Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,290,970 A→T 100% R176R (CGT→CGA)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,290,9700AT95.9% 455.8 / 8.2 147R176R (CGT→CGA) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base A (0/6);  new base T (73/68);  total (73/74)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 148 total aligned reads displayed.

CCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCA  >  CP009273/3290823‑3291114
                                                                                                                                                   |                                                                                                                                                
    aGCGCGCTGGCCCAGCGGCTAAAAAAGCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCGTGGGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGGGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCTGTCGaa                                                                                                                                              <  2:1949327/148‑1 (MQ=255)
    aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                              <  1:1120540/148‑1 (MQ=255)
    aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                              <  2:1949217/148‑1 (MQ=255)
     gcgcCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAAt                                                                                                                                             >  1:3090393/1‑148 (MQ=255)
         ccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgcc                                                                                                                                         >  2:1118403/1‑148 (MQ=255)
                cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAc                                                                                                                                  <  2:3104379/148‑1 (MQ=255)
                 aGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAcc                                                                                                                                 <  1:2823337/148‑1 (MQ=255)
                 aGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAcc                                                                                                                                 <  1:2823245/148‑1 (MQ=255)
                  gCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAccc                                                                                                                                <  2:1242052/148‑1 (MQ=255)
                     gCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGACTCGCCGCAAAACCCCAg                                                                                                                             >  2:2881260/1‑148 (MQ=255)
                     gCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAg                                                                                                                             >  2:367869/1‑148 (MQ=255)
                          aaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCACAACCCCAGCCGAg                                                                                                                        >  2:2982927/1‑148 (MQ=255)
                           aaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAgg                                                                                                                       <  2:3532571/148‑1 (MQ=255)
                            aaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGa                                                                                                                      <  1:1197223/148‑1 (MQ=255)
                            aaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGa                                                                                                                      <  1:1197870/148‑1 (MQ=255)
                               ccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACaa                                                                                                                   <  2:937940/148‑1 (MQ=255)
                                 gCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAg                                                                                                                 >  2:1353031/1‑148 (MQ=255)
                                 gCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAg                                                                                                                 <  1:1733457/148‑1 (MQ=255)
                                  cccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGc                                                                                                                >  1:1342883/1‑148 (MQ=255)
                                   ccTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGcc                                                                                                               <  1:3229227/148‑1 (MQ=255)
                                      gtgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCAt                                                                                                            <  1:1697858/148‑1 (MQ=255)
                                      gtgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCAt                                                                                                            <  1:1698163/148‑1 (MQ=255)
                                       tgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATa                                                                                                           >  2:2308372/1‑148 (MQ=255)
                                       tgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATa                                                                                                           >  2:1108547/1‑148 (MQ=255)
                                       tgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATa                                                                                                           <  1:793951/148‑1 (MQ=255)
                                       tgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATa                                                                                                           <  1:1967846/148‑1 (MQ=255)
                                        gCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATaa                                                                                                          <  1:3369403/148‑1 (MQ=255)
                                              ggTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCa                                                                                                    <  2:3347918/148‑1 (MQ=255)
                                              ggTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCa                                                                                                    <  2:1306260/148‑1 (MQ=255)
                                              ggTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCa                                                                                                    >  2:2446514/1‑148 (MQ=255)
                                               gTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAg                                                                                                   <  2:3024712/148‑1 (MQ=255)
                                                tATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGc                                                                                                  >  2:3094577/1‑148 (MQ=255)
                                                 aTGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCg                                                                                                 >  2:47400/1‑148 (MQ=255)
                                                     cAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGc                                                                                             <  2:1793277/148‑1 (MQ=255)
                                                       agttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCg                                                                                           <  2:406702/146‑1 (MQ=255)
                                                        tttCGACCTGTGCCTGCGTTTGCTGTGTTTCACGCACCCATTTGTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCgg                                                                                          <  2:2920259/148‑1 (MQ=255)
                                                        tttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCgg                                                                                          >  1:2513970/1‑148 (MQ=255)
                                                         ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                         <  2:2180159/148‑1 (MQ=255)
                                                         ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                         <  1:182603/148‑1 (MQ=255)
                                                         ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                         <  2:694033/148‑1 (MQ=255)
                                                             aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCCGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTa                                                                                     <  2:843497/148‑1 (MQ=255)
                                                             aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTa                                                                                     >  2:2914275/1‑148 (MQ=255)
                                                             aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTa                                                                                     <  1:2139560/148‑1 (MQ=255)
                                                             aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCAGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTa                                                                                     >  2:2586448/1‑148 (MQ=255)
                                                               cGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAac                                                                                   <  1:2076642/148‑1 (MQ=255)
                                                               cGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTGTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAac                                                                                   <  2:2903714/148‑1 (MQ=255)
                                                                 gTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAacac                                                                                 <  2:1212705/148‑1 (MQ=255)
                                                                   gCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCg                                                                               >  1:1608884/1‑148 (MQ=255)
                                                                   gCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCg                                                                               >  1:455187/1‑148 (MQ=255)
                                                                    ccTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCgg                                                                              <  1:2391122/148‑1 (MQ=255)
                                                                    ccTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCgg                                                                              >  2:2069064/1‑148 (MQ=255)
                                                                     cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGa                                                                             <  1:321641/148‑1 (MQ=255)
                                                                          tttGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGcc                                                                        <  2:2796751/148‑1 (MQ=255)
                                                                          tttGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGcc                                                                        >  1:1443506/1‑148 (MQ=255)
                                                                           ttGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGccc                                                                       <  1:512616/148‑1 (MQ=255)
                                                                             gCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCAt                                                                     <  1:3105767/148‑1 (MQ=255)
                                                                              cGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATc                                                                    >  2:3514193/1‑148 (MQ=255)
                                                                               ggTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATcc                                                                   <  1:557492/148‑1 (MQ=255)
                                                                                  gtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                                <  2:2722507/148‑1 (MQ=255)
                                                                                  gtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                                <  2:2723501/148‑1 (MQ=255)
                                                                                   tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                               >  1:3343975/1‑148 (MQ=255)
                                                                                   tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                               >  2:620257/1‑148 (MQ=255)
                                                                                   tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                               >  2:620290/1‑148 (MQ=255)
                                                                                     tCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATg                                                             <  1:1462968/148‑1 (MQ=255)
                                                                                     tCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATg                                                             >  1:2261726/1‑148 (MQ=255)
                                                                                       aCGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGcc                                                           <  2:2482055/148‑1 (MQ=255)
                                                                                        cGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCa                                                          >  1:739968/1‑148 (MQ=255)
                                                                                        cGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCa                                                          <  1:2966657/148‑1 (MQ=255)
                                                                                          cACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgc                                                        >  1:1026789/1‑148 (MQ=255)
                                                                                           aCCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgcg                                                       >  1:2589245/1‑148 (MQ=255)
                                                                                            cccATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgcgc                                                      >  1:746882/1‑148 (MQ=255)
                                                                                               aTTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCACAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCCGGTTTAACACCGGATTGCCCATCCAGAATGCCAGAGCAcc                                                   >  2:2279158/1‑148 (MQ=255)
                                                                                                 ttttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGc                                                 >  1:2386491/1‑148 (MQ=255)
                                                                                                 ttttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGc                                                 >  1:2386519/1‑148 (MQ=255)
                                                                                                  tttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGTCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCCGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGcc                                                <  2:2698595/148‑1 (MQ=255)
                                                                                                  tttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGcc                                                <  2:2695354/148‑1 (MQ=255)
                                                                                                     gCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCAt                                             <  1:54967/148‑1 (MQ=255)
                                                                                                      cACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATc                                            <  1:1787653/148‑1 (MQ=255)
                                                                                                      cACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATc                                            >  2:3442317/1‑148 (MQ=255)
                                                                                                        ccAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGa                                          >  1:2024876/1‑148 (MQ=255)
                                                                                                         cAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGac                                         >  1:2908039/1‑148 (MQ=255)
                                                                                                          aGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGaca                                        >  1:3512537/1‑148 (MQ=255)
                                                                                                               cGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGt                                   >  1:2128612/1‑148 (MQ=255)
                                                                                                               cGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGt                                   <  1:1839278/148‑1 (MQ=255)
                                                                                                                gCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGtt                                  <  2:2615832/148‑1 (MQ=255)
                                                                                                                  aaTCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTgg                                <  1:2312827/148‑1 (MQ=255)
                                                                                                                    tCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACACAGCCCATAAACACCAGCCTCGCCGGGGGTAACACCGGATTGCCCAGCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCg                              >  2:1864503/1‑148 (MQ=255)
                                                                                                                    tCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCg                              >  1:1604676/1‑148 (MQ=255)
                                                                                                                    tCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCg                              >  2:503452/1‑148 (MQ=255)
                                                                                                                       gCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCATCGCACCGCCCATCGACACCTGTTGGCGACg                           <  2:942541/148‑1 (MQ=255)
                                                                                                                        cAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGc                          >  2:583152/1‑148 (MQ=255)
                                                                                                                        cAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCAGCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGc                          >  2:583132/1‑148 (MQ=255)
                                                                                                                              cATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTccc                    >  1:1064283/1‑148 (MQ=255)
                                                                                                                                   ccAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCcgccgc               >  1:1930780/1‑148 (MQ=255)
                                                                                                                                         cGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCgg         <  2:28337/148‑1 (MQ=255)
                                                                                                                                             caccaGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGcc     <  1:1337996/148‑1 (MQ=255)
                                                                                                                                              accaGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCg    >  2:2855743/1‑148 (MQ=255)
                                                                                                                                              accaGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCg    >  1:92759/1‑148 (MQ=255)
                                                                                                                                               ccaGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGc   >  1:3523094/1‑148 (MQ=255)
                                                                                                                                                caGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGca  >  1:789051/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCA  >  CP009273/3290823‑3291114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑