Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,295,911 C→A 100% Q22K (CAG→AAG)  yhbV → U32 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,295,9110CA95.5% 463.4 / 12.6 156Q22K (CAG→AAG) yhbVU32 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (85/63);  total (89/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 161 total aligned reads displayed.

GGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCACGCTG  >  CP009273/3295766‑3296054
                                                                                                                                                 |                                                                                                                                               
ggatggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaag                                                                                                                                               >  2:931323/1‑148 (MQ=255)
  atggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaagca                                                                                                                                             >  2:876889/1‑148 (MQ=255)
    ggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAgg                                                                                                                                           <  2:1705517/148‑1 (MQ=255)
    ggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAgg                                                                                                                                           <  2:1459650/148‑1 (MQ=255)
    ggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAgg                                                                                                                                           <  2:2038966/148‑1 (MQ=255)
    ggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAgg                                                                                                                                           <  2:2038975/148‑1 (MQ=255)
    ggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAgg                                                                                                                                           <  2:2103162/148‑1 (MQ=255)
        aCGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGgccgc                                                                                                                                       <  2:3384231/148‑1 (MQ=255)
        aCGCTCGGGGCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGgccgc                                                                                                                                       <  1:1843932/148‑1 (MQ=255)
                gTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACcagca                                                                                                                               >  2:1560339/1‑148 (MQ=255)
                  cGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCagcagc                                                                                                                             >  2:1142439/1‑148 (MQ=255)
                  cGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCagcagc                                                                                                                             >  2:1142747/1‑148 (MQ=255)
                    aTGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAgcgc                                                                                                                           >  2:3224960/1‑148 (MQ=255)
                     tGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGcc                                                                                                                          >  1:2347146/1‑148 (MQ=255)
                      gTCCGACGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCg                                                                                                                         <  2:2717328/148‑1 (MQ=255)
                       tCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGa                                                                                                                        <  2:1702973/148‑1 (MQ=255)
                       tCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGa                                                                                                                        <  2:3463272/148‑1 (MQ=255)
                           aaGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTg                                                                                                                    >  2:1385699/1‑148 (MQ=255)
                           aaGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTg                                                                                                                    >  2:3151691/1‑148 (MQ=255)
                           aaGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTg                                                                                                                    <  1:3031397/148‑1 (MQ=255)
                            aGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGa                                                                                                                   >  1:1869992/1‑148 (MQ=255)
                            aGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGa                                                                                                                   <  2:2502926/148‑1 (MQ=255)
                            aGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGa                                                                                                                   >  1:3556841/1‑148 (MQ=255)
                            aGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGa                                                                                                                   <  1:2779340/148‑1 (MQ=255)
                             ggCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGTATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAt                                                                                                                  <  1:2514451/148‑1 (MQ=255)
                              gCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAtt                                                                                                                 <  1:2325256/148‑1 (MQ=255)
                                 cGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTAt                                                                                                              <  2:588594/148‑1 (MQ=255)
                                 cGCAAACCACTCTGGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTAt                                                                                                              <  2:1813264/148‑1 (MQ=255)
                                   cAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCt                                                                                                            >  2:251782/1‑148 (MQ=255)
                                     aaCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTg                                                                                                          <  1:1073775/148‑1 (MQ=255)
                                      aCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTCCTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTgg                                                                                                         >  1:885005/1‑148 (MQ=255)
                                      aCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTgg                                                                                                         >  1:885004/1‑148 (MQ=255)
                                        cACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTg                                                                                                       <  2:723065/148‑1 (MQ=255)
                                          ctctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGaa                                                                                                     <  1:1660206/148‑1 (MQ=255)
                                          ctctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGaa                                                                                                     <  1:1660117/148‑1 (MQ=255)
                                            ctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGc                                                                                                   >  1:807012/1‑148 (MQ=255)
                                            ctTGGCGCATATCACCGTAAATGGAAGTGAGAAAATCAAAGAAATATTCCTTAGGGCCAGTGCTGTGGTCCTGGCCAAAAGAGACCCTGGAAGAATTTTATAAGAAGGCCGCCACCACCAGCGCCGACGTGATTTATCTTGGTGAAGc                                                                                                   >  1:1133512/1‑148 (MQ=255)
                                               ggCGCATTTCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGt                                                                                                <  2:2920575/148‑1 (MQ=255)
                                                 cgcATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTAt                                                                                              >  2:2637804/1‑148 (MQ=255)
                                                    atatCACCTTTAATTGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGATGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTTTCTTGGTTAAGCTGTTTgca                                                                                           <  2:2929510/148‑1 (MQ=255)
                                                     tatCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcag                                                                                          >  1:2062894/1‑148 (MQ=255)
                                                     tatCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcag                                                                                          >  1:599536/1‑148 (MQ=255)
                                                      atCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcagc                                                                                         <  2:1971833/148‑1 (MQ=255)
                                                         aCCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAg                                                                                      <  1:3185181/148‑1 (MQ=255)
                                                         aCCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAg                                                                                      >  1:874814/1‑148 (MQ=255)
                                                           cGTAAATGGCAGTGAGAAAAGCACTGAAATATTCCTTAGGGCCAGTGCTGTGGAACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATATTGGTGAAGCGGTAAGCAGCAAGCg                                                                                    >  2:295516/1‑148 (MQ=255)
                                                            gTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGt                                                                                   >  1:2341217/1‑148 (MQ=255)
                                                             tAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTc                                                                                  >  1:3408416/1‑148 (MQ=255)
                                                                cgggCAGTGAGAAAAGCAATGACATAGTCCTGAGGGCCAGTGCTGTGGGACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCGTGGTGAAGCGGTATGCAGCAAGCGTCggg                                                                               <  1:1606904/146‑1 (MQ=255)
                                                                aTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCggg                                                                               <  1:1606866/148‑1 (MQ=255)
                                                                 tGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTCCTGGCCAAAAGAGACGCTGGACGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGc                                                                              >  1:159663/1‑148 (MQ=255)
                                                                   gCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCaa                                                                            >  1:656718/1‑148 (MQ=255)
                                                                   gCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCaa                                                                            <  1:2802132/148‑1 (MQ=255)
                                                                    cAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTCTGCAGCAAGCGTCGGGCAAc                                                                           >  2:419539/1‑148 (MQ=255)
                                                                    cAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCGGCAAGCGTCGGGCAAc                                                                           >  2:419408/1‑148 (MQ=255)
                                                                    cAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAAc                                                                           <  1:3222839/148‑1 (MQ=255)
                                                                       tGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCaa                                                                        >  1:1903017/1‑148 (MQ=255)
                                                                         agaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAg                                                                      <  1:239537/148‑1 (MQ=255)
                                                                         agaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAg                                                                      <  1:563661/148‑1 (MQ=255)
                                                                          gaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGt                                                                     <  1:1047424/148‑1 (MQ=255)
                                                                          gaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGt                                                                     <  2:534681/148‑1 (MQ=255)
                                                                           aaaaGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGtt                                                                    >  2:1380000/1‑148 (MQ=255)
                                                                            aaaGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTg                                                                   >  1:1746071/1‑148 (MQ=255)
                                                                                cAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGa                                                               >  2:2562654/1‑148 (MQ=255)
                                                                                cAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGa                                                               >  2:2562411/1‑148 (MQ=255)
                                                                                cAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGa                                                               <  2:3267994/148‑1 (MQ=255)
                                                                                cAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGa                                                               >  1:348666/1‑148 (MQ=255)
                                                                                cAATGAAATATTCCTTAGGGCCAGTGCTGAGGTACTGGCCAAAAGAGACGCTGGCAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGa                                                               >  2:2384837/1‑148 (MQ=255)
                                                                                    gAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActgg                                                           >  1:1289014/1‑148 (MQ=255)
                                                                                      aaTATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActggct                                                         <  2:1518202/148‑1 (MQ=255)
                                                                                            ccTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGAt                                                   <  2:1981923/148‑1 (MQ=255)
                                                                                              ttAGGGCCAGTGCTGTGGTCCTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATgg                                                 >  1:1663798/1‑148 (MQ=255)
                                                                                              ttAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATgg                                                 >  2:3544621/1‑148 (MQ=255)
                                                                                               tAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGc                                                >  1:1227999/1‑148 (MQ=255)
                                                                                               tAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGc                                                >  1:2324778/1‑148 (MQ=255)
                                                                                                aGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCa                                               >  2:1648351/1‑148 (MQ=255)
                                                                                                  ggCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaa                                             >  2:1026248/1‑148 (MQ=255)
                                                                                                      aGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcg                                         <  1:2792014/148‑1 (MQ=255)
                                                                                                      aGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcg                                         >  1:1108130/1‑148 (MQ=255)
                                                                                                      aGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcg                                         >  1:1720437/1‑148 (MQ=255)
                                                                                                       gTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgc                                        <  1:2174622/148‑1 (MQ=255)
                                                                                                       gTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgc                                        >  2:481471/1‑148 (MQ=255)
                                                                                                        tGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgct                                       >  2:2969796/1‑148 (MQ=255)
                                                                                                        tGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgct                                       >  1:1078951/1‑148 (MQ=255)
                                                                                                           tgtgGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtcgctcgc                                    >  2:2321479/1‑148 (MQ=255)
                                                                                                                 aCTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAg                              >  1:3204015/1‑148 (MQ=255)
                                                                                                                   tGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTg                            <  2:2546973/148‑1 (MQ=255)
                                                                                                                     gCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGt                          <  1:1833448/148‑1 (MQ=255)
                                                                                                                           agagACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAg                    >  2:252278/1‑148 (MQ=255)
                                                                                                                            gagaCGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGa                   >  2:1934688/1‑148 (MQ=255)
                                                                                                                            gagaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGa                   <  1:3088378/148‑1 (MQ=255)
                                                                                                                             agaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAt                  >  2:245864/1‑148 (MQ=255)
                                                                                                                             agaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAt                  >  2:3529691/1‑148 (MQ=255)
                                                                                                                              gaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAtt                 >  2:350530/1‑148 (MQ=255)
                                                                                                                                cGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATtgt               >  2:1545702/1‑148 (MQ=255)
                                                                                                                                 gCTGGAAGAATTTTATTAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATtgtg              <  1:881018/148‑1 (MQ=255)
                                                                                                                                    ggAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGctc           >  1:1371057/1‑148 (MQ=255)
                                                                                                                                        gaaTTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCa       >  1:1577144/1‑148 (MQ=255)
                                                                                                                                             ttATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCAcgctg  >  1:644995/1‑148 (MQ=255)
                                                                                                                                             ttATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCAcgctg  >  1:2428239/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCACGCTG  >  CP009273/3295766‑3296054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑