Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,334 C→A 93.1% I141I (ATC→ATA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,3340CA93.1% 430.6 / 22.5 155I141I (ATC→ATA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base A (60/84);  total (65/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACG  >  CP009273/279189‑279478
                                                                                                                                                 |                                                                                                                                                
gCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCACCGACGCGGCGATAgt                                                                                                                                                >  2:2662745/1‑148 (MQ=255)
gCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgt                                                                                                                                                >  1:802272/1‑148 (MQ=255)
gCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgt                                                                                                                                                <  2:1576275/148‑1 (MQ=255)
gCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgt                                                                                                                                                >  1:2891023/1‑148 (MQ=255)
gCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGAATTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgt                                                                                                                                                >  1:802352/1‑148 (MQ=255)
 cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                               <  2:1493252/148‑1 (MQ=255)
 cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                               <  2:1493224/148‑1 (MQ=255)
    gcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGttt                                                                                                                                            >  1:1757914/1‑148 (MQ=255)
     cagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTc                                                                                                                                           <  2:3220056/148‑1 (MQ=255)
     cagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTc                                                                                                                                           <  2:3219980/148‑1 (MQ=255)
       gatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgc                                                                                                                                         >  2:2139317/1‑148 (MQ=255)
        atgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTCTGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgcc                                                                                                                                        >  2:2560377/1‑148 (MQ=255)
        atgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgcc                                                                                                                                        >  2:2560335/1‑148 (MQ=255)
         tgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgccg                                                                                                                                       >  1:2218943/1‑148 (MQ=255)
              gcggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGa                                                                                                                                  <  1:1398940/148‑1 (MQ=255)
              gcggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGa                                                                                                                                  <  1:1398944/148‑1 (MQ=255)
                      gAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTcc                                                                                                                          <  2:2192929/148‑1 (MQ=255)
                      gAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTcc                                                                                                                          <  2:1197839/148‑1 (MQ=255)
                      gAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTcc                                                                                                                          <  2:2935225/148‑1 (MQ=255)
                        aGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCt                                                                                                                        >  1:2184911/1‑148 (MQ=255)
                        aGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCt                                                                                                                        >  1:3373630/1‑148 (MQ=255)
                        aGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCt                                                                                                                        >  1:3355993/1‑148 (MQ=255)
                          gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGc                                                                                                                      >  2:1945815/1‑148 (MQ=255)
                          gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGc                                                                                                                      >  2:1020437/1‑148 (MQ=255)
                          gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGc                                                                                                                      >  2:1020498/1‑148 (MQ=255)
                           agaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGcc                                                                                                                     <  1:2909508/148‑1 (MQ=255)
                             aTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGa                                                                                                                   >  2:2670642/1‑148 (MQ=255)
                                   cGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggc                                                                                                             >  1:2181711/1‑148 (MQ=255)
                                          gCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGt                                                                                                      >  1:2175638/1‑148 (MQ=255)
                                          gCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGt                                                                                                      <  1:84469/148‑1 (MQ=255)
                                          gCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGt                                                                                                      <  1:2172445/148‑1 (MQ=255)
                                           cGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTg                                                                                                     <  1:194976/148‑1 (MQ=255)
                                           cGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTg                                                                                                     <  1:196584/148‑1 (MQ=255)
                                            gggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGa                                                                                                    <  2:3418642/148‑1 (MQ=255)
                                            gggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGa                                                                                                    <  2:3244240/148‑1 (MQ=255)
                                             ggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAt                                                                                                   >  1:2315524/1‑148 (MQ=255)
                                             ggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAt                                                                                                   <  2:339616/148‑1 (MQ=255)
                                                   cGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTTCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGt                                                                                             >  2:1159188/1‑148 (MQ=255)
                                                   cGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGt                                                                                             <  1:1125328/148‑1 (MQ=255)
                                                    gTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                            <  1:3180875/148‑1 (MQ=255)
                                                     ttCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAg                                                                                           >  1:1886480/1‑148 (MQ=255)
                                                     ttCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAg                                                                                           >  1:1888961/1‑148 (MQ=255)
                                                         cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAc                                                                                       <  2:1115579/148‑1 (MQ=255)
                                                         cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAc                                                                                       <  2:866316/148‑1 (MQ=255)
                                                         cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAc                                                                                       >  1:2384494/1‑148 (MQ=255)
                                                           gCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCt                                                                                     >  2:1865400/1‑148 (MQ=255)
                                                            ccTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTg                                                                                    <  1:1315842/148‑1 (MQ=255)
                                                                  tCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaa                                                                              <  2:2325386/148‑1 (MQ=255)
                                                                  tCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaa                                                                              >  1:3397056/1‑148 (MQ=255)
                                                                   cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                             <  2:2221825/148‑1 (MQ=255)
                                                                   cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                             >  1:2075326/1‑148 (MQ=255)
                                                                      cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                          <  2:1316370/148‑1 (MQ=255)
                                                                         tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                       <  2:393176/148‑1 (MQ=255)
                                                                               cGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGcc                                                                 >  2:3235497/1‑148 (MQ=255)
                                                                                  cGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacc                                                              <  2:1925732/148‑1 (MQ=255)
                                                                                   gggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacca                                                             <  1:588891/148‑1 (MQ=255)
                                                                                    ggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAt                                                            >  1:649447/1‑148 (MQ=255)
                                                                                     gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGTTGCCCGCCACCATg                                                           <  1:2394494/148‑1 (MQ=255)
                                                                                     gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGTTGCCCGCCACCATg                                                           <  1:2494259/148‑1 (MQ=255)
                                                                                       gcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAt                                                         <  1:1429203/148‑1 (MQ=255)
                                                                                           gCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcg                                                     >  2:580085/1‑148 (MQ=255)
                                                                                           gCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcg                                                     <  1:1354259/148‑1 (MQ=255)
                                                                                            cAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcgc                                                    <  1:1450298/148‑1 (MQ=255)
                                                                                             aGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCt                                                   <  2:3395201/148‑1 (MQ=255)
                                                                                                  acgcacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcg                                              <  1:2218846/148‑1 (MQ=255)
                                                                                                    gcacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcgcg                                            <  1:2554525/148‑1 (MQ=255)
                                                                                                    gcacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcgcg                                            <  2:1585301/148‑1 (MQ=255)
                                                                                                    gcacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGAGCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcgcg                                            <  2:2281907/148‑1 (MQ=255)
                                                                                                    gcacgGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcgcg                                            <  2:496077/148‑1 (MQ=255)
                                                                                                      acgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGAt                                          >  1:3217715/1‑148 (MQ=255)
                                                                                                      acgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGAt                                          >  1:3556408/1‑148 (MQ=255)
                                                                                                      acgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGAt                                          <  1:814249/148‑1 (MQ=255)
                                                                                                      acgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGAt                                          <  1:2351307/148‑1 (MQ=255)
                                                                                                        ggTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGc                                        <  1:316570/148‑1 (MQ=255)
                                                                                                         gTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCa                                       >  1:953847/1‑148 (MQ=255)
                                                                                                         gTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCa                                       >  1:2261650/1‑148 (MQ=255)
                                                                                                            tGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGa                                    >  1:1113702/1‑148 (MQ=255)
                                                                                                            tGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGa                                    >  1:1882531/1‑148 (MQ=255)
                                                                                                              ttCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGAcc                                  >  1:2481049/1‑148 (MQ=255)
                                                                                                                    tCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGa                            >  2:1523322/1‑148 (MQ=255)
                                                                                                                     cccTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGAc                           <  2:1239634/148‑1 (MQ=255)
                                                                                                                       cTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTa                         <  2:2184767/148‑1 (MQ=255)
                                                                                                                       cTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTa                         <  1:692267/148‑1 (MQ=255)
                                                                                                                       cTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTa                         <  1:692667/148‑1 (MQ=255)
                                                                                                                        tGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTAt                        <  1:1029999/148‑1 (MQ=255)
                                                                                                                            gTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTg                    <  2:915754/148‑1 (MQ=255)
                                                                                                                            gTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTg                    <  2:1632994/148‑1 (MQ=255)
                                                                                                                             tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTgg                   <  2:1244075/148‑1 (MQ=255)
                                                                                                                             tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTgg                   <  2:1410270/148‑1 (MQ=255)
                                                                                                                             tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTgg                   <  2:3443217/148‑1 (MQ=255)
                                                                                                                                cGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTCTTCTGGTGc                >  2:2891903/1‑148 (MQ=255)
                                                                                                                                cGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGc                <  2:2218940/148‑1 (MQ=255)
                                                                                                                                    acgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcggg            >  1:3541625/1‑148 (MQ=255)
                                                                                                                                    acgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcggg            <  1:3210746/148‑1 (MQ=255)
                                                                                                                                    acgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcggg            >  1:1194758/1‑148 (MQ=255)
                                                                                                                                     cgacgCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggc           >  2:110456/1‑148 (MQ=255)
                                                                                                                                      gacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcg          <  2:2078524/148‑1 (MQ=255)
                                                                                                                                      gacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcg          <  2:2078785/148‑1 (MQ=255)
                                                                                                                                       acgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcgg         >  2:1209017/1‑148 (MQ=255)
                                                                                                                                              gATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACg  <  2:3355993/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACG  >  CP009273/279189‑279478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑