Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,258,871 G→T 100% L173I (CTC→ATC)  tdcB ← L‑threonine dehydratase, catabolic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,258,8710GT96.1% 533.9 / 12.1 178L173I (CTC→ATC) tdcBL‑threonine dehydratase, catabolic
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (80/91);  total (84/94)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 181 total aligned reads displayed.

TGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAC  >  CP009273/3258726‑3259015
                                                                                                                                                 |                                                                                                                                                
tGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGatat                                                                                                                                                <  1:3184813/148‑1 (MQ=255)
     agaagCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTcc                                                                                                                                           <  1:944073/148‑1 (MQ=255)
     agaagCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTcc                                                                                                                                           >  2:3046136/1‑148 (MQ=255)
     agaagCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTcc                                                                                                                                           >  2:3408608/1‑148 (MQ=255)
        agCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATa                                                                                                                                        <  1:3331211/148‑1 (MQ=255)
        agCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATa                                                                                                                                        <  1:3431387/148‑1 (MQ=255)
         gccgccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATaa                                                                                                                                       >  2:2383320/1‑148 (MQ=255)
          ccgccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAt                                                                                                                                      <  1:2841844/148‑1 (MQ=255)
          ccgccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAt                                                                                                                                      <  2:1623697/148‑1 (MQ=255)
          ccgccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAt                                                                                                                                      <  2:2747004/148‑1 (MQ=255)
            gccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAttt                                                                                                                                    <  1:796494/148‑1 (MQ=255)
              cATGCCGTGAACGTTTTCAGTCTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTcc                                                                                                                                  <  2:199243/148‑1 (MQ=255)
               aTGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCa                                                                                                                                 >  1:1421117/1‑148 (MQ=255)
               aTGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCa                                                                                                                                 >  1:2330967/1‑148 (MQ=255)
                  ccGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAc                                                                                                                              >  1:3288044/1‑148 (MQ=255)
                        aCGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCg                                                                                                                        >  1:1706172/1‑148 (MQ=255)
                          gTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGtt                                                                                                                      >  1:1401655/1‑148 (MQ=255)
                          gTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGtt                                                                                                                      <  1:3500918/148‑1 (MQ=255)
                          gTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGtt                                                                                                                      <  1:600653/148‑1 (MQ=255)
                          gTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGtt                                                                                                                      >  1:3168865/1‑148 (MQ=255)
                              tCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCt                                                                                                                  <  2:2541874/148‑1 (MQ=255)
                                 gACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGc                                                                                                               <  2:253139/148‑1 (MQ=255)
                                  aCTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGcc                                                                                                              >  2:1202820/1‑148 (MQ=255)
                                        cGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCANTTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAAt                                                                                                        <  1:1806646/148‑1 (MQ=255)
                                         gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATc                                                                                                       <  2:2843042/148‑1 (MQ=255)
                                         gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATc                                                                                                       <  2:42566/148‑1 (MQ=255)
                                         gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATc                                                                                                       <  2:1191908/148‑1 (MQ=255)
                                          ccAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCa                                                                                                      <  1:1602234/148‑1 (MQ=255)
                                           cAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCAc                                                                                                     <  1:1153373/148‑1 (MQ=255)
                                             aTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACtt                                                                                                   >  2:3139279/1‑148 (MQ=255)
                                              tAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACttt                                                                                                  >  2:2211693/1‑148 (MQ=255)
                                              tAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACttt                                                                                                  >  2:2211587/1‑148 (MQ=255)
                                               aaCACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTc                                                                                                 <  1:2693524/148‑1 (MQ=255)
                                               aaCACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTc                                                                                                 >  2:533604/1‑148 (MQ=255)
                                                  acGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGa                                                                                              <  1:1137468/148‑1 (MQ=255)
                                                   cGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGat                                                                                             <  2:2350172/148‑1 (MQ=255)
                                                    gAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatc                                                                                            <  2:3288044/148‑1 (MQ=255)
                                                    gAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatc                                                                                            >  2:888670/1‑148 (MQ=255)
                                                     aaTGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatca                                                                                           <  1:468665/148‑1 (MQ=255)
                                                     aaTGGTCGGATTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGGTATCTTCCATAATTTCCAGACCAATCGTGCCCTGTCCAGCACTCACTTTCgcaaca                                                                                           >  1:1820910/1‑143 (MQ=255)
                                                       tGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatcatc                                                                                         >  1:70677/1‑148 (MQ=255)
                                                        ggTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCg                                                                                        >  2:1955440/1‑148 (MQ=255)
                                                        ggTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCg                                                                                        >  1:2541303/1‑148 (MQ=255)
                                                          tCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa                                                                                      <  2:648306/148‑1 (MQ=255)
                                                          tCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa                                                                                      >  1:3487780/1‑148 (MQ=255)
                                                           cGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTaa                                                                                     >  1:2425814/1‑148 (MQ=255)
                                                           cGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTaa                                                                                     >  1:2232564/1‑148 (MQ=255)
                                                              gTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTGTCGGATCATCGTAAGGt                                                                                  <  2:2869341/148‑1 (MQ=255)
                                                                 aaTAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTAGGATCATCGTAAGGTggg                                                                               >  1:557614/1‑148 (MQ=255)
                                                                  aTAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGa                                                                              >  2:1670705/1‑148 (MQ=255)
                                                                  aTAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGa                                                                              <  1:2627381/148‑1 (MQ=255)
                                                                        ttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATaaaaa                                                                        >  1:208397/1‑148 (MQ=255)
                                                                        ttAATTGCCACCGCAATACCAGCAAGTAAACCGCCACCACCAATTGGCACAATAACGTTATCGACATCAGAGATATCTTCCATAATTTCCAGACCAATCGTTCCCGGGCCAGCAATCACTTTCGGATCAAATGAAGGTGGGATaaaat                                                                        >  1:2612028/1‑147 (MQ=255)
                                                                         tAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAAt                                                                       <  2:70688/148‑1 (MQ=255)
                                                                          aaTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATa                                                                      <  1:1954362/148‑1 (MQ=255)
                                                                            ttGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACg                                                                    >  1:130154/1‑148 (MQ=255)
                                                                              tccACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGc                                                                  >  2:1618434/2‑148 (MQ=255)
                                                                              gCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGc                                                                  <  2:1091651/148‑1 (MQ=255)
                                                                              gCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGc                                                                  >  2:3129451/1‑148 (MQ=255)
                                                                              gCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGc                                                                  <  1:3352303/148‑1 (MQ=255)
                                                                               ccACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGcc                                                                 <  2:3463586/148‑1 (MQ=255)
                                                                                 aCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtt                                                               >  1:1083492/1‑148 (MQ=255)
                                                                                 aCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtt                                                               <  2:1618939/148‑1 (MQ=255)
                                                                                     cAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAt                                                           >  1:689935/1‑148 (MQ=255)
                                                                                               aaTTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAAtt                                                 >  2:182007/1‑148 (MQ=255)
                                                                                                aTTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAAttt                                                >  2:3306098/1‑148 (MQ=255)
                                                                                                 ttAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTc                                               >  1:3045456/1‑148 (MQ=255)
                                                                                                     aCCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTc                                           >  2:1395686/1‑148 (MQ=255)
                                                                                                      ccGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCa                                          >  1:2757723/1‑148 (MQ=255)
                                                                                                       cGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCAc                                         <  1:3054407/148‑1 (MQ=255)
                                                                                                       cGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCAc                                         >  1:2818798/1‑148 (MQ=255)
                                                                                                         ccaccaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACtt                                       >  2:1943456/1‑148 (MQ=255)
                                                                                                             caccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGc                                   <  1:1572498/148‑1 (MQ=255)
                                                                                                              accaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCg                                  <  1:3390580/148‑1 (MQ=255)
                                                                                                                   ttGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgt                             >  2:2669353/1‑148 (MQ=255)
                                                                                                                   ttGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgt                             <  1:2916011/148‑1 (MQ=255)
                                                                                                                    tGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgtg                            <  1:539910/148‑1 (MQ=255)
                                                                                                                     ggCACACTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCGTCCATTTCGACAATTTCGCTCACTTTAGCGATAgtgt                           <  2:3268523/148‑1 (MQ=255)
                                                                                                                      gCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTc                          >  1:2570359/1‑148 (MQ=255)
                                                                                                                      gCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACAAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTc                          >  2:2756317/1‑148 (MQ=255)
                                                                                                                       cacaATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCg                         >  1:778571/1‑148 (MQ=255)
                                                                                                                          aaTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTg                      <  1:2203113/148‑1 (MQ=255)
                                                                                                                          aaTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTg                      >  2:1183749/1‑148 (MQ=255)
                                                                                                                               cGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGtt                 <  2:3428282/148‑1 (MQ=255)
                                                                                                                                 ttATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTAt               <  2:2056561/148‑1 (MQ=255)
                                                                                                                                  tATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATc              >  2:1399842/1‑148 (MQ=255)
                                                                                                                                   aTCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCa             <  2:1314765/148‑1 (MQ=255)
                                                                                                                                   aTCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCa             <  1:2157509/148‑1 (MQ=255)
                                                                                                                                   aTCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTATAGCGATAGTGTCGTTGAAGTTaaaa             >  2:514153/1‑145 (MQ=255)
                                                                                                                                     cGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGTAGTTATCAcc           >  1:1713820/1‑148 (MQ=255)
                                                                                                                                     cGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCAcc           <  1:145426/148‑1 (MQ=255)
                                                                                                                                     cGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCAcc           >  1:2875541/1‑148 (MQ=255)
                                                                                                                                         atcatAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGc       <  2:576290/148‑1 (MQ=255)
                                                                                                                                          tcatAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGTTGTTATCACCATGCa      <  1:1202820/148‑1 (MQ=255)
                                                                                                                                           catAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAg     >  1:2383387/1‑148 (MQ=255)
                                                                                                                                            atAGATATCTTCCATAATTTCCAGACCACTCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGa    <  2:2949253/148‑1 (MQ=255)
                                                                                                                                            atAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGa    <  2:3487796/148‑1 (MQ=255)
                                                                                                                                            atAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGa    <  1:2868479/148‑1 (MQ=255)
                                                                                                                                            atAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATAACCATGCAGa    >  1:2058527/1‑148 (MQ=255)
                                                                                                                                              agatATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAc  <  1:909430/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAC  >  CP009273/3258726‑3259015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑