Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,334,687 G→T 100% A21A (GCG→GCT)  kdsD → D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,334,6870GT96.8% 685.0 / 11.5 219A21A (GCG→GCT) kdsDD‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (6/1);  new base T (113/99);  total (119/100)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 219 total aligned reads displayed.

ATTATTAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGC  >  CP009273/3334542‑3334827
                                                                                                                                                 |                                                                                                                                            
attattAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTAt                                                                                                                                            >  2:1428826/1‑148 (MQ=255)
   attaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGa                                                                                                                                         <  2:1584220/148‑1 (MQ=255)
          ggCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGaa                                                                                                                                  <  1:1482447/148‑1 (MQ=255)
            cGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATg                                                                                                                                <  1:2438893/148‑1 (MQ=255)
            cGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATg                                                                                                                                >  2:1662737/1‑148 (MQ=255)
            cGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATg                                                                                                                                >  2:3047135/1‑148 (MQ=255)
            cGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATg                                                                                                                                >  1:625436/1‑148 (MQ=255)
             ggATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGc                                                                                                                               <  2:3156890/148‑1 (MQ=255)
               aTTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCt                                                                                                                             <  1:365841/148‑1 (MQ=255)
               aTTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCt                                                                                                                             <  1:364729/148‑1 (MQ=255)
                tttATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTg                                                                                                                            <  2:2404388/148‑1 (MQ=255)
                    tCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCgg                                                                                                                        >  2:2811638/1‑148 (MQ=255)
                     cGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGa                                                                                                                       >  2:1785898/1‑148 (MQ=255)
                      gTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAg                                                                                                                      >  1:2969856/1‑148 (MQ=255)
                        aTGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCt                                                                                                                    >  1:3224150/1‑148 (MQ=255)
                        aTGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCt                                                                                                                    >  2:2180991/1‑148 (MQ=255)
                          ggctggcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTg                                                                                                                  >  2:1030702/1‑148 (MQ=255)
                               gcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCaa                                                                                                             <  2:121606/148‑1 (MQ=255)
                               gcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCaa                                                                                                             <  1:1741175/148‑1 (MQ=255)
                                cGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAAt                                                                                                            <  1:2363669/148‑1 (MQ=255)
                                   tgttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACa                                                                                                         >  1:2058157/1‑148 (MQ=255)
                                   tgttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACa                                                                                                         <  1:130849/148‑1 (MQ=255)
                                   tgttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACa                                                                                                         <  1:2994853/148‑1 (MQ=255)
                                      tgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACatca                                                                                                      <  2:2964442/148‑1 (MQ=255)
                                       gtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACatcaa                                                                                                     <  2:1507194/148‑1 (MQ=255)
                                         aCTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACatcaatc                                                                                                   <  1:65763/148‑1 (MQ=255)
                                           tGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAg                                                                                                 >  1:1292597/1‑148 (MQ=255)
                                           tGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAg                                                                                                 >  2:2040139/1‑148 (MQ=255)
                                            ggTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGa                                                                                                <  1:3368806/148‑1 (MQ=255)
                                              ttATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAAt                                                                                              <  1:1348271/148‑1 (MQ=255)
                                              ttATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAAt                                                                                              <  1:1496870/148‑1 (MQ=255)
                                                 tCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTc                                                                                           >  1:1846872/1‑148 (MQ=255)
                                                  cGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCa                                                                                          >  2:404565/1‑148 (MQ=255)
                                                  cGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCa                                                                                          >  2:583685/1‑148 (MQ=255)
                                                   gCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCAc                                                                                         >  1:792450/1‑148 (MQ=255)
                                                    ccAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACg                                                                                        >  1:2665686/1‑148 (MQ=255)
                                                        tACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTg                                                                                    >  2:507387/1‑148 (MQ=255)
                                                           tCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCt                                                                                 <  2:2159224/148‑1 (MQ=255)
                                                           tCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCt                                                                                 <  1:3213945/148‑1 (MQ=255)
                                                           tCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTGTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCt                                                                                 <  2:2100687/148‑1 (MQ=255)
                                                            cGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtg                                                                                >  2:326480/1‑148 (MQ=255)
                                                            cGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtg                                                                                >  2:2123219/1‑148 (MQ=255)
                                                            cGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtg                                                                                >  2:461024/1‑148 (MQ=255)
                                                               tGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGa                                                                             >  2:1884801/1‑148 (MQ=255)
                                                               tGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGa                                                                             <  2:609280/148‑1 (MQ=255)
                                                                 aaTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaa                                                                           >  2:2043498/1‑148 (MQ=255)
                                                                 aaTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaa                                                                           <  1:2630756/148‑1 (MQ=255)
                                                                 aaTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaa                                                                           >  2:812531/1‑148 (MQ=255)
                                                                 aaTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaa                                                                           >  2:1029605/1‑148 (MQ=255)
                                                                  aTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaaa                                                                          >  1:1604105/1‑148 (MQ=255)
                                                                   tAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAg                                                                         >  2:2851643/1‑148 (MQ=255)
                                                                     aCTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGAt                                                                       >  2:3310086/1‑148 (MQ=255)
                                                                     aCTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGAt                                                                       >  2:894019/1‑148 (MQ=255)
                                                                        ggAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGtt                                                                    <  1:3309670/148‑1 (MQ=255)
                                                                        ggAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGtt                                                                    <  1:591934/148‑1 (MQ=255)
                                                                         gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTc                                                                   >  1:221805/1‑148 (MQ=255)
                                                                         gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTc                                                                   >  2:385984/1‑148 (MQ=255)
                                                                         gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTc                                                                   >  2:2308680/1‑148 (MQ=255)
                                                                           aaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTg                                                                 >  1:770022/1‑148 (MQ=255)
                                                                           aaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTg                                                                 <  1:1937312/148‑1 (MQ=255)
                                                                           aaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTg                                                                 <  1:361574/148‑1 (MQ=255)
                                                                            aCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTgg                                                                >  2:109867/1‑148 (MQ=255)
                                                                             cGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGgt                                                               >  2:2219738/1‑148 (MQ=255)
                                                                                 ttATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaa                                                           >  2:2282413/1‑148 (MQ=255)
                                                                                  tATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                          <  2:2969856/148‑1 (MQ=255)
                                                                                  tATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                          <  2:774030/148‑1 (MQ=255)
                                                                                   aTGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAg                                                         >  2:604420/1‑148 (MQ=255)
                                                                                      tCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGa                                                      <  1:507387/148‑1 (MQ=255)
                                                                                      tCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGa                                                      <  1:910250/148‑1 (MQ=255)
                                                                                       cGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGaa                                                     >  1:3109875/1‑148 (MQ=255)
                                                                                           cGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGtt                                                 >  1:2848636/1‑148 (MQ=255)
                                                                                               gagTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGACAGTTgtcg                                             >  1:2561390/1‑148 (MQ=255)
                                                                                                agTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgt                                            >  2:1109029/1‑148 (MQ=255)
                                                                                                agTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgt                                            >  1:1037623/1‑148 (MQ=255)
                                                                                                 gTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgtc                                           <  2:552825/148‑1 (MQ=255)
                                                                                                 gTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgtcgtc                                           <  1:2449936/148‑1 (MQ=255)
                                                                                                      aaCCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAACAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATggg                                      >  2:2930492/1‑148 (MQ=255)
                                                                                                       aCCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATgggg                                     <  1:2938273/148‑1 (MQ=255)
                                                                                                        ccGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGa                                    >  1:652567/1‑148 (MQ=255)
                                                                                                        ccGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGa                                    >  1:653058/1‑148 (MQ=255)
                                                                                                        ccGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGa                                    >  1:2153910/1‑148 (MQ=255)
                                                                                                         cGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGAt                                   <  1:2897400/148‑1 (MQ=255)
                                                                                                         cGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGAGTAAAGGGAAAGTTGTCGTCATGGGGAt                                   <  1:1122675/148‑1 (MQ=255)
                                                                                                            gTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATggg                                >  1:1216136/1‑148 (MQ=255)
                                                                                                            gTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATggg                                >  2:2743877/1‑148 (MQ=255)
                                                                                                             ttttGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGa                               >  2:2474606/1‑148 (MQ=255)
                                                                                                              tttGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGaa                              >  1:2808484/1‑148 (MQ=255)
                                                                                                                  aCTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATc                          <  2:153335/148‑1 (MQ=255)
                                                                                                                  aCTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATc                          <  2:1381274/148‑1 (MQ=255)
                                                                                                                         gcaagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCat                   <  2:212831/148‑1 (MQ=255)
                                                                                                                              caGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTgg              >  1:1562093/1‑148 (MQ=255)
                                                                                                                                  tAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGa          <  2:3224150/148‑1 (MQ=255)
                                                                                                                                  tAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGa          <  2:3420223/148‑1 (MQ=255)
                                                                                                                                   aaaGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCATTCAGAATTTCACGCTTGCCGGTGAAAAGATGTTCTGGTGTCATGGGAACGTTGTCGTCCTGGGGATGGGAAGATCGGGGCATATTGGGCGac         >  2:2327263/1‑147 (MQ=255)
                                                                                                                                     agaagTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaa       <  2:354463/148‑1 (MQ=255)
                                                                                                                                     agaagTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaa       >  1:1897239/1‑148 (MQ=255)
                                                                                                                                         gTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATgg   <  1:1662737/148‑1 (MQ=255)
                                                                                                                                         gTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATgg   <  2:2216040/148‑1 (MQ=255)
                                                                                                                                         gTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATgg   <  1:3047163/148‑1 (MQ=255)
                                                                                                                                          tCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATggc  >  1:1750160/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
ATTATTAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGC  >  CP009273/3334542‑3334827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑