Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,306,165 C→A 39.4% V125F (GTT→TTT)  rbfA ← 30s ribosome binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,306,1650CA39.4% 281.8 / 221.8 223V125F (GTT→TTT) rbfA30s ribosome binding factor
Reads supporting (aligned to +/- strand):  ref base C (60/75);  new base A (45/43);  total (105/118)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

GGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAG  >  CP009273/3306019‑3306311
                                                                                                                                                  |                                                                                                                                                  
     gCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgacg                                                                                                                                              >  1:3400396/1‑148 (MQ=255)
        ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttc                                                                                                                                           <  2:2391539/148‑1 (MQ=255)
        ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttc                                                                                                                                           <  1:1668710/148‑1 (MQ=255)
        ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttc                                                                                                                                           >  1:517365/1‑148 (MQ=255)
         tatatataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttct                                                                                                                                          >  1:401188/1‑148 (MQ=255)
          atatataCGGTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttctt                                                                                                                                         <  2:2106040/148‑1 (MQ=255)
           tatataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGttcttc                                                                                                                                        >  1:2514908/1‑148 (MQ=255)
            atataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCg                                                                                                                                       >  1:730448/1‑148 (MQ=255)
                  gTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGt                                                                                                                                 <  1:3193029/148‑1 (MQ=255)
                   tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                                                                                                                >  2:345138/1‑148 (MQ=255)
                   tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                                                                                                                >  2:2561287/1‑148 (MQ=255)
                   tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                                                                                                                >  2:1909766/1‑148 (MQ=255)
                     tCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTg                                                                                                                              >  1:1865863/1‑148 (MQ=255)
                        cTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGAcc                                                                                                                           >  1:1121946/1‑148 (MQ=255)
                        cTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGAcc                                                                                                                           >  1:1725511/1‑148 (MQ=255)
                         tttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCa                                                                                                                          <  2:1788554/148‑1 (MQ=255)
                         tttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCa                                                                                                                          <  2:27282/148‑1 (MQ=255)
                         tttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCa                                                                                                                          <  2:99006/148‑1 (MQ=255)
                           tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                        <  1:51319/148‑1 (MQ=255)
                           tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                        <  1:1006242/148‑1 (MQ=255)
                           tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                        <  1:1582570/148‑1 (MQ=255)
                             tGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCt                                                                                                                      <  2:2787973/148‑1 (MQ=255)
                              gCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTg                                                                                                                     <  1:793978/148‑1 (MQ=255)
                              gCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTg                                                                                                                     <  1:2849212/148‑1 (MQ=255)
                               cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                    >  2:924779/1‑148 (MQ=255)
                               cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                    <  1:188622/148‑1 (MQ=255)
                               cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                    <  1:2477992/148‑1 (MQ=255)
                                 gcgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTc                                                                                                                  >  1:1763969/1‑148 (MQ=255)
                                 gcgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTc                                                                                                                  >  2:2329197/1‑148 (MQ=255)
                                  cgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCa                                                                                                                 >  2:3474296/1‑148 (MQ=255)
                                    cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                                                                                                               <  1:2459055/148‑1 (MQ=255)
                                      tCGTTGCTGGGCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAg                                                                                                             >  1:2710964/1‑148 (MQ=255)
                                       cGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAgg                                                                                                            >  2:1740088/1‑148 (MQ=255)
                                       cGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAgg                                                                                                            <  2:3086086/148‑1 (MQ=255)
                                        gTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGt                                                                                                           <  1:3033211/148‑1 (MQ=255)
                                          tGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                                                                                                         >  1:1486982/1‑148 (MQ=255)
                                           gCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTg                                                                                                        <  1:2959247/148‑1 (MQ=255)
                                           gCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTg                                                                                                        <  1:3223477/148‑1 (MQ=255)
                                             tGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGAc                                                                                                      <  2:1656998/148‑1 (MQ=255)
                                               gACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                    >  2:1363728/1‑148 (MQ=255)
                                               gACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                    >  1:291478/1‑148 (MQ=255)
                                               gACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                    >  1:1965720/1‑148 (MQ=255)
                                                aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                   <  1:3036922/148‑1 (MQ=255)
                                                aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                   <  1:151764/148‑1 (MQ=255)
                                                   tACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCGGGTTTGACATgcgc                                                                                                >  1:2721867/1‑148 (MQ=255)
                                                      ccTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATc                                                                                             >  2:861266/1‑148 (MQ=255)
                                                       cTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATcc                                                                                            >  1:277552/1‑148 (MQ=255)
                                                        tGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATccc                                                                                           >  2:380245/1‑148 (MQ=255)
                                                        tGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATccc                                                                                           <  1:3485301/148‑1 (MQ=255)
                                                          aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCtt                                                                                         >  2:589005/1‑148 (MQ=255)
                                                               tATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAcc                                                                                    <  2:3026670/148‑1 (MQ=255)
                                                               tATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAcc                                                                                    <  1:1692759/148‑1 (MQ=255)
                                                                 tCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCag                                                                                  >  2:3197338/1‑148 (MQ=255)
                                                                 tCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCag                                                                                  >  2:2098431/1‑148 (MQ=255)
                                                                  ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCaga                                                                                 <  1:2959863/148‑1 (MQ=255)
                                                                      caacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGt                                                                             <  1:1215213/148‑1 (MQ=255)
                                                                          aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTc                                                                         <  2:3290684/148‑1 (MQ=255)
                                                                           aaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCg                                                                        >  2:468036/1‑148 (MQ=255)
                                                                              cGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTag                                                                     <  1:780469/148‑1 (MQ=255)
                                                                                   ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAgaagaa                                                                >  1:1090128/1‑148 (MQ=255)
                                                                                     aaTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAgg                                                              <  1:139015/148‑1 (MQ=255)
                                                                                     aaTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAgg                                                              <  1:138979/148‑1 (MQ=255)
                                                                                       tGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTc                                                            <  2:286515/148‑1 (MQ=255)
                                                                                        gTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCa                                                           >  1:1026823/1‑148 (MQ=255)
                                                                                         tCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAg                                                          <  2:2472835/148‑1 (MQ=255)
                                                                                          cgcgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGt                                                         <  2:2666501/148‑1 (MQ=255)
                                                                                          cgcgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGt                                                         >  2:2015355/1‑148 (MQ=255)
                                                                                            cgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTc                                                       <  1:1583106/148‑1 (MQ=255)
                                                                                            cgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTa                                                                      <  1:2647232/133‑1 (MQ=255)
                                                                                            cgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTa                                                                      >  2:2647232/1‑133 (MQ=255)
                                                                                               ccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCgg                                                    >  2:639348/1‑148 (MQ=255)
                                                                                               ccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCgg                                                    >  2:331955/1‑148 (MQ=255)
                                                                                               ccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCgg                                                    >  2:2428377/1‑148 (MQ=255)
                                                                                               ccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCgg                                                    >  1:2053561/1‑148 (MQ=255)
                                                                                               ccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCgg                                                    >  1:961465/1‑148 (MQ=255)
                                                                                                 gcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCa                                                  <  1:3439558/148‑1 (MQ=255)
                                                                                                    acgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGa                                               >  1:607153/1‑148 (MQ=255)
                                                                                                    acgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTCCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGa                                               <  2:2827013/148‑1 (MQ=255)
                                                                                                     cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGAt                                              <  1:1533832/148‑1 (MQ=255)
                                                                                                           ggACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAg                                        >  1:3280579/1‑148 (MQ=255)
                                                                                                           ggACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAg                                        >  2:3029488/1‑148 (MQ=255)
                                                                                                              cgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGgcg                                     <  1:2561287/148‑1 (MQ=255)
                                                                                                                  tCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATc                                 >  1:2890426/1‑148 (MQ=255)
                                                                                                                   cATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCg                                >  2:1667971/1‑148 (MQ=255)
                                                                                                                      tAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCtt                             >  2:1249019/1‑148 (MQ=255)
                                                                                                                          cctcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCATGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTccc                         <  1:527719/148‑1 (MQ=255)
                                                                                                                              ctTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcagc                     <  2:730448/148‑1 (MQ=255)
                                                                                                                               ttGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcagca                    >  1:922249/1‑148 (MQ=255)
                                                                                                                                 gCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAgg                  >  2:230861/1‑148 (MQ=255)
                                                                                                                                   tGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCt                >  2:1738474/1‑148 (MQ=255)
                                                                                                                                    gtcgtcCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTg               <  1:2329197/148‑1 (MQ=255)
                                                                                                                                    gtcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTg               <  1:763690/148‑1 (MQ=255)
                                                                                                                                       gtcCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCgg            <  2:1996209/148‑1 (MQ=255)
                                                                                                                                       gtcCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCgg            <  1:231654/148‑1 (MQ=255)
                                                                                                                                       gtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCgg            <  2:2514908/148‑1 (MQ=255)
                                                                                                                                         ccGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGAt          >  2:292954/1‑148 (MQ=255)
                                                                                                                                           gggTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGa        <  1:1909766/148‑1 (MQ=255)
                                                                                                                                              ttAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAc     >  1:1791451/1‑148 (MQ=255)
                                                                                                                                                 acacGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAg  >  2:2272697/1‑148 (MQ=255)
                                                                                                                                                 acacGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAg  >  1:1437781/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAG  >  CP009273/3306019‑3306311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑