGTCCCGTTCTGCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGt                                                         <  2:2174376/148‑1 (MQ=255)
                                                                                        cGGTGGTTCCAACGTTCTGTTTGCGGTGAACCCGAAAAACGGTGGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTg                                                        <  2:2898476/148‑1 (MQ=255)
                                                                                           tggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGt                                                     >  1:2018765/1‑148 (MQ=255)
                                                                                             gtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTa                                                   >  1:2646265/1‑148 (MQ=255)
                                                                                               tCCAACGTTCTGTGGGCGGCCAACCCGAAAAAGGGTCGTCTGATTTTTATAGAAATCACCCCCCGCGGGCCCCGTTCTTCGGCGGTGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTaca                                                 <  1:138549/148‑1 (MQ=255)
                                                                                                ccAACGTCCAGTGTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCGGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTacac                                                <  2:2083245/148‑1 (MQ=255)
                                                                                                 cAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAcc                                               >  1:2871530/1‑148 (MQ=255)
                                                                                                 cAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAcc                                               >  1:2871546/1‑148 (MQ=255)
                                                                                                 cAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAcc                                               >  1:3479596/1‑148 (MQ=255)
                                                                                                      ttCAGTTTGCGGTCAACCCTAAAAAGGGTCGTCTTTTTTTTATACAAATCAACCCCCGGGTGTCCGGTTCTTGGGCGGTGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcga                                          <  1:3088438/148‑1 (MQ=255)
                                                                                                       tCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgac                                         >  1:2968341/1‑148 (MQ=255)
                                                                                                        cAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacg                                        >  2:2413872/1‑148 (MQ=255)
                                                                                                           tttGCGGTCAACCCTAAAAACGGTCGTCTTTTTGTTATACAAATCACCCCCCGGGGGTCCGGTTGTGCGGCGGTGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                     <  1:745856/148‑1 (MQ=255)
                                                                                                           tgtGCGGTCACCCCTAAAACGGTTGGTGTGATTTTTATACAAATCACCCCCCGGGGGCCCCGTCGTGCGGGGGGGCCGCCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                     <  1:3418000/146‑1 (MQ=255)
                                                                                                             tGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtg                                   >  2:2319473/1‑148 (MQ=255)
                                                                                                              ggggTGAACCCGAAAAAGGGTCGTCTTATTTTTATAGAAATCAACCCACGCGGGGCCCGTTCTTCGGCGGTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtga                                  <  2:1892862/146‑1 (MQ=255)
                                                                                                                  tGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                              >  2:3493031/1‑148 (MQ=255)
                                                                                                                    aaCCCTAAAAAGGGTGGTATGATTGTTATATAAATGAACCCACGGGGGGCCCGTTCTTCGGCGGTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACg                            <  2:1869319/148‑1 (MQ=255)
                                                                                                                    aaCCCTAAAAAGGGTCGTCTGATTTTTTTATAATTGACCCCCCGGGTGTCCCGTTCTCCGGGGCGGGCTTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACg                            <  2:3283315/148‑1 (MQ=255)
                                                                                                                           aaaaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCAc                     >  1:2286211/1‑148 (MQ=255)
                                                                                                                              aCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTgg                  <  2:2214235/148‑1 (MQ=255)
                                                                                                                                  tcgtcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGa              >  2:1612895/1‑148 (MQ=255)
                                                                                                                                      cttaTTGTTATACAAATGAACCCACGCGGGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa          <  2:1723710/145‑1 (MQ=255)
                                                                                                                                      cTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa          >  1:2683803/1‑148 (MQ=255)
                                                                                                                                      cTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa          >  1:99702/1‑148 (MQ=255)
                                                                                                                                       tGATTGTTATAGAAATGAACCCACGCGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTAc         <  2:3181447/148‑1 (MQ=255)
                                                                                                                                        gATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTAGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACt        <  1:2091188/148‑1 (MQ=255)
                                                                                                                                         tttGTTATAGAAATCACCCCACGCGTGTCCCGTTCTGCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTc       <  2:3039977/147‑1 (MQ=255)
                                                                                                                                         aTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTc       >  2:2063831/1‑148 (MQ=255)
                                                                                                                                          ttGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTcc      >  2:885883/1‑148 (MQ=255)
                                                                                                                                           tGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCg     >  1:1937642/1‑148 (MQ=255)
                                                                                                                                             ttATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc   >  2:959703/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCC  >  CP009273/31565‑31854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑